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Assessment of Dynein-Mediated Nuclear Migration in the Developing Cortex by Live-Tissue Microscopy
Cheng, Haw-Yuan; Nian, Fang-Shin; Ou, Y.-W. et al.
2023In Methods in Molecular Biology
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Keywords :
Brain slice culture; Cortical development; In utero electroporation; Interkinetic nuclear migration; Live-tissue imaging; Neural stem cell; Radial glial cell; Radial migration; Cell Movement; Cerebral Cortex; Dyneins; Electroporation; Microscopy; Neural Stem Cells; Neurons; dynein adenosine triphosphatase; animal cell; animal experiment; animal tissue; brain cortex; brain development; brain slice; cell cycle progression; cell migration; cell nucleus; cell structure; controlled study; electroporation; epifluorescence microscopy; female; live cell imaging; macroglia; microscopy; mitosis; mouse; neural stem cell; nonhuman; cell motion; genetics; metabolism; nerve cell; physiology; procedures
Abstract :
[en] During development of the cerebral cortex, neuroepithelial and radial glial cells undergo an oscillatory nuclear movement throughout their cell cycle, termed interkinetic nuclear migration. The nucleus of postmitotic neurons derived from these neural stem cells also translocates in a saltatory manner to enable neuronal migration toward the cortical plate. In these processes, various molecular motors, including cytoplasmic dynein, myosin II, and kinesins, are the driving force for nuclear migration at different stages. Despite efforts made to understand the mechanism regulating cortical development over decades, novel gene mutations discovered in neurodevelopmental disorders indicate that missing pieces still remain. Gene manipulation by in utero electroporation combined with live microscopy of neural stem cells in brain slices provides a powerful method to capture their detailed behaviors during proliferation and migration. The procedures described in this chapter enable the monitoring of cell cycle progression, mitosis, morphological changes, and migratory patterns in situ. This approach facilitates the elucidation of gene functions in cortical development and neurodevelopmental disorders. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Disciplines :
Biotechnology
Author, co-author :
Cheng, Haw-Yuan  ;  Université de Liège - ULiège > GIGA > GIGA Neurosciences - Molecular Regulation of Neurogenesis ; Institute of Brain Science, College of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan
Nian, Fang-Shin ;  Université de Liège - ULiège > GIGA > GIGA Neurosciences - Molecular Regulation of Neurogenesis ; Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan ; Institute of Brain Science, College of Medicine, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan
Ou, Y.-W.;  Brain Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan
Tsai, J.-W.;  Department of Biological Science & Technology, College of Biological Science and Technology, Hsinchu, Taiwan
Language :
English
Title :
Assessment of Dynein-Mediated Nuclear Migration in the Developing Cortex by Live-Tissue Microscopy
Publication date :
2023
Main work title :
Methods in Molecular Biology
Publisher :
Humana Press Inc.
Peer reviewed :
Editorial reviewed
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