Reference : Luteinizing hormone increases the abundance of various transcripts, independently of ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Luteinizing hormone increases the abundance of various transcripts, independently of the androgens, in the rat prostate.
Reiter, E. [> > > >]
Poncin, Joseph [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Hennuy, Benoît mailto [Université de Liège - ULiège > > GIGA-Management : Plate-forme transcriptomique >]
Bruyninx, M. [> > > >]
Klug, M. [> > > >]
Cornet, Anne [Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Closset, Josiane [Centre Hospitalier Universitaire de Liège - CHU > > Secteur prélèvements sanguins >]
Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
Biochemical and Biophysical Research Communications
Academic Press
Yes (verified by ORBi)
San Diego
[en] Animals ; Gene Expression Regulation/drug effects ; Hypophysectomy ; Luteinizing Hormone/pharmacology ; Male ; Orchiectomy ; Prostate/physiology ; RNA, Messenger/genetics/metabolism ; Rats ; Rats, Wistar ; Testosterone/physiology
[en] Differential display analysis was carried out to find, in the rat prostate, genes that could be regulated by Luteinizing Hormone (LH), independently of the androgens. Hypophysectomized and castrated adult rats were treated with either LH, testosterone or saline. Regulated discrete bands have been eluted and reamplified. After Northern blotting, the levels of mRNA corresponding to 8 PCR fragments were significantly increased by LH treatment. None of these inserts were found to be induced by testosterone. One insert was subcloned, sequenced and identified as the ribosomial protein S 23. A competitive RT-PCR assay was carried out on the full length S 23 cDNA and confirmed that its mRNA levels were stimulated by LH but not by testosterone. These results strongly suggest that the LH membrane receptor, previously shown to be expressed in the rat prostate, has a physiological significance in this organ. Moreover, it appears that the effect of LH on the rat prostate are independent of the androgens.

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