Temporal pattern of nerve growth factor (NGF) binding in vivo and the in vitro effects of NGF on cultures of developing auditory and vestibular neurons.
Lefèbvre, Philippe; Van de Water, T. R.; Represa, J.et al.
1991 • In Acta Oto-Laryngologica, 111 (2), p. 304-11
[en] NGF binding patterns reflect the presence of receptors for this growth factor. High specific binding of 125I 2.5 S-NGF was observed for the 11 gestation day (gd) statoacoustic ganglion (SAG) with lower levels recorded for both 14 gd acoustic ganglion (AG) and vestibular ganglion (VG) samples. Fourteen day AG cells were more than twice as active for binding NGF when compared to VG samples of the same gestational age. Both whole ganglion explants and dissociated cell cultures were grown in chemically defined medium for short term culture to assay changes in neurite outgrowth and survival of neurons in response to the addition of exogenous 2.5 S-NGF. The most vigorous neurite outgrowth and neuronal survival responses were produced by 11 gd SAG samples treated with NGF. Acoustic ganglion specimens of both 11 gd and 14 gd embryos were much more responsive to the neurotrophic effects of NGF when compared to the responses of their VG counterparts. There was a correlation between NGF binding ability and in vitro responsiveness to exogenous NGF. We hypothesize based on the results of this study that NGF (and/or a member of the NGF family of growth factors) is involved in the control of developmentally regulated neuronal cell death of SAG neurons and may play a role in the innervation of developing inner ear sensory structures.
Disciplines :
Otolaryngology
Author, co-author :
Lefèbvre, Philippe ; Université de Liège > Département des sciences cliniques > Oto-rhino-laryngologie et audiophonologie
Van de Water, T. R.
Represa, J.
Liu, W.
Bernd, P.
Modlin, S.
Moonen, Gustave ; Université de Liège > Département des sciences cliniques > Département des sciences cliniques
Mayer, M. B.
Language :
English
Title :
Temporal pattern of nerve growth factor (NGF) binding in vivo and the in vitro effects of NGF on cultures of developing auditory and vestibular neurons.
Ard M.D., Morest D.K. (1984) Cell death during development of the cochlear and vestibular ganglia of the chick. Int J Dev Neurosci 2:535-547.
Davies A. (1988) The emerging generality of the neurotrophic hypothesis. TINS 11:243-244.
Van De Water T.R., Ruben R.J. (1984) Neurotrophic interactions during in vitro development of the inner ear. Ann Otol Rhinol Laryngol 93:558-564.
Ard M.D., Morest D.K., Hauger S.H. (1985) Trophic interactions between the cochleovestibular ganglion of the chick embryo and its synaptic targets in culture. Neurosci 16:151-170.
Zhou X.N., Van De Water T.R. (1987) The effects of target tissues on survival and differentiation of mammalian statoacoustic ganglion neurons in organ culture. Acta Otolaryngol (Stockh) 104:90-98.
Lefebvre P.P., Leprince P., Weber T., Rigo J.J., Delree P., Moonen G. (1990) Neurotrophic effect of developing otic vesicle on cochleovestibular neurons: Evidence for nerve growth factor involvement. Brain Res 507:254-260.
Bernd P., Represa J. (1989) Characterization and localization of nerve growth factor receptors in the embryonic otic vesicle and cochleovestibular ganglion. Dev Biol 134:11-20.
Theiler K. The house mouse: Development and normal stages from fertilization to 4 weeks of age., Springer-Verlag, Berlin; 1972.
Mobley W.C., Schenker A., Shooter E.M. (1976) Characterization and isolation of proteolytically modified nerve growth factor. Biochemistry 15:5543-5552.
Levi-Montalcini R., Meyer H., Hamburger V. (1954) In vitro experiments on the effects of mouse sarcomas 180 and 37 on the spinal and sympathetic ganglia of the chick embryo. Cancer Res 14:49-57.
Maisonpierre P.C., Bellusci L., Squinto S., Ip N.Y., Furth M.E., Lindsay R.M., Yancopoulos G.D. (1990) Neurotrophin-3: A neurotrophic factor related to NGF and BDNF. Science 247:1446-1451.
Ernfors P., Ibanez C.F., Ebendal T., Olson L., Persson H. (1990) Molecular cloning and neurotrophic activities of a protein with structural similarities to nerve growth factor: Developmental and topographical expression in the brain. Proc Natl Acad Sci (USA) 87:1-4.
Rodriguez-Tebar A., Dechant G., Barde Y.A. (1990) Binding of brain derived neurotrophic factor to the nerve growth factor receptor. Neuron 4:487-492.
Davis A.M., Lindsay R.M. (1985) The cranial sensory ganglia in culture: Differences in the response of placode-derived and neural crest-derived neurons to nerve growth factor. Dev Biol 111:62-72.
Edwards R.H., Rutter W.J., Hanahan D. (1989) Directed expression of NGF to pancreatic B cells in transgenic mice leads to selective hyperinnervation of the islets. Cell 58:161-170.
Davies A.M., Bandtlow C., Heumann R., Korsching S., Rohrer H., Thoenen H. (1987) Timing and site of nerve growth factor synthesis in developing skin in relation to innervation and expression of the receptor. Nature 326:353-357.
Raivich G.J., Zimmermann A., Sutter A. (1987) Nerve growth factor (NGF) receptor expression in chicken cranial development. J Comp Neurol 256:229-245.
Hallbook F., Ayer-Lelievre C., Ebendal T., Persson H. (1990) Expression of nerve growth factor receptor mRNA during early development of the chicken embryo: Emphasis on cranial ganglia. Development 108:693-704.
Represa J., Bernd P. (1989) Nerve growth factor and serum differentially regulate development of the embryonic otic vesicle and cochleovestibular ganglion in vitro. Dev Biol 134:21-29.
Depres G., Giry N., Romand R. (1988) Immunohistochemical localization of nerve growth factor-like protein in the organ of Corti of the developing rat. Neurosci Lett 85:5-8.