No full text
Article (Scientific journals)
Tilapia prolactin: molecular cloning of two cDNAs and expression in Escherichia coli
Rentier-Delrue, Françoise; Swennen, D.; Prunet, P. et al.
1989In DNA, 8 (4), p. 261-70
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA/genetics/isolation & purification; Escherichia coli/*genetics; Fishes/genetics; Humans; Mammals/genetics; Molecular Sequence Data; Plasmids; Prolactin/*genetics; Protein Sorting Signals/genetics; Restriction Mapping; Salmonidae/*genetics; Sequence Homology, Nucleic Acid; Species Specificity; Trout/*genetics
Abstract :
[en] We have isolated cDNA clones encoding tilapia prolactin (tiPRL) from a cDNA library prepared from tilapia (Oreochromis niloticus) anterior pituitary glands. A trout PRL cDNA fragment was used as hybridization probe to select the recombinant plasmids carrying the tiPRL coding sequence. Two types of PRL cDNA were isolated and their complete nucleotide sequence determined. The larger cDNA (tiPRL-I) codes for a polypeptide of 212 amino acids, including a putative signal sequence of 24 amino acids, and contains a 3' untranslated region of 787 bp. The second prolactin cDNA (tiPRL-II) encodes a polypeptide of 200 amino acids, including a presumptive signal peptide of 23 amino acids, and contains a noncoding region of 512 bp. tiPRL-I and tiPRL-II cDNA sequences are 81% similar, whereas the encoded proteins share 69% amino acid identity at optimal alignment. Mature tiPRL-I was efficiently expressed in Escherichia coli carrying a plasmid in which the tiPRL-I cDNA was under the control of the phi 10 promoter of T7 bacteriophage. The new recombinant protein representing about 45% of the total cellular proteins was found in inclusion bodies and cross-reacted with salmon PRL antiserum.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Rentier-Delrue, Françoise ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique
Swennen, D.
Prunet, P.
Lion, M.
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Tilapia prolactin: molecular cloning of two cDNAs and expression in Escherichia coli
Publication date :
1989
Journal title :
DNA
ISSN :
0198-0238
Publisher :
Mary Ann Liebert, Inc., New York, United States - New York
Volume :
8
Issue :
4
Pages :
261-70
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 August 2009

Statistics


Number of views
72 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
76
Scopus citations®
without self-citations
65
OpenCitations
 
57

Bibliography


Similar publications



Contact ORBi