Ultrastructural nucleolar alterations induced by an ametantrone/polyr(A-U) complex.

Thiry, Marc; Jamison, J. M.; Gilloteaux, J.; Summers, J. L.; Goessens, Guy

doi:10.1006/excr.1997.3743

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Ultrastructural nucleolar alterations induced by an ametantrone/polyr(A-U) complex.

Thiry, Marc; Jamison, J. M.; Gilloteaux, J. et al.

1997 • In Experimental Cell Research, 236 (1), p. 275-84

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https://hdl.handle.net/2268/29323

DOI
10.1006/excr.1997.3743

PubMed
9344608

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Keywords :

Antineoplastic Agents/toxicity; Cell Nucleolus/chemistry/drug effects/ultrastructure; DNA, Neoplasm/analysis; Dose-Response Relationship, Drug; Humans; Immunohistochemistry; Interferon Inducers/toxicity; Microscopy, Electron; Mitoxantrone/analogs & derivatives/toxicity; Nucleic Acids/analysis; Poly A-U/toxicity; RNA, Neoplasm/analysis; Tumor Cells, Cultured/drug effects/ultrastructure; Urinary Bladder Neoplasms

Abstract :

[en] In the present study we examined the ultrastructural modifications as well as the precise distribution of DNA and RNA in RT4 cell nucleoli following a 3-h exposure to nontoxic or toxic doses of ametantrone (AMT), poly(adenylate-uridylate) (polyr(A-U), or an AMT/polyr(A-U) combination. While distribution of nucleic acids within the various nucleolar components is not modified following all treatments, the nucleoli exhibit several ultrastructural changes: redistribution of the nucleolar components, decrease in the number of fibrillar centers, and increase in the size of the fibrillar centers. The relative frequencies of the test agents to induce the apparition of nucleoli of compact type are AMT/polyr(A-U) > AMT approximately polyr(A-U) > sham-treated, while the abilities of the test agents to induce the nucleolar segregation are AMT/polyr(A-U) approximately AMT > polyr(A-U) > sham-treated cells. These ultrastructural changes are characteristics of drugs that intercalate into DNA and inhibit rDNA transcription as well as rRNA processing and release of nascent preribosomes from the nucleolus.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire

Jamison, J. M.

Gilloteaux, J.

Summers, J. L.

Goessens, Guy ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Language :

English

Title :

Ultrastructural nucleolar alterations induced by an ametantrone/polyr(A-U) complex.

Publication date :

1997

Journal title :

Experimental Cell Research

ISSN :

0014-4827

eISSN :

1090-2422

Publisher :

Academic Press, Orlando, United States - Florida

Volume :

236

Issue :

Pages :

275-84

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 November 2009

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