[en] Differential display analysis was carried out to find, in the rat prostate, genes that could be regulated by Luteinizing Hormone (LH), independently of the androgens. Hypophysectomized and castrated adult rats were treated with either LH, testosterone or saline. Regulated discrete bands have been eluted and reamplified. After Northern blotting, the levels of mRNA corresponding to 8 PCR fragments were significantly increased by LH treatment. None of these inserts were found to be induced by testosterone. One insert was subcloned, sequenced and identified as the ribosomial protein S 23. A competitive RT-PCR assay was carried out on the full length S 23 cDNA and confirmed that its mRNA levels were stimulated by LH but not by testosterone. These results strongly suggest that the LH membrane receptor, previously shown to be expressed in the rat prostate, has a physiological significance in this organ. Moreover, it appears that the effect of LH on the rat prostate are independent of the androgens.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Reiter, E.
Poncin, Joseph ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
1. Cunha, G. R., Donjacour, A. A., Cooke, P. S., Mee, S., Bigsby, R. M., Higgins, S. J., and Sugimura, Y. (1987) The endocrinology and developmental biology of the prostate. Endocr. Rev. 8, 338-362.
2. Wilding, G. (1995) Endocrine control of prostate cancer. Canc. Surv. 23, 43-62.
3. Grayhack, J. T., Bunce, P. L., Kearns, J. W., and Scoot, W. W. (1955) Influence of the pituitary on prostatic response to androgen in the rat. Bull. Johns Hopkins Hosp. 96, 154-321.
4. Aragona, C., and Friesen, H. G. (1975) Specific prolactin binding site in the prostate and testis of rats. Endocrinol. 97, 677-684.
5. Yamanaka, H., Kirdani, R. Y., Saroff, J., Murphy, G. P., and Sandberg, A. A. (1975) Effects of testosterone and prolactin on rat prostatic weight, 5α-reductase and arginase. Am. J. Physiol. 229, 1102-1110.
6. Prins, G. S. (1987) Prolactin influence on cytosol and nuclear androgen receptors in the ventral, dorsal and lateral lobes of the rat prostate. Endocrinol. 120, 1457-1464.
7. Franklin, R. B., and Costello, L. C. (1992) Prolactin stimulates transcription of mitochondrial aspartate aminotransferase of prostate epithelial cells. Mol. Cell. Endocrinol. 90, 27-32.
8. Reiter, E., Lardinois, S., Klug, M., Sente, B., Hennuy, B., Bruyninx, M., Closset, J., and Hennen, G. (1995) Androgen-independent effects of prolactin on the different lobes of the rat prostate. Mol. Cell. Endocrinol. 112, 113-122.
9. Reiter, E., Bonnet, P., Sente, B., Dombrowicz, D., de Leval, J., Closset, J., and Hennen, G. (1992) Growth hormone and prolactin stimulate androgen receptor, insulin-like growth factor-I (IGF-I) and IGF-I receptor levels in the prostate of immature rats. Mol. Cell Endocrinol. 88, 77-87.
10. Reiter, E., Kecha, O., Hennuy, B., Lardinois, S., Klug, M., Bruyninx, M., Closset, J., and Hennen, G. (1995) Growth Hormone directly affects the function of the different lobes of the rat prostate. Endocrinol. 136, 3338-3345.
11. Reiter, E., McNamara, M., Closset, J., and Hennen, G. (1995) Expression and functionality of luteinizing hormone/chorionic gonadotropin receptor in the rat prostate. Endocrinol. 136, 917-923.
12. Tao, Y. X., Bao, S., Ackermann, D. M., Lei, Z. M., and Rao, C. V. (1997) Expression of luteinizing hormone/human chorionic gonadotropin receptor gene in benign prostatic hyperplasia and in prostate carcinomas in humans. Biol. Reprod. 56, 67-72.
13. Ghosh, P. K., and Bartke, A. (1993) Effects of the expression of bovine growth hormone on the testes and male accessory reproductive glands in transgenic mice. Transgenic Res. 2, 79-83.
14. Nguyen, A. P., Chandorkak, A., and Gupta, C. (1996) The role of growth hormone in fetal mouse reproductive tract differentiation. Endocrinol. 137, 3659-3666.
15. Rao, C. V. (1996) The beginning of a new era in reproductive biology and medicine: Expression of low levels of functional luteinizing hormone/human chorionic gonadotropin receptors in non gonadal tissues. J. Physiol. Pharmacol. 47, 41-53.
16. Liang, P., and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967-971.
17. Liang, P., Averboukh, L., and Pardee, A. B. (1993) Distribution and cloning of eukaryotic mRNAs by means of differential display: Refinements and optimization. Nucleic Acid Res 21, 3269-3275.
18. Chomczinski, P., and Sacchi, N. (1987) Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
19. Segaloff, D. L., and Ascoli, M. (1993) The lutropin/choriogonadotropin receptor . . . 4 years later. Endocr. Rev. 14, 324-347.
20. Kitaoka, Y., Olvera, J., and Wool, I. G. (1994) The primary structure of rat ribosomal protein S23. Biochem. Biophys. Res. Comm. 202, 314-320.
21. Forster, C. (1994) Rapid generation of internal standards for competitive PCR by low-stringency primer annealing. Biotechniques 16, 1006-1008.
22. Kim, Y. I., Ahn, H.-J., Zelner, D. J., Park, L., Sensibar, J. A., and Lee, C. (1996) Expression and localization of transforming growth factor-β receptors type I and type II in the rat ventral prostate during regression. Mol. Endocrinol. 10, 107-115.
23. Neumann, F., Hemmerich, P., von Mikecz, A., Peter, H.-H., and Krawinkel, U. (1995) Human ribosomal protein L7 inhibits cell-free translation in reticulocyte lysates and affects the expression of nuclear proteins upon stable transfection into jurkat T-lymphoma cells. Nucl. Acid. Res. 23, 195-202.
24. Shimbara, N., Sato, C., Takashina, M., Tanaka, T., Tanaka, K., and Ichihara, A. (1993) Down-regulation of ubiquitin gene expression during differentiation of human leukemia cells. FEBS Lett. 322, 235-239.
25. Marechal, V., Elenbaas, B., Piette, J., Nicholas, J.-C., and Levine, A. J. (1994) The ribosomal L5 protein is associated with mdm-2 and mdm-2-P53 complexes. Mol. Cell. Biol. 14, 7414-7420.
