[en] A new method for continuous blood collection using the Biostator is described. Blood is withdrawn through the double lumen catheter by a tube installed in the optional channel of the infusion pump. The amount of blood withdrawn from the patient is slightly greater than that necessary for continuous glucose analysis; the excess blood can be collected into assay tubes. Blood collection is continuous and produces a sample of diluted heparinized blood. The volume of blood collected depends on the size of the tube used, i.e. for a tube with a lumen diameter of 0.020 inches, the mean (+/- SD) volume collected was 1.21 +/- 0.07 ml/10 min (n = 13). The mean time interval between sampling and arrival at the glucose sensor by the double lumen catheter was 119 versus 108 s with the conventional method. The proposed modification does not affect blood glucose measurements (correlation coefficient compared with the reference method r = 0.9572; n = 13). To compensate for blood dilution, a dilution-factor depending on tubing diameter has to be calculated in each experiment.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Castillo, M. J.
Scheen, André ; Université de Liège - ULiège > Département des sciences cliniques > Diabétologie, nutrition et maladie métaboliques - Médecine interne générale
Luyckx, A. S.
Lefebvre, Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Diabétologie,nutrition, maladies métaboliques
Language :
English
Title :
Blood collection while using a continuous glucose analyzer without insertion of an additional venous catheter.
Verdonk C.A., Rizza R.A., Westland R.E., Nelson R.L., Gerich J.E., Service F.J. (1980) Glucose clamping using the Biostator GCIIS. Horm Metab Res 12:133-135.
Home P.D., Massi-Benedetti M., Shepherd G.A.A., Hanning I., Alberti K.G.M.M., Owens D.R. (1982) A comparison of the activity and disposal of semi-synthetic human insulin and porcine insulin in normal man by the glucose clamp technique. Diabetologia 22:41-45.
Valenta L.J., Elias A.N., Grossman M., Tanner S.M., Friis R. (1982) A new test of peripheral insulin sensitivity in vivo using artificial beta cell. Life Sciences 30:867-873.