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Photo-crosslinkable hydrogel for guided periodontal tissue regeneration
Chichiricco, Pauline Marie; Riva, Raphaël; Thomassin, Jean-Michel et al.
2018Belgian Polymer Group (BPG) Annual Meeting
 

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Keywords :
biomaterial; scaffold
Abstract :
[en] Periodontitis is an inflammatory disease which destroys the tooth-supporting attachment apparatus. Guided Tissue Regeneration (GTR) is a technique based on a barrier membrane designed to prevent the wound space colonization by gingival cells. Indeed, these cells, characterized by a faster migration and proliferation rate compared to bone and periodontal ligament cells, could interfere with the regeneration process. In this work, we developed a liquid formulation composed of a mixture of silanized hydroxypropylmethyl cellulose (Si-HPMC) and methacrylated carboxymethyl chitosan (MA-CMCS) able to be photochemically crosslinked in situ leading to hydrogel membrane made of an interpenetrated polymer network (IPN) with a synergistic combination of favorable properties of each polymer network. Si-HPMC was selected to assess the barrier effect against soft tissue gingival invasion and CMCS to control the membrane degradation due to its degradable backbone. The methacrylate grafted chains on CMCS allows a photo-curing in short time using visible light lamp (λ 420-480 nm) already used in dentistry and when vitamin B2 (or riboflavin) is used as photoinitiator. The gel point of the IPN precursor formulation was assessed by rheological measurement under lamp irradiation. Mechanical properties after photocrosslinking were studied. Hydrogel membrane degradation was evaluated by soaking hydrogels in 10 mg/ml lysozyme buffer at 37 °C, which confirmed that the addition of CMCS modified the degradation profile of Si-HPMC hydrogel, as evidenced by the recorded weight loss. The in vitro cytocompatibility was evaluated by neutral red uptake using murine fibroblast (L929) in accordance to ISO 10993-5 specifications. Finally, we studied the barrier effect by culturing human primary gingival fibroblasts or human gingival explants on top of the membrane. Cell infiltration was analyzed with confocal microscopy and histological staining (hematoxylin, eosin Y and safranin (HES)). In the next future, in vivo experiment will be performed to further characterize the material for periodontal regeneration.
Research center :
CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering
Chemistry
Author, co-author :
Chichiricco, Pauline Marie ;  University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France
Riva, Raphaël ;  University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Thomassin, Jean-Michel ;  University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Struillou, Xavier;  University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France
Le Visage, Catherine;  University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France
Weiss, Pierre;  University of Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, UFR d'Odontologie, France
Jérôme, Christine  ;  University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Language :
English
Title :
Photo-crosslinkable hydrogel for guided periodontal tissue regeneration
Publication date :
29 May 2018
Event name :
Belgian Polymer Group (BPG) Annual Meeting
Event place :
Blankenberge, Belgium
Event date :
28/05/2018- 29/05/2018
Funders :
The International Doctoral School Nanofar
Commentary :
This oral communication was presented by Pauline Marie Chichiriccò, and received the price of the best oral presentation
Available on ORBi :
since 07 June 2018

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