No full text
Paper published in a book (Scientific congresses and symposiums)
Molecular characterization of double minutes/homogeneously staining regions in selected tumor cell lines
Conteduca, Giuseppina; Trombetta, Domenico; Storlazzi, Tiziana C et al.
2008In Trombetta, Domenico; Lonoce, Angelo; Guastadisegni, Maria C et al. (Eds.) the official journal of the International Society for Cellular Oncology
 

Files


Full Text
No document available.

Send to



Details



Keywords :
double minutes; MycN; Oncogene
Abstract :
[en] Double minutes (dmin) and homogeneously staining regions (hsr) are the cytogenetic hallmarks of genomic amplification in cancer. Different mechanisms have been proposed to explain their genesis. Recently, our group showed that the MYC-containing dmin in leukemia cases arise by excision and amplification (episome model). In the present paper we investigated 10 cell lines from solid tumors showing MYCN amplification as dmin or hsr. Particularly revealing results were provided by the two subclones of the neuroblastoma cell line STA-NB-10, one showing dmin-only and the second hsr-only amplification. Both subclones showed a deletion, at 2p24.3, whose extension matched the amplicon extension. Additionally, the amplicon structure of the dmin and hsr forms was identical. This strongly argues that the episome model, already demonstrated in leukemias, applies to solid tumors as well, and that dmin and hsr are two faces of the same coin. The organization of the duplicated segments varied from very simple (no apparent changes from the normal sequence) to very complex. MYCN was always overexpressed (significantly overexpressed in three cases). The fusion junctions, always mediated by nonhomologous end joining, occasionally juxtaposed truncated genes in the same transcriptional orientation. Fusion transcripts involving NBAS (also known as NAG), FAM49A, BC035112 (also known as NCRNA00276), and SMC6 genes were indeed detected, although their role in the context of the tumor is not clear.
Research center :
University of Bari, Italy
Disciplines :
Genetics & genetic processes
Author, co-author :
Conteduca, Giuseppina ;  Université de Liège > GIGA-R : Hématologie
Trombetta, Domenico;  University of Bari, Italy > genetics and microbiology
Storlazzi, Tiziana C;  University of Bari, Italy > genetics and microbiology
Surace, Cecilia;  Hopital Bambin Gesù, Rome (IT) > genetics
Language :
English
Title :
Molecular characterization of double minutes/homogeneously staining regions in selected tumor cell lines
Publication date :
04 January 2008
Event name :
2nd Marie Curie-Genome Architecture in Relation to Disease Meeting
Event organizer :
Spain
Event date :
du 4 Mai 2008 au 7 Mai 2008
Audience :
International
Main work title :
the official journal of the International Society for Cellular Oncology
Author, co-author :
Trombetta, Domenico
Lonoce, Angelo
Guastadisegni, Maria C
Surace, Cecilia
Conteduca, Giuseppina ;  Université de Liège - ULiège > Département des sciences cliniques > GIGA-R : Hématologie
Daniele, Giulia
Kok, klaas
Ullmann, Reinhard
Vlies Van der, Pieter
Buys, Charles
Rocchi, Mariano
Storlazzi, Tiziana C
Collection name :
30
Available on ORBi :
since 07 July 2016

Statistics


Number of views
23 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Bibliography


Similar publications



Contact ORBi