Reference : Age-related Macular Degeneration Study: A Metabolomics Approach
Scientific congresses and symposiums : Unpublished conference/Abstract
Human health sciences : Ophthalmology
Age-related Macular Degeneration Study: A Metabolomics Approach
LAMBERT, Vincent mailto [Centre Hospitalier Universitaire de Liège - CHU > > Ophtalmologie >]
De Tullio, Pascal mailto [Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique >]
Hansen, Sylvain mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
GIGA Cancer Seminar
du 04/10/2013 au 04/10/2013
[en] Age-Related Macular degeneration ; NMR ; AMD
[en] Age-related macular degeneration (AMD) is a leading cause of vision loss in the western world among people aged 50 or older. 90% of all vision loss due to AMD result from the exudative form, which is characterized by choroidal neovascularization (CNV). Age-related changes that induce pathologic CNV are incompletely understood. A successful application of anti-VEGF approaches in the clinic is obviously a turning point in AMD treatment. Nevertheless, despite such important advances, critical issues remain to be addressed. To better understand the etiology of this pathology, we have used and improved a model of laser-induced murine choroidal neovascularization. As none is known about the metabolic changes in patients with AMD, we decided to apply a 1H NMR metabolomics approach on AMD patients and on a mice CNV experimental model.
This technique is a relatively new branch of « omics » technologies focused on the analysis and measurement of endogenous metabolites. These experiments provide unique challenges to fulfill the goal of improving the current status of physiological information related to metabolome and in general to functional genomics.
For this purpose, sera from control and AMD patients, induced and non-induced mice have been collected and the metabolic profiles of these samples were determined by 1H NMR. After post-processing treatments, the different spectra were analyzed by statistical discriminant methodologies (PCA, ICA, PLS-DA).
This approach allows the differentiation between control and AMD patients and between laser-induced mice and the control mice group. Moreover, the same discriminating spectral zones, lactate and lipoproteins profil, have been identified in human and mice model, leading to the emergence of different putative biomarkers. In mice model of laser-induced CNV, normalization of circulating lactate by dichloroacetate, decreases CNV development. These first interesting results open new way in the study of the AMD etiology and validate the use of 1H NMR and CNV model for the understanding of Age-related Macular Degeneration.
Researchers ; Students

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