A Sequence-Specific DNA-Binding Protein Interacts with the Xlnc Upstream Region of Streptomyces Sp. Strain Ec3

Giannotta, F.; Georis, J.; Moreau, A.; Mazy-Servais, C.; Joris, Bernard; Dusart, Jean

doi:10.1016/0378-1097(96)00249-2

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Article (Scientific journals)

A Sequence-Specific DNA-Binding Protein Interacts with the Xlnc Upstream Region of Streptomyces Sp. Strain Ec3

Giannotta, F.; Georis, J.; Moreau, A. et al.

1996 • In FEMS Microbiology Letters, 142 (1), p. 91-7

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https://hdl.handle.net/2268/106949

DOI
10.1016/0378-1097(96)00249-2

PubMed
8759794

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Abstract :

[en] The alignment of the promoter region of several Streptomyces xylanases shows three conserved sequences which could be involved in gene regulation. By electromobility shift assays these specific sequences, present only in Streptomyces xylanolytic strains, were identified as protein-binding sites. The sequence required for efficient recognition by the retarding protein appeared to be a 4-bp inverted repeat: 5'-CTTT-Nx-AAAG-3'. The DNA-protein affinity was influenced by the culture conditions.

Disciplines :

Microbiology

Author, co-author :

Giannotta, F.

Georis, J.

Moreau, A.

Mazy-Servais, C.

Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Dusart, Jean

Language :

English

Title :

A Sequence-Specific DNA-Binding Protein Interacts with the Xlnc Upstream Region of Streptomyces Sp. Strain Ec3

Publication date :

15 August 1996

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Oxford University Press, United Kingdom

Volume :

142

Issue :

Pages :

91-7

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 02 January 2012

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Bibliography

[1] Delic, I., Robbins, P. and Westpheling, J. (1992) Direct repeat sequences are implicated in the regulation of two Streptomyces chitinase promoters that are subject to carbon catabolite control. Proc. Natl. Acad. Sci. USA 89, 1885-1889.
[2] Mattern, S.G., Brawner, M.E. and Westpheling, J. (1993) Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon. J. Bacteriol. 175, 1213-1220.
[3] Virolle, M.J. and Gagnat, J. (1994) Sequences involved in growth-phase-dependent expression and glucose repression of a Streptomyces α-amylase gene. Microbiol. 140, 1059-1067.
[4] Hindle, Z. and Smith, C.P. (1994) Substrate induction and catabolite repression of the Streptomyces coelicolor glycerol operon are mediated through the GylR protein. Mol. Microbiol. 12, 737-745.
[5] Malfait, M., Godden, B. and Penninckx, M.J. (1984) Growth and cellulase production of Micromonospora chalcae and Pseudonocardia thermophila. Ann. Microbiol. (Inst. Pasteur) 135B, 69-78.
[6] Mazy-Servais, C., Moreau, A., Gérard, C. and Dusart, J. (1996) Cloning and nucleotide sequence of a xylanase-encoding gene from Streptomyces sp. strain EC3. DNA Sequence 6, 147-158.
[7] Nagashima, M., Okumoto, Y. and Okanishi, M. (1989) Nucleotide sequence of the gene of extracellular xylanase in Streptomyces sp. No. 36a and construction of secretion vectors using xylanase gene. In: Trends in Actinomycetology in Japan (Koyama, Y., Ed.), pp. 91-96. Society for Actinomycetes, Tokyo, Japan.