[en] In order to characterize a fibroblast cell line representing normal human skin fibroblasts in three-dimensional cultures, we compared the fibroblast line MSU-1.1, derived from human foreskin and immortalized by v-myc, to primary human dermal fibroblasts (NDF). Our results demonstrate that in contrast to NDF, all MSU-1.1 fibroblasts die within 3-4 d when cultured within three-dimensional contractile collagen matrices. Also, in contrast to NDF. MSU-1.1 cells die markedly in anchored collagen gels as well. Death is due to apoptosis and is attenuated by addition of antibodies against collagen-recognizing receptors alpha1beta1 and alpha2beta1. Apoptosis of NDF in collagen lattices was repressed by an inhibitor of caspase-1, which was ineffective on apoptosis of MSU-1.1. Further, apoptosis by MSU-1.l fibroblasts was also observed in anchored, i.e., restrained collagen lattices, an environment that supports proliferation of NDF.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Niland, S.; University of Cologne, Germany > Dept of Dermatology
Cremer, A.
Herzhoff, K.
Nusgens, Betty ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Lapiere, C. M.
Krieg, T.; University of Cologne - Germany > Dept of Dermatology and Center for Biochemistry
Eckes, B.; University of Cologne, Germany > Dept of Dermatology
Language :
English
Title :
Apoptosis in v-myc-transfected MSU-1.1 fibroblasts is induced by cell-matrix contact and differs from that of normal dermal fibroblasts.
Publication date :
2001
Journal title :
In Vitro Cellular and Developmental Biology. Animal
ISSN :
1071-2690
eISSN :
1543-706X
Publisher :
Society for in Vitro Biology, Columbia, United States - Maryland
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