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Rainbow trout prolactin cDNA cloning in Escherichia coli
Mercier, L.; Rentier-Delrue, Françoise; Swennen, D. et al.
1989In DNA, 8 (2), p. 119-25
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Keywords :
Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; *Dna; Escherichia coli/metabolism; *Genes; Molecular Sequence Data; Prolactin/*genetics/metabolism; RNA, Messenger/*analysis; Salmonidae/*genetics; Sequence Homology, Nucleic Acid; Trout/*genetics
Abstract :
[en] We describe the isolation and characterization of a cDNA for trout prolactin (tPrl). An extensive analysis of tPrl recombinant clones by restriction analysis and sequencing revealed the presence of only one form of tPrl mRNA. The deduced protein sequence consists of 210 amino acids, including a signal peptide of 23 amino acids. The amino acid sequence of the mature protein is compared among teleosts and mammals, showing two domains of strong similarity that may be involved in biological activity.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mercier, L.
Rentier-Delrue, Françoise ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique
Swennen, D.
Lion, M.
Le Goff, P.
Prunet, P.
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Rainbow trout prolactin cDNA cloning in Escherichia coli
Publication date :
1989
Journal title :
DNA
ISSN :
0198-0238
Publisher :
Mary Ann Liebert, Inc., New York, United States - New York
Volume :
8
Issue :
2
Pages :
119-25
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 August 2009

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