[en] The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70). The isolated insert-a BamHI 5.6-kb fragment-was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Brans, Alain ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Loriaux, Axelle; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Dusart, Jean; Université de Liège - ULiège > Centre d'Ingénierie des Protéines
Language :
English
Title :
Cloning and Sequencing of the Dnak Locus in Streptomyces Coelicolor A3(2)
Publication date :
1996
Journal title :
DNA Sequence: the Journal of DNA Sequencing and Mapping
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