[en] BACKGROUND: Obese patients are frequently characterized by insulin resistance and decreased insulin-mediated glycogen synthesis in skeletal muscle. Whether they also have impaired postprandial hepatic glycogen synthesis remains unknown. AIM: To determine whether postprandial hepatic glycogen synthesis is decreased in obese patients compared to lean subjects. METHODS: Lean and obese subjects with impaired glucose tolerance were studied over 4h after ingestion of a glucose load. Hepatic uridine diphosphoglucose kinetics were assessed using 13C-galactose infusion, with monitoring of urinary acetaminophen-glucuronide isotopic enrichment to estimate hepatic glycogen kinetics. RESULTS: Estimated net hepatic glycogen synthesis amounted to 18.6 and 22.6% of the ingested load in lean and obese subjects, respectively. CONCLUSION: Postprandial hepatic glycogen metabolism is not impaired in non-diabetic obese subjects.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Paquot, Nicolas ; Centre Hospitalier Universitaire de Liège - CHU > Diabétologie,nutrition, maladies métaboliques
Schneiter, P.
Scheen, André ; Université de Liège - ULiège > Département des sciences cliniques > Diabétologie, nutrition et maladie métaboliques - Médecine interne générale
Lefebvre, Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Diabétologie,nutrition, maladies métaboliques
Tappy, L.
Language :
English
Title :
Assessment of postprandial hepatic glycogen synthesis from uridine diphosphoglucose kinetics in obese and lean non-diabetic subjects.
Lefebvre P.J., Scheen A.J. (1999) Glucose metabolism and the postprandial state. Eur J Clin Invest 29(SUPPL. 2):1-6.
Gerich J.E. (1993) Control of glycaemia. Bailliere's Clin Endocrinol Metab 7:551-586.
Tappy L. (1995) Regulation of hepatic glucose production in healthy subjects and in NIDDM. Diabetes Metab 21:233-240.
Rebrin K., Steil G.M., Getty L., Bergman R.N. (1995) Free fatty acid as a link in the regulation of hepatic glucose output by peripheral insulin. Diabetes 44:1038-1045.
Stalmans W., Bollen M., Mvumbi L. (1987) Control of glycogen synthesis in health and disease. Diabetes Metab Rev 3:127-161.
Tappy L., Dussoix P., Iynedjian P., Henry S., Schneiter P., Zahnd G., Jequier E., Philippe J. (1997) Abnormal regulation of hepatic glucose output in maturity onset diabetes of the young caused by a specific mutation of the glucokinase gene. Diabetes 46:204-208.
Cherrington A.D. (1999) Control of glucose uptake and release by the liver in vivo. Diabetes 48:1198-1214.
Mitrakou A., Kelley D., Veneman T., Jenssen T., Pangburn T., Reilly J., Gerich J. (1990) Contribution of abnormal muscle and liver glucose metabolism to postprandial hyperglycemia in NIDDM. Diabetes 39:1381-1390.
Mitrakou A., Kelley D., Mokan M., Veneman T., Pangburn T., Reilly J., Gerich J. (1992) Role of reduced suppression of glucose production and diminished early insulin release in impaired glucose tolerance. New Engl J Med 326:22-29.
Felber J.P., Acheson K.J., Tappy L., From Obesity to Diabetes. J Wiley: Chichester; 1992.
Shulman G., Rothman D., Jue T., Stein P., DeFronzo R., Shulman R. (1990) Quantitation of muscle glycogen synthesis in normal subjects and in subjects with non-insulin dependent diabetes by 13C nuclear magnetic resonance spectroscopy. New Engl J Med 322:223-228.
Ludvik B., Nolan J.J., Roberts A., Baloga J., Joyce M., Bell J.M., Olefsky J.M. (1995) A noninvasive method to measure splanchnic glucose uptake after oral glucose administration. J Clin Invest 95:2232-2238.
Hellerstein M.K., Munro H.N. (1988) Glycoconjugates as noninvasive probes of intrahepatic metabolism: III. Application to galactose assimilation by the intact rat. Metabolism 37:312-317.
Durnin J.V.G.A., Womersley J. (1979) Body fat assessment for total body density and its estimation from skinfold thickness: Measurement 481 men and women from 16 to 72 y. Br J Nutr 32:77-97.
Gray D.S., Bray G.A., Bauer M., Kaplan K., Gemayel N., Wood R., Greenway F., Kirk S. (1990) Skinfold thickness measurements in obese subjects. Am J Clin Nutr 51:571-577.
Gay L.J., Schneiter P., Schutz Y., Di Vetta V., Jequier E., Tappy L. (1994) A non invasive assessment of hepatic glycogen kinetics and post-absorptive gluconeogenesis in man. Diabetologia 37:517-523.
Magnusson I., Chandramouli V., Schumann W.C., Kumaran K., Wahren J., Landau B.R. (1988) Pentose pathway in human liver. Proc Natl Acad Sci USA 85:4682-4685.
DeBodo R., Steele R., Altszuler N., Dunn A., Bishop J. (1963) On the hormonal regulation of carbohydrate metabolism: Studies with 14C-glucose. Recent Prog Horm Res 19:445-488.
Hellerstein M.K., Neese R.A., Letscher A., Linfoot P., Turner S. (1997) Hepatic glucose-6-phosphatase flux and glucose phosphorylation, cycling, irreversible disposal, and net balance in vivo in rats. Measurement using the secreted glucuronate technique. Metabolism 46:1390-1398.
(1998) Report of the Expert Committee on the diagnosis and classification of diabetes mellitus. Diabetes Care 21(SUPPL. 1).
Schneiter P., Gillet M., Chiolero R., Jequier E., Tappy L. (1999) Hepatic nonoxidative disposal of an oral glucose meal in patients with liver cirrhosis. Metabolism 48:1260-1266.
Magnusson I., Rothman D.L., Katz L.D., Shulman R.G., Shulman G.I. (1992) Increased rate of gluconeogenesis in type II diabetes mellitus. A 13C nuclear magnetic resonance study. J Clin Invest 90:1323-1327.
Taylor R., Magnusson I., Rothman D.L., Cline G.W., Caumo A., Cobelli C., Shulman G.I. (1996) Direct assessment of liver glycogen storage by 13C nuclear magnetic resonance spectroscopy and regulation of glucose homeostasis after a mixed meal in normal subjects. J Clin Invest 97:126-132.
Cline G.W., Rothman D.L., Magnusson I., Katz L.D., Shulman G.I. (1994) Nuclear magnetic resonance spectroscopy studies of hepatic glucose metabolism in normal subjects and subjects with insulin-dependent diabetes mellitus. J Clin Invest 94:2369-2376.
Scheen A.J., Paquot N., Letiexhe M.R., Castillo M.J., Lefebvre P.J. (1995) Glucose metabolism in obese subjects: Lessons from OGTT, IVGTT and clamp studies. Int J Obes Relat Metab Disord 19(SUPPL. 3).
