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DNA metabolism and development of organelles in guinea-pig megakaryocytes: a combined ultrastructural, autoradiographic and cytophotometric study
Paulus, Jean-Michel
1970 • In Blood, 35 (3), p. 298-311
Peer Reviewed verified by ORBi
Permalink
https://hdl.handle.net/2268/191972
DOI
10.1182/blood.v35.3.298.298
PubMed
5437680
 

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Keywords :
Hemopoiesis; Megakaryocytes; Polyploidy
Abstract :
[en] The thick and thin section technique was used to combine ultrastructural studies of guinea-pig megakaryocytes with either autoradiographic studies of thymidine incorporation into megakaryoblast DNA or cytophotometric determination of megakaryocyte ploidy. Megakaryocytes engaged in polyploidization already showed granule and some demarcation membrane formation. The polyploidization phase stopped at the 8N, 16N, or 32N level after 3 plus or minus 0.5 (1 SD) genome duplications.
Research Center/Unit :
Institut de Pathologie Cellulaire, Hopital de Bicêtre, 94-Le Kremlin-Bicêtre, France
Disciplines :
Hematology
Author, co-author :
Paulus, Jean-Michel ;  Université de Liège > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
DNA metabolism and development of organelles in guinea-pig megakaryocytes: a combined ultrastructural, autoradiographic and cytophotometric study
Publication date :
March 1970
Journal title :
Blood
ISSN :
0006-4971
eISSN :
1528-0020
Publisher :
Amer Soc Hematology
Volume :
35
Issue :
3
Pages :
298-311
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Mechanism of thrombocytopoiesis
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Institut de Pathologie Cellulaire, Hopital de Bicêtre, 94-Le Kremlin-Bicêtre, France
Donner Laboratory, University of California, Berkeley, Calif, USA
Available on ORBi :
since 22 January 2016

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