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In vitro stimulation of human gingival epithelial cell attachment to dentin by surface conditioning.
Van Heusden, Alain; Goffinet, Gerhard; Zahedi, Sharham et al.
1999In Journal of Periodontology, 70 (6), p. 594-603
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Keywords :
Anti-Bacterial Agents/pharmacology; Cell Adhesion/drug effects; Cells, Cultured; Citric Acid/pharmacology; Dentin/drug effects; Epithelial Attachment/cytology/physiology; Humans; Keratinocytes/physiology/ultrastructure; Microscopy, Electron, Scanning; Minocycline/pharmacology; Models, Biological; Tooth Root/drug effects
Abstract :
[en] BACKGROUND: Chemical root conditioning is widely used to improve the outcome of regenerative periodontal therapies by favoring the attachment of the regenerated periodontal structures. Although the effect of root conditioning on periodontal mesenchymal cells is well documented, very little is known about its potential effect on the re-formation of the junctional epithelium, a crucial event for the protection of the wound. The goal of the present study was to test in vitro the consequences of dentin conditioning with citric acid or minocycline on the attachment kinetics and morphology of human gingival keratinocytes (HGK). METHODS: The attachment kinetics of HGK to samples of powdered human dentin (particle size 44 to 76 microm) were examined by use of 3H-labeled cells. The morphology of attached epithelial cells was then determined by scanning electron microscopy (SEM). RESULTS: When the initial adhesion kinetics of cells on untreated dentin were tested, the percentage of attached HGK proved to be dependent on the number of plated cells and the time of incubation (from 0 to 12 hours). Conditioning the dentin by 3% citric acid or by minocycline-HCl (at 0.01, 0.1, or 2.5%) significantly increased (P <0.005) keratinocyte attachment beyond 6 hours, without notable differences between the 2 substances at any concentration. The attachment kinetics of HGK preincubated for 24 hours by 10 microg/ml minocyline-HCl on untreated dentin was found to be similar to that observed for non-preincubated cells. These results are in agreement with the SEM observations: indeed, the surface conditioning of dentin significantly modified the morphology of attached HGK, whereas the preincubation of these cells with minocyline-HCl did not. CONCLUSIONS: These results suggest that minocycline-HCl does not exert a direct effect on human gingival epithelial cells. In contrast, conditioning the dentin by citric acid or by minocycline stimulates the attachment of HGK, which could lead to a rapid periodontal healing by favoring the re-formation of a junctional epithelium.
Disciplines :
Dentistry & oral medicine
Author, co-author :
Van Heusden, Alain ;  Centre Hospitalier Universitaire de Liège - CHU > Institut de dentisterie - prothèse fixée
Goffinet, Gerhard ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Zahedi, Sharham 
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Lapière, Charles M.
Rompen, Eric ;  Centre Hospitalier Universitaire de Liège - CHU > Médecine dentaire
Language :
English
Title :
In vitro stimulation of human gingival epithelial cell attachment to dentin by surface conditioning.
Alternative titles :
[fr] Stimulation in vitro de l'attachement des cellules épithéliales gingivales humaines à la dentine par un conditionnement de surface
Publication date :
1999
Journal title :
Journal of Periodontology
ISSN :
0022-3492
Publisher :
Amer Acad Periodontology, Chicago, United States - Illinois
Volume :
70
Issue :
6
Pages :
594-603
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 January 2009

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