[en] A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Bendayan (1984) Enzyme-gold electron microscopic cytochemistry: a new affinity approach for the ultrastructural localization of macromolecules. J. Electron. Microscopy Technique 1:349-372.
Ellwart, Dormer (1985) Effect of 5-fluoro-2′-deoxyuridine (FdUrd) on 5-bromo-2′-deoxyuridine (BrdUrd) incorporation into DNA measured with a monoclonal BrdUrd antibody and by the BrdUrd/Hoechst quenching effect. Cytometry 6:513-520.
Fakan (1978) High resolution autoradiography studies on chromatin functions. The Cell Nucleus , H. Busch, Academic Press, New York; 5:3-53.
Fakan, Modak (1973) Localization of DNA in ultrathin tissue sections incubated with terminal deoxynucleotidyl transgerase as visualized by electronmicroscope autoradiography. Exp. Cell. Res. 77:95-104.
Gautier (1976) Ultrastructural localization of DNA in ultrathin tissue sections. Int. Rev. Cyt. 44:113-191.
Goz (1978) The effects of incorporation of 5-halogenated deoxyuridines into DNA of eukaryotic cells. Pharmacol. Rev. 29:249-272.
Gratzner (1982) Monoclonal antibody to 5-bromo and 5-iodo-deoxyuridine: a new reagent for detection of DNA replication. Science 218:474-475.
Hansmann, Falk, Scheer, Sitte (1986) Ultrastructural localization of DNA in two cryptomonas species by use of a monoclonal DNA antibody. Eur. J. Cell Biol. 42:152-160.
Harms, Van Goor, Koudstaal, de Ley, Hardonk (1986) Immunohistochemical demonstration of DNA incorporated 5-bromodeoxyuridine in frozen and plastic embedded sections. Histochemistry 85:139-143.
Lepoint (1977) Selection of Ehrlich tumor cells in different phases of the cell cycle by ficoll gradient centrifugation. Virchows Arch. B. Cell Path. 25:53-60.
Moran, Darzynkiewicz, Staianocoico, Melamed (1985) Detection of 5-bromodeoxyuridine (BrdUrd) incorporation by monoclonal antibodies: Role of the DNA denaturation step. J. Histochem. Cytochem. 33:821-827.
Moyne (1980) Methods in ultrastructural cytochemistry of the cell nucleus. Progress in Histochem. and Cytochem. 13:1-70.
Raska, Armbruster, Jira, Hana, Kaslik, Rovensky, Smetana (1983) Ultrastructural localization of DNA in tissue culture cell nuclei by means of enzyme-gold and autoimmune sera-protein A-gold techniques. Biol. Cell 48:211-214.
Roth, Bendayan, Carlemalm, Villiger, Garavito (1981) Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J. Histochem. Cytochem. 29:663-671.
Scheer, Messner, Hazan, Raska, Hansmann, Falk, Spiess, Franke (1987) High sensitivity immunolocalization of double-and single-stranded DNA by a monoclonal antibody. Eur. J. Cell Biol. 43:358-371.
Simard (1967) The binding of actinomycin D-3H to heterochromatin as studied by quantitative high resolution radioautorgraphy. J. Cell Biol. 35:716-722.
Steinert, Van Gansen (1971) Binding of 3H-actinomycin to vitelline platelets of amphibian oocytes. A high resolution autoradiographic investigation. Exp. Cell Res. 64:355-365.
Sugihara, Hattori, Fukuda (1986) Immunohistochemical detection of bromodeoxyuridine in formalin-fixed tissues. Histochemistry 85:193-195.
Suzuki, Tsutsui, Takahashi (1974) Ultrastructural detection of single-stranded DNA in rat liver nucleus by the electronmicroscopic immuno-peroxidase method. Exp. Cell Res. 89:306-310.
Szybalski (1974) X-ray sensitization by halopyrimidines. Cancer Chemother. Rep. 58:539-557.
Thiry, Scheer, Goessens (1988) Localization of DNA within Ehrlich tumour cell nucleoli by immunoelectron microscopy. Eur. J. Cell Biology, submitted; .
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
