[en] Analysis of GH gene structure in the mouse permits evolutionary comparisons with GH gene structure in the rat and provides information about the mechanism responsible for heritable deficiencies of anterior pituitary hormones. The little mutation in mice is analogous to autosomal recessive, isolated, partial deficiency of GH in man, whereas the Snell dwarf mutation is a model for autosomal recessive deficiency of GH, TSH, and PRL. Mouse cellular DNA was digested with the restriction enzymes Eco RI, Bam HI, Bgl II, Hind III, and Kpn I, singly and in combination. Gene sequences containing coding information for GH were detected by hybridization to a radiolabeled recombinant DNA probe complementary to a rat GH mRNA. The results of genomic restriction analysis indicate that there is a single type of mouse GH gene sequence per haploid genome with a length equal to or less than 32000 base pairs. The distances separating 6-base restriction sites close to the mouse GH gene differ from those close to the rat GH gene. A Kpn I site in the codons for amino acids 103 and 104 of rat GH is absent in the mouse gene. Restriction patterns of DNA from little and Snell dwarf mice did not differ from those of normal mice, indicating that these mutations do not involve deletions of the mouse GH gene.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Parks, J. S.
Herd, J. E.
Wurzel, J. M.
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Structural analysis of rodent growth hormone genes: application to genetic forms of hypopituitarism
Publication date :
1982
Journal title :
Endocrinology
ISSN :
0013-7227
eISSN :
1945-7170
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Snell GD 1929 Dwarf, a new mendelian recessive character of the house mouse. Proc Natl Acad Sci USA 15:733
Carsner RL, Rennels EG 1960 Primary site of gene action in anterior pituitary dwarf mice. Science 131:829
Eicher EM, Beamer WG 1980 New mouse dw allele: genetic location and effects on lifespan and growth hormone levels. J Hered 71:187
Seeburg PH, Shine J, Martial JA, Baxter JD, Goodman HM 1977 Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature 270:486
Schneider JA, Weiss MC 1971 Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine amino transferase in hepatoma fibroblast hybrids. Proc Natl Acad Sci USA 68:127
Dubbs DR, Kit S 1964 Effect of halogenated pyrimidines and thymidine on growth of L-cells and a subline lacking thymidine kinase. Exp Cell Res 33:19
Kan YW, Dozy AM, Trecartin MS, Todd D 1977 Identification of a nondeletion defect in a-halassemia. N Engl J Med 297:1081
Southern EM 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503
Rigby PWS, Dieckman M, Rhodes C, Berg P 1977 Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237
Wahl JM, Stern M, Stark GR 1979 Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76:3683
Chien Y, Thompson EB 1980 Genomic organization of rat prolactin and growth hormone genes. Proc Natl Acad Sci USA 77:4583
Barta A, Richards RI, Baxter JD, Shine J 1981 Primary structure and evolution of the rat growth hormone gene. Proc Natl Acad Sci USA 78:4867
Goodman HM, DeNoto F, Fiddes JC, Hallewell RA, Page GS, Smith S, Tischer H 1980 Structure and evolution of growth hormone related genes. In: Scott WA (ed) Mobilization and Reassembly of Genetic Information: Proceedings of the Miami Winter Symposium. Academic Press, New York, p 155
Phillips III. JA, Hjelle BL, Seeburg PH, Zachmann M 1981 Molecular basis for familial isolated growth hormone deficiency. Proc Natl Acad Sci USA 78:6372
Merimee TJ, Rimoin DL, Hall JD, McKusick VA 1969 A metabolic and hormonal basis for classifying ateliotic dwarfs. Lancet 1:963
Parks JS, Herd JE, Wurzel JM 1981 Human growth hormone deficiency and polymorphism within the hgh and human placental lactogen gene cluster. Pediatr Res 15:513 (Abstract)
Illig R 1970 Growth hormone antibodies in patients treated with different preparations of human growth hormone. J Clin Endocrinol Metab 31:679
Eicher EM, Beamer WG 1976 Inherited ateliotic dwarfism in mice: characteristics of the mutation, little, on chromosome 6. J Hered 67:87