Reference : Effects of antimitotic agents either free or bound to DNA on mouse peritoneal macroph...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Effects of antimitotic agents either free or bound to DNA on mouse peritoneal macrophages cultivated in vitro.
Heinen, Ernst mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine >]
Virchows Archiv. B: Cell Pathology
Springer Verlag
Yes (verified by ORBi)
[en] Animals ; Cells, Cultured ; Cisplatin/pharmacology ; DNA/metabolism ; Ethidium/pharmacology ; Macrophages/drug effects/ultrastructure ; Mice ; Mice, Inbred C57BL ; Microscopy, Electron ; Mitosis/drug effects ; Peritoneum/cytology ; Vacuoles
[en] Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell degeneration; cis-Pt was not toxic under our experimental contitions. EB-DNA complex penetrates into the macrophages, is liberated from DNA in vacuoles, then diffuses into the cell and is highly cytotoxic. Cis-Pt-DNA complex also penetrates into the cells, but cis-Pt cannot be released from DNA, cis-Pt-DNA complex accumulates inside cytoplasmic vacuoles but has no cytotoxic activity.

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