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Apoptosis in v-myc-transfected MSU-1.1 fibroblasts is induced by cell-matrix contact and differs from that of normal dermal fibroblasts.
Niland, S.; Cremer, A.; Herzhoff, K. et al.
2001In In Vitro Cellular and Developmental Biology. Animal, 37 (9), p. 606-12
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Keywords :
Animals; Apoptosis/physiology; Cells, Cultured; Collagen/physiology; Extracellular Matrix/physiology; Fibroblasts/cytology/physiology; Genes, myc; Humans; Kinetics; L Cells (Cell Line); Mice; RNA, Messenger/genetics; Recombinant Proteins/biosynthesis; Retroviridae Proteins, Oncogenic/genetics; Skin/cytology; Skin Physiological Phenomena; Transfection
Abstract :
[en] In order to characterize a fibroblast cell line representing normal human skin fibroblasts in three-dimensional cultures, we compared the fibroblast line MSU-1.1, derived from human foreskin and immortalized by v-myc, to primary human dermal fibroblasts (NDF). Our results demonstrate that in contrast to NDF, all MSU-1.1 fibroblasts die within 3-4 d when cultured within three-dimensional contractile collagen matrices. Also, in contrast to NDF. MSU-1.1 cells die markedly in anchored collagen gels as well. Death is due to apoptosis and is attenuated by addition of antibodies against collagen-recognizing receptors alpha1beta1 and alpha2beta1. Apoptosis of NDF in collagen lattices was repressed by an inhibitor of caspase-1, which was ineffective on apoptosis of MSU-1.1. Further, apoptosis by MSU-1.l fibroblasts was also observed in anchored, i.e., restrained collagen lattices, an environment that supports proliferation of NDF.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Niland, S.;  University of Cologne, Germany > Dept of Dermatology
Cremer, A.
Herzhoff, K.
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Lapiere, C. M.
Krieg, T.;  University of Cologne - Germany > Dept of Dermatology and Center for Biochemistry
Eckes, B.;  University of Cologne, Germany > Dept of Dermatology
Language :
English
Title :
Apoptosis in v-myc-transfected MSU-1.1 fibroblasts is induced by cell-matrix contact and differs from that of normal dermal fibroblasts.
Publication date :
2001
Journal title :
In Vitro Cellular and Developmental Biology. Animal
ISSN :
1071-2690
eISSN :
1543-706X
Publisher :
Society for in Vitro Biology, Columbia, United States - Maryland
Volume :
37
Issue :
9
Pages :
606-12
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 May 2010

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