Reference : Isolation of follicular dendritic cells from human tonsils and adenoids. III. Analysi...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Isolation of follicular dendritic cells from human tonsils and adenoids. III. Analysis of their Fc receptors.
Heinen, Ernst mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine >]
Radoux, D. [ > > ]
Kinet-Denoel, C. [> > > >]
Moeremans, M. [> > > >]
De Mey, J. [> > > >]
Simar, L. J. [> > > >]
Blackwell Publishing
Yes (verified by ORBi)
United Kingdom
[en] Adenoids/analysis/cytology/ultrastructure ; Antibodies/analysis ; Humans ; Microscopy, Electron ; Palatine Tonsil/analysis/cytology/ultrastructure ; Receptors, Complement/analysis ; Receptors, Complement 3b ; Receptors, Fc/analysis
[en] Follicular dendritic cells (FDC), isolated from human tonsils or adenoids, were tested for their capacity to retain monomeric, aggregated or antigen-bound human antibodies in the absence of serum. FDC retain fluorescein-labelled heat-aggregated human immunoglobulins, but not monomeric ones nor fluorescein-labelled F(ab')2 in monomeric or aggregated form. Ultrastructural observations showed that colloidal gold-labelled monomeric, or antigen-bound, antibodies directed against tetanus toxoid are retained by dendrites and membrane infoldings of FDC but are never located in cytoplasmic vesicles. This retention was inhibited by incubating FDC with unlabelled aggregated or antigen-bound antibodies. When gold-labelled anti-tetanus toxoid antibodies were incubated in the presence of protein-A before the contact with FDC, a strong reduction of their retention occurred. This further suggested the presence of Fc receptors on isolated tonsillar FDC. Endocytosis was not observed in isolated FDC, even after prolonged incubation in presence of labelled immune complexes: their Fc receptors are, thus, not related to a phagocytic activity as they are in macrophages. Simultaneous ultrastructural labelling of Fc and C3b receptors with colloidal gold particles of different sizes did not reveal any clear relations between these two receptors on the surface of FDC.

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