[en] The distribution of iron among the two binding sites of transferrin was studied by isoelectric focusing in 41 patients with a variety of disorders of iron metabolism and erythropoiesis. The proportion of diferric and the two monoferric transferrins were very close to the values predicted from the transferring saturation. It is concluded that iron distribution on transferrin is random or close to random within the experimental error in patients with a variety of clinical disorders.
Disciplines :
Hematology
Author, co-author :
Beguin, Yves ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
Huebers, H.
Finch, C. A.
Language :
English
Title :
Random distribution of iron among the two binding sites of transferrin in patients with various hematologic disorders.
Huebers, Finch (1987) Transferrin and transferrin receptors. Physiol Rev 67:520-582.
Aisen, Leibman, Zweier (1978) Stoichiometric and site characteristics of the binding of iron to human transferrin. J Biol Chem 253:1930-1937.
Morgan, Huebers, Finch (1978) Differences between the binding sites for iron binding and release in human and rat transferrin. Blood 52:1219-1228.
Baldwin, De Sousa (1981) The effect of salts on the kinetics of iron release from N-terminal and C-terminal monoferric transferrins. Biochem Biophys Res Commun 99:1101-1107.
Ghasteen, Williams (1981) The influence of pH on the equilibrium distribution of iron between the metal-binding sites of human transferrin. Biochem J 193:717-727.
Delaney, Morgan, Morgan (1982) Chemical, but not functional, differences between the iron-binding sites of rabbit transferrin. Biochim Biophys Acta 701:295-304.
Williams, Chasteen, Moreton (1982) The effect of salt concentration on the iron-binding properties of human transferrin. Biochem J 201:527-532.
Fletcher, Huehns (1968) Function of transferrin. Nature 218:1211-1214.
Harris, Aisen (1975) Iron-donating properties of transferrin. Biochemistry 14:262-268.
Van Baarlen, Brouwer, Leibman, Aisen (1980) Evidence for the functional heterogeneity of the two sites of transferrin in vitro. Br J Haematol 46:417-426.
Van der Heul, Kroos, Van Noort, Van Eijk (1981) No functional difference of the two iron-binding sites of human transferrin in vitro. Clin Sci 60:185-190.
Groen, Hendricksen, Young, Leibman, Aisen (1982) Molecular ferrokinetics in the rabbit. Br J Haematol 50:43-53.
Heaphy, Williams (1982) Removal of iron from diferric rabbit serum transferrin by rabbit reticulocytes. Biochem J 205:619-621.
Huebers, Josephson, Huebers, Csiba, Finch (1981) Uptake and release of iron from human transferrin. Proc Natl Acad Sci USA , second edition; 78:2572-2576.
Huebers, Csiba, Huebers, Finch (1983) Competitive advantage of diferric transferrin in delivering iron to reticulocytes. Proc Natl Acad Sci USA , second edition; 80:300-304.
Huebers, Huebers, Csiba, Finch (1984) Heterogeneity of the plasma iron pool: explanation of the Fletcher-Huehns phenomenon. Am J Physiol 247:R280-R283.
Huebers, Uvelli, Celada, Josephson, Finch (1982) Basis of plasma iron exchange in the rabbit. J Clin Invest 70:769-779.
Huebers, Bauer, Huebers, Csiba, Finch (1981) The behavior of transferrin iron in the rat. Blood 57:218-228.
Huebers, Josephson, Huebers, Csiba, Finch (1984) Occupancy of the iron binding sites of human transferrin. Proc Natl Acad Sci USA , second edition; 81:4326-4330.
DiRusso, Check, Hunter (1985) Quantitation of apo-, mono-, and diferric transferrin by polyacrylamide gradient gel electrophoresis in patients with disorders of iron metabolism. Blood 66:1445-1451.
Leibman, Aisen (1979) Distribution of iron between the binding sites of transferrin in serum: methods and results in normal human subjects. Blood 53:1058-1065.
Williams, Moreton (1980) The distribution of iron between the metalbinding sites of transferrin in human serum. Biochem J 185:483-488.
Zak, Aisen (1986) Nonrandom distribution of iron in circulating human transferrin. Blood 68:157-161.
Van Eijk, van Noort (1986) A non-random distribution of transferrin iron in fresh human sera. Clin Chim Acta 157:299-304.
ICSH Expert Panel on Iron (1978) Recommendations for measurement of serum iron in human blood. British Journal of Haematology 38:291-294.
ICSH Expert Panel on Iron (1978) The measurement of total and unsaturated iron-binding capacity in serum. British Journal of Haematology 38:281-287.
Huebers, Csiba, Josephson, Huebers, Finch (1981) Interaction of human diferric transferrin with reticulocytes. Proc Natl Acad Sci USA , second edition; 78:621-625.
Huebers, Eng, Josephson (1987) The measurement of plasma iron and transferrin iron binding capacity by an immunoprecipitation method. Clin Chem 33:273-277.
Gazzola, Huebers, Sayers, MacPhail, Eng, Finch (1985) Transferrin saturation, plasma iron turnover, and transferrin uptake in normal humans. Blood 66:935-939.
