Reference : The influence of follicular dendritic cells on B-cell proliferation depends on the ac...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
The influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used.
Bosseloir, A. [> > > >]
Bouzahzah, F. [> > > >]
Defrance, T. H. [> > > >]
Heinen, Ernst mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine >]
Simar, L. J. [> > > >]
Scandinavian Journal of Immunology
Blackwell Publishing
Yes (verified by ORBi)
United Kingdom
[en] Antibiotics, Antineoplastic/pharmacology ; Antibodies, Monoclonal/pharmacology ; Antigens, CD40/immunology ; B-Lymphocytes/immunology ; Cell Division ; Cell Separation ; Cells, Cultured ; Child ; Child, Preschool ; DNA Replication ; Dendritic Cells/immunology ; Humans ; Immunoglobulin G/immunology ; Lymphocyte Activation/drug effects ; Mitogens/pharmacology ; Mitomycin/pharmacology ; Palatine Tonsil/cytology ; Staphylococcus aureus/immunology ; Transferrin/immunology
[en] Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolated them from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12-16 h remained attached to the substrate; non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but not when stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC in the presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulant encountered.

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