[en] Reaction of yeast RNA polymerase I with pyridoxal 5′-phosphate and sodium borohydride under conditions which inactivate the enzyme results in the specific binding of pyridoxal 5′-phosphate to subunits of 185,000, 137,000, 48,000 and 36,000 daltons. Nucleotides, which protect the enzyme from inactivation specifically inhibit the binding of pyridoxal 5′-phosphate to subunits of 185,000 and 137,000 daltons. DNA which also protects the enzyme from inactivation by pyridoxal 5′-phosphate prevents the binding of the reagent to the four polypeptides.
These results suggest that subunits of 185,000 and 137,000 are involved in interactions with both nucleotides and DNA presumably of the type leading to initiation and/or polymerization and that subunits of 48,000 and 36,000 daltons also bind to DNA but this interaction is not strictly required for polymerase activity.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Valenzuela, Pablo
Bull, Paulina
Zaldivar, Josefina
Venegas, Venegas
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Subunits of yeast RNA polymerase I involved in interactions with DNA and nucleotides
Publication date :
1978
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
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Bibliography
Chambon Ann. Rev. Biochem 1975, 44:613-638.
Roeder RNA Polymerase , R. Losick, M. Chamberlin, Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.; 1976, 285-329.
Valenzuela, Hager, Weinberg, Rutter Proc. Nat. Acad. Sci. USA 1976, 73:1024-1028.
Valenzuela, Weinberg, Bell, Rutter J. Biol. Chem 1976, 251:1464-1470.
Buhler, Sentenac, Fromageot J. Biol. Chem 1974, 249:5963-5970.
Dezelee, Wyers, Sentenac, Fromageot Eur. J. Biochem 1976, 65:543-552.
Valenzuela, Bell, Weinberg, Rutter Methods in Cell Biology, Chromosomal Protein Research , G.S. Stein, J.L. Stein, L.J. Kleinsmith, Academic Press, New York, in press; 1977, 18.
Buhler, Dezelee, Huet, Iborra, Sentenac J. Biol. Chem 1976, 251:1712-1717.
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