Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.

Thiry, Marc; Dombrowicz, D.

doi:10.1111/j.1768-322X.1988.tb00710.x

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Article (Scientific journals)

Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.

Thiry, Marc; Dombrowicz, D.

1988 • In Biology of the Cell, 62 (1), p. 99-102

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https://hdl.handle.net/2268/29267

DOI
10.1111/j.1768-322X.1988.tb00710.x

PubMed
2966644

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Keywords :

Animals; Antibodies, Monoclonal/diagnostic use; Bromodeoxyuridine/immunology; Carcinoma, Ehrlich Tumor; DNA/analysis/ultrastructure; DNA Replication; Floxuridine; Immunohistochemistry; Microscopy, Electron; Tumor Cells, Cultured

Abstract :

[en] A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire

Dombrowicz, D.

Language :

English

Title :

Anti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.

Publication date :

1988

Journal title :

Biology of the Cell

ISSN :

0248-4900

eISSN :

1768-322X

Publisher :

Portland Press, Colchester, United Kingdom

Volume :

Issue :

Pages :

99-102

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 November 2009

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Bibliography

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