Effects of antimitotic agents either free or bound to DNA on mouse peritoneal macrophages cultivated in vitro.

Animals; Cells, Cultured; Cisplatin/pharmacology; DNA/metabolism; Ethidium/pharmacology; Macrophages/drug effects/ultrastructure; Mice; Mice, Inbred C57BL; Microscopy, Electron; Mitosis/drug effects; Peritoneum/cytology; Vacuoles

Abstract :

[en] Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell degeneration; cis-Pt was not toxic under our experimental contitions. EB-DNA complex penetrates into the macrophages, is liberated from DNA in vacuoles, then diffuses into the cell and is highly cytotoxic. Cis-Pt-DNA complex also penetrates into the cells, but cis-Pt cannot be released from DNA, cis-Pt-DNA complex accumulates inside cytoplasmic vacuoles but has no cytotoxic activity.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine

Language :

English

Title :

Effects of antimitotic agents either free or bound to DNA on mouse peritoneal macrophages cultivated in vitro.

Publication date :

1978

Journal title :

Virchows Archiv. B: Cell Pathology

ISSN :

0340-6075

Publisher :

Springer Verlag, Berlin, Germany

Volume :

Issue :

Pages :

79-87

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 22 September 2009

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