Reference : Photo-crosslinkable hydrogel for guided periodontal tissue regeneration
Scientific congresses and symposiums : Unpublished conference/Abstract
Engineering, computing & technology : Materials science & engineering
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/224578
Photo-crosslinkable hydrogel for guided periodontal tissue regeneration
English
Chichiricco, Pauline Marie mailto [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France > > >]
Riva, Raphaël [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > > > >]
Thomassin, Jean-Michel [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > > > >]
Struillou, Xavier [University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France > > > >]
Le Visage, Catherine [University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France > > > >]
Weiss, Pierre [University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France > > > >]
Jérôme, Christine mailto [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > > > >]
29-May-2018
No
No
National
Belgian Polymer Group (BPG) Annual Meeting
28/05/2018- 29/05/2018
Blankenberge
Belgium
[en] biomaterial ; scaffold
[en] Periodontitis is an inflammatory disease which destroys the tooth-supporting attachment apparatus. Guided Tissue Regeneration (GTR) is a technique based on a barrier membrane designed to prevent the wound space colonization by gingival cells. Indeed, these cells, characterized by a faster migration and proliferation rate compared to bone and periodontal ligament cells, could interfere with the regeneration process. In this work, we developed a liquid formulation composed of a mixture of silanized hydroxypropylmethyl cellulose (Si-HPMC) and methacrylated carboxymethyl chitosan (MA-CMCS) able to be photochemically crosslinked in situ leading to hydrogel membrane made of an interpenetrated polymer network (IPN) with a synergistic combination of favorable properties of each polymer network. Si-HPMC was selected to assess the barrier effect against soft tissue gingival invasion and CMCS to control the membrane degradation due to its degradable backbone. The methacrylate grafted chains on CMCS allows a photo-curing in short time using visible light lamp (λ 420-480 nm) already used in dentistry and when vitamin B2 (or riboflavin) is used as photoinitiator. The gel point of the IPN precursor formulation was assessed by rheological measurement under lamp irradiation. Mechanical properties after photocrosslinking were studied. Hydrogel membrane degradation was evaluated by soaking hydrogels in 10 mg/ml lysozyme buffer at 37 °C, which confirmed that the addition of CMCS modified the degradation profile of Si-HPMC hydrogel, as evidenced by the recorded weight loss. The in vitro cytocompatibility was evaluated by neutral red uptake using murine fibroblast (L929) in accordance to ISO 10993-5 specifications. Finally, we studied the barrier effect by culturing human primary gingival fibroblasts or human gingival explants on top of the membrane. Cell infiltration was analyzed with confocal microscopy and histological staining (hematoxylin, eosin Y and safranin (HES)). In the next future, in vivo experiment will be performed to further characterize the material for periodontal regeneration.
Complex and Entangled Systems from Atoms to Materials (CESAM) ; Center for Education and Research on Macromolecules (CERM)
The International Doctoral School Nanofar
Researchers
http://hdl.handle.net/2268/224578
This oral communication was presented by Pauline Marie Chichiriccò, and received the price of the best oral presentation

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