[en] Basic fibroblast growth factor (bFGF), a growth factor for many cell types including newborn rat astroglial cells, stimulates in a dose-dependent fashion the release of plasminogen activators (PAs) by these cells as measured by the fibrin-overlay method or the Coleman and Green's colorimetric assay. This effect of bFGF on PAs secretion (about 4.5-fold increase at 40 ng/ml bFGF) does not result from an aspecific stimulation of protein secretion by astrocytes and is only partly correlated with the mitogenic activity of bFGF. bFGF was also tested on two clonal glioma cell lines (C6 and LN18). Only one of those cell types (LN18) showed a stimulated PA release in the presence of bFGF. These data are discussed with respect to the putative roles of plasminogen activators in the developing nervous system.
Rogister, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Leprince, Pierre ; Université de Liège - ULiège > GIGA - Neurosciences
Pettmann, B.
Labourdette
Sensenbrenner, M.
Moonen, Gustave ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Language :
English
Title :
Brain basic fibroblast growth factor stimulates the release of plasminogen activators by newborn rat cultured astroglial cells
Benda, Lightbody, Sato, Levine, Sweet (1968) Differentiated rat glial cell strain in tissue culture. Science 161:370-371.
Besnard, Perraud, Sensenbrenner, Labourdette (1987) Platelet-derived growth factor is a mitogen for glial but not for neuronal rat brain cells in vitro. Neurosci. Lett. 73:287-292.
Booher, Sensenbrenner (1972) Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask culture. Neurobiology 2:97-105.
Brockes (1984) Mitogenic growth factors and nerve dependence of limb regeneration. Science 225:1280-1287.
Coleman, Green (1980) A coupled photometric assay for plasminogen activator. Methods Enzymol. 80:408-414.
Diserens, De Tribolet, Martin-Achard, Gailde, Schnegg, Carrel (1981) Characterization of an established human malignant glioma cell line: LN 18. Acta Neuropathol. 53:21-27.
Fontana, Dubs, Merchant, Balsiger, Grob (1981) Glial-cell stimulating factor (GSF): a new lymphokine. Part 1: cellular sources and partial purification of murine GSF, role of cytoskeleton and protein synthesis in its production. J. Neuroimmunol. 2:55-71.
Gospodarowicz, Cheng, Lui, Baird, Bohlent (1984) Isolation of brain fibroblast growth factor by heparine Sepharose affinity chromatography: identity with pituitary fibroblast growth factor. Proc. Natl. Acad. Sci. U.S.A. , 7th edn.; 81:6963-6967.
Guenther, Nick, Monard (1985) A glia-derived neurite promoting factor with protease inhibitory activity. EMBO J. 4:1963-1966.
Heldin, Westermark, Wasteson (1979) Platelet-derived growth factor: purification and partial characterization. Proc. Natl. Acad. Sci. U.S.A. , 7th edn.; 76:3722-3726.
Laemmli (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature (Lond.) 227:680-685.
Leprince, Rogister, Moonen (1986) Identification and distribution of plasminogen activators and plasminogen-activator inhibitors in the mammalian nervous system. Arch. Int. Physiol. Biochem. 94:145.
Lim, Miller (1984) An improved procedure for the isolation of the Glia Maturation Factor. J. Cell Physiol. 119:255-259.
Lindner, Guenther, Nick, Zinser, Antonicek, Schachner, Monard (1986) Modulation of granule cells migration by a glia derived protein. Proceedings of the National Academy of Sciences , 7th edn.; 83:568-571.
Mira-y-Lopez, Joseph-Silverstein, Rifkin, Ossowski (1986) Identification of a pituitary factor responsible for enhancement of plasminogen activator activity in breast tumor cells. Proceedings of the National Academy of Sciences , 7th edn.; 83:7780-7784.
Moonen, Grau-Wagemans, Selak (1982) Plasminogen activators-plasmin system and neuronal migration. Nature (Lond.) 298:753-755.
Moonen, Grau-Wagemans, Selak, Lefebvre, Rogister, Vassali, Belin (1985) Plasminogen activator is a mitogen for astrocytes in developing cerebellum. Dev. Brain Res. 20:41-48.
Pettmann, Labourdette, Weibel, Sensenbrenner (1986) The brain fibroblasts growth factor FGF is localized in neurons. Neurosci. Lett. 68:175-180.
Pettmann, Weibel, Sensenbrenner, Labourdette (1985) Purification of two astroglial growth factors from bovine brain. FEBS Lett. 189:102-108.
Presta, Moscatelli, Joseph-Silverstein, Rifkin (1987) Purification from a human hepatoma cell line of a basic fibroblast growth factor-like molecule that stimulates capillary endothelial cell plasminogen activator production, DNA synthesis and migration. Mol. Cell. Biol. 6:4060-4066.
Sensenbrenner, Pettmann, Labourdette, Weibel (1985) Properties of a brain growth factor promoting proliferation and maturation of rat astroglial cells in culture. Hormones and Cell Regulation, Vol. 9, INSERM European Symposium , J.E. Dumont, B. Hamprecht, J. Nunez; 345-360.
Unkeless, Tobia, Ossowski, Quigley, Rifkin, Reich (1973) An enzymatic function associated with transformation of fibroblasts cultures transformed by avian RNA tumor viruses. J. Exp. Med. 137:85-111.
Weibel, Pettmann, Labourdette, Miehe, Bock, Sensenbrenner (1985) Morphological and biochemical maturation of rat astroglial cells grown in a chemically defined medium: influence of an astroglial growth factor. Dev. Neurosci. 3:617-630.
Westermark (1976) Density-dependent proliferation of human glial cells stimulated by epidermal growth factor. Biochem. Biophys. Res. Commun. 69:304-310.
