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Spectroscopic investigation of structure in octarellin (a de novo protein designed to adopt the alpha/beta-barrel packing)
Beauregard, Marc; Goraj, Karine; Goffin, Vincent et al.
1991In Protein Engineering, 4 (7), p. 745-9
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Keywords :
Circular Dichroism; Fourier Analysis; *Protein Conformation; Protein Denaturation; *Protein Engineering; Recombinant Proteins/*chemistry; Spectrophotometry, Infrared; Spectrum Analysis; Spectrum Analysis, Raman
Abstract :
[en] We present here a spectroscopic structural characterization of octarellin, a recently reported de novo protein modelled on alpha/beta-barrel proteins [K. Goraj, A. Renard and J.A. Martial (1990) Protein Engng, 3, 259-266]. Infrared and Raman spectra analyses of octarellin's secondary structure reveal the expected percentage of alpha-helices (30%) and a higher beta-sheet content (40%) than predicted from the design. When the Raman spectra obtained with octarellin and native triosephosphate isomerase (a natural alpha/beta-barrel) are compared, similar percentages of secondary structures are found. Thermal denaturation of octarellin monitored by CD confirms that its secondary structures are quite stable, whereas its native-like tertiary fold is not. Tyrosine residues, predicted to be partially hidden from solvent, are actually exposed as revealed by Raman and UV absorption spectra. We conclude that the attempted alpha/beta-barrel conformation in octarellin may be loosely packed. The criteria used to design octarellin are discussed and improvements suggested.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Beauregard, Marc
Goraj, Karine
Goffin, Vincent
Heremans, Karel;  Katholieke Universiteit Leuven
Goormaghtigh, Eric
Ruysschaert, Jean-Marie
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Spectroscopic investigation of structure in octarellin (a de novo protein designed to adopt the alpha/beta-barrel packing)
Publication date :
1991
Journal title :
Protein Engineering
ISSN :
0269-2139
eISSN :
1460-213X
Publisher :
Oxford University Press, Oxford, United Kingdom
Volume :
4
Issue :
7
Pages :
745-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 August 2009

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