A new natural hGH variant--17.5 kd--produced by alternative splicing. An additional consensus sequence which might play a role in branchpoint selection

Lecomte, C. M.; Renard, A.; Martial, Joseph

doi:10.1093/nar/15.16.6331

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A new natural hGH variant--17.5 kd--produced by alternative splicing. An additional consensus sequence which might play a role in branchpoint selection

Lecomte, C. M.; Renard, A.; Martial, Joseph

1987 • In Nucleic Acids Research, 15 (16), p. 6331-48

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https://hdl.handle.net/2268/23700

DOI
10.1093/nar/15.16.6331

PubMed
3627992

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Keywords :

Base Sequence; Chorionic Gonadotropin/*genetics; DNA/genetics; Genes; Genes, Regulator; Humans; Molecular Weight; Pituitary Neoplasms/analysis; *RNA Splicing; RNA, Neoplasm/genetics/isolation & purification; Recombinant Proteins/genetics

Abstract :

[en] From a human pituitary cDNA library, we have cloned 3 distinct human growth hormone (hGH) cDNAs, coding respectively for the 22 K hGH, the 20 K variant, and a yet unknown 17.5 K variant. S1 mapping analysis using human pituitary RNA confirms the existence of at least four distinct hGH mRNAs originating from alternative acceptor sites at the second intron of the primary transcript. We have analysed the hGH gene sequence to explain the high frequency of alternative splicings which occur only at this location. In this study we propose CTTGNNPyPyPy as an additional consensus sequence guiding the selection of the branched nucleotide.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Lecomte, C. M.

Renard, A.

Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Language :

English

Title :

A new natural hGH variant--17.5 kd--produced by alternative splicing. An additional consensus sequence which might play a role in branchpoint selection

Publication date :

1987

Journal title :

Nucleic Acids Research

ISSN :

0305-1048

eISSN :

1362-4962

Publisher :

Oxford University Press, Oxford, United Kingdom

Volume :

Issue :

Pages :

6331-48

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.pubmedcentral.nih.gov/pagerender.fcgi?artid=306109&pageindex=1

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since 24 September 2009

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