Reference : IL-4Ra-dependent macrophage responses in the liver during murine schistosomiasis
Scientific congresses and symposiums : Poster
Life sciences : Veterinary medicine & animal health
IL-4Ra-dependent macrophage responses in the liver during murine schistosomiasis
Rolot, Marion mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Dougall, Annette [> >]
Gillet, Laurent [> >]
Brombacher, Frank [> >]
Vanderplasschen, Alain mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Dewals, Benjamin G mailto [Université de Liège - ULiège > > Immunologie et vaccinologie >]
47th Annual Meeting of the German Society for Immunology
du 12 septembre 2017 au 15 septembre 2017
German Society for Immunology
[en] Schistosomiasis ; Alternatively activated macrophages ; Liver
[en] During Schistosoma mansoni infection in mice, IL-4 receptor-dependent alternatively activated macrophages (aaMφ) response mediate host protection. Here, we used LysMcreIl4ra−/lox mice to determine whether the absence of Mφ-specific IL-4 receptor signalling modifies the dynamics of Mφ responses in liver granulomas after S. mansoni infection. Liver inflammatory response was investigated after low-dose S. mansoni infection. We observed significantly increased total leukocyte numbers in the liver by week 8 in LysMcreIl4ra−/lox mice while mice survived the infection similarly to littermate controls. Over the course of infection, we observed that CD11blowMerTK+CD64+ resident Küpffer cells (KCs) were severely reducing in term of numbers independently of IL-4Rα signalling. While KCs lowered, Ly6Chi monocytes were recruited in the liver from 6 weeks pi, proliferate strongly at week 8 and acquire CD64 expression. The number of Ly6Chi recruited monocytes were significantly higher in LysMcreIl4ra−/lox mice than littermate controls. From 8 weeks pi, CD11blow KCs seemed to be replaced by CD11bhighMerTK+CD64+F4/80+ Mφ, probably derived from recruited Ly6Chi monocytes. At week 8 pi, expression of Ym1 and Relm-alpha was significantly reduced in hepatic CD11bhigh Mφ of LysMcreIl4ra−/lox mice compared to littermate controls. These results suggest that recruited monocytes differentiate into Mφ at the cost of resident KCs independently of IL-4Rα and validate the use of LysMcreIl4ra−/lox mice to study IL-4Rα-dependent activation of monocyte-derived granuloma Mφ during schistosomiasis.
Fundamental and Applied Research for Animals and Health - FARAH
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Définir les mécanismes d'immunoprotection développés par les macrophages alternativement activés lors de la schistosomiase

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