[en] Pituitary GH3 cells were transfected with different deletion mutants of the human prolactin (hPRL) promoter fused to the CAT reporter gene. The proximal region (-250 to -42) was sufficient to confer stimulation by both thyrotropin-releasing hormone (TRH) and epidermal growth factor (EGF). Further deletion analyses demonstrated the importance of the three proximal Pit-1 binding sites in this response. However, Pit-1 binding oligonucleotides confer neither TRH nor EGF induction to a linked neutral promoter, suggesting that other elements might be involved. We have previously shown that sequence A (-115 to -85) is needed together with Pit-1 binding sites for full cyclic AMP response of hPRL-CAT. Mutation of this sequence strongly affects TRH and EGF induction. On the other hand, three copies of sequence A confer both TRH and EGF response to a linked neutral promoter. In conclusion, although TRH and EGF activate mostly different intracellular pathways, they mediate transcriptional induction of the hPRL promoter via identical cis elements.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Berwaer, Monique; Université de Liège - ULiège
Peers, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Nalda, Asuncion M; Université de Liège - ULiège
Monget, Philippe; Université de Liège - ULiège
Davis, Julian R; University of Manchester
Belayew, Alexandra; Université de Liège - ULiège
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Thyrotropin-releasing hormone and epidermal growth factor induce human prolactin expression via identical multiple cis elements
Publication date :
1993
Journal title :
Molecular and Cellular Endocrinology
ISSN :
0303-7207
eISSN :
1872-8057
Publisher :
North Holland Publishing Company, Amsterdam, Netherlands
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Bibliography
Berridge, Heslop, Irvine, Brown Biochem. J. 1984, 222:195-201.
Gorman, Moffat, Howard Mol. Cell. Biol. 1982, 2:1044-1051.
Higuchi, Krummel, Saiki (1988) A general method ofin vitropreparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Research 16:7351-7360.
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