[en] In response to stressors involving tissue injury, pituitary corticotroph secretion of immunoreactive beta-endorphin (iB-END) could be either due to release of hypothalamic factors such as corticotropin-releasing factor (CRF) or to release of a tissue factor from the periphery. In the present experiments, we investigated whether inflamed tissue releases a factor which evokes pituitary secretion of iB-END. In an initial experiment, rats with an inflamed hindpaw due to carrageenan injection had significantly greater levels of circulating iB-END as compared to rats with saline-injected paws. Removal of afferent input, by hindlimb denervation, failed to block the carrageenan-induced increase in iB-END levels. Subcutaneous perfusates were then collected from inflamed and control hindlimbs and applied to rat anterior pituitary cell cultures. Pituitary release of iB-END due to administration of perfusate from inflamed paws was significantly greater than iB-END release due to perfusate from saline-injected paws or to basal release. The releasing activity in the perfusates was blocked in calcium-free medium and was not due to a direct action of carrageenan, bradykinin, substance P or calcitonin gene-related peptide. The results indicate that inflamed tissue releases a CRF-like factor which stimulates iB-END release both in the denervated rat and cultured pituitary cells.
Disciplines :
Anesthesia & intensive care
Author, co-author :
Hargreaves, K. M.
Costello, A. H.
Joris, Jean ; Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation
Language :
English
Title :
Release from inflamed tissue of a substance with properties similar to corticotropin-releasing factor.
Aki, R; Shiomi H.; Walker, J.; Watson S.: Characterization of multiple forms of beta endorphin in pituitary and brain: Effects of stress. In; Regulatory Peptides: From Molecular Biology to Function, Costa, E.; Trabucchi, M., editors, pp. 61-67 (Raven Press. New York 1982)
Banter, J.; Tyrell J.: The adrenal cortex. In: Endocrinology and Metabolism. Felig. P.; Baxter. J.; Broadus. A,; Froam, L, editors, pp. 510-550 (McGraw-Hill, New York 1981).
Iierkenbosch, F; Van Oers, J.; Del Ray, A.; TiLders, F.; Besedovsky, H.: Corticotropin releasing factor producing neuron in the rat activated by imerleukin I. Science 238:524-526 (1988).
Bemton, E.; Beach, J.; Holaday, J.; Smallridge, R.; Peine, V.; Release of multiple hormones by direct action of interferon I on pituitary ceils. Science. 23,1: 519-521 (1987)
Hluloek, J.; Smith E.: Human leukocyte interferon: Structural and biological relaicdness to adrenocorticotropic hormone and endorphins. Proc. natn. Acad, Sci. USA 77:5972-5974 (1980).
Carr, D.; Osgood P.; Szyfelbein, S.: Bum-induced analgesia and endorphin secretion: Analysis in a pituitary autotransplaul model, (abstract). Pain suppL 4, p. S4I3 (1987).
De Souza, E.; Van Loon. G.: Differential plasma beta endorphin, beta lipotropin. and adrenocorticotropin response to stress in rats. Endocrinology 116: 1577-1586 (1985).
DiAugusline, L; Lazarus, G.; Jahnke, G., Kahn, M.; Erisman, M.; Linnoila, R.: Corricotropin/beta-endorphin immunoreactivity in rat mast cells: peptide or protease. Life Sci. 27:2663-2668 (1980).
Farah J.; Hall, N.; Bishop, J; Donohue, T.: Thymosin traction V stimulates secretion of immunoreaetive beta endorphin in mouse corticotropic tumor cells. J. neurosci. Res. 18: 140-146 (1987).
Flower, R.; Parente, L.; Peisico, P.; Salmon, J,: A comparison of the acute inflammatory response in adrenalectomised and sham-operated rats. Br. J. Pharmacol, 87:57-62 (1986).
Gordon, M.: An evaluation of afferent nervous impulses in the adrenal correal response to trauma. Endocrinology 47:347-350 (1950).
Hargreaves, K.M.; Dionne, R.A.; Mueller, G.P.; Goldstein, D.; Dubner, R.: Naloxone, fentany! and diazepam modify plasma beta-endorphin levels during surgery. Clin. Pharmacol, Ther, 40: 165-171 (1986).
Hargreaves, K.M.; Dubner, R., Brown, F.; Flores, O; Joris, J.: A new and sensitive method lor measuring thermal nociception in cutaneous hyperalgesia. Rain 32:77-88 (1988).
Jahanke, G.; Lazarus, L.; DiAugusline, R.; Soldatao C; Erisman, M.: Peptide hormone degradation by a rat mast cell chymase-hepa-rin complex. Life Sci. 29:397-103 (1981).
Joris J., Costello, A.; Hargreaves, K.M.: Local production of immunoreaetive bradykinin (iBK) in two models of inflammation (Abstract). Pain suppl. 4, p. S17 (1987).
Luger, A; Calogero, A.; Kalogeras, K.; Gallucci, W; Gold, P.; Loriatix, L.; Chrousos, G.: Interaction of epidermal growth factor with the hypothalamic pituitaiy adrenal axis: potential physiologic relevance, J. din. Endocr. Melab, 66. 334-337 (1988).
Lymangrover, J.R., Brodish, A.: Tissue CRF: an extra-hypo thalamic corticotropin releasing Tactor (CRF) in the peripheral blood of stressed rats, Ncuroendoerinology 12:225-235 (1973).
Matsumura M., Ohura M.; Shimizu, L.; Yamoni, A.; Iwasaki, A.; Saito, S.: In vivo and in vllro effects ofbradykinin on the release of beta-cndorphin-like immunoreaetivity. Neuroendocrinology 41: 101-106(1985).
Meek, J.; Rosetti, Z,: Factors affecting retention and resolution of peptides in high performance liquid chromatography. J. Chroma-togr. 211: 15-28 (1981).
Mezey, E.; Reisine, T.; Brownstein, M., Lalkovits M; Axetrod, L.: Beta adrenergic mechanism of insulin-induced adrenocortieotropin release from anterior pituitary. Science 226: 1085-1087 (1984).
Milian, M.; Milian, M.; Członkowski, A.; Hollt, V.; Pilcher, C; Her, A.; Colpaen, F.: A model of chronic pain in the ral: Responses of multiple opioid system to adjuvant induced arthritis, J. Neurosci. 6: 899-906 (1986).
Mueller, G.P.: Attenuated pituitary beta-endorphin release in estrogen treated rats. Proc. Soc. exp. Biol. Med. 165: 75-81 (1980).
Mueller, G.L.: Beta-endorphin iinmunoreactivity in rat plasma; variations in response to different physical stimuli. Life Sci. 29: 1669-1674(1981).
Nakane T.; Audhya, T; Kanie, N.; Hollander, C: Evidence for a role of endogenous CRF in cold, ether, immobilization and traumatic stress. Proc. natn. Acad. Sci. VSAS: 1247-1251 (1985).
Okajima T.: Hertting, G.; Bradykinin-induced ACTH release from rat pituitary tissue in vitro. Neuroendocrinology 43: 336-339 (1986).
Pettibone D.; Mueller, G.: Alpha adrenergic stimulation by cloni-dine increases plasma concentrations of immunoreactive beta endorphin in rats. Endocrinology 109:798-802 (1981).
Rivier J.; Rivier, C; Vale W.: Synthetic competitive antagonists of corticotropin releasing Factor: Effect of ACTH secretion in the rat Science 224 889-891 (1984).
Sapolsky R.; Rivier, C: Yamamoto G.; Plotsky P.; Vale, W.: In-terleukin I stimulates the secretion Of hypothalamic corticotropin-releasing factor. Science 238:522-524 (1987).
Simpson, C.W.; Hamlet, M.; Brodish, A.; ACTH regulation of lis-sue-CRF. Neuroendocrinology 31:210-214(1980).
Tsubouchi H.; Miyazaki, H.; Gohda, Bi; Nakayama, H.; Na-Kazonon, Y., Daikuhara Y.; Hashimoto, S.: Degradation of liodoglucagon by normal rat plasma in radioimmunoassay mix-lure containing aprotinin and its prevention by-chloromercuriphenyl sulfonate and leupeplin. Endocrinology 119: 1137-1145 (1986).
Vale, W.; Grant, G.; Amoss, M; Blackwell, R; Guillemin, R.: Culture of enzymatic.ilIy dispersed anterior pituitary cells: Functional validation of a method. Endocrinology 91: 562-572 (1972).
Vale W. Spies: J Rivier C Rivier J Characltn ynicn of a 41 residue ovine hypothalamic peptide thai stimulates secretion of corticotropin and beta endorphin. Science 213: 1394-1397(1981).
Winer B.: Statistical principles in experimental design (McGraw-Hill, New York 1971).
