[en] Monoaminergic neurons have been shown to play a role in both the intoxicating and chronic effects of ethanol. We present here the results of a study about the acute effects of ethanol on serotonergic raphe nucleus, noradrenergic locus coeruleus, and dopaminergic ventral tegmental area. These nuclei were investigated electrophysiologically by recording the spontaneous firing rate of single neurons using glass microelectrodes, both in vivo in chloral hydrate anesthetized rats and in vitro in brain slices. Ethanol was perfused intravenously at a rate ranging from 0.2 mg/kg/min to 0.2 g/kg/min in vivo, and at concentrations between 10(-8) M and 1 M in vitro. We observed that each monoaminergic nucleus had its own pattern of responses to acute ethanol perfusion, and that high and low concentrations have different actions, suggesting a biphasic effect. For example, in slices, ethanol concentrations higher than 10 mM induce an excitation in most raphe and ventral tegmental area neurons, and an inhibition of firing in locus coeruleus neurons. The results were comparable in the in vivo model, but much more heterogenous. We conclude that the effect of ethanol on the monoaminergic neurons is specific of the type of neuron, and that a biphasic effect is commonly found.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Verbanck, P.
Seutin, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie
Dresse, Albert ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Scuvée-Moreau, Jacqueline ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie - Département des sciences biomédicales et précliniques
Massotte, Laurent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Giesbers, Irène ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie
Kornreich, C.
Language :
English
Title :
Electrophysiological effects of ethanol on monoaminergic neurons: an in vivo and in vitro study.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Ollat H, Parvez H, Parvez S (1988) Alcohol and central neurotransmission. Neurochem Int 13(3):257-300.
Fibiger HC, Le Piane FG, Jakubovic A, Phillips G (1986) The role of dopamine in intracranial self‐stimulation of the ventral tegmental area. J Neurosci 7(12):3888-3896.
Phillips AG, Fibiger HC (1978) The role of dopamine in maintaining intracranial self‐stimulation in the ventral tegmentum, nucleus accumbens and medial prefrontal cortex. Can J Physiol Pharmacol 32:58-66.
Kornetsky C, Bain GT, Unterwald EM, Lewis MJ (1988) Brain stimulation reward: Effects of ethanol. Alcohol Clin Exp Res 12:609-616.
Di Chiara G, Imperato A (1988) Drugs abused by humans preferentially increase synaptic dopamine concentration in the mesolimbic system of freely moving rats. Proc Natl Acad Sci USA 85:5274-5278.
Myers RD, Melchior GL (1975) Alcohol drinking in the rat after destrution of serotoninergic and catecholaminergic neurons in the brain. Res Commun Chem Pathol Pharmacol 10:363-377.
Borg S, Kvande H, Mossberg D, Valverius P, Sedvall G (1983) Central nervous system noradrenaline metabolism and alcohol consumption in man. Pharmacol Biochem Behav 18:375-378.
Hawley RJ, Major LF, Schulman CA, Linnoila M (1985) Cerebrospinal fluid 3‐methoxy‐4‐hydroxyphenylglycol and norepinephrine levels in alcohol withdrawal. Arch Gen Psychiatry 42:1056-1062.
Linnoila M, Mefford J, Nutt D, Adinoff B (1987) Alcohol withdrawal and noradrenergic function. Ann Intern Med 107:875-889.
Baumgartner GR, Rowen RC (1987) Clonidine versus chlordiazepoxide in the management of acute alcohol withdrawal syndrome. Arch Intern Med 147:1223-1226.
Hill SY (1974) Intraventricular injection of 5‐hydroxytryptamine and alcohol consumption in rats. Biol Psychiatry 8:151-158.
Geller I (1973) Effects of para‐chlorphenylalanine and 5‐hydroxytryptophan on alcohol intake in the rat. Biol Biochem Behav 1:361-365.
Myers MD, Martin GE (1973) The role of cerebral serotonin in the ethanol preference of animals. Ann NY Acad Sci 215:135-144.
Amit Z, Sutherland EA, Gill K, Ogren S (1984) Zimelidine: A review of its effects on ethanol consumption. Neurosci Biobehav Rev 8:35-54.
Naranjo CA, Sellers EM, Roach CA, Woodley DV, Sanchez‐Craig M, Sykora K (1984) Zimelidine‐induced variations in alcohol intake by non‐depressed heavy drinkers. Clin Pharmacol Ther 35:374-381.
Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates, Sidney, Academic Press; 1982.
Haas HL, Schaerer B, Vosmansky M (1979) A simple perfusion chamber for the study of nervous tissue slices in vitro. J Neurosci Meth 1:323-325.
Baraban JM, Aghajanian GK (1983) Suppression of firing activity of 5HT neurons in the dorsal raphe by alpha‐adrenoreceptors antagonists. Neuropharmacology 19:355-363.
Vandermaelen CR, Aghajanian GK (1983) Electrophysiological and pharmacological characterization of serotonergic dorsal raphe neurons recorded extracellularly in rat brain slices. Brain Res 289:109-119.
Svensson TH, Bunney BS, Aghajanian GK (1975) Inhibition of both noradrenergic and serotonergic neurons in brain by the alpha‐adrenergic agonist clonidine. Brain Res 92:291-306.
Bunney BS, Aghajanian GK, Roth RH (1973) 1‐DOPA, amphetamine and apomorphine comparison of effects on the firing rate of rat dopaminergic neurons. Nature 245:123.
Aghajanian GK, Haigler HJ, Bloom FE (1972) Lysergic acid diethylamine and serotonin: Direct action on serotonin‐containing neurons in rat brain. Life Sci 11:615-622.
Gessa GL, Muntoni F, Collu M, Vargiu L, Mereu G (1985) Low doses of ethanol activate dopaminergic neurons in the ventral tegmental area. Brain Res 348(1):201-203.
Goldstein DB Pharmacology of Ethanol, New York, Oxford University Press; 1983.
Majchrowicz E (1975) Induction of physical dependence upon ethanol and the associated behavioral changes. Psychopharmacologia (Berlin) 43:245-254.
Berild D, Hasselbach H (1981) Survival after a blood alcohol of 1127 mg/dl. Lancet 2:363.
Johnson RA, Noll EC, Rodney WM (1982) Survival after a serum ethanol concentration of 1,5%. Lancet 2:1394.
Watanabe A, Kobayashi M, Hobara N, Nakatsukasa H, Nagashima H, Fujimoto A (1985) A report of unusually high blood ethanol and acetaldehyde levels in two surviving patients. Alcohol Clin Exp Res 9:14-16.
Schoener EP (1984) Ethanol effect on striatal neuron activity. Alcohol Clin Exp Res 8:266-268.
Grupp LA (1980) Biphasic action of ethanol on single units of the dorsal hippocampus and the relationship to the cortical EEG. Psychopharmacology 70:95-103.
Mereu G, Gessa GK (1985) Low doses of ethanol inhibit the firing of neurons in the substantia nigra, pars reticulata: A GABAergic effect. Brain Res 360:325-330.
Tabakoff B, Hoffman PL (1983) Neurochemical aspects of tolerance to and physical dependence on alcohol. The Biology of Alcoholism , Kissin B, Begleiter H, (eds):,. New York, Plenum Press; 7:199.
Bacopoulos NG, Bhatanger RK, van Orden LS (1978) The effects of subhypnotic doses of ethanol on regional catecholamines turnover. J Pharmacol Exp Ther 204:1-10.
Hunt WA, Majchrowicz E (1974) Alterations in the turnover of brain norepinephrine and dopamine in the alcohol‐dependent rat. J Neurochem 23:549-552.
Pohorecky LA, Brick J (1977) Activity of neurons in the locus coeruleus of the rat: Inhibition by ethanol. Brain Res 131:171-179.
Hunt WA, Majchrowicz E (1974) Alterations in the turnover of brain norepinephrine and dopamine in the alcohol‐dependent rat. J Neurochem 23:549-552.
Strahlendorf JC, Strahlendorf HK (1982) Response of locus coeruleus neurons to direct application of ethanol. Neuroscience 7:312.
Swanson LW (1976) The locus coeruleus: A cytoarchitectonic, Golgi and immunohistochemical study in the Albino rat. Brain Res 110:39-56.
Grzanna R, Molliver ME (1980) The locus coeruleus in the rat: An immunohistochemical delineation. Neuroscience 5:21-40.
Suzdak PD, Schwartz RD, Skolnick P, Paul SM (1986) Ethanol stimulates gamma‐aminobutyric acid‐receptor mediated choride transport in rat brain synaptoneurosomes. Proc Natl Acad Sci USA 83:4071-4075.
Hyatt MC, Tyce GM (1985) The effects of ethanol on the efflux and release of norepinephrine and 5‐hydroxytryptamine from slices of rat hypothalamus. Brain Res 337:255-262.
Chu NS, Keenan L (1987) Responses of midbrain dorsal raphe neurons to ethanol studied in brainstem slices. Alcohol 4(5):373-374.
Charness ME, Gordon AS, Diamond I (1983) Ethanol modulation of opiate receptors in cultured neural cells. Science 222:1246-1248.
Lovinger DM, White G, Weight F (1989) Ethanol inhibits NMDA‐activated ion current in hippocampal neurons. Science 243:1721-1724.
Verbanck P, Seutin V, Dresse A (1989) RO‐1788 and picrotoxin inhibit the effects of high but not of low ethanol concentrations on the rat locus coeruleus in vitro. Alcohol Alcohol 24(4):389.
Verbanck P, Seutin V, Dresse A (1989) Yohimbine can induce ethanol tolerance in an in vitro preparation of rat locus coeruleus. Alcohol Alcohol 24(4):390.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.