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Article (Scientific journals)
Discrepancy between prolactin (PRL) messenger ribonucleic acid and PRL content in rat fetal pituitary cells: possible role of dopamine
Hooghe-Peters, E. L.; Belayew, A.; Herregodts, P. et al.
1988In Molecular Endocrinology, 2 (12), p. 1163-8
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Keywords :
Animals; Dopamine/analysis/*physiology; Fetus/analysis; Gene Expression Regulation; Growth Hormone/analysis/genetics/metabolism; Immunoenzyme Techniques; Nucleic Acid Hybridization; Pituitary Gland/*analysis/embryology; Prolactin/analysis/*genetics/metabolism; RNA/analysis; RNA, Messenger/*analysis; Rats
Abstract :
[en] Data are controversial concerning the time when PRL-synthesizing cells are detected for the first time in the rat pituitary. Using a very sensitive immunocytochemical technique, we could visualize only a few PRL cells before day 10 after birth. At that time, pituitary PRL was still 200 times less abundant than in the adult (on a tissue weight basis) whereas PRL mRNA per mg total RNA was only 80 times lower than in the adult. However, by in situ hybridization, we could demonstrate the presence of PRL mRNA in cells from fetal day 18 on. We have also followed the expression of GH gene in rat pituitary cells during development. In contrast to results obtained with PRL cells, quantitative analysis of cDNA probe hybridization to GH mRNA correlated well with measurements of immunostained cells. We found that PRL was released in the blood from fetal day 19 onwards. Thus, at that time PRL is synthesized and secreted but not stored. We therefore measured brain dopamine levels, and the data support the idea that the rise in dopamine levels after birth contributes to PRL storage. We confirmed in vitro that newborn pituitary cells can store PRL when cultured in the presence of dopamine.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Hooghe-Peters, E. L.
Belayew, A.
Herregodts, P.
Velkeniers, B.
Smets, G.
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Vanhaelst, L.
Language :
English
Title :
Discrepancy between prolactin (PRL) messenger ribonucleic acid and PRL content in rat fetal pituitary cells: possible role of dopamine
Publication date :
1988
Journal title :
Molecular Endocrinology
ISSN :
0888-8809
eISSN :
1944-9917
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Volume :
2
Issue :
12
Pages :
1163-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 September 2009

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