Single molecule; Force spectroscopy; Molecular recognition; AFM tip; probe; Functionalization; Recognition imaging
Abstract :
[en] This chapter describes the state of the art in molecular recognition force spectroscopy performed by AFM. The different aspects of the topic are discussed, as the appropriate techniques for the functionalization of cantilever tips and for the preparation of (biological) samples. The principles of single-molecule force spectroscopy are then explained, together with exciting and recent examples on synthetic and biological samples. Finally, the main techniques to map molecular recognition interactions are reviewed and discussed in terms of performances. Novel and interesting applications illustrate the use of these imaging methods.
Disciplines :
Chemistry Life sciences: Multidisciplinary, general & others Biochemistry, biophysics & molecular biology Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others Physics
Author, co-author :
Willet, Nicolas ; Université de Liège - ULiège > Département de chimie (sciences) > Nano-chimie et systèmes moléculaires
Lamprecht, Constanze; Johannes Kepler University of Linz > Biophysics Institute
Rankl, Christian; Agilent Technologies Austria GmbH
Rangl, Martina; Johannes Kepler University of Linz > Biophysics Institute
Creasey, Rhiannon; Flinders University of South Australia > School of Chemical and Physical Sciences
Ebner, Andreas; Johannes Kepler University of Linz > Biophysics Institute
Voelcker, Nicolas H.; Flinders University of South Australia > School of Chemical and Physical Sciences
Hinterdorfer, Peter; Johannes Kepler University of Linz > Biophysics Institute
Language :
English
Title :
Molecular recognition force spectroscopy
Publication date :
2011
Main work title :
Molecular manipulation with atomic force microscopy
Editor :
Duwez, Anne-Sophie ; Université de Liège - ULiège > Molecular Systems (MolSys)
Willet, Nicolas ; Université de Liège - ULiège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires
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