Reference : Transcriptional activation capacity of the novel PLAG family of zinc finger proteins.
Scientific journals : Article
Life sciences : Genetics & genetic processes
Transcriptional activation capacity of the novel PLAG family of zinc finger proteins.
Kas, K. [> > > >]
Voz, Marianne mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Hensen, K. [> > > >]
Meyen, E. [> > > >]
Van de Ven, W. J. [> > > >]
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
Yes (verified by ORBi)
[en] Amino Acid Sequence ; Animals ; COS Cells ; Cell Cycle Proteins ; Cloning, Molecular ; Conserved Sequence ; DNA Mutational Analysis ; DNA, Complementary/genetics ; DNA-Binding Proteins/genetics ; Genes, Reporter ; Genes, Tumor Suppressor ; Humans ; Molecular Sequence Data ; Multigene Family ; RNA-Binding Proteins/genetics ; Saccharomyces cerevisiae/genetics ; Sequence Analysis, DNA ; Sequence Deletion ; Sequence Homology, Amino Acid ; Trans-Activators/genetics ; Transcription Factors ; Transcriptional Activation ; Tumor Suppressor Proteins ; Zinc Fingers/genetics
[en] We have isolated and characterized two novel cDNAs encoding C2H2 zinc finger proteins showing high sequence homology to PLAG1, a protein ectopically activated by promoter swapping or promoter substitution in pleomorphic adenomas with chromosomal abnormalities at chromosome 8q12. PLAG1 and the two new PLAG1 family members (PLAGL1 and PLAGL2) constitute a novel subfamily of zinc finger proteins that recognize DNA and/or RNA. To examine the potential of the three human proteins to modulate transcription, we constructed several PLAG/GAL4 DNA binding domain fusion proteins and measured their ability to activate transcription of a reporter gene construct in different mammalian cell lines and in yeast. Although the carboxyl-terminal part of PLAGL1 shows strong overall transcriptional activity in mesenchymal (COS-1) and epithelial cells (293), both PLAG1 and PLAGL2 transactivate in mesenchymal cells only if depleted from a repressing region. This effect is less profound in epithelial cells. These data suggest that the activation in pleomorphic adenomas of PLAG1 most likely results in uncontrolled activation of downstream target genes.

There is no file associated with this reference.

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.