[en] Steroid 16 alpha-hydroxylase activities and properties were studied in C57Bl/6J, 129/J, AKR/R, DBA/2J, C3H/I, and BALB/c mouse liver using four different substrates. The highest enzymatic activities were measured in the female mice, with the exception of the 129/J females. As in the rat liver, the sexual differentiation of the steroid 16 alpha-hydroxylation observed in adult male and female mice took place at puberty. In the adult mouse liver, two steroid 16 alpha-hydroxylase activities (forms I and II) could be differentiated on the basis of their relative affinities for the various steroid substrates and their relative proportions in male and female mouse livers. In the immature mouse liver, no sexual differences could be detected, and the mice of both sexes presented phenotypes identical to those of the adult female. The adult 129/J females appeared genetically deficient with respect to the form I of the steroid 16 alpha-hydroxylase and presented a phenotype identical to that of the adult male mice of the various strains tested. Differences in hydroxylase activities between the C57Bl/6J and 129/J strains were investigated using standard genetic breeding protocols. Steroid 16 alpha-hydroxylase seemed to be inherited additively in the liver of the female mice obtained by crossing the C57Bl/6J male and the 129/J female or the 129/J male and the C57Bl/6J female. In the male mice, regardless of genotype, the observed phenotype was always identical to the two male parental types. Both hormonal and genetic regulations were responsible for the different phenotypes occurring in adult male and female C57Bl/6J and 129/J mouse livers.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pasleau, Françoise ; Université de Liège - ULiège > CARE "Le Réseau des bibliothèques" > Bibliothèque des Sciences de la vie
Kolodzici, Claudine
Kremers, Pierre ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Gielen, Jacques
Language :
English
Title :
Genetic regulation of hepatic steroid 16 alpha-hydroxylase activities in inbred strains of mice.
Publication date :
October 1984
Journal title :
Endocrinology
ISSN :
0013-7227
eISSN :
1945-7170
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Hamberg M, Samuelsson B, Bjòrkhem I, Danielsson H 1974 Oxy-génases in fatty acid and steroid metabolism. Molecular Mechanisms of Oxygen Activation. Academic Press, New York, p 33
Pasleau F, Kremers P, Gielen JE 1980 Sex differences in the activity of different cytochrome P-450 dependent steroid 16α-hydroxylases in rat liver. J Steroid Biòchem 13: 733
Pasleau F, Kremers P, Gielen JE 1981 Competition between benzo(a)pyrene and various steroids for cytochrome P-450 dependent rat liver monooxygenases. Chem Biol Interact 33: 279
Pasleau F, Kolodzici C, Kremers P, Gielen JE 1981 Ontogenic development of steroid 16α-hydroxylase as a tool for the study of the multiplicity of cytochrome P-450. Eur J Biochem 120: 213
Ford HC, Eddington L, Engel LL 1979 Circannual variation and genetic regulation of hepatic testosterone hydroxylase activities in inbred strains of mice. Endocrinology 104: 857
Kremers P., De Graeve J, Azhir A, Gielen JE 1978 Measurement of testosterone- and dehydroepiandrosterone-16α-hydrbxylase activities by a tritium exchange method. Eur J Biochem 82: 529
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ 1951 Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265
Omura T, Sato R 1964 The carbon monoxide binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 239: 2970
De Graeve J, Kremers P, Gielen JE 1977 Measurement of progesterone-and pregnenolone-16α-hydroxylase activities by a tritium exchange method. Eur J Biochem 74: 561
Van Cantfort J, De Graeve J, Gielen JE 1977 Radioactive assay for aryl hydrocarbon hydroxylase. Improved method and biological importance. Biochem Biophys Res Commun 79: 505
Kremers P, Pasleau F, Gielen JE 1978 Is steroid 16α-hydroxylase supported by more than one monooxygenase? Biochem Biophys Res Commun 84: 706
Gustafsson JA, Eneroth P, Poussette A, Skett P, Sonnenschein C, Steberg A, Ahlen A 1977 Programming and differentiation of rat liver enzymes. J Steroid Biochem 8: 429
Chung LWK 1977 Characteristics of neonatal androgens induced imprinting of rat hepatic microsomal monooxygenases. Biochem Pharmacol 26: 1979
Lamartiniere CA, Dieringer CS, Kita E, Lucier GW 1979 Altered sexual differentiation of hepatic uridine diphosphate glucuronyl-transferase by neonatal hormone treatment in rats. Biochem J 180: 313
Van den Berg AP, Noordhoek J, Savenije-Chapel EM, Koopman-Kool F 1978 The development of sex differences in the demethyl-ation of ethylmorphine and in its interaction with components of the hepatic microsomal cytochrome P-450 system in mice. Biochem Pharmacol 27: 627
Gustafsson J-Å, Mode A, Norstedt G, Skett P 1983 Sex steroid induced changes in hepatic enzymes. Annu Rev Physiol 45: 51
Mode A, Norstedt G, Simic B, Eneroth P, Gustafsson J-Å 1981 Continuous infusion of growth hormone feminizes hepatic steroid metabolism in the rát. Endocrinology 108: 2103
Terry LC, Saunders A, Audet J, Willoughby J, Brazeau P, Martin JB 1977 Physiologic secretion of growth hormone and prolactin in male and female rats. Clin Endocrinol (Oxf) [Suppl] 6: 19s
Edén S 1979 Age- and sex-related differences in episodic growth hormone secretion in the rat. Endocrinology 105: 555
Mode A, Gustafsson J-Å, Jánsson JO, Edén S, Isaksson O 1982 Association between plasma level of growth hormone and sex differentiation of hepatic steroid metabolism in the rat. Endocrinology 111: 1692