[en] Newborn rat dorsal root ganglia release two different plasminogen activators (PAs): the urokinase (UK) and the tissue (tPA) type. The former is secreted by neurons while the latter is secreted by Schwann cells. tPA release by Schwann cells is modulated by choleratoxin, a known mitogen for these cells. UK but not tPA stimulates in a dose-dependent fashion the proliferation of Schwann cells. This effect is observed in the absence of plasminogen, suggesting that the substrate for PAs in the developing nervous system is not plasminogen. Since UK is secreted by neurons, our data suggest a new mechanism for neuronal control of Schwann cell proliferation.
Leprince, Pierre ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Rogister, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Alvarez-Buylla, Valinsky (1985) Production of plasminogen activator in cultures of superior cervical ganglia and isolated Schwann cells. Proc. Natl. Acad. Sci. U.S.A. 82:3519-3523.
Baron-Van Evercooren, Kleinman, Seppa, Rentier, Dubois-Dalcq (1982) Fibronectin promotes rat Schwann cell growth and motility. J. Cell. Biol. 93:211-216.
Bottenstein, Sato (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc. Natl. Acad. Sci. U.S.A. 76:514-517.
Bottenstein (1986) Chemically defined media for growth and differentiation of CNS glial cells. Biochemistry of Glial Cells , T. Grisar, F. Franck, L. Hertz, W.T. Norton, M. Sensenbrenner, D.M. Woodbury, Pergamon, Oxford; 61:1-10.
Brockes, Fields, Raff (1979) Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerves. Brain Res. 165:105-118.
Deutsch, Mertz (1970) Plasminogen: purification from human plasma by affinity chromatography. Science 170:1095-1096.
Dubois-Dalcq, Rentier, Baron-Van Evercooren, Burge (1981) Structure and behaviour of rat primary and secondary Schwann cell in vitro. Exp. Cell Res. 131:283-297.
Engvall, Ruoslathi (1977) Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int. J. Cancer 20:20-25.
Guenther, Nick, Monard (1985) A glia-derived neurite promoting factor with protease inhibitory activity. EMBO J. 4:1963-1966.
Kalderon (1979) Migration of Schwann cells and wrapping of neurites in vitro; a function of protease activity (plasmin) in the growth medium. Proc. Natl. Acad. Sci. U.S.A. 76:5992-5996.
Kalderon (1984) Schwann cell proliferation and localized proteolysis. Expression of plasminogen activator activity predominates in the proliferating cell populations , Proc. Natl. Acad. Sci. U.S.A.; 81:7216-7220.
Keski-Oja, Vaheri (1982) The cellular target for the plasminogen activator, urokinase, in human fibroblasts is a 66,000 dalton protein. Biochim. Biophys. Acta 720:141-146.
Kreider, Messing, Doan, Kim, Lisak, Pleasure (1981) Enrichment of Schwann cell cultures from neonatal rat sciatic nerve by differential adhesion. Brain Res. 207:433-434.
Krystosek, Seeds (1981) Plasminogen activator secretion by granule neurons in cultures of developing cerebellum. Proc. Natl. Acad. Sci. U.S.A. 78:7810-7814.
Krystosek, Seeds (1984) Peripheral neurons and Schwann cells secrete plasminogen activator. J. Cell. Biol. 98:773-776.
Laemmli (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
McGarvey, Baron-Van Evercooren, Kleinman, Dubois-Dalcq (1983) Synthesis and effects of basement membrane components in cultured rat Schwann cells. The Role of Extracellular Matrix in Development , R.L. Trelstad, Liss, New York; 123-143.
Moonen, Grau-Wagemans, Selak (1982) Plasminogen activator-plasmin system and neuronal migration. Nature (London) 298:753-755.
Moonen, Grau-Wagemans, Selak, Lefebvre, Rogister, Vassalli, Belin (1985) Plasminogen activator is a mitogen for astrocytes in developing cerebellum. Dev. Brain. Res. 20:41-48.
Pittman (1985) Release of plasminogen activator and a calcium-dependent metalloprotease from cultured sympathetic and sensory neurons. Dev. Biol. 110:91-101.
Ouigley (1979) Phorbol ester induced morphological changes in transformed chick fibroblasts: evidence for direct catalytic involvement of plasminogen activator. Cell 17:131-141.
Ratner, Bunge, Glaser (1985) A neuronal cell surface heparin sulfate proteoglycan is required for dorsal root ganglion neuron stimulation of Schwann cell proliferation. J. Cell Biol. 101:744-754.
Saksela (1985) Plasminogen activation and regulation of pericellular proteolysis. Biochim. Biophys. Acta 823:35-65.
Salzer, Bunge, Glaser (1980) Studies of Schwann cell proliferation. Evidence for the surface localization of the neurite mitogen , J. Cell Biol.; 84:767-778.
Selak, Foidart, Moonen (1985) Laminin promotes cerebellar granule cells migration in vitro and is synthesized by cultured astrocytes. Dev. Neurosci. 7:278-285.
Unkeless, Tobia, Ossowski, Quigley, Rifkin, Reich (1973) An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses. J. Exp. Med. 137:85-111.
Vassali, Dayer, Wohlwend, Belin (1984) Concomittant secretion of pro-urokinase and of a plasminogen activator-specific inhibitor by cultured human monocytes-macrophages. J. Exp. Med. 159:1653-1668.
Wood, Bunge (1975) Evidence that sensory axons are mitogenic to Schwann cells. Nature (London) 256:662-664.
