[en] Data are controversial concerning the time when PRL-synthesizing cells are detected for the first time in the rat pituitary. Using a very sensitive immunocytochemical technique, we could visualize only a few PRL cells before day 10 after birth. At that time, pituitary PRL was still 200 times less abundant than in the adult (on a tissue weight basis) whereas PRL mRNA per mg total RNA was only 80 times lower than in the adult. However, by in situ hybridization, we could demonstrate the presence of PRL mRNA in cells from fetal day 18 on. We have also followed the expression of GH gene in rat pituitary cells during development. In contrast to results obtained with PRL cells, quantitative analysis of cDNA probe hybridization to GH mRNA correlated well with measurements of immunostained cells. We found that PRL was released in the blood from fetal day 19 onwards. Thus, at that time PRL is synthesized and secreted but not stored. We therefore measured brain dopamine levels, and the data support the idea that the rise in dopamine levels after birth contributes to PRL storage. We confirmed in vitro that newborn pituitary cells can store PRL when cultured in the presence of dopamine.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Hooghe-Peters, E. L.
Belayew, A.
Herregodts, P.
Velkeniers, B.
Smets, G.
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Vanhaelst, L.
Language :
English
Title :
Discrepancy between prolactin (PRL) messenger ribonucleic acid and PRL content in rat fetal pituitary cells: possible role of dopamine
Publication date :
1988
Journal title :
Molecular Endocrinology
ISSN :
0888-8809
eISSN :
1944-9917
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Rennels EG, Herbert DC, 1980 Functional correlates of anterior pituitary cytology. Int Rev Physiol 222:1-40
Velkeniers B, Hooghe-Peters E, Hooghe R, Belayew A, Smets G, Robberecht P, Vanhaelst L, 1988 Prolactin cell subpopulations separated on discontinuous Percoll gra-dient: an immunocytochemical, biochemical, and physio-logical characterization. Endocrinology 123:1619-1630
Laverriere JN, Gourdji JN, Picart R, Tixier-Vidal A, 1981 Thyroliberin is rapidly transferred to the nucleus of GH3 pituitary cells at both 4 degree C and 37 degree C. Embo J 103:833-840
MacLeod RM, 1976 In: Martini L, Ganong F (eds) Frontiers in Neuroendocrinology. Raven Press, New York, p 129
Setalo G, Nakane PK, 1976 Functional differentiation of the fetal anterior pituitary cells in the rat. Endocrinol Exp (Bratisl) 10:155-166
Ingleton PM, Ensor DM, Hancock MP, 1971 Identification of prolactin in foetal rat pituitary. J Endocrinol 51:799-800
Chatelain A, Dupouy JP, Dubois MP, 1979 Ontogenesis of cells producing polypeptide hormones (ACTH, MSH, LPH, GH, PRL) in foetal hypophysis of the rat: influence of the hypothalamus. Cell Tissue Res 196:409-427
Watanabe YG, Daikoku S, 1979 An immunocytochemical study on the cytogenesis of adenohypophyseal cells in foetal rats. Dev Biol 68:557-567
Smets G, Velkeniers B, Finne E, Baldys A, Gepts W, Vanhaelst L, 1987 Ontogeny of hormone secreting cells of the rat pituitary gland: an immunocytochemical study on single cells. J Histochem Cytochem 35:335-341
Gluckman PD, Grumbach MM, Kaplan SL, 1981 The neu-roendocrine regulation and function of growth hormone and prolactin in the mammalian fetus. Endocr Rev 2:363-395
Puymirat J, Barret A, Faivre-Bauman A, Tixier-Vidal A, 1988 Biochemical characterization of the uptake and re-lease of dopaminergic hypothalamic neurons: a develop-ment study using serum-free medium cultures. Dev Biol 199:75-84
Ojeda SR, McCann SM, 1974 Development of dopami-nergic and estrogenic control of prolactin release in the female rat. Endocrinology 95:1499-1505
Vijayan E, McCann SM, 1978 The effect of systemic administration of dopamine and apomorphine on plasma LH and prolactin concentrations in conscious rats. Neu-roendocrinology 25:221-235
Hooghe-Peters EL, Fowlkes BJ, Hooghe R, 1979 A new neuronal marker identified by phosphorylcholine-binding myeloma proteins. Nature 281:376-378
Sternberger LA, 1979 Immunocytochemistry, ed. 2. John Wiley & Sons, New York, p 104
Seeburg PH, Shine J, Martial JA, Baxter JD, Goodman HM, 1977 Nucleotide sequence and amplification in bac-teria of structural gene for rat growth hormone. Nature 270:486-494
Cooke NE, Coit D, Weiner RI, Baxter JD, Martial JA 1980 Structure of cloned DNA complementary to rat prolactin messenger RNA. J Biol Chem 255:6502-6510
Preston CM, 1977 The cell-free synthesis of Herpes virus induced polypeptides. Virology 78:349-353
Rigby PWJ, Dieckmann M, Rhodes C, Berg P, 1977 La-beling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase. J Mol Biol 113:237-251
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ, 1979 Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299
Maniatis T, Fritsch EF, Sambrook J, 1982 Molecular Clon-ing. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p 202
Thomas PS, 1980 Hybridization of denatured RNA abs small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77:5201-5205
Herregodts P, Michotte Y, Ebinger G, 1985 Determination of the biogenic amines and their major metabolites in single human brain tissue samples using a combined extraction procedure and high performance liquid chro-matography with electrochemical detection. J Chromatogr 345:33-42
Greenwood FC, Hunter WH, Glover JS, 1963 The prepa-ration of131l-labelled human growth hormone of high specific radioactivity. Biochem J 89:114-123
Zanini A, Giannattasio G, Meldolesi J, 1974 Separation of rat pituitary growth hormone and prolactin by SDS poly-acrylamide gel electrophoresis. Endocrinology 94:594-598
