Reference : Iron content in human alveolar macrophages.
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
Iron content in human alveolar macrophages.
CORHAY, Jean-Louis mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie]
Weber, Georges mailto [Université de Liège - ULiège > > Centre européen en archéométrie]
Bury, Thierry mailto [Université de Liège - ULiège > Département des sciences de la motricité > Physiologie humaine et physiologie de l'effort physique]
Mariz, S [> > > >]
Roelandts, I [> > > >]
Radermecker, Maurice mailto [Université de Liège - ULiège > > Relations académiques et scientifiques (Médecine) >]
European Respiratory Journal
European Respiratory Society
Yes (verified by ORBi)
[en] Anti-Glomerular Basement Membrane Disease/diagnosis ; Bronchoalveolar Lavage Fluid/pathology ; Ferrocyanides ; Humans ; Iron/analysis ; Lung Diseases, Obstructive/diagnosis ; Lung Neoplasms/diagnosis ; Macrophages, Alveolar/chemistry ; Middle Aged ; Occupational Exposure ; Siderosis/diagnosis ; Smoking/physiopathology ; Spectrometry, X-Ray Emission ; Staining and Labeling
[en] Intracellular iron can be estimated semi-quantitatively by histochemical determination using the ferrocyanide reagent's score. Particle-induced X-ray emission (PIXE) allows accurate determination of various elements including iron in cells and biological fluids. Both techniques have been used to measure iron in alveolar macrophages gathered by bronchoalveolar lavage. The purpose of this study was to investigate the clinical usefulness of the PIXE technique in occupational respiratory medicine and in various pulmonary diseases. Using the PIXE method, we measured the iron content of alveolar macrophages in healthy subjects, with and without occupational exposure to iron dust, and in patients with pulmonary diseases (chronic obstructive pulmonary disease (COPD), lung cancer, Goodpasture's syndrome). Our results were then compared with those obtained with the ferrocyanide reagent. Intramacrophagic iron was 0.33 +/- 0.21 micrograms.10(-6) (mean +/- SD) cells in healthy non-smoking subjects without occupational exposure. Intramacrophagic iron was increased in smokers, iron-steelworkers, and in patients with COPD or lung cancer even in the absence of pulmonary haemorrhage. The two patients with Goodpasture's syndrome had high intramacrophagic iron content. About 80% of the whole bronchoalveolar lavage fluid iron content was in the cells. Mean iron content of blood monocytes, lymphocytes and neutrophils of eight healthy subjects was significantly lower than that of alveolar macrophages. A significant correlation was found between iron determination by the PIXE method and the ferrocyanide reagent's score (r = 0.89). We conclude that intramacrophagic iron may be increased in steelworkers and subjects with pulmonary haemorrhage, but also in asymptomatic smokers, in COPD and lung cancer patients without occupational exposure to iron dust.

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