Mitochondrial Genome Transmission in Chlamydomonas Diploids Obtained by Sexual Crosses and Artificial Fusions: Role of the Mating Type and of a 1 Kb Intron
Remacle, Claire; Bovie, C.; Michel-Wolwertz, M. R.et al.
1990 • In Molecular and General Genetics, 223 (2), p. 180-4
[en] The linear mitochondrial DNAs of the two infertile algal species Chlamydomonas smithii and C. reinhardtii are co-linear with the exception of a 1 kb intron (alpha intron) located in the cytochrome b gene of C. smithii. C. smithii also possesses an additional HpaI restriction site (H marker) located in the COXI gene, about 5 kb from the intron. In reciprocal crosses, C. smithii (H+ alpha +) x C. reinhardtii (H- alpha -), the alpha intron is transmitted to all diploid progeny, whereas the H marker is frequently transmitted either biparentally or paternally depending on whether the C. smithii parent is maternal (mt+) or paternal (mt-). In diploids resulting from artificial fusion between vegetative cells, the absolute transmission of alpha is accompanied by the frequent transmission of the H+ marker, irrespective of the mating type of the parental strains. Finally, in reciprocal crosses between C. smithii (H+ alpha +) and recombinant H- alpha + clones, the transmission of the H marker is predominantly paternal or biparental. These results allow us to conclude that (1) the alpha intron behaves as a group I intron whose unidirectional conversion influences the transmission of the H marker; and (2) the mt- paternal mitochondrial genome is transmitted more often than the mt+. The mating type has no effect in diploids obtained by artificial fusion.
Remacle, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Génétique
Bovie, C.
Michel-Wolwertz, M. R.
Loppes, Roland ; Université de Liège - ULiège > Génétique des microorganismes
Matagne, René-Fernand ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Mitochondrial Genome Transmission in Chlamydomonas Diploids Obtained by Sexual Crosses and Artificial Fusions: Role of the Mating Type and of a 1 Kb Intron
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