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Soluble and cell-associated transferrin receptor in lung cancer.
Dowlati, A.; Loo; Bury, Thierry et al.
1997In British Journal of Cancer, 75 (12), p. 1802-6
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Keywords :
Aged; Analysis of Variance; Bronchoalveolar Lavage Fluid; Bronchoscopy; Carcinoma, Non-Small-Cell Lung/blood/diagnosis/pathology; Carcinoma, Small Cell/blood/diagnosis/pathology; Data Interpretation, Statistical; Diagnosis, Differential; Enzyme-Linked Immunosorbent Assay; Female; Fiber Optic Technology; Humans; Lung/pathology; Lung Diseases, Obstructive/blood/diagnosis; Lung Neoplasms/blood/diagnosis/pathology; Male; Middle Aged; Neoplasm Staging; Phosphopyruvate Hydratase/analysis; Pneumonia/blood/diagnosis; Receptors, Transferrin/analysis/blood; Sensitivity and Specificity; Solubility
Abstract :
[en] The expression of transferrin receptor (TfR) has been identified in many malignant tumours. In lung cancer, lymphoma and breast cancer, it has been shown that the expression of TfR correlates with tumour differentiation, probably implying some prognostic value. A soluble form of TfR (sTfR) in human serum has been shown to be proportional to the number of cellular TfRs. Based on these data we examined the utility of measuring sTfR in the serum and bronchoalveolar lavage (BAL) fluid of patients with lung cancer (n = 32) and patients with chronic obstructive pulmonary disease (n = 22). BAL fluid was centrifuged to separate the supernatant from the cellular component. Cells were lysed in a detergent and cell-associated TfR was measured by enzyme-linked immunosorbent assay (ELISA) and expressed as ng 10(-6) cells in this cellular component. There was no difference in serum sTfR between the cancer and chronic obstructive pulmonary disease (COPD) groups. A higher level of cell-associated TfR was found in BAL of non-small-cell lung cancer patients than in COPD patients (P = 0.01). The calculated number of TfR molecules per cell in BAL correlated positively with the percentage of macrophages in BAL (P < 0.0001), suggesting that cell-associated TfR in BAL originates primarily from macrophages in this fluid. No correlation existed between BAL cell-associated TfR and tumour size, nodal status, the presence of metastases and serum sTfR. BAL cell-associated TfR was negatively correlated with BAL supernatant neuron-specific enolase (NSE) (P = 0.01). A combination of BAL supernatant NSE and cell-associated TfR detected lung cancer with a sensitivity of 91%, a specificity of 59% and positive and negative predictive values of 81% and 71% respectively. In conclusion, BAL cell-associated TfR may help in the differential diagnosis of lung cancer vs pneumonia.
Disciplines :
Hematology
Author, co-author :
Dowlati, A.
Loo
Bury, Thierry ;  Centre Hospitalier Universitaire de Liège - CHU > Pneumologie-Allergologie
Fillet, Georges ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
Beguin, Yves  ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
Language :
English
Title :
Soluble and cell-associated transferrin receptor in lung cancer.
Publication date :
1997
Journal title :
British Journal of Cancer
ISSN :
0007-0920
eISSN :
1532-1827
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
75
Issue :
12
Pages :
1802-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 February 2009

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