Epithelial and endothelial expression of the green fluorescent protein reporter gene under the control of bovine prion protein (PrP) gene regulatory sequences in transgenic mice.
[en] The expression of the cellular form of the prion protein (PrP(c)) gene is required for prion replication and neuroinvasion in transmissible spongiform encephalopathies. The identification of the cell types expressing PrP(c) is necessary to understanding how the agent replicates and spreads from peripheral sites to the central nervous system. To determine the nature of the cell types expressing PrP(c), a green fluorescent protein reporter gene was expressed in transgenic mice under the control of 6.9 kb of the bovine PrP gene regulatory sequences. It was shown that the bovine PrP gene is expressed as two populations of mRNA differing by alternative splicing of one 115-bp 5' untranslated exon in 17 different bovine tissues. The analysis of transgenic mice showed reporter gene expression in some cells that have been identified as expressing PrP, such as cerebellar Purkinje cells, lymphocytes, and keratinocytes. In addition, expression of green fluorescent protein was observed in the plexus of the enteric nervous system and in a restricted subset of cells not yet clearly identified as expressing PrP: the epithelial cells of the thymic medullary and the endothelial cells of both the mucosal capillaries of the intestine and the renal capillaries. These data provide valuable information on the distribution of PrP(c) at the cellular level and argue for roles of the epithelial and endothelial cells in the spread of infection from the periphery to the brain. Moreover, the transgenic mice described in this paper provide a model that will allow for the study of the transcriptional activity of the PrP gene promoter in response to scrapie infection.
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Cesbron, J. Y.
Language :
English
Title :
Epithelial and endothelial expression of the green fluorescent protein reporter gene under the control of bovine prion protein (PrP) gene regulatory sequences in transgenic mice.
Publication date :
2000
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
ISSN :
0027-8424
eISSN :
1091-6490
Publisher :
National Academy of Sciences, Washington, United States - District of Columbia
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Prusiner, S. B. (1998) Proc. Natl. Acad. Sci. USA 95, 13363-13383.
Prusiner, S. B., Scott, M., Foster, D., Pan, K. M., Groth, D., Mirenda, C., Torchia, M., Yang, S. L., Serban, D., Carlson, G. A., et al. (1990) Cell 63, 673-686.
Pan, K. M., Baldwin, M., Nguyen, J., Gasset, M., Serban, A., Groth, D., Mehlhorn, I., Huang, Z., Fletterick, R. J., Cohen, F. E., et al. (1993) Proc. Natl. Acad. Sci. USA 90, 10962-10966.
Caughey, B. W., Dong, A., Bhat, K. S., Ernst, D., Hayes, S. F. & Caughey, W. S. (1991) Biochemistry 30, 7672-7680.
Bolton, D. C., McKinley, M. P. & Prusiner, S. B. (1982) Science 218, 1309-1311.
Diringer, H., Hilmert, H., Simon, D., Werner, E. & Ehlers, B. (1983) Eur. J. Biochem. 134, 555-560.
McKinley, M. P., Bolton, D. C. & Prusiner, S. B. (1983) Cell 35, 57-62.
Prusiner, S. B., Groth, D. F., Bolton, D. C., Kent, S. B. & Hood, L. E. (1984) Cell 38, 127-134.
Gabizon, R., McKinley, M. P., Groth, D. F., Kenaga, L. & Prusiner, S. B. (1988) J. Biol. Chem. 263, 4950-4955.
Prusiner, S. B. (1991) Science 252, 1515-1522.
Bueler, H., Aguzzi, A., Sailer, A., Greiner, R. A., Autenried, P., Aguet, M. & Weissmann, C. (1993) Cell 73, 1339-1347.
Prusiner, S. B., Groth, D., Serban, A., Koehler, R., Foster, D., Torchia, M., Burton, D., Yang, S. L. & DeArmond, S. J. (1993) Proc. Natl. Acad. Sci. USA 90, 10608-10612.
Brown, H. R., Goller, N. L., Rudelli, R. D., Merz, G. S., Wolfe, G. C., Wisniewski, H. M. & Robakis, N. K. (1990) Acta Neuropathol. 80, 1-6.
Bendheim, P. E., Brown, H. R., Rudelli, R. D., Scala, L. J., Goller, N. L., Wen, G. Y., Kascsak, R. J., Cashman, N. R. & Bolton, D. C. (1992) Neurology 42, 149-156.
Horiuchi, M., Yamazaki, N., Ikeda, T., Ishiguro, N. & Shinagawa, M. (1995) J. Gen. Virol. 76, 2583-2587.
DeArmond, S. J., Mobley, W. C., DeMott, D. L., Barry, R. A., Beckstead, J. H. & Prusiner, S. B. (1987) Neurology 37, 1271-1280.
Sales, N., Rodolfo, K., Hassig, R., Faucheux, B., Di Giamberardino, L. & Moya, K. L. (1998) Eur. J. Neurosci. 10, 2464-2471.
Oesch, B., Westaway, D., Walchli, M., McKinley, M. P., Kent, S. B., Aebersold, R., Barry, R. A., Tempst, P., Teplow, D. B., Hood, L. E., et al. (1985) Cell 40, 735-746.
Robakis, N. K., Sawh, P. R., Wolfe, G. C., Rubenstein, R., Carp, R. I. & Innis, M. A. (1986) Proc. Natl. Acad. Sci. USA 83, 6377-6381.
Manson, J., West, J. D., Thomson, V., McBride, P., Kaufman, M. H. & Hope, J. (1992) Development 115, 117-122.
Tanji, K., Saeki, K., Matsumoto, Y., Takeda, M., Hirasawa, K., Doi, K., Matsumoto, Y. & Onodera, T. (1995) Intervirology 38, 309-315.
Moser, M., Colello, R. J., Pott, U. & Oesch, B. (1995) Neuron 14, 509-517.
Mabbott, N. A., Brown, K. L., Manson, J. & Bruce, M. E. (1997) Immunology 92, 161-165.
Cashman, N. R., Loertscher, R., Nalbantoglu, J., Shaw, I., Kascsak, R. J., Bolton, D. C. & Bendheim, P. E. (1990) Cell 61, 185-192.
McBride, P. A., Eikelenboom, P., Kraal, G., Fraser, H. & Bruce, M. E. (1992) J. Pathol. 168, 413-418.
Inoue, S., Tanaka, M., Horiuchi, M., Ishiguro, N. & Shinagawa, M. (1997) J. Vet. Med. Sci. 59, 175-183.
Hogan, B., Beddington, R., Costantini, F. & Lacy, E. (1986) Manipulating the Mouse Embryo: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), 2nd Ed.
Krasemann, S., Groschup, M. H., Harmeyer, S., Hunsmann, G. & Bodemer, W. (1996) Mol. Med. 2, 725-734.
Yoshimoto, J., Iinuma, T., Ishiguro, N., Horiuchi, M., Imamura, M. & Shinagawa, M. (1992) Virus Genes 6, 343-356.
Horiuchi, M., Ishiguro, N., Nagasawa, H., Toyoda, Y. & Shinagawa, M. (1997) Biochem. Biophys. Res. Commun. 233, 650-654.
Saeki, K., Matsumoto, Y., Matsumoto, Y. & Onodera, T. (1996) Biochem. Biophys. Res. Commun. 219, 47-52.
Baybutt, H. & Manson, J. (1997) Gene 184, 125-131.
Fournier, J., Escaig-Haye, F., Billette de Villemeur, T., Robain, O., Lasmezas, C. I., Deslys, J. P., Dormont, D. & Brown, P. (1998) Cell Tissue Res. 292, 77-84.
Harris, D. A., Lele, P. & Snider, W. D. (1993) Proc. Natl. Acad. Sci. USA 90, 4309-4313.
Sachs, A. B. (1993) Cell 74, 413-421.
Brawerman, G. (1987) Cell 48, 5-6.
Sarkozi, E., Askanas, V. & Engel, W. K. (1994) Am. J. Pathol. 145, 1280-1284.
Pammer, J., Weninger, W. & Tschachler, E. (1998) Am. J. Pathol. 153, 1353-1358.
Shmerling, D., Hegyi, I., Fischer, M., Blattler, T., Brandner, S., Gotz, J., Rulicke, T., Flechsig, E., Cozzio, A., von Mering, C., et al. (1998) Cell 93, 203-214.
Fischer, M., Rulicke, T., Raeber, A., Sailer, A., Moser, M., Oesch, B., Brandner, S., Aguzzi, A. & Weissmann, C. (1996) EMBO J. 15, 1255-1264.
Eklund, C. M., Kennedy, R. C. & Hadlow, W. J. (1967) J. Infect. Dis. 117, 15-22.
Fraser, H. & Dickinson, A. G. (1978) J. Comp. Pathol. 88, 563-573.
Muramoto, T., Kitamoto, T., Tateishi, J. & Goto, I. (1992) Am. J. Pathol. 140, 1411-1420.
Kitamoto, T., Muramoto, T., Mohri, S., Doh-Ura, K. & Tateishi, J. (1991) J. Virol. 65, 6292-6295.
Klein, M. A., Frigg, R., Flechsig, E., Raeber, A. J., Kalinke, U., Bluethmann, H., Bootz, F., Suter, M., Zinkernagel, R. M. & Aguzzi, A. (1997) Nature (London) 390, 687-690.
Raeber, A. J., Klein, M. A., Frigg, R., Flechsig, E., Aguzzi, A. & Weissmann, C. (1999) EMBO J. 18, 2702-2706.
Bons, N., Mestre-Frances, N., Belli, P., Cathala, F., Gajdusek, D. C. & Brown, P. (1999) Proc. Natl. Acad. Sci. USA 96, 4046-4051.
van Keulen, L. J., Schreuder, B. E., Vromans, M. E., Langeveld, J. P. & Smits, M. A. (1999) J. Comp. Pathol. 121, 55-63.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.