The Certification Of The Identity Of Individual Sterols In 3 Bcr Oil And Fat Reference Materials By Gc-Ms

The sterols of three Reference Materials: a vegetable oil (RM162: a mixture of Soya oil and Maize oil) and two animal fats (RM163: a blend of Pig and Beef fats and RM164: Milkfat) were identified by GC-MS in two experienced laboratories. The techniques used were practically the same nevertheless, Lab. 1 and 2 used respectively an HPLC and a classical TLC method to isolate the sterols from the unsaponifiable matter. Many minor components were detectable when the high efficient HPLC fractionation technique was used but the only molecules similarly identified by the two Labs were considered for the certification of sterol identities. In this article, we present the results of the certification study together with the interpretation of mass spectra.