[en] PURPOSE: To investigate the incidence of translocations induced by iodine-131 therapy in thyrotoxicosis patients 1 year after the administration of the radiolabelled compound. MATERIALS AND METHODS: Tricolour FISH with whole-chromosome-specific probes for chromosomes 2, 4 and 8 was used for scoring translocations. From the genomic translocation frequencies, derived using the Lucas formula, equivalent whole-body doses were calculated, based on the in vitro (60)Co gamma-ray dose-response curve. RESULTS: A total of 101 translocations were observed in 4864 metaphases, 63% being of the two-way type. In the control group used for obtaining dose-response data, nine translocations were observed in 5278 metaphases, 55% being two-way translocations. No correlation was found between the observed frequency of translocations and administered radioactivity. Using the in vitro dose-response, an estimated average dose for the group of nine patients of 0.79 +/- 0.22Gy was obtained. Compared with frequencies following the assumption that the involvement of a particular chromosome in a two-break exchange-type aberration is proportional to its DNA content, chromosome 4 was more frequently involved and chromosomes 2 and 8 less frequently involved in chromosomal rearrangements. CONCLUSION: This study shows that (131)I therapy for thyrotoxicosis patients induced translocations, especially in chromosome 4, which could be detected 1 year after the administration of the radiolabelled compound.
Disciplines :
General & internal medicine
Author, co-author :
Lambert, Vincent ; Centre Hospitalier Universitaire de Liège - CHU > Ophtalmologie
Thierens, H.
Monsieurs, M.
Roncancio, C.
Laurent, C.
Language :
English
Title :
Translocation Frequencies Measured in Patients One Year after Radioactive Iodine Therapy for Thyrotoxicosis
Barquinero J.F., Knehr S., Braselmann H., Figel M., Bauchinger M. (1998) DNA-proportional distribution of radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization painting of all chromosomes of a human female karyotype. International Journal of Radiation Biology 74:315-323.
Baugnet-Mahieu L., Lemaire M., Leonard E., Leonard A., Gerber G. (1994) Chromosome aberrations after treatment with radioactive iodine for thyroid cancer. Radiation Research 140:429-431.
Boei J.J., Vermeulen S., Natarajan A.T. (1997) Differential involvement of chromosomes 1 and 4 in the formation of chromosomal aberrations in human lymphocytes after X-irradiation. International Journal of Radiation Biology 72:139-145.
Catena C., Villani P., Nastasi R., Conti D., Righi E., Salerno G., Righi V.A., Ronga G. (1994) Micronuclei and 3AB-index in patients receiving iodine-131 therapy. Journal of Nuclear Biology and Medicine 38:586-593.
Cigarran S., Barrios L., Barquinero J.F., Caballin M.R., Ribas M., Egozcue J. (1998) Relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural aberrations, analysed by painting. International Journal of Radiation Biology 74:449-455.
Clarke S. (1991) Radionuclide therapy of the thyroid. European Journal of Nuclear Medicine 18:984-991.
De Vathaire F., Schlumberger M., Delisle M., Francese C., Challeton C., De la Genardiere E., Meunier F., Parmentier C., Hill C., Sancho-Garnier H. (1997) Leukaemias and cancers following iodine-131 administration for thyroid cancer. British Journal of Cancer 75:734-739.
Dottorini M., Lomuscio G., Mazzucchelli L., Vignati A., Colombo L. (1995) Assessment of female fertility and carcinogenesis after iodine-131 therapy for differentiated thyroid carcinoma. Journal of Nuclear Medicine 36:21-27.
Edmonds C., Smith T. (1986) The long-term hazards of the treatment of thyroid cancer with radioiodine. British Journal of Radiology 59:45-51.
Finnon P., Moquet J.E., Edwards A.A., Lloyd D.C. (1999) The 60Co gamma ray dose-response for chromosomal aberrations in human lymphocytes analysed by FISH; applicability to biological dosimetry. International Journal of Radiation Biology 75:1215-1222.
Gebhart E., Neubauer S., Schmitt G., Birkenhake S., Dunst J. (1996) Use of a three-color chromosome in situ suppression technique for the detection of past radiation exposure. Radiation Research 145:47-52.
Gobbi A., Di Berardino C., Scanziani E., Garofalo A., Rivolta A., Fontana G., Rambaldi A., Giavazzi R., Biondi A. (1997) A human acute lymphoblastic leukemia line with the T(4;11) translocation as a model of minimal residual disease in SCID mice. Leukemia Research 21:1107-1114.
Gutierrez S., Carbonell E., Galofre P., Creus A., Marcos R. (1997) Micronuclei induction by 1311 exposure: Study in hyperthyroidism patients. Mutation Research 373:39-45.
Hall P., Berg G., Bjelkengren G., Boice J.D. Jr., Ericsson U.B., Hallquist A., Lidberg M., Lundell G., Tennvall J., Wiklund K. (1992) Cancer mortality after iodine-131 therapy for hyperthyroidism. International Journal of Cancer 50:886-890.
Halnan K.E. (1992) Leukaemia after iodine-131 exposure. Lancet 340:437.
Hertz S., Roberts A. (1942) Radioactive iodine as an indicator in thyroid physiology: Use of radioactive iodine in differential diagnosis of 2 types of Grave's disease. Journal of Clinical Investigation 21:31.
Holm L. (1991) Radiation-induced thyroid neoplasia. Sozial-und Praventivmedizin 36:266-275.
Kapelushnik J., Dube I., Wilson P., Greenberg M. (1991) Acute lymphoblastic leukemia with t(4;11) translocation after osteogenic sarcoma. Cancer 67:2887-2889.
Knehr S., Zitzelsberger H., Braselmann H., Nahrstedt U., Bauchinger M. (1996) Chromosome analysis by fluorescence in situ hybridization: Further indications for a non-DNA-proportional involvement of single chromosomes in radiation-induced structural aberrations. International Journal of Radiation Biology 70:385-392.
Lindholm C., Loumahaara S., Koivistoinen A., Ilus T., Edwards A.A., Salomaa S. (1998) Comparison of dose-response curves for chromosomal aberrations established by chromosome painting and conventional analysis. International Journal of Radiation Biology 74:27-34.
Lindholm C., Tekkel M., Veidebaum T., Ilus T., Salomaa S. (1998) Persistence of translocations after accidental exposure to ionizing radiation. International Journal of Radiation Biology 74:565-571.
Livingston G.K., Foster A.E., Elson H.R. (1993) Effect of in vivo exposure to iodine-131 on the frequency and persistence of micronuclei in human lymphocytes. Journal of Toxicology and Environmental Health 40:367-375.
Lucas J.N., Deng W., Moore D., Hill F., Wade M., Lewis A., Sailes F., Kramer C., Hsieh A., Galvan N. (1999) Background ionizing radiation plays a minor role in the production of chromosome translocations in a control population. International Journal of Radiation Biology 75:819-827.
Lucas J.N., Hill F.S., Burk C.E., Cox A.B., Straume T. (1996) Stability of the translocation frequency following whole-body irradiation measured in rhesus monkeys. International Journal of Radiation Biology 70:309-318.
Luomahaara S., Lindholm C., Mustonen R., Salomaa S. (1999) Distribution of radiation-induced exchange aberrations in human chromosomes 1, 2 and 4. International Journal of Radiation Biology 75:1551-1556.
M'Kacher R., Legal J.D., Schlumberger M., Voisin P., Aubert B., Gaillard N., Parmentier C. (1996) Biological dosimetry in patients treated with iodine-131 for differentiated thyroid carcinoma. Journal of Nuclear Medicine 37:1860-1864.
M'Kacher R., Schlumberger M., Legal J.D., Violot D., Beron-Gaillard N., Gaussen A., Parmentier C. (1998) Biologic dosimetry in thyroid cancer patients after repeated treatments with iodine-131. Journal of Nuclear Medicine 39:825-829.
Matsuoka A., Tucker J.D., Hayashi M., Yamazaki N., Sofuni T. (1994) Chromosome painting analysis of X-ray-induced aberrations in human lymphocytes in vitro. Mutagenesis 9:151-155.
Mazzaferri E.L., Jhiang S.M. (1994) Long-term impact of initial surgical and medical therapy on papillary and follicular thyroid cancer. American Journal of Medicine , erratum, 1995, 98, 215; 97:418-428.
Monsieurs M., Thierens H., Wiele C., Vral A., Meirlaen I., De Winter H., De Sadeleer C., De Ridder L., Kaufman J., Dierckx R. (1999) Estimation of risk based on biological dosimetry for patients treated with radioiodine. Nuclear Medicine Communication 20:911-917.
Morton N.E. (1991) Parameters of the human genome. Proceedings of the National Academy of Sciences, USA 88:7474-7476.
Natarajan A.T., Vyas R.C., Darroudi F., Vermeulen S. (1992) Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by in situ hybridization using chromosome-specific DNA libraries. International Journal of Radiation Biology 61:199-203.
Palau F., Prieto F., Badia L., Beneyto M., Sempere A., Borrego D. (1991) Cytogenetic evidence of involvement of an early progenitor myeloid cell in 4;11 translocation-associated acute leukemia. Cancer Genetics and Cytogenetics 51:79-84.
Pathak S., Staroselsky A.N., Fidler I.J. (1991) Specific chromosomal defects associated with metastatic potential in K-1735 melanoma clones. Involvement of chromosomes 4 and 14. Cancer Genetics Cytogenetics 56:209-221.
Ramirez M.J., Surralles J., Galofre P., Creus A., Marcos R. (1997) Radioactive iodine induces clastogenic and age-dependent aneugenic effects in lymphocytes of thyroid cancer patients as revealed by interphase FISH. Mutagenesis 12:449-455.
Reichel M., Gillbert E., Nilson I., Siegler G., Greil J., Fey G.H., Marschalek R. (1998) Fine structure of translocation breakpoints in leukemic blasts with chromosomal translocation t(4;11): The DNA damage-repair model of translocation. Oncogene 17:3035-3044.
Snigiryova G., Braselmann H., Salassidis K., Shevchenko V., Bauchinger M. (1997) Retrospective biodosimetry of Chernobyl clean-up workers using chromosome painting and conventional chromosome analysis. International Journal of Radiation Biology 71:119-127.
Stephan G., Pressl S. (1997) Chromosome aberrations in human lymphocytes analysed by fluorescence in situ hybridization after in vitro irradiation, and in radiation workers, 11 years after an accidental radiation exposure. International Journal of Radiation Biology 71:293-299.
Thierens H., Vral A., De Ridder L., Touil N., Kirsch-Volders M., Lambert V., Laurent C. (1999) Inter-laboratory comparison of cytogenetic endpoints for the biomonitoring of radiological workers. International Journal of Radiation Biology 75:23-34.
Tucker J.D., Ramsey M.J., Lee D.A., Minkler J.L. (1993) Validation of chromosome painting as a biodosimeter in human peripheral lymphocytes following acute exposure to ionizing radiation in vitro. International Journal of Radiation Biology 64:27-37.
Watanabe N., Yokoyama K., Kinuya S., Shuke N., Shimizu M., Futatsuya R., Michigishi T., Tonami N., Seto H., Goodwin D.A. (1998) Radiotoxicity after iodine-131 therapy for thyroid cancer using the micronucleus assay. Journal of Nuclear Medicine 39:436-440.
Wuttke K., Streffer C., Muller W.U., Reiners C., Biko J., Demidchik E. (1996) Micronuclei in lymphocytes of children from the vicinity of Chernobyl before and after 131I therapy for thyroid cancer. International Journal of Radiation Biology 69:259-268.
Zanzonico P.B. (1997) Radiation dose to patients and relatives incident to 131I therapy. Thyroid 7:199-204.
