[en] Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
China, B.
Goffaux, F.
Pirson, V.
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Comparison of Eae, Tir, Esp a and Esp b Genes of Bovine and Human Attaching and Effacing Escherichia Coli by Multiplex Polymerase Chain Reaction
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Mainil, J.G., Jacquemin, E., Kaeckenbeeck, A. and Pohl, P. (1993) Association between the effacing gene (eae) and the Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle. Am. J. Vet. Res. 54, 1064-1068.
China, B., Pirson, V. and Mainil, J. (1998) Prevalence and molecular typing of attaching and effacing Escherichia coli among calf populations in Belgium. Vet. Microbiol. 63, 249-259.
McDaniel, T.K., Jarvis, G., Donnenberg, M.S. and Kaper, J.B. (1995) A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl. Acad. Sci. USA 92, 1664-1668.
McDaniel, T.K. and Kaper, J.B. (1997) A cloned pathogenesis island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K12. Mol. Microbiol. 23, 399-407.
Elliott, S.J., Wainwright, L.A., McDaniel, T.K., Jarvis, K.G., Deng, Y.K., Lai, L.C., McNamara, B.P., Donnenberg, M.S. and Kaper, J.B. (1998) The complete sequence of the locus of enteroycte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol. Microbiol. 28, 1-4.
Perna, N., Mayhew, G.F., Posfai, G., Elliot, S., Donnenberg, M.S., Kaper, J.B. and Blattner, F.R. (1998) Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66, 3810-3817.
Karaolis, D.K.R., McDaniel, T.K., Kaper, J.B. and Boedeker, E.C. (1997) Cloning of the RDEC-1 locus of enterocyte effacement (LEE) and functional analysis of the phenotype on Hep-2 cells. In: Mechanisms in the Pathogenesis of Enteric Diseases (Paul, P.S., Francis, D.H. and Benfield, D.A., Eds.), Adv. Exp. Med. Biol. 412, pp. 241-245. Plenum, New York.
Goffaux, F., Mainil, J., Pirson, V., Charlier, G., Pohl, P., Jacquemin, E. and China, B. (1997) Bovine attaching and effacing Escherichia coli possess a pathogenesis island related to the LEE of the human enteropathogenic E. coli strain E2348/69. FEMS Microbiol. Lett. 154, 415-421.
Agin, T.S., Cantey, J.R., Boedeker, E.C. and Wolf, M.K. (1996) Characterization of the eaeA gene from rabbit enteropathogenic Escherichia coli RDEC-1 and comparison to other eaeA genes from bacteria that cause attaching-afacing lesions. FEMS Microbiol. Lett. 14, 249-258.
Abe, A., Kenny, B., Stein, M. and Finlay, B.B. (1997) Characterization of two virulence proteins secreted by rabbit enteropathogenic Escherichia coli, EspA and EspB, whose maximal expression is sensitive to host body temperature. Infect. Immun. 65, 3547-3555.
Paton, A.W., Mannig, P.A., Woodrow, M.C. and Paton, J.C. (1998) Translocated intimin receptor (Tir) of Shiga-toxigenic Escherichia coli isolates belonging to serogroups, O26, O111, and O157 react with sera from patients with hemolytic-Uremic syndrome and exhibit marked sequence heterogeneity. Infect. Immun. 66, 5580-5586.
An, H., Fairbrother, J.M., Dubreuil, D. and Harel, J. (1997) Cloning and characterization of the eae gene from a dog attaching and effacing Escherichia coli strain 4221. FEMS Microbiol. Lett. 148, 239-245.
China, B., Devrin, A.C., Jacquemin, E., Pirson, V. and Mainil, J. (1999) Heterogeneity of the eae genes in attaching/effacing Escherichia coli from cattle: comparison with human strains. Res. Microbiol. 150, 323-332.
Adu-Bobie, J., Frankel, G., Bain, C., Gonzales, A.G., Trabulsi, L.R., Douce, G., Knutton, S. and Dougan, G. (1998) Detection of intimins α, β, γ, and δ four intimin derivates expressed by attaching and effacing microbial pathogens. J. Clin. Microbiol. 36, 662-668.
Jerse, A.E., Yu, J., Tall, B.D. and Kaper, J.B. (1990) A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc. Natl. Acad. Sci. USA 87, 7839-7843.
Orskov, F. and Orskov, I. (1984) Serotyping of Escherichia coli. Method. Microbiol. 14, 43-112.
China, B., Pirson, V. and Mainil, J. (1996) Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl. Environ. Microbiol. 62, 3462-3465.
Blanco, M., Blanco, J.E., Blanco, J., Moro, A., Prado, C., Alonso, M.P., Mourino, M., Madrid, C., Balsalobre, C. and Juarez, A. (1997) Distribution and characterization of faecal verotoxin-producing Escherichia coli (VTEC) isolated from healthy cattle. Vet. Microbiol. 54, 309-319.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
