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Mechanochemical Study of Conformational Transitions in a Single Synthetic Peptide Chain
Willet, Nicolas; Hinterdorfer, Peter; Lecommandoux, Sébastien et al.
2012Gordon Research Conference: Biopolymers
 

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Keywords :
AFM; Single-molecule; Peptide; Folding; Conformation
Abstract :
[en] The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in detail by atomic force microscopy (AFM) at the single-molecule level. Synthetic copolymers containing a polypeptide block were prepared by N-carboxyanhydride amino acid ring-opening polymerization. The polymer chains were grafted as a dilute brush onto gold surfaces via disulfide end-groups. Their mechanochemical behavior was then studied by AFM single-molecule force spectroscopy (SMFS). The investigated polypeptide blocks were based on poly(L-glutamic acid), which undergoes a transition from alpha-helix to random coil. This can be induced by external stimuli (pH, ionic strength, temperature) or simply by applying a force. We were able to study the mechanically driven unfolding of the peptide by stretching-release cycles of the biomacromolecule. Stretching the helical peptide resulted in original features in the force-distance traces. Plateaus that are specific for the helical conformation were detected, quantified and discussed. Pulling-relaxing SMFS experiments eventually led to a better understanding of the force induced unfolding of an alpha-helix and the reversibility of the phenomenon.
Research center :
NanoChem
Disciplines :
Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Physics
Chemistry
Author, co-author :
Willet, Nicolas ;  Université de Liège - ULiège > Département de chimie (sciences) > Nano-chimie et systèmes moléculaires
Hinterdorfer, Peter;  Johannes Kepler University Linz > Biophysics Institute
Lecommandoux, Sébastien;  Université Bordeaux 1 > LCPO
Duwez, Anne-Sophie  ;  Université de Liège - ULiège > Département de chimie (sciences) > Nano-chimie et systèmes moléculaires
Language :
English
Title :
Mechanochemical Study of Conformational Transitions in a Single Synthetic Peptide Chain
Publication date :
05 June 2012
Event name :
Gordon Research Conference: Biopolymers
Event organizer :
GRC
Angel E. Garcia
Enrique De La Cruz
Event place :
Newport, United States - Rhode Island
Event date :
June 3-8, 2012
Audience :
International
Available on ORBi :
since 25 June 2012

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