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UHPLC-ESI-MS/MS quantitation of natural estrogens in aqueous matrices as pyridine-3-sulfonyl derivatives
Glineur, Alex; Ronkart, Sébastien; Nott, Kathérine et al.
2017HPLC 2017
 

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Keywords :
Aqueous matrices; Derivatization; Estrogenic compounds; Pyridine-3-sulfonyl; UHPLC-ESI-MS/MS
Abstract :
[en] Natural estrogens (estrone: E1, 17-β-estradiol : E2, estriol : E3) and synthetic estrogen (17-α-ethinylestradiol: EE2) are powerful endocrine disruptors. They may have deleterious effects on aquatic wildlife and also humans even at extremely low concentrations. For this reason, these molecules have been included in a watch list from the European Commission regarding emerging aquatic pollutants. The maximum detection limits are set at 0.035 and 0.40 ng/L for EE2 and E1, E2 respectively. Reaching such low levels of concentration of estrogenic compounds is a challenge, even using state-of-the-art analytical methods. In this study, we developed a UHPLC-ESI-MS/MS method allowing the quantification of E1, E2, E3 and EE2 residues in aqueous matrices. Studies commonly used ESI in negative mode albeit the poor ionization efficiency of native estrogenic compounds in this mode. In this study, the molecules were derivatised using a sulfonyl chloride reagent (pyridine-3-sulfonyl, P-3-S). The resulting response in the positive mode was significantly enhanced. Similarly to other dansyl derivatives, the product ion spectra of the P-3-S derivatives indicate ions originating from the derivatization reagent moiety. Moreover, several other ions were included in the product ion spectra of the P-3-S derivatives. Their specificity was assessed by a qualitative approach implying the analysis of different types of water samples (groundwater, surface water). Some product ions were found to be noticeably better for quantification and confirmation of the analytes. The developed analytical method was validated according to the NF T90-210 norm which is suitable to assess the performances of a method in the water quality field. The limits of quantification were 0.04, 0.05, 0.10 and 0.02 ng/L in groundwater and 0.89, 0.79,4.42 and 0.29 ng/L in surface water for E1, E2, E3 and EE2 respectively.
Disciplines :
Chemistry
Author, co-author :
Glineur, Alex ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Analyse, qual. et risques - Labo. de Chimie analytique
Ronkart, Sébastien;  Société Wallonne des Eaux - SWDE
Nott, Kathérine;  Société Wallonne des Eaux - SWDE
Carbonnelle, Philippe;  Société Wallonne des Eaux - SWDE
Lognay, Georges ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Analyse, qual. et risques - Labo. de Chimie analytique
Tyteca, Eva ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Analyse, qual. et risques - Labo. de Chimie analytique
Language :
English
Title :
UHPLC-ESI-MS/MS quantitation of natural estrogens in aqueous matrices as pyridine-3-sulfonyl derivatives
Publication date :
22 June 2017
Event name :
HPLC 2017
Event date :
from 18-06-2017 to 22-06-2017
Audience :
International
Available on ORBi :
since 30 March 2017

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