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Use of human AML cells to study graft-versus-leukemia immunity in xenogeneic mouse models of GVHD
Faville, Charline; E Silva, Bianca; Baron, Frédéric et al.
2024
 

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Abstract :
[en] AbstractAllogeneic hematopoietic cell transplantation (allo-HCT) is the main therapeutic approach for patients with high-risk acute myeloid leukemia (AML), but the rate of relapse remains high and is associated with poor outcomes. Discovering new approaches to maximize the graft-versus-leukemia (GVL) effects while mitigating graft-versus-host disease (GVHD) should therefore be pursued. Because of the difficulties in modeling AML in mice, patient-derived xenotransplantations (PDX) in immunodeficient NSG mice are preferred to study the GVL effects. In PDX, AML is typically induced through the intravenous injection of cell lines or leukemic blasts obtained from patients. GVHD and GVL effects are induced by (co)-injecting human T cells or peripheral blood mononuclear cells (PBMCs). While this approach enables the induction of systemic leukemia, notably developing in the spleen and bone marrow of the animals, it can also be associated with difficulties in monitoring the disease, notably by flow cytometry. This can be circumvented by using luciferase-expressing AML cells or transplanting the leukemic cells in Matrigel to generate solid tumors that are easier to monitor. Here, we provide detailed instructions on how to prepare human PBMCs and leukemic cells, transplant them, and monitor the disease in NSG mice.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Faville, Charline  ;  Université de Liège - ULiège > GIGA
E Silva, Bianca  ;  Université de Liège - ULiège > GIGA
Baron, Frédéric  ;  Université de Liège - ULiège > Département des sciences cliniques
Ehx, Grégory  ;  Université de Liège - ULiège > Département des sciences cliniques
Language :
English
Title :
Use of human AML cells to study graft-versus-leukemia immunity in xenogeneic mouse models of GVHD
Publication date :
02 May 2024
Source :
Available on ORBi :
since 03 July 2024

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