[en] This study was designed to compare the effects of three prolonged exercises varying in their intensity and duration, on blood mononuclear cell mobilization and cytokine secretion (IL1(1)-IL(2)). Seven healthy subjects underwent three effort trials (45 % VO(2)max during 4 h - 60% VO(2)max during 3 h - 75 % VO(2)max during 2 h) at one-month intervals. Blood samples were drawn before, different times during exercise and also after exercise. Prolonged exercises induced a transient increase in blood mononuclear cells which occurred across all intensity levels. We also observed a significant increase in plasma IL(1) level during exercise which correlates with the exercise intensity. The mean IL(1) level increased up to 2.5 times after the three proposed exercises (p <0.05). Plasma IL(2) level decreased at the end of prolonged exercises irrespective of the exercise intensity. No correlation was observed between blood mononuclear count and cytokine determination. Our data suggest that blood mononuclear cells mobilization is associated but not correlated with alterations of cytokine levels.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Bury, Thierry ; Université de Liège - ULiège > Département des sciences de la motricité > Physiologie humaine et physiologie de l'effort physique
Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Bieger W. P., Weiss M., Michel G., Weicker H.: Exercise-induced monocytosis and modulation of monocyte function. Int J Sports Med 1: 30-36, 1980.
Bollinger P. B., Drewinko B., Brailas C., Smeeton N., Trujillo J.: The technicon H1 - an automated haematology analyser for today and tomorrow. Am J Clin Pathol 87: 71-78, 1987.
Cannon J. G., Kluger M. J.: Endogenous pyrogen activity in human plasma after exercise. Science 220: 617-619, 1983.
Cannon J. G., Evans W. J., Hughes V. A., Meredith C. N., Dinarello C. A.: Physiological mechanisms contributing to increased interleukin-1 secretion. J Appl Physiol 61: 1869-1874, 1986.
De Groote D., Zangerle P., Gevaert Y., Fassotte M., Beguin Y., Noizat-Pirenne F.: Direct stimulation of cytokines (IL-1β, TNFα, IL6, IL2, INFγ and GM-CSF) in whole blood. Comparison with isolated PBMC stimulation. Cytokine 4: 239-248, 1992.
Deuster P. A., Curiale A. M., Cowan M. L., Finkelman F. D.: Exercise-induced changes in populations of peripheral blood mononuclear cells. Med Sci Sports Exerc 20: 276-280, 1988.
Dill D., Costill D.: Calculation of percentage changes in volumes of blood, plasma and red cells in dehydration. J Appl Physiol 37: 247-248, 1974.
Espersen G. T., Elback A., Ernst E., Toft E., Kaalund S., Jersild C., Grunnet N.: Effect of physical exercise on cytokines and lymphocyte subpopulations in human peripheral blood. APMIS 98: 395-400, 1990.
Evans W. J., Meredith C. N., Cannon J. G., Dinarello C. A., Frontera W. R., Hughest V. A., Jones B. H., Knuttgen H. G.: Metabolic changes following eccentric exercise in trained and untrained men. J Appl Physiol 61: 1864-1868, 1986.
Fitzgerald L.: Exercise and the immune system. Immunol Today 9: 337-339, 1988.
Galbo H.: Endocrinology and metabolism in exercise. Int J Sports Med 2: 203-211, 1981.
Galund E., Burstein R., Assia E., Tur Kaspa I, Rosenblum J., Epstein Y.: Changes of white blood cell count during prolonged exercise. Int J Sports Med 8: 253-255, 1987.
Hoffman-Goetz L., Simpson J. R., Cipp N., Arumugan Y., Houston M. E.: Lymphocyte subset responses to repeated submaximal exercise in men. J Appl Physiol 68: 1069-1074, 1990.
Keast D., Cameron K., Morton A. R.: Exercise and the immune response. Sports Med 5: 248-267, 1988.
Lewicki R., Tchorzewski H., Denys A., Kowalska M., Golinska A.: Effect of physical exercise on some parameters of immunity in conditioned sportsmen. Int J Sports Med 8: 309-314, 1987.
Lewicki., Tchorzewski H., Majewska E., Nowak Z., Baj Z.: Effect of maximal physical exercise on T-lymphocyte subpopulations and on interleukin 1 (IL 1) and interleukin 2 (IL 2) production in vitro. Int J Sports Med 9: 114-117, 1988.
Mac Carthy D., Dale M.: The leucocytosis of exercise. Sports Med 6: 333-363, 1988.
Mac Kinnon L. T.: Exercise and natural killer cells. What is the relationship? Sports Med 1: 141-149, 1989.
Nieman D., Berk L, Simpson-Westerberg M., Arabatzis K., Youngberg S., Tan S., Lee J., Eby W.: Effects of long-endurance running on immune system parameters and lymphocyte function in experienced marathoners. Int J Sports Med 10: 317-323, 1989.
Northoff H., Berg A.: Immunological mediators as parameters of the reaction to strenuous exercise. Int J Sports Med 12: S9-S15, 1991.
Oshida Y., Yamanouchi K., Kayamizu Y., Sato Y.: Effect of acute physical exercise on lymphocyte subpopulations in trained and untrained subjects. Int J Sports Med 9: 137-140, 1988.
Pedersen B. K., Tvede N., Hansen F. R., Andersen V., Bendix T., Bendixen G., Bendtzen K., Galbo H., Haahr P. M., Klarlund K., Sylvest J., Thomsen B. S., Halkjaer-Kristensen J.: Modulation of natural killer cell activity in peripheral blood by physical exercise. Scand J Immunol 27: 673-678, 1988.
Rupp E., Cameron P., Ramawat C.: The specific bioactivities of monocyte derived interleukin-1α and interleukin-1β are similar to each other on cultured murine thymocytes and cultured human connective tissue cells. J Clin Invest 78: 836-839, 1986.
Sprenger H., Jacobs C., Nain M., Gressner A. M., Prinz H., Wesemann W., Gemsa D.: Enhanced release of cytokines, interleukin-2 receptors and neopterin after long-distance running. Clin Immunol Immunopathol 63: 188-195, 1992.
Viru A.: Plasma-hormone and physical exercise. Int J Sports Med 13: 201-209, 1992.
Viti A., Muscettola M., Paulesu L., Bocci V., Almi A.: Effect of exercise on plasma interferon levels. J Appl Physiol 59: 426-428, 1985.
Waldman T.: The structure, function and expression of IL2 receptors on normal and malignant lymphocytes. Science 232: 727-732, 1986.
Zerbe G.: Randomization analysis of randomized blocks design extended to growth and response curves. Commun Stat A8: 191-205, 1979.