Reference : Thyroid hormone receptors bind to defined regions of the growth hormone and placental...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/26656
Thyroid hormone receptors bind to defined regions of the growth hormone and placental lactogen genes
English
Barlow, John W [> > > >]
Voz, Marianne mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Eliard, Pierre H [> > > >]
Mathy, Marianne [Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
De Nayer, Philippe [> > > >]
Economidis, Ioannis V [> > > >]
Belayew, Alexandra [> > > >]
Martial, Joseph mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Rousseau, Guy G [> > > >]
1986
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences
83
23
9021-5
Yes (verified by ORBi)
International
0027-8424
1091-6490
Washington
DC
[en] Base Sequence ; Binding Sites ; Cell Nucleus/metabolism ; DNA-Binding Proteins/physiology ; Gene Expression Regulation ; Growth Hormone/*genetics ; Humans ; Placental Lactogen/*genetics ; Promoter Regions, Genetic ; Receptors, Thyroid Hormone/*physiology ; Triiodothyronine/*physiology
[en] The intracellular receptor for thyroid hormone is a protein found in chromatin. Since thyroid hormone stimulates transcription of the growth hormone gene through an unknown mechanism, the hypothesis that the thyroid hormone-receptor complex interacts with defined regions of this gene has been investigated in a cell-free system. Nuclear extracts from human lymphoblastoid IM-9 cells containing thyroid hormone receptors were incubated with L-3,5,3'-tri[125I]iodothyronine and calf thymus DNA-cellulose. Restriction fragments of the human growth hormone gene were added to determine their ability to inhibit labeled receptor binding to DNA-cellulose. These fragments encompassed nucleotide sequences from about three kilobase pairs upstream to about four kilobase pairs downstream from the transcription initiation site. The thyroid hormone-receptor complex bound preferentially to the 5'-flanking sequences of the growth hormone gene in a region between nucleotide coordinates -290 and -129. The receptor also bound to an analogous promoter region in the human placental lactogen gene, which has 92% nucleotide sequence homology with the growth hormone gene. These binding regions appear to be distinct from those that are recognized by the receptor for glucocorticoids, which stimulate growth hormone gene expression synergistically with thyroid hormone. The presence of thyroid hormone was required for binding of its receptor to the growth hormone gene promoter, suggesting that thyroid hormone renders the receptor capable of recognizing specific gene regions.
http://hdl.handle.net/2268/26656
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3466175
1986/12/01

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