References of "Poster"
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See detailStudy of virus-like particles of human papillomavirus in capillary electrophoresis
Bettonville, Virginie ULiege; Nicol, Jérôme; Furst, Tania et al

Poster (2017)

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See detailStructural characterization of protein using an enzymatic reactor
Grifnée, Elodie ULiege

Poster (2017)

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See detailFeedback effect on children's global metacognitive judgments
Geurten, Marie ULiege; Meulemans, Thierry ULiege

Poster (2017)

In three experiments, we investigated whether the feedback effect on the accuracy of children’s metacognitive judgments results from an improvement in monitoring processes or the use of the Anchoring-and ... [more ▼]

In three experiments, we investigated whether the feedback effect on the accuracy of children’s metacognitive judgments results from an improvement in monitoring processes or the use of the Anchoring-and-Adjustment heuristic. The purpose of Experiment 1 was to investigate whether 4-, 6-, and 8-year-old children are able to use feedback to increase the accuracy of their memory judgments. To do so, children in three age groups were divided into two experimental conditions (feedback or no feedback). After studying a list of associated words, participants were instructed to predict their future memory performance, and then they were asked to recall as many items as possible. Next, half of the participants were given concrete feedback about the accuracy of their global prediction. Once the feedback was provided, all children were presented with another set of associated word pairs and the procedure was repeated. Our results revealed that children’s predictions were more accurate in the feedback than in the no feedback condition, indicating that getting feedback about the accuracy of their judgments had a positive influence on their subsequent memory predictions. In Experiment 2, we sought to determine whether young children are able to use the Anchoring-and-Adjustment heuristic to guide their global memory predictions. For this purpose, 4-, 6-, and 8-year-old children were divided into three experimental conditions depending on the anchor that was provided (high, low, or no anchor). Data indicated that children’s predictions were higher in the high than in the low anchor condition, suggesting that children in all age groups adjusted their prospective judgment depending on the random anchor they were given. Finally, the primary aim of Experiment 3 was to determine whether the feedback effect can serve as an external anchor for children’s global prospective judgments. To do so, children (aged 4, 6, and 8 years) were presented with two memory tasks that differed in terms of difficulty. Participants were randomly assigned to an anchoring (high, low, or no anchor) and a feedback (feedback or no feedback) condition to obtain a balanced experimental design. Results showed that children in the feedback condition adjusted their predictions toward the feedback, regardless of the task’s difficulty. These findings are consistent with the hypothesis that external information provided by feedback is used as an anchor for judgment. This interpretation is strengthened by the correlation found between the two scores computed to assess participants’ susceptibility to anchoring and feedback effects, which indicates that children who are more sensitive to the anchoring effect are also more sensitive to the feedback effect. [less ▲]

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See detailA legacy survey of early B-stars using the RGS
Nazé, Yaël ULiege; Cazorla, C.; Rauw, Grégor ULiege et al

Poster (2017)

Despite their relatively large incidence among early-type stars, only a few B-stars have been observed with X-ray gratings. We therefore undertook a legacy project with XMM to gather high- resolution ... [more ▼]

Despite their relatively large incidence among early-type stars, only a few B-stars have been observed with X-ray gratings. We therefore undertook a legacy project with XMM to gather high- resolution spectra of a set of early B stars selected on the basis of their high RASS count rate. This poster presents the preliminary results of this project. [less ▲]

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See detailAround the fireplace: heat exposure and adhesive alteration
Cnuts, Dries ULiege; Tomasso, Sonja ULiege; Rots, Veerle ULiege

Poster (2017)

Currently, there is no agreement about the timing of the habitual use of fire in the Palaeolithic. Some researchers (Roebroeks and Villa, 2011) situate the control of fire in North-western Europe around ... [more ▼]

Currently, there is no agreement about the timing of the habitual use of fire in the Palaeolithic. Some researchers (Roebroeks and Villa, 2011) situate the control of fire in North-western Europe around 400ka and question the early claims of fire structures (before 400 ka) since these traces could also be produced by natural events. The only other available line of direct evidence, strike-a-lights, appear only to occur later in the Palaeolithic record (Stapert and Johansen, 1999; Sorensen et al., 2014; Rots, 2015). The lack of convincing evidence has forced researchers to use indirect evidence as adhesives to estimate the timing of control of fire. It is assumed that a synthetic adhesive like birch tar, which was already in use from at least 120ka (Mazza et al., 2006), cannot be produced without an extensive pyro-technological knowledge. The link between fire control and adhesive technology is evident since fire is required for a range of activities related to hafting technology: the production of birch bark, mixing of resin with other materials, dehafting of stone tools. Moreover, archaeological evidence indicates that dehafted stone tools may have been thrown into the fire when discarded. Once the tools are buried, they may be subjected to heat from an overlying fireplace. The effect of heat exposure on these fragile organic substances has never been investigated, and it is hypothesised that this might be a possible explanation for the rare survival of these adhesives in the archaeological record. Our study aims at monitoring the effect of heat exposure by combustion on a range of experimental compound adhesives on flint tools. The results of the combustion experiments are presented and it is demonstrated that the vertically transferred combustion heat is responsible for the loss of adhering adhesives. A correlation between the degree of loss and the specific adhesive mixture could be observed. The combustion experiment also leads to a wide range of accidental residues deposited on the stone tools. Our results stress the importance of identifying the processes that might be responsible for the formation or degradation of residues adhering to a stone tool surface. [less ▲]

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See detailReactive extrusion of pharmaceutical grade PLLA
Regibeau, Nicolas ULiege; Tilkin, Rémi ULiege; Grandfils, Christian ULiege et al

Poster (2017)

During the 20th century, degradable aliphatic polyesters have undergone fast and dynamic developments. Nowadays, these materials can be found in several areas of human activities. Originally there have ... [more ▼]

During the 20th century, degradable aliphatic polyesters have undergone fast and dynamic developments. Nowadays, these materials can be found in several areas of human activities. Originally there have been first designed for the pharmaceutical and medical fields, in particular in surgery and for drug delivery systems. In these domains, polymers are synthesized according to a batch procedure due to the low capacity needed. This work is dedicated to the optimization of a continuous synthesis of pharmaceutical grade polyesters (e.g. poly-L-lactide, PLLA) by reactive extrusion using a twin screws extruder. This equipment presents several advantages compared to batch reactor such as: absence of solvent, high degree of mixing, easiness of scale-up, and rapid continuous synthesis. PLLA synthesis by reactive extrusion has been performed adopting a co-rotating twin-screws extruder (diameter=11 mm and L/D ratio=40). A highly active catalyst must be used to reach the target conversion due to the limited residence time. Tin octoate, approved by US Food and Drugs Administration, has been used alone as catalyst considering a catalyst / monomer molar ratio of 1/5000. A polyethylene glycol has been adopted as initiator. 1H.NMR and size exclusion chromatography (SEC) have been adopted to monitor the conversion rate of the monomer and to analyse the mean molecular weights and the corresponding polydispersity. Static mechanical tests have also carried out to assess the influence of residual monomer within the polymer. Our optimization study has highlighted that the main challenging aspects were the control of the reaction atmosphere and of the residence time. In the first case, even presence in trace water can competitively initiate the polymerization, but also promote hydrolysis. In order to reach a high monomer conversion (≥ 99 %), the residence time has to be well controlled and extended. This latter parameter is particularly affected by the screw configuration and the use of the protic initiator. In particular, the screw configuration was based on previous studies dedicated to the synthesis of polyesters by reactive extrusion [1, 2]. After synthesis, post-processing step was realized in order to reduce the monomer residue. Once optimized we have succeeded to reach in a reproducible way high molecular weight PLLA (typically in a range of 50 to 100 KDa) with a high monomer conversion (>96 %) on a time scale of some minutes. Post-polymerization has successfully decreased the residual monomer below 1%. In conclusion, this work provides a continuous and robust process to synthesize pharmaceutical grade PLLA by reactive extrusion. References : [less ▲]

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See detailRewiring of porcine mRNA and miRNA networks in response to selection of residual feed intake
Beiki, Hamid; Schroyen, Martine ULiege; Rakshandeh, Anoosh et al

Poster (2017)

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See detailDevelopment and integration of entomopathogenic fungi for crops protection in Democratic Republic of Congo
Fingu Mabola, Junior Corneille ULiege; Bawin, Thomas; Nsevolo Miankeba, Papy et al

Poster (2017)

The Democratic Republic of the Congo (DR Congo) has enormous agricultural potentialities likely to contribute to its economic development. However, the sector is characterized by significant yield losses ... [more ▼]

The Democratic Republic of the Congo (DR Congo) has enormous agricultural potentialities likely to contribute to its economic development. However, the sector is characterized by significant yield losses due notably to the pressure of pests. In response to this situation, most farmers use synthetic chemical pesticides, despite the problems of residues on harvested materials and their widely documented side effects on biodiversity. Up to now, efforts are made to promote healthy farming in the long term, taking into account both economic and environmental aspects. According to this principals, pest management is now achieved through the combination of agronomic, biological and physical means, chemical control being the last resort if necessary. Considering the context of DR Congo, biological control remains by far the least developed of all means in pest management. This is due to the lack of technical equipment. Nevertheless, compared to entomophagous macroorganisms, microorganisms offer the advantage of not requiring substantial resources for their integration into agriculture. This is notably the case for entomopathogenic fungi (EF): in addition to the fact that they may be multiplied on readily available substrates, their abundance in nature makes it possible to isolate new, more efficient strains with more or less marked selectivity. However, very little research on EF is carried out in this country. The present study aims to integrate these biological control agents into the pest management strategy in DR Congo. This research project will focus on two approaches: (1) evaluation of the genetic diversity of EF strains in DR Congo, which will lead to the establishment of a reference collection; and (2) screening of potential candidates for biopesticide development. Researches will be carried out on the southwestern part of the country, focusing on insect cadavers and soil samples from cultivated areas. Pure strains will be isolated on synthetic culture media then identified according to their morphological, physiological and molecular traits. Biological tests including virulence and effectiveness under various environmental conditions in laboratory will be carried out against the most important agricultural pests to be determined following a field monitoring. Finally, culture trials of EF with locally available resources at low cost will be conducted to encourage the adoption of the technology. [less ▲]

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See detailEffect of agitation rate on oxydative degradation of monoethanolamine
Benkoussas, Hana ULiege; Léonard, Grégoire ULiege; Kerbachi, Rabah

Poster (2017)

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See detailIncidence of MAGED2 on cell cycle after a camptothecin treatment
Trussart, Charlotte ULiege; Pirlot, Céline; Piette, Jacques ULiege et al

Poster (2017)

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See detailÉtude de la spécificité d’épreuves langagières chez des enfants présentant une dyspraxie verbale (DV) vs un trouble phonologique (TP)
Masson, Pauline ULiege; Martinez Perez, Trecy ULiege

Poster (2017)

Malgré une augmentation de la recherche sur la dyspraxie verbale, l’évaluation de ce trouble langagier reste problématique. La littérature propose une variété de critères pour le diagnostic de la ... [more ▼]

Malgré une augmentation de la recherche sur la dyspraxie verbale, l’évaluation de ce trouble langagier reste problématique. La littérature propose une variété de critères pour le diagnostic de la dyspraxie verbale, cependant la majorité des caractéristiques cliniques sont également partagées avec les troubles phonologiques (Davis & Velleman, 2000 ; Murray et al., 2015). L’objectif de cette recherche est de déterminer des épreuves qui permettent de différencier une dyspraxie verbale et un trouble phonologique. Nous avons exploré les marqueurs les plus couramment utilisés dans la littérature : l’imprécision des productions phonologiques et l’intelligibilité réduite (Aziz et al., 2010), l’inconstance des erreurs (Forrest, 2003 ; Tubul-Lavy, 2012), la prosodie inappropriée (Shriberg et al., 2003) et la lenteur d’articulation (Shriberg, 2013). Nous avons récolté des données auprès de deux groupes d’enfants francophones présentant une dyspraxie verbale (N=12) ou un trouble phonologique (N=12). Nos deux groupes sont appariés sur le score du QI non verbal, le niveau de compréhension orale et le niveau socio-économique. Les résultats de cette recherche mettent en évidence quatre mesures dont les valeurs de sensibilité/spécificité sont entre acceptables (>80%) et bonnes (>90%) : les pourcentages de réponses correctes et de consonnes correctes à une épreuve de dénomination d'images ; la stabilité des productions dans une épreuve de récit sur images (mots présentés à trois reprises) ; et la vitesse d’articulation pour la série diadocosinésique /pataka/. Pour ces quatre mesures, les résultats montrent que la dyspraxie verbale et le trouble phonologique se situent sur un continuum de sévérité (la dyspraxie verbale entrainant des manifestations plus sévères que le trouble phonologique). Ces résultats seront discutés lors de la session poster. [less ▲]

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See detail[18F]UCB-H RADIOTRACER AS A TOOL TO UNDERSTAND THE ROLE OF THE SV2A PROTEIN
Serrano Navacerrada, Maria Elisa ULiege; Becker, Guillaume ULiege; Bahri, Mohamed Ali ULiege et al

Poster (2017)

Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking, being important both in normal as in pathological process (1, 2 ... [more ▼]

Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking, being important both in normal as in pathological process (1, 2). Until now, only one study in vivo has been reported, showing a reduction of SV2A levels in the epilepsy (3). [18F]UCB-H was developed like a current tool to study the role of SV2A with in vivo techniques (4, 5), and as a tool in clinical investigations. The objective of this research was to evaluate the radiotracer specificity to this isoform comparing with the others, through a competition assay in rats with ex-vivo autoradiography and mPET imaging. Methods: Forty male Sprague-Dawley were used in ex-vivo autoradiography experiments (N=20) and in microPET imaging (N=20). Animals were pre-treated 30 minutes before the injection of [18F]UCB-H with a dose IP either of vehicle, Keppra (SV2A ligand), UCB068 (SV2B ligand) or UCB054 (SV2C ligand). Ex-vivo autoradiography was carried out 5 minutes after radiotracer injection while mPET images were acquiring with a dynamic scanner of 1 hour. Standard Uptake Value (SUV) and Distribution Volume (VT) were calculated and the correlation between both parameters was determined. Results: In ex-vivo autoradiography, ANOVA of two-ways showed statistical significant differences in brain uptake of [18F]UCB-H among the groups pretreated with Keppra or the ligand for SV2B and the control group. Regarding mPET data, statistical significant differences were found between the group injected with keppra and the rest of groups. Pearson Correlation between SUV and VT was strong, with a value of 0.955. Conclusion: Even if a considerable affinity between the ligands UCB068 and UCB054, and the receptor for the isoform SV2A exists, it is only detected during the first 5 minutes (ex-vivo technique), being certainly due to a nonspecific binding. This binding is not strong enough to show a direct competition with the radiotracer during a mPET acquisition. These results allow us to conclude that [18F]UCB-H is a suitable radiotracer for the imaging of the isoform SV2A in vivo, allowing us the clinical study about the molecular base of a disease with a high population impact, like the epilepsy. 1) Van Vliet et al., 2009. Epilepsia 2) Crèvecœur et al., 2013. BMC Neurosci. 3) Finnema et al., 2016; Sci Transl Med. 4) Bretin et al., 2013.EJNMMI Res 5) Bretin et al., 2015.Mol Imaging Biol [less ▲]

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See detailContribution of macrophages to myxomatous valve disease
Hulin, Alexia ULiege; Potter, Sarah; Kim, Andrew et al

Poster (2017)

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See detailIdentifying tissue specific gene expression using RNAseq data from multiple porcine tissues
Huang, Jianzhen; Schroyen, Martine ULiege; Gabler, Nick et al

Poster (2017)

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See detailIN VIVO STUDY OF THE SV2A PROTEIN IN AN EPILEPTIC RAT MODEL
Serrano Navacerrada, Maria Elisa ULiege; Becker, Guillaume ULiege; Bahri, Mohamed Ali ULiege et al

Poster (2017)

Introduction Epilepsy is one of the commonest neurological disorders, affecting more than 60 million people worldwide [1]. New and effective antiepileptic drugs mainly target the SV2A protein [2] but its ... [more ▼]

Introduction Epilepsy is one of the commonest neurological disorders, affecting more than 60 million people worldwide [1]. New and effective antiepileptic drugs mainly target the SV2A protein [2] but its actual role is still largely unknown. [18F]UCB-H was developed as a tool to study in vivo the brain expression of this isoform [3, 4]. Due to the fact that only post-mortem studies were reported so far [5] the present pilot study was undertaken in order to evaluate for the first time in vivo in rats the SV2A expression in the validated Kaïnic Acid (KA) epilepsy model [6]. Methods Three male Sprague-Dawley were used, one injected with saline (Sham) and two with multiple KA systemic injections (5mg/kg x 3) [9]. SV2A brain levels were estimated at day 75, when spontaneous seizures started to appear. Animals were anesthetized (2.5 to 3 % isoflurane), and scanned for 1 hour with [18F]UCB-H (41 ± 5 MBq IV tail vein) in a Focus 120 microPET system and with MRI (9.4T Agilent, anatomical T2). Coregistration was done with PMOD 3.6 software. Data were expressed in SUV and areas under the curve were calculated for the different regions. Results [18F]UCB-H microPET images showed an important reduction (20-30%) for SV2A after KA injections mainly localized in amygdala, hippocampus, lateral parietal association cortex and cingulate cortex. The rest of the brain was globally unchanged. MRI revealed atrophy and inflammation in amygdala and hippocampus. Conclusions These preliminary results in KA treated rats presenting spontaneous seizures showed that [18F]UCB-H microPET was able to detect important reductions for the SV2A proteins in relevant regions for epilepsy [5]. Accordingly to this, we can infer that the KA model in rats deserves for further development and validation as a tool for the study of epilepsy. [18F]UCB-H appears as a valuable tool to follow in vivo SV2A proteins through longitudinal protocols and in turn to better understand its actual role in epilepsy. References/acknowledgements This work was funded by University of Liège, F.R.S.-FNRS, Walloon Region and UCB Pharma. Alain Plenevaux is research director from F.R.S.-FNRS. [1] Alexopoulos, Epileptology, 2004 [2] Hamann et al., Eur J Pharmacol, 2008 [3] Bretin et al., Molecular Imaging and Biology, 2015 [4] Warnock et al., J Nucl Med., 2014 [5] Wang et al., J Mol Neurosci., 2014 [6] Hellier et al., Epilepsy Res., 1998 [less ▲]

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See detailIN VIVO STUDY OF THE SV2A PROTEIN IN THE KAINIC ACID EPILEPSY RAT MODEL
Serrano Navacerrada, Maria Elisa ULiege; Becker, Guillaume ULiege; Bahri, Mohamed Ali ULiege et al

Poster (2017)

Introduction Epilepsy is one of the commonest neurological disorders [1]. Antiepileptic drugs mainly target the SV2A protein [2] but its actual role is still largely unknown. [18F]UCB-H was developed to ... [more ▼]

Introduction Epilepsy is one of the commonest neurological disorders [1]. Antiepileptic drugs mainly target the SV2A protein [2] but its actual role is still largely unknown. [18F]UCB-H was developed to study in vivo SV2A brain proteins [3, 4]. The present pilot study was undertaken to evaluate for the first time in vivo in rats SV2A expression in the Kaïnic Acid (KA) epilepsy model [5]. Although this model is well studied in mice, few reports were devoted to rats. Imaging-wise, rats are very interesting thanks to a bigger brain size (reduction of the partial volume effect). Methods Three male Sprague-Dawley were used, one injected with saline and two with multiple KA injections (3 x 5mg/kg) [6]. 75 days later, when spontaneous seizures started to appear, microPET (Focus 120 ) was performed under isoflurane anesthesia (2.5-3 % in air) for 1 hour with [18F]UCB-H (41 ± 5 MBq IV tail vein) followed by MRI (9.4T Agilent, anatomical T2). Coregistration was done with PMOD 3.6 software. Data were expressed as SUV and areas under the curve were calculated for the different regions. Results [18F]UCB-H microPET images showed an important reduction (20-30%) for SV2A after KA injections mainly localized in amygdala, hippocampus, lateral parietal association cortex and cingulate cortex. The rest of the brain was globally unchanged. MRI revealed atrophy and inflammation in amygdala and hippocampus. Conclusions These preliminary results obtained in KA treated rats showed that [18F]UCB-H was able to detect important modifications for SV2A in relevant regions for epilepsy and appears as a valuable tool to follow in vivo SV2A through longitudinal studies. KA model in rats deserves for further development and validation as a tool for the study of epilepsy. [less ▲]

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See detailGalectin-1 decreases during osteoclast formation
Muller, Joséphine ULiege; BINSFELD, Marilène ULiege; DUBOIS, Sophie ULiege et al

Poster (2017)

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See detailEnhancement of the internalization of lipid nanocapsules in human glioblastoma cells: Effect of surface concentration of NFL peptide
Karim, Reatul ULiege; Lepeltier, Elise; Palazzo, Claudio ULiege et al

Poster (2017)

Le glioblastome multiforme (GBM) est un des cancers les plus fatal, avec une médiane de survie de 14 mois après traitement. Il est donc nécessaire de développer de nouvelles thérapies plus efficaces. La ... [more ▼]

Le glioblastome multiforme (GBM) est un des cancers les plus fatal, avec une médiane de survie de 14 mois après traitement. Il est donc nécessaire de développer de nouvelles thérapies plus efficaces. La fonctionnalisation en surface de nanocapsules lipidiques (LNC) avec le peptide NFL-TBS.40-63 (NFL) a déjà montré une amélioration de leur internalisation dans des cellules de glioblastome murin. Le but de cette étude a été d’évaluer l’impact de la concentration en NFL présente en surface des LNC sur l’internalisation de ces dernières dans des cellules humaines de GBM U87MG. De plus, le mécanisme d’internalisation LNC-NFL a été étudié. Une sonde fluorescente (DiA) a été encapsulé dans : des LNC (F1), des LNC avec 0.86 % et 2.58 % (w/w) de NFL adsorbé à la surface (F2 et F3 respectivement). Des analyses par cytométrie en flux (FACS) ont révélé une internalisation cellulaire de F3 plus importante de 46.4 et 6.8 fois après 30 min, de 21.6 et 6.1 fois après 1 heure, de 31.5 et 1.6 fois après 6 heures et de 7.3 et 1.1 fois après 24 heures, comparés à F1 et F2 respectivement. L’internalisation de F3 dans les cellules U87MG s’est révélée être énergie-dépendant, avec comme mécanisme principal la macropinocytose. Les cinétiques de désorption du peptides (obtenues par dialyse de F3 dans du Tris-Buffer pH 7.4 à 37°C suivi par une HPLC analytique) ont montré que 66 % de NFL restaient à la surface des LNC après 6h de dialyse, montrant une désorption lente. De plus, les trois formulations ont montré une faible activation du complément. Du fait d’une internalisation significativement plus prononcée et rapide, F3 semble être prometteur pour améliorer l’efficacité des thérapies antiGBM. De plus, la lente désorption du NFL de la surface des LNC et la faible consommation du complément font de F3 une thérapie ciblé prometteuse contre le GBM. [less ▲]

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See detailPlant Oxylipins: Structure-function Relationships
Genva, Manon ULiege; Andersson, Mats X.; Nasir, Mehmet Nail ULiege et al

Poster (2017)

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See detailPigs selected for increased natural resistance to PRRS are more resistant to PRRSV/PCV2b co-infection
Dunkelberger, Jenelle R; Serão, Nicolas VL; Niederwerder, Megan et al

Poster (2017)

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See detailBehavioural phenotyping of SV2A lox/lox mice: Motor and anxiety-like features
Serrano Navacerrada, Maria Elisa ULiege; Bartholomé, Odile ULiege; Van Den Ackerveken, Priscilla ULiege et al

Poster (2017)

Background: Epilepsy is one of the most common neurological disorders (Alexopoulos, 2004). Current anti-epileptic drugs, such as Levetiracetam (Keppra®) or Brivaracetam, mainly target the trans-membrane ... [more ▼]

Background: Epilepsy is one of the most common neurological disorders (Alexopoulos, 2004). Current anti-epileptic drugs, such as Levetiracetam (Keppra®) or Brivaracetam, mainly target the trans-membrane Synaptic Vesicle Protein 2A (Hamann et al., 2008). Studies on homozygous SV2A KO mice phenotype, prove the mice to suffer severe seizures and die within 3 weeks (Crowder et al., 1999), establishing a link between this protein and the epilepsy. In 2009, the availability of heterozygous SV2A (+/-) mice as research tool enabled shedding light on the role of protein SV2A, revealing no motor differences but anxiety-like features in these mice compared with the WT (Lamberty et al., 2009), and a pro-epileptic phenotype (Crowder et al., 1999; Kaminski et al., 2008). Recently, a floxed SV2A mouse model has been produced with the Cre/loxP recombination system, this model allows invalidating the protein in CA3 hippocampal region, not followed by epileptic seizures (Menten-Dedoyart et al., 2016). Objectives: Perform a first behavioural phenotyping of SV2A lox/lox mice. Methodology: Two experiments were conducted in parallel to evaluate the effect of 3 different genotypes in the phenotype: WT (Grik4-/-, SV2A lox/lox), HZ (Grik4 +/-, SV2A lox/+) and cKO (Grik4 +/-, SV2A lox/lox) in male (n = 42) and female (n = 33) separately . Mice were housed individually along the experiment, with standard food and water ad libitum. After an acclimatization period of 2 weeks, anxiety-like features as well as exploration abilities were evaluated in an elevated plus-maze (EPM) single session of 5 minutes). 3 days later, spontaneous locomotor activity and habituation to the environment were measured during 1 hour, 3 consecutive days, in the activity chambers (ACT). Results: One-way ANOVA in EPM data presented no significant differences between groups, either in males or in females. A significant difference was found, between time spent in close arms vs open arms (p<0.01; η2p = 0.738 males; η2p = 0.805 females). Mixed between-within subjects ANOVA in ACT reflected no significant differences between groups in both sexes, regarding spontaneous locomotor activity and acclimatization to the activity chamber (p>0.05). Statistical significant differences were found between the 3 days (p<0.01; η2p = 0.716 males; η2p = 0.663 females). Conclusion: Results indicate that a decrease in the hippocampal expresion of SV2A protein does not lead to major behavioral changes. Regarding locomotor activity, the results found in heterozygous SV2A (+/-) mice are in line with (Lamberty et al., 2009), however, our mice did not present anxiety-like features, being necessary a global decrease in brain SV2A levels and not only a partial loss in a restricted region of the brain. Further analyses increasing the number of mice per group, will allow us to intensify our power value from 50-60% (females-males) up to 80%, with large effect size and a signification of p<0.05. An additional test to evaluate the spatial memory may help us better understand the effect a specific reduction in SV2A hippocampal expression has on the phenotype of mice. [less ▲]

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See detailEvolution of fast familiarity and novelty decisions in aging
Delhaye, Emma ULiege; Bastin, Christine ULiege; Moulin, Christopher et al

Poster (2017)

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See detailThe peculiar Of?p stars of the Magellanic Clouds
Nazé, Yaël ULiege; Szymanski, M.K.; Bagnulo, S. et al

Poster (2017)

The Of?p category gathers strongly magnetic massive stars which exhibit rotationally modulated spectral and photometric changes. Thanks to OGLE data, we investigated the photometric behaviour of five ... [more ▼]

The Of?p category gathers strongly magnetic massive stars which exhibit rotationally modulated spectral and photometric changes. Thanks to OGLE data, we investigated the photometric behaviour of five candidate Of?p stars in the Magellanic Clouds, revealing their variability. We combined these results to spectroscopic and spectropolarimetric investigations to further support the hypothesis that these are strongly magnetized O stars, analogous to the Of?p stars in the Galaxy. [less ▲]

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See detailIMPACT OF DYNAMIC SEATING ON CHILD’S BEHAVIOR AND CONCENTRATION IN CLASSROOM
Fettweis, Tatiana ULiege; Nattier, Elodie; Vanderthommen, Marc ULiege

Poster (2017)

Introduction Ergonomics and dynamism in classroom seem to influence schoolchildren’s behavior and concentration [1-2]. This study aimed to evaluate the influence of dynamic school furniture associated ... [more ▼]

Introduction Ergonomics and dynamism in classroom seem to influence schoolchildren’s behavior and concentration [1-2]. This study aimed to evaluate the influence of dynamic school furniture associated with a postural education on schoolchildren’s behavior and concentration in classroom. Methods Seventy-seven primary school children were divided into an experimental group (EG) (n=46, age=6.4, height=119.3cm, weight=23.2kg, body mass=16.5) and a control group (CG) (n=31, age=6.5, height=119.6cm, weight=23.1kg, body mass=16.1) and took part in this 2-school-year-follow-up cluster randomized controlled study including 4 phases of evaluation (E1 to E4) consisting of assessing the children’s behavior (sit or not / on task or not) from videos and their attention capacities with 2 different tests. In the first year, the EG was assessed before any intervention (E1), after having provided a triangular and dynamic cushion and 3 months of postural education (E2) and after 6 months using the cushion. In the second year, outcomes were assessed after 2 years using the cushion (E4). The CG had identical evaluation design but without any intervention. Results Compared with the CG, the children of the EG significantly improved their behavior at each step of the study. So, children’s spent proportionately more time sitting when ergonomic and dynamic seating was combined with postural education (p≤0.04) and they had more “on task” behavior (p≤0.001). Furthermore, the intervention tends to optimize the children’s attention (p<0.05). Conclusions The children’s behavior and concentration in the classroom was improved using ergonomic and dynamic furniture combined with a postural intervention. Further studies are needed to confirm those results. This study suggests that early prevention seems efficient to optimized schoolchildren’s behavior. [less ▲]

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See detailCyprinid Herpesvirus 3 ORF57 is an essential virulence factor
Boutier, Maxime ULiege; Gao, Yuan; Vancsok, Catherine et al

Poster (2017)

The genus Cyprinivirus comprises phylogenetically related viruses causing important economic losses in aquaculture. Its archetype species, the cyprinid herpesvirus 3 (CyHV 3) is the causative agent of ... [more ▼]

The genus Cyprinivirus comprises phylogenetically related viruses causing important economic losses in aquaculture. Its archetype species, the cyprinid herpesvirus 3 (CyHV 3) is the causative agent of mass mortalities in koi and common carp worldwide. Recently, we reported the development of a recombinant attenuated vaccine against CyHV 3 for mass vaccination of carp. This attenuated vaccine encodes a deletion encompassing ORF56 and ORF57. In the present study, we investigated the relative contribution of ORF56 and ORF57 in the safety observed for the ORF56-57 double deleted genotype. Single deleted recombinants were produced based on deletion-insertion of a galK cassette, inserting stop codons in frame with the sequence upstream of the deletion and disrupting the frame of the remaining sequence downstream of the deletion. Inoculation of these recombinants to carp demonstrated that the absence of ORF56 protein expression did not affect virulence; while ORF57 deletion led to an attenuation comparable to the one observed for the ORF56-57 deletion. To further demonstrate the role of ORF57 as a key virulence factor, a recombinant unable to express ORF57 protein was produced by insertion of multiple stop codons in the ORF. In vivo testing of this recombinant revealed a safety-efficacy profile comparable to the one observed for the vaccine candidate deleted for ORF56 and ORF57. Altogether, the present study demonstrates the role of ORF57 as a key virulence factor of CyHV 3 and identifies ORF57 orthologues encoded by other pathogenic cypriniviruses as gene candidates for production of attenuated recombinant vaccines. [less ▲]

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See detailWheel-running exercise during adolescence does not substantially affect cocaine conditioned place preference in male C57BL/6J mice
Lespine, Louis-Ferdinand ULiege; Tirelli, Ezio ULiege

Poster (2017)

Epidemiological studies suggest that physical exercise could have preventive properties against drugs of abuse vulnerability. Animal research showed that rats or mice housed with a running wheel (a model ... [more ▼]

Epidemiological studies suggest that physical exercise could have preventive properties against drugs of abuse vulnerability. Animal research showed that rats or mice housed with a running wheel (a model of aerobic exercise) can exhibit attenuated drug self-administration or drug-induced psychomotor hyperactivity in comparison with their sedentary counterparts. However, the few experiments using conditioned place preference (CPP) are conflicting (positive, negative or null effects of exercise). Aspects or deficiencies of the methods used in some studies, in particular the low sample size (median n=8), the absence of a baseline pre-conditioning session or a control group in the design or (when present) in the data analyses, make the whole picture of results difficult to understand, a situation which warrants further studies, possibly of a better quality than the previous ones. Objectives. Our purpose was to test whether wheel-running exercise during adolescence could impact the formation and long-term retention of CPP to cocaine in mice. Method. Male C57BL/6J mice were individually housed either with (n=32) or without (n=32) a running wheel from 35 days of age. Behavioral testing begun 3 weeks after such housing, all animals being first tested under saline for their baseline preference (white or black compartments). Then, mice underwent 10 once-daily conditioning sessions receiving peritoneal injections of 10 mg/kg cocaine and saline on alternate days (n=16). The white compartment (always non-preferred) was systematically associated to cocaine effects. Control mice received saline every day (n=16). One and 21 days after the last conditioning session, mice were tested for place preference under saline. CPP scores were analyzed with a priori single (cocaine vs saline) and crossed contrasts (testing the housing-by-drug interaction). Each contrast (t-test) incorporated the mean-square error (MSE) provided by a preliminary two-way fixed-model 2x2 ANOVA incorporating the housing condition (2 levels) and the drug treatment (2 levels) as between-group factors and time of testing as a blocking factor (8 levels). Estimates of effect sizes were provided by Cohen’s d calculated from ts and degree of freedom. Results. The two groups exhibited significant well-marked cocaine-induced CPP in both 1-day (d = 1.38 and d = 1.11 at ps < .001 one-tailed in exercised and sedentary mice) and 21-day post-conditioning tests (d = 1.09 and d = 1.15 at ps < .001 one-tailed in exercised and sedentary mice). The (small) effects underlying interaction between housing and the drug treatment were not significant for 1-day (d = 0.19 at p = .48 two-tailed; 95% CI -0.35 to 0.73) or 21-day post-conditioning tests (d = 0.05 at p = .87 two-tailed; 95% CI -0.49 to 0.59). Conclusion. If physical exercise in rodents “truly” impacts CPP induced by drugs of abuse under comparable experimental parameters - as suggested by some studies (either positively or negatively) -, our results indicate that the size of such effects may be quite small, an information rarely reported in the literature. [less ▲]

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See detailLes pentes d’évolution des CTX, de la phosphatase alkaline osseuse et du P1NP sont associées à celles de la PTH en hémodialyse chronique.
Maillard, Nicolas; Warling, Xavier; Moonen, Martial et al

Poster (2017)

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See detailIsotopic compositions of North Sea marine mammals
Damseaux, France ULiege; Pomeroy, Paddy; Sierbert, Ursula et al

Poster (2017)

For over a decade, the North Sea has been undergoing significant changes due to global changes and overfishing. We conducted meta-analyses on marine mammals sampled in the North Sea to test the potential ... [more ▼]

For over a decade, the North Sea has been undergoing significant changes due to global changes and overfishing. We conducted meta-analyses on marine mammals sampled in the North Sea to test the potential impact of changes in fish stocks on feeding behaviour of grey seals, harbour seals and harbour porpoises. Data included δ13C and δ15N values measured in blood cells and muscles from the three species. SIBER, an isotopic niche quantification approach, is used to highlight potential dietary similarity and thus competition between marine mammal species. Harbour seals sampled in Germany showed the highest δ15N values, reflecting a trophic position at the top of the food web, alongside grey seals. In contrast, harbour porpoises sampled from Germany displayed the lowest trophic position. The ellipse overlapping between German harbour and grey seals was very important, showing similarity in, and therefore potential competition for, food sources. On the other hand, the harbour seal and the harbour porpoise of Germany displayed extended ellipse size compared to the grey seal. This may be due to a more diverse diet and, perhaps, a more opportunistic foraging behaviour than grey seals. Surprisingly, another group of grey seals sampled at Isle of May, Scotland displayed lower δ15N values and a very small ellipse size compared to grey seals from Germany, presumably being even more selective in their prey choice. Nevertheless, comparing the trophic position of the groups of grey seals requires caution as the isotopic baseline differed between the two sampling areas. This study allowed the determination of the competition, the spatial variations and the trophic niches of marine mammals in the North Sea and will, at the end, evaluate the effects of the changes in the North Sea on the ecology of marine mammals. [less ▲]

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See detailCharacterization of metallic trace elements in soils by portable X-ray fluorescence spectrometry
Rosière, Clément; Godin, Bruno; Liénard, Amandine ULiege et al

Poster (2017)

1. Introduction X-ray Fluorescence Spectrometry (XRF) is undeniably a valuable asset for the simultaneous determination of mineral elements. This is a fast, non-destructive and inexpensive method in ... [more ▼]

1. Introduction X-ray Fluorescence Spectrometry (XRF) is undeniably a valuable asset for the simultaneous determination of mineral elements. This is a fast, non-destructive and inexpensive method in comparison with conventional analysis methods. The recent development of portable spectrometers (pXRF) further increases the potential of the XRF technique in environmental purposes by bringing the device to the field. This work focused on trace elements determination (Cu, Zn, Pb, Ni, Cr and As), most of which are subject to specific regulations, especially for sewage sludge (expect As) and contaminated soil management. In Wallonia, the reference method is based on aqua regia (HCl+HNO3, ISO 11466) digestion followed by Atomic Absorption Spectrometry (AAS) or Inductively-coupled plasma atomic emission- or mass- spectrometry (ICP-AES/ICP-MS). It is established that aqua regia digestion-based analysis underestimates the total content of elements because it does not completely digest silicates, while XRF is supposed to measure total content. To assess the performance of a pXRF (S1 Titan 600, Bruker), we compared the prediction values with the values from the aqua regia digestion for some reference values in soils. 2. Material and methods Seventeen soils (mainly agricultural soil), all of which were already evaluated for concentration of some metallic trace elements, were analyzed by pXRF in desktop configuration with XRF cells (Ø 40 mm, Prolene film 4µm) according to a validation process and were compared to their current aqua regia digestion-AAS values. Soil selection was based on results of a principal component analysis (PCA) using metallic trace elements and major elements (Ca, Mg, K, P, Fe) aqua regia contents, followed by a hierarchical cluster analysis (Ward’s method) to extract samples as diversified as possible. In addition, three inter-laboratory reference materials from BIPEA were subjected to the same protocol to carry out checks on the laboratory experiments. All samples were air-dried, sieved and crushed to 200 µm. Time measurement was set to 30 seconds in dual phase (60 seconds total). In order to assess the validity of the pXRF, the accuracy profile’s method [1] was chosen. Under intermediate precision conditions (5 days and 3 repetitions/day), results were calculated as the mean of 3 successive readings. The accuracy profile allows determining an interval which will contain 95% of the measurements. This interval was then compared to an acceptability interval, which was fixed at ± 20% of the reference value, to vouch for the validity. Reference values of each metallic trace element were calculated as the mean of 5 series of measurements according to an aqua regia digestion-AAS method. For the purpose of improving the trueness, two types of regression were applied between XRF and reference values: a simple linear regression and a FREML regression [2]. The advantage of the latter is that it can take into account errors on both X and Y variables. Dataset was split into one calibration set (2/3) and one validation set (1/3). In addition, the performance of the pXRF was compared to a laboratory wavelength-dispersive X-ray fluorescence spectrometer (WDXRF) supposed to give more reliable results and total contents. 3. Results and discussion Strong linear correlations were found in soils for Cu, Zn or Pb (R² > 0.99) between pXRF and aqua regia digestion-AAS. This linear correlation was very poor for Cr, probably due to internal calibration issues. Figure 1 shows the Zn accuracy profile, where the underestimation by pXRF can be seen. A simple slope and y-intercept correction of pXRF data could generally restore the trueness (bias) to improve the accuracy on a larger concentration range. However, concentration levels close to detection limits have a higher degree of random variability. This can be explained by the Horwitz curve where random variability increases with lower concentrations. This emphasizes the need of multiplying the number of measurements/readings. The comparison made of the pXRF with the WDXRF showed that the pXRF underestimates the metallic trace elements content. Indeed, the pXRF results were lower than WDXRF results. But, in terms of prediction of the reference values, the pXRF seems to be only slightly worse than the WDXRF. This shows the power of the portable XRF to predict AAS reference values at a low cost. Figure 1. Zinc Accuracy profile. Red short dotted line: Acceptance limits. Orange long dotted line: Tolerance limits 4. Conclusions S1 Titan XRF is an interesting tool and easy to use for the prediction of metallic trace elements content in soils. However, to predict reference values (aqua regia digestion-AAS) with sufficient accuracy, direct measurements are not suitable and a specific XRF calibration is recommended. A simple linear or FREML regression is adequate to improve the accuracy of the measured values in some cases, depending on the wanted future application. 5. References [1] M Feinberg, M Laurentie 2010. Validation des méthodes d’analyse quantitative par le profil d’exactitude. Cah. Tech. l’INRA No Special, 139, 2010. [2] Analytical Methods Committee (AMC). Fitting a linear functional relationship to data with error on both variables [technical brief no. 10]. R. Soc. Chem. 1(10), 2002. [less ▲]

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See detailThe role of large trees in the biomass production of heterogeous forest
Ligot, Gauthier ULiege; Gourlet-Fleury; Ouedraogo, Dakis-Yaoba ULiege et al

Poster (2017)

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See detailLife cycle assessment (LCA) of an indoor pilot aquaponics production facility in Belgium
Gennotte, Vincent ULiege; Forchino, Andrea Alberto; Mélard, Charles ULiege et al

Poster (2017)

Aquaponics arouses a growing interest as a sustainable way to produce fish and vegetables in an integrated system. The main advantages of aquaponics production are water saving and reduced sewage that can ... [more ▼]

Aquaponics arouses a growing interest as a sustainable way to produce fish and vegetables in an integrated system. The main advantages of aquaponics production are water saving and reduced sewage that can be achieved through nutrient cycling. On the other side, several challenges raised from this method as it is energy demanding and often provides sub-optimal growing conditions for fish and/or vegetables in coupled systems (Junge et al., 2017). Sustainability of such systems must be assessed through a global approach taking into account not only the production processes but encompassing also upstream (structure and equipment) and downstream (product distribution and by-product valorisation) impacts. Life Cycle Assessment (LCA) is a method that can be used to investigate the sustainability of production systems such as aquaponics. We performed a full analysis of an aquaponics pilot system built in Belgium in 2017. The LCA covers the infrastructure construction, presently completed, and a prediction of system running based on a production model. [less ▲]

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See detailModified swim test as a model of enhanced contextual conditioning during depression: expression of GSK3 beta and effects of antidepressant treatment
Markova, N; Shevtsova, S; Vignisse, Julie et al

Poster (2017)

Impaired brain plasticity is a well-established pathophysiologic feature of depression, but little is known about an enhancement of depression-associated cognitive processing. We studied a novel paradigm ... [more ▼]

Impaired brain plasticity is a well-established pathophysiologic feature of depression, but little is known about an enhancement of depression-associated cognitive processing. We studied a novel paradigm that potentially models the augmented acquisition of adverse memories during the development of a depressive-like state in mice. We used a modification of the classic two-day protocol of a mouse Porsolt’s test with an additional session on Day 5 following the initial swim session. On the last day of testing, floating behavior, a parameter of helplessness, was increased in naïve mice that was accompanied by a reduction in the pGSK3b/GSK3b ratio and increased levels of brain GSK3b mRNA. The increase in GSK3b mRNA in prefrontal cortex during delayed testing session correlated with increases in floating behavior, which is not observed in the classic Porsolt’s paradigm. Replacement of the last swim session with exposure to the context of testing resulted in increased GSK3b mRNA level similar to the effect of swimming, while exclusion of the last swim session prevented these changes. The behavioral changes and the alterations in GSK3b gene and protein levels were prevented by 2-week treatment with a low dose of classical antidepressant tricyclic imipramine (7.5 mg/kg/day), or vitamin B1 (thiamine) (200 mg/kg/day), or with the highly bioavailable thiamine precursor benfotiamine (200 mg/kg/day). Our study also demonstrated, for the first time, the antidepressant-like properties of vitamin B1 and its pre-cursor, in a pre-clinical model of depression. Thus, the new forced swim test paradigm models the enhanced contextual conditioning of adverse memories so the individual animals with distinct susceptibility to this syndrome to be differentiated. [less ▲]

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See detailBenfotiamine Treatment Activates Nrf2/ARE Pathway and is Neuroprotective in a Transgenic Mouse Model of Tauopathy
Tapias, Victor; Jainuddin, Shari; Ahuja, Manuj et al

Poster (2017)

Impaired glucose metabolism, decreased levels of thiamine (vitamin B1) and its phosphate esters, and downregulated activity of thiamine-dependent enzymes, such as pyruvate dehydrogenase, alpha ... [more ▼]

Impaired glucose metabolism, decreased levels of thiamine (vitamin B1) and its phosphate esters, and downregulated activity of thiamine-dependent enzymes, such as pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, and transketolase have been linked to Alzheimer’s disease (AD). Thiamine-deficient mice exhibit increased amyloid deposition, tau hyperphosphorylation, and oxidative damage1. Experimental evidence has shown that benfotiamine (BFT), a synthetic S-acyl derivative of thiamine, rescued cognitive deficits and reduced amyloid burden in APP/PS1 mice2. We investigated whether BFT confers neuroprotection against tau phosphorylation and the generation of neurofibrillary tangles (NFTs) ‒ which causes frontotemporal dementia in humans ‒ in a mouse model of tauopathy. Exposure to BFT resulted in increased lifespan, behavioral improvement, reduced and glycated tau and NFTs, and prevented neuronal death in P301S transgenic (TG) mice. In addition, BFT administration significantly ameliorated mitochondrial dysfunction, attenuated oxidative damage, and decreased the expression of several pro-inflammatory mediators, consistent with a possible activation of the Nrf2/ARE neuroprotective pathway. Accordingly, we found that BFT (but not thiamine) triggers the expression of Nrf2/ARE-dependent genes in wild-type (WT) but not in Nrf2-deficient fibroblasts. Our findings suggest that BFT is a promising therapeutic agent for the treatment of tauopathies. [less ▲]

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See detailContribution of mesoscale eddies to Black Sea ventilation
Capet, Arthur ULiege; Mason, Evan; Pascual, Ananda et al

Poster (2017)

The shoaling of the Black Sea oxycline is one of the most urgent environmental issues in the Black Sea. The permanent oxycline derives directly from the Black Sea permanent stratification and has shoaled ... [more ▼]

The shoaling of the Black Sea oxycline is one of the most urgent environmental issues in the Black Sea. The permanent oxycline derives directly from the Black Sea permanent stratification and has shoaled alarmingly in the last decades, due to a shifting balance between oxygen consumption and ventilation processes (Capet et al. 2016). The understanding of this balance is thus of the utmost importance and requires to quantify 1) the export of nutrients and organic materials from the shelf regions to the open sea and 2) the ventilation processes. These two rocesses being influenced by mesoscale features, it is critical to understand the role of the semi-permanent mesoscale structures in horizontal (center/periphery) and vertical (diapycnal and isopycnal) exchanges. A useful insight can be obtained by merging observations from satellite altimeter and in situ profilers (ARGO). In such composite analyses, eddies are first automatically identified and tracked from altimeter data (Mason et al. 2014, py-eddy-tracker). Vertical ARGO profiles are then expressed in terms of their position relative to eddy centers and radii. Derived statistics indicate how consistently mesoscale eddies alter the vertical structure, and provide a deeper understanding of the associated horizontal and vertical fluxes. However, this data-based approach is limited in the Black Sea due to the lower quality of gridded altimetric products in the vicinity of the coast, where semi-permanent mesoscale structures prevail. To complement the difficult analysis of this sparse dataset, a compositing methodology. is also applied to model outputs from the 5km GHER-BHAMBI Black Sea implementation (CMEMS BS-MFC). Characteristic biogeochemical anomalies associated with eddies in the model are analyzed per se, and compared to the observation-based analysis. Capet, A., Stanev, E. V., Beckers, J.-M., Murray, J. W., and Grégoire, M.: Decline of the Black Sea oxygen inventory, Biogeosciences, 13, 1287-1297, doi:10.5194/bg-13-1287-2016, 2016. Mason, Evan, Ananda Pascual, and James C. McWilliams. "A new sea surface height–based code for oceanic mesoscale eddy tracking." Journal of Atmospheric and Oceanic Technology 31.5 (2014): 1181-1188. [less ▲]

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See detailMetabolomic study and pharmacological valorisation of species of the genus Psiadia endemic to Reunion and Mauritius islands.
Mahadeo, Keshika; Grondin, Isabelle; Kodja, Hippolyte et al

Poster (2017)

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See detailHow does the connectivity index change through year in an agricultural catchment?
Cantreul, Vincent ULiege; Degré, Aurore ULiege

Poster (2017)

The emerging concept of hydrological connectivity is difficult to quantify. Some indices have been proposed. The most cited is Borselli’s one. It gives the advantage to visualize connectivity at watershed ... [more ▼]

The emerging concept of hydrological connectivity is difficult to quantify. Some indices have been proposed. The most cited is Borselli’s one. It gives the advantage to visualize connectivity at watershed scale with very few inputs. But it is not a dynamic index and the resulting map is not time dependent. However, vegetation cover changes through year and possibly affects the connectivity dynamics. The objective of this poster is to show the evolution of the CI during the year looking at a few “strategic” times. Moreover, the study permits to identify a few “key locations” in the watershed, for example permanent disconnections or at the opposite constantly connected fields. The CI was calculated in a 124ha catchment (Hevillers), in the loess belt, in Belgium. Land use is agricultural with mostly cereals, sugar beets and potatoes, little area with wood, road, path or grass strip. Used weighting factor is soil loss ratio. It is between 0 and 1 and translates the protection offered to the soil by the crop. In winter (January), cereals have the most connected fields because of almost bare soils. Cover crops on sugar beets and potatoes fields decrease connectivity, except for one big field not far from the outlet. But rainfalls are generally not so erosive during this period. In spring (March and May), the cereals have a decreasing CI with plants growth covering the soil. On the opposite, sugar beets and potatoes are planted and bare soils in spring involve much higher connectivity index. The effect of grass strip is strong for sugar beet field situated uphill and underlines the importance of such mitigation measures. In summer (July), the whole watershed is much more disconnected and it does not represent the most risky part of the year in terms of erosion. The end of the year is related to harvesting and consequent bare soil in September for potatoes and November for the rest. In conclusion, the IC is an easy tool to estimate connectivity in a watershed. With the evolution during the year using soil loss ratio in the calculation, it permits to visualize dynamically the connectivity pattern and to localize erosive parts of the catchment for the crop rotation. With a global view on several years, it could be helpful to erosion managers to think about best long-term location of mitigation measures in the watershed. [less ▲]

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See detailMolecular characterization of five metalloproteases (Mep1-5) in Microsporum audouinii strains circulating in Belgium.
Aouini, Imen ULiege; SACHELI, Rosalie ULiege; DARFOUF, Rajae ULiege et al

Poster (2017)

Background Microsporum audouinii (M. audouinii) is an anthropophilic dermatophyte responsible for tinea capitis in young children. Infections caused by this species are very contagious and are responsible ... [more ▼]

Background Microsporum audouinii (M. audouinii) is an anthropophilic dermatophyte responsible for tinea capitis in young children. Infections caused by this species are very contagious and are responsible for outbreaks in schools and communities. Different proteases are produced by dermatophytes to digest tissue keratin. Among these proteases metalloproteases (Mep) have already been described as potential virulence factors in different zoonotic species such as Trichophyton mentagrophytes and Microsporum canis. In the present study, primers targeting five metalloproteases Mep1-5 have been designed to screen a large scale of Microsporum audouinii strains isolated in Belgium. Material and methods A total of 84 strains of M.audouinii were included in this study. This concerned 2 reference strains (IHEM 2758 and 2214) and 82 strains isolated from patients refered to the Mycology Laboratory of the University Hospital of Liègeduring the 2013-2016 period. The strains were identified as M. audouinii by microscopy and confirmed by ITS sequencing. Primers were newly designed based on nucleotide sequences of the genes MEP1-5 available in GenBank database for the close related species M. canis and using primer Blast (NCBI-NIH) Results Among the analyzed strains, the presence of at least one gene encoding for Mep1-5 was revealed in 93% (78/84) of the M. audouinii strains with 80 % (67/84) being positive for the five Meps (1-5). The percentage of detection was 89% (75/84) for Mep1, 88% (74/84) for Mep2 and Mep 5 and 85% (71/84) for Mep3. Finally, Mep4 was successfully amplified in 92% (77/84) of the isolates. In total, 7% (6/84) of the strains did not express any Mep gene. An internal control of amplification (ITS sequence) was also included to exclude a false negative result due to amplification inhibitors. Conclusion The presence of the Mep1-5 genes in M. audouinii strains circulating in Belgium was confirmed in this study. Fairly close percentages of expression of these genes let us think that all tested Meps could be implicated in the virulence process of M. audouinii strains. Next step will be the in vitro biochemical characterization of the expression of these Meps in M. audouinii. Another class of endoproteases called subtilisins has been recently considered to be involved in virulence process. Their presence will also be further checked in M. audouinii strains. [less ▲]

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See detailThiamine and lipophilic thiamine precursors protect against oxidative damage in cultured neuroblastoma cells
Sambon, Margaux ULiege; Wins, Pierre; Bettendorff, Lucien ULiege

Poster (2017)

Recent evidence suggests that thiamine (vitamin B1) and some of its derivatives can exert prominent neuroprotective effects in the mammalian brain, particularly in mouse models of Alzheimer’s disease and ... [more ▼]

Recent evidence suggests that thiamine (vitamin B1) and some of its derivatives can exert prominent neuroprotective effects in the mammalian brain, particularly in mouse models of Alzheimer’s disease and tauopathies. As orally administered thiamine crosses intestinal and blood-brain barriers only slowly, precursors with higher bioavailability e.g. sulbutiamine, benfotiamine and dibenzoylthiamine, have been developed. We investigated the protective effects of thiamine and those precursors in neuroblastoma cells cultured in a medium containing minimal amounts of thiamine (10 nM). We induced oxidative stress by incubating the cells (24h) in the presence of the neurotoxic agent paraquat (0.25 mM). This treatment reduced cell viability by 40%. When thiamine or the precursors were present simultaneously, we observed protective effects by the precursors while free thiamine was ineffective.Dibenzoylthiamine was most efficient, affording complete protection of cells at 10-20 μM. It also caused the highest increase in intracellular thiamine, suggesting that the protection from oxidative damage is linked to increased levels of free thiamine (rather than thiamine diphosphate) in the neuroblastoma cells. These results and others from our laboratory raise the possibility that dibenzoylthiamine might useful as a neuroprotective agent in neurodegenerative disease. [less ▲]

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See detailDibenzoylthiamine, a lipophilic thiamine precursor, protects against oxidative damage in neuroblastoma cells
Sambon, Margaux ULiege; Wins, Pierre; Bettendorff, Lucien ULiege

Poster (2017)

Recent evidence suggests that thiamine (vitamin B1) and some of its derivatives can exert prominent neuroprotective effects in the mammalian brain, particularly in mouse models of Alzheimer’s disease and ... [more ▼]

Recent evidence suggests that thiamine (vitamin B1) and some of its derivatives can exert prominent neuroprotective effects in the mammalian brain, particularly in mouse models of Alzheimer’s disease and tauopathies. As orally administered thiamine crosses intestinal and blood-brain barriers only slowly, precursors with higher bioavailability e.g. sulbutiamine, benfotiamine and dibenzoylthiamine, have been developed. We investigated the protective effects of thiamine and those precursors in neuroblastoma cells cultured in a medium containing minimal amounts of thiamine (10 nM), but sufficient to sustain normal growth. We induced oxidative stress by incubating the cells (24 h) in the presence of the neurotoxic agent paraquat (0.25 mM). This treatment reduced cell viability by 40%. When thiamine or the precursors were present simultaneously, we observed protective effects by the precursors while free thiamine was ineffective. Dibenzoylthiamine was most efficient, affording complete protection of cells at 10-20 µM. It also caused the highest increase in intracellular thiamine, suggesting that the protection from oxidative damage is linked to increased levels of free thiamine (rather than thiamine disphophate) in the neuroblastoma cells. The mechanism of this protective effect is presently under investigation. These results and others from our laboratory raise the possibility that dibenzoylthiamine might useful as a neuroprotective agent in neurodegenerative disease. [less ▲]

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See detailNitrous oxide dynamic in sea ice
Kotovitch, Marie ULiege; Fripiat, François ULiege; Deman, Florian et al

Poster (2017)

Fluctuations in greenhouse gases (GHGs) concentration alter the energetic budget of the climate system. There is high confidence that natural systems related to snow, ice and frozen ground are affected ... [more ▼]

Fluctuations in greenhouse gases (GHGs) concentration alter the energetic budget of the climate system. There is high confidence that natural systems related to snow, ice and frozen ground are affected. Nitrous oxide (N2O) is one of the potent GHG naturally present in the atmosphere, but witch has seen his concentration growing since industrial era. N2O has a lifetime in the atmosphere of 114 years and a global warming potential 300 time higher than that of CO2. Yet, there are still large uncertainties and gaps in the understanding of the cycle of this compound through the ocean and particularly in sea ice. Sources and sinks of N2O are therefore still poorly quantified. The main processes (except the transport processes) involved in the N2O cycle within the aquatic environment are nitrification and denitrification. To date, only one study by Randall et al. 2012 present N2O measurements in sea ice. Randall et al. pointed out that sea ice formation and melt has the potential to generate sea-air or air-sea fluxes of N2O, respectively. Study on ammonium oxidation and anaerobic bacterial cultures shows that N2O production can potentially occur in sea ice. Denitrification can act as a sink or a source of N2O. In strictly anaerobic conditions, N2O is removed by denitrification. However, denitrification can also occur in presence of O2 at trace level concentrations (<0.2 mg L-1), and in these conditions there is a large N2O production. Recent observations of significant nitrification in Antarctic sea ice shed a new light on nitrogen cycle within sea ice. It has been suggested that nitrification supplies up to 70% of nitrate assimilated within Antarctic spring sea ice. Corollary, production of N2O, a by-product of nitrification, can potentially be significant. This was recently confirmed in Antarctic land fast ice in McMurdo Sound, where N2O release to the atmosphere was estimated to 4 µmol.m-2.yr-1. This assessment is probably an underestimate since it only accounts for dissolved N2O while a significant amount of N2O is likely to occur in the gaseous form like N2, O2 and Ar. This poster address the issue related to the production of N2O within sympagic microorganisms. What process is dominant and how much N2O is produced? The determination of the isotopic composition of N2O using cavity enhanced laser absorption spectroscopy technique (Off-axis ICOS) will allow us to determine the origin of these processes. It will be based on the relative isotope abundance values and site preference data in previous studies. [less ▲]

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See detailCellular and genomic disease signature of peripheral blood mononuclear cells in patients with malignant pleural mesothelioma
Mallon, Zachary R.; Poulet, Christophe ULiege; Enstrom, Amanda et al

Poster (2017)

Background: Recent data on the incidence malignant pleural mesothelioma (MPM) and the continued large-scale use of asbestos throughout the developing world portends an epidemic of asbestos-related disease ... [more ▼]

Background: Recent data on the incidence malignant pleural mesothelioma (MPM) and the continued large-scale use of asbestos throughout the developing world portends an epidemic of asbestos-related disease. MPM is an aggressive and fatal cancer with few treatment options. Recent advances in large scale genomic and high throughput cellular analyses now provide the tools to more easily attain markers of disease status and potential responsiveness to immunotherapeutics. Materials and Methods: Here we present pre-treatment cellular and genomic biomarker data on a cohort of chemotherapy-naïve MPM patients, and demographically matched healthy donors (HD). MPM patients were enrolled in a Phase 1b study utilizing CRS-207, a live, attenuated Listeria monocytogenes strain engineered to express the tumor-associated antigen, mesothelin. Four different multi-color flow cytometry panels were used to provide resolution on major immune cell populations of T cells, γδ T cells, B Cells, dendritic cells, monocytes, and natural killer cells. Together, these panels provided deeper resolution on 39 distinct subpopulations of major immune cell subsets. RNA from these cells was used to perform multiplex gene expression analysis on 770 genes using the Nanostring nCounter PanCancer Pathway Panel. Results: FACS analysis yielded numerous subpopulations with statistically significant differences between MPM patients and healthy controls. Differences in immune populations were analyzed by median and significant findings included populations of CD4+ T cells, CD8+ T cells, B cells, classical monocytes, and monocytic myeloid derived suppressor cells*. Class comparison and hierarchical clustering of gene expression data revealed genomic markers that were significantly expressed in MPM compared to healthy controls. Immune subset deconvolution of gene expression data provided similar findings as FACS analysis and corroborated this disease signature across experimental platforms. Conclusions: Understanding a patient’s biological disease signature can aide in diagnosis, as well as in making informed choices about therapies amidst the complex and broadening immunotherapeutic landscape. Until recently, existing biomarker data in MPM has been limited to a small number of serological markers and limited immune analysis. Here, we present the first comprehensive report of a MPM disease signature from the cellular and genomic perspectives. Correlation of patient baseline disease signatures with treatment outcome may yield biomarkers predictive of treatment efficacy. Predictive signatures are being investigated in the on-going Phase 1b study of CRS-207 and chemotherapy, as well as in the Phase 2 study of CRS-207 with pembrolizumab in MPM patients who failed prior treatment. *Inclusion of additional subjects confirmed the significance of all immune cell subsets except for MDSCs. [less ▲]

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See detailRADIOGRAPHIC VERTEBRAL ABNORMALITIES IN WILD EUROPEAN EELS (Anguilla anguilla)
Busoni, Valeria ULiege; Sonny, Damien; Antys, Martine

Poster (2017)

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See detailThresholds of plankton community change in a Mediterranean coastal area : results from a long-term (1979-2014) time series
Goffart, Anne ULiege; Collignon, Amandine; Lejeune, Pierre et al

Poster (2017)

In the Mediterranean Sea, which has been identified as a hotspot for climate change, there is evidence for impacts of climate change on marine organisms. However, the lack of information on environmental ... [more ▼]

In the Mediterranean Sea, which has been identified as a hotspot for climate change, there is evidence for impacts of climate change on marine organisms. However, the lack of information on environmental drivers and associated thresholds limits our ability to forecast possible changes in biodiversity and ecological interactions. In this presentation, we use a unique long-term (1979-2014) time series performed in a Mediterranean coastal area unbiased by local anthropogenic pressure (Bay of Calvi, Corsica) to understand how climate variation controls phyto- and zooplankton dynamics and possibly affect artisanal and small-scale fisheries exploiting areas near the coast. From high-frequency field data, we describe a mechanism that links winter physics, nutrient replenishment of the surface layer and plankton dynamics under the different combinations of meteorological conditions that occurred during the 36 years of observations. We identify threshold values of physical variables below and above which they strongly impact nutrient availability, phyto- and zooplankton bloom characteristics and seasonality succession of plankton functional groups. We provide identification of environmental thresholds beyond which diatoms, which are crucial for sustaining fish populations, collapse. We discuss links between climate-related changes in plankton availability, fish recruitment and the success of small-scale fisheries. We show that the mechanism we identified from the 1979 to 2014 period of observation in the Bay of Calvi is pertinent to other Northwestern Mediterranean areas, stressing the importance of winter conditions in determining the state of Mediterranean pelagic ecosystems. We highlight that the thresholds obtained from our long-term time series provide key information for improving model scenarios of the impact of climate change on Mediterranean ecosystems. [less ▲]

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See detailGlycaemic Control in ICU: Stable Patients Tend to Remain Stable
Uyttendaele, Vincent ULiege; Dickson, Jennifer L.; Stewart, Kent et al

Poster (2017)

Objective: STAR is a glycaemic control (GC) protocol with proven safety and performance. It uses a cohort-based 2D stochastic model of model-based, patient-specific insulin sensitivity (SI). Given current ... [more ▼]

Objective: STAR is a glycaemic control (GC) protocol with proven safety and performance. It uses a cohort-based 2D stochastic model of model-based, patient-specific insulin sensitivity (SI). Given current SI, it predicts a range of future SI values to dose insulin based on specified risk of hypoglycaemia. This study examines whether considering the prior change in SI (%SI) as an input to a 3D stochastic model can reduce the conservatism and provide more accurate estimates. Method: Metabolic data from 3 clinical ICU cohorts (819 episodes and 68629 hours) in Christchurch (SPRINT and STAR) and Hungary (STAR) are used. Triplets (%ΔSIn, SIn, SIn+1) are created for every hour to create a 3D stochastic model with inputs (%ΔSIn, SIn) and output SIn+1. The 5-95th percentile prediction width of the 3D model is compared at every %ΔSIn value to the 2D model 5-95th width. A narrower band for the 3D model indicates the 2D model used is over-conservative and GC could be more aggressive, while a wider bound indicates increased risks. Results: The 2D model is over-conservative for 77% of hours, mainly where %ΔSI is within an absolute 25% change, with 25-40% narrower prediction ranges. Predictive power is similar for both models, but much closer to the ideal value of 90% for the 3D model, indicating greater patient-specificity. Cross-validations show these results generalise well to different ICU populations. Conclusions: By reducing prediction range for 77% of hours across 3 ICU cohorts, predominantly where SI is stable, the new 3D model shows stable patients tend to remain stable in terms of %ΔSI. The new model better characterises patient-specific response to insulin, allowing more optimal dosing while increasing performance and safety. [less ▲]

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See detailEchinococcose alvéolaire: le point en Wallonie
Cambier, Audrey ULiege

Poster (2017)

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See detailRaman imaging for diagnostic application
Malherbe, Cédric ULiege; Gilbert, Bernard ULiege; Eppe, Gauthier ULiege

Poster (2016, December 19)

Specific dynamic biochemical compositions and molecular structures characterise healthy cells and tissues. This also stands for pathologies or cellular abnormalities which are accompanied by biochemical ... [more ▼]

Specific dynamic biochemical compositions and molecular structures characterise healthy cells and tissues. This also stands for pathologies or cellular abnormalities which are accompanied by biochemical and molecular changes. Raman spectroscopy can provide unique inputs to correlate the molecular composition and its variations with the diagnosis. In addition, the presence of metal nanoparticles in the molecule vicinity induces a remarkable enhancement (up to 1000000 times) of the Raman signal of small molecules (known as SERS effect). It opens towards applications in the field of trace analysis. In preparation for clinical applications, we evaluate, by Raman mapping, the signal detected from a SERS target deposited on solid state support. [less ▲]

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See detailIntegrated microfluidic device for the synthesis of SERS substrates
Emonds-Alt, Gauthier ULiege; Malherbe, Cédric ULiege; Monbaliu, Jean-Christophe ULiege et al

Poster (2016, December 19)

The goal of this work is to develop a quantitative analytical method for the glyphosate and its major metabolite the aminomethylphosphonic acid (AMPA) by coupling microfluidics with surface enhanced Raman ... [more ▼]

The goal of this work is to develop a quantitative analytical method for the glyphosate and its major metabolite the aminomethylphosphonic acid (AMPA) by coupling microfluidics with surface enhanced Raman spectroscopy (SERS). This work is divided into 4 axes : (a) bimetallic nanoparticles synthesis in a microreactor, (b) nanoparticles functionnalisation, (c) SERS quantification of glyphosate/AMPA and (d) miniature prototype development composed of axes a to c. The first section aims at the synthesis of bimetallic nanoparticles (Au-Ag, Au-Cu and Ag-Cu) in a microfluidic device. This microfluidic device integrates a first section for the synthesis of monometallic nanoparticles (core) and a second section for the reductive deposition of a second metal (shell). The second axe of this work deals with the functionnalisation of nanoparticles to improve the selectivity of this method for the selected analytes. Two types of functionnalisation are studied : PEGylation (a) with multi-arm PEG ending with -SH and/or (b) with linear heterobifunctionnal PEG. The third axe consists in measuring quantitatively glyphosate and AMPA in different matrices, such as water, urine and fruit juice. Finally, the forth axe deals with the development of a portable detection system to perform on-site analysis. This prototype integrates the microfluidic device for nanoparticles synthesis, their functionnalisation, an injection port followed by a mixing section for the sample and an integrated Raman spectrometer for in-line analysis. [less ▲]

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See detailPrésentation du projet Economie et pratiques des sociétés gravettiennes dans le Nord-Ouest de l’Europe (ECOPRAT)
Touzé, Olivier ULiege; Salomon, Hélène ULiege; Goutas, Nejma et al

Poster (2016, December 17)

Nous présentons dans cette contribution un projet collectif porté par le Service de Préhistoire de l’Université de Liège et l’équipe Ethnologie préhistorique de l'unité mixte de recherche du CNRS ... [more ▼]

Nous présentons dans cette contribution un projet collectif porté par le Service de Préhistoire de l’Université de Liège et l’équipe Ethnologie préhistorique de l'unité mixte de recherche du CNRS Archéologie et Sciences de l'Antiquité. Le projet « ECOPRAT » entend contribuer au renouvellement des connaissances relatives aux sociétés gravettiennes du Nord-Ouest européen, et prolonger ainsi la dynamique de recherche actuelle touchant ces sociétés (Bodu et al., 2013). Il s’inscrit dans une perspective pluridisciplinaire envisagée grâce à l’association d’études technologiques, tracéologiques et archéozoologiques. Quatre sites majeurs du Nord-Ouest européen ont été retenus : les gisements de plein air de Maisières-Canal (Prov. Hainaut, Belgique) et d’Ormesson – Les Bossats (Seine-et-Marne, France), et les grottes de Goyet (Prov. Namur, Belgique) et du massif d’Arcy-sur-Cure (Yonne, France). Les sites sélectionnés possédant des historiques de recherche spécifiques, la stratégie d’analyse adoptée pour chacun d’eux est développée en adéquation avec l’état des connaissances actuelles. Les recherches sont ainsi guidées par l’obtention de données inédites qui doivent permettre, à terme : 1) une lecture renouvelée et croisée des industries lithiques et osseuses, ainsi que des ensembles fauniques et des matières colorantes découverts dans ces gisements, 2) un rééquilibrage quantitatif et qualitatif de l’intégration des différentes sources documentaires disponibles, la recherche s’étant davantage concentrée depuis plus d’un siècle sur les données de l’industrie lithique. Les deux gisements de plein air réunissent les conditions indispensables au développement d’une approche interdisciplinaire permettant de croiser objets et méthodes d’étude, afin de proposer une lecture fine des comportements techniques et économiques des sociétés gravettiennes. A cet égard, cette contribution est l’occasion de présenter les premiers résultats obtenus sur le site de Maisières-Canal. Les recherches réalisées jusqu’à présent permettent de réévaluer l’importance de l’industrie sur matières dures d’origine animale, au sein de laquelle le travail de l’ivoire de mammouth se révèle particulièrement significatif. En outre, le corpus faunique traduit une exploitation récurrente du lièvre, ce type de comportement s’avérant singulier au regard de ce qui est habituellement documenté chez les sociétés du Gravettien. Parmi les différentes activités susceptibles de laisser une trace archéologique, l’usage de matières colorantes apparaît particulièrement discret dans le cadre spatio-temporel considéré. Cet usage est cependant avéré dans les grottes d’Arcy-sur-Cure (grottes du Renne et du Trilobite), lesquelles permettront ainsi d’entreprendre une étude ciblée de ce phénomène. Enfin, les grottes de Goyet offrent la possibilité de contribuer à la connaissance de la séquence gravettienne du Nord-Ouest européen. Si le long historique des fouilles menées dans ces grottes, ainsi que les mélanges manifestes affectant les collections, ne permettent pas d’envisager une étude approfondie des comportements gravettiens, des observations préliminaires permettent en revanche de s’interroger sur le potentiel de ces sites pour la reconstitution de cette séquence. A l’instar de ce qu’ont montré les travaux récemment conduits sur le Gravettien de Spy (Pesesse & Flas, 2013), il est en effet vraisemblable que les grottes de Goyet aient été occupées à plusieurs moments de la période gravettienne. BODU P., CHEHMANA L., KLARIC L., MEVEL L., SORIANO S., TEYSSANDIER N. (dir.), 2013. Le Paléolithique supérieur ancien de l’Europe du Nord-Ouest : réflexions et synthèses à partir d’un projet collectif de recherche sur le centre et le sud du Bassin parisien, Actes du colloque de Sens, 15-18 avril 2009. Paris : Société préhistorique française (Mémoires de la Société préhistorique française, 56), 516 p. PESESSE D., FLAS D., 2013. Which Gravettians at Spy? In : ROUGIER H., SEMAL P. (eds), Spy Cave. 125 years of multidisciplinary research at the Betche-aux-Roches (Jemeppe-sur-Sambre, Province of Namur, Belgium). Volume I. Bruxelles : Institut royal des Sciences naturelles de Belgique, Société royale belge d'Anthropologie et de Préhistoire, NESPOS Society (Anthropologica et Praehistorica, 123), pp. 257-268. [less ▲]

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See detailTuta absoluta (Lepidoptera: Gelechiidae) ability to develop on potato tubers
Bawin, Thomas ULiege; De Backer, Lara ULiege; Yarou, Boni Barthélémy ULiege et al

Poster (2016, December 16)

Tuta absoluta (Lepidoptera: Gelechiidae) is a widespread pest that threatens commercial production of solanaceous crops. Larvae feed on the mesophyll of all aerial parts of the plants resulting in ... [more ▼]

Tuta absoluta (Lepidoptera: Gelechiidae) is a widespread pest that threatens commercial production of solanaceous crops. Larvae feed on the mesophyll of all aerial parts of the plants resulting in significant yield losses and cosmetic damages.It is classically assumed that underground plant parts are not affected but larval development on potato tubers has recently been suspected. However, this assumption is poorly supported in the literature. Potato tubers might represent an unsuspected mean of persistence and dispersal in agricultural environments.Oviposition tests were here conducted using dormant and active (sprouts less than 5 cm) potato tubers in net cages. Oviposition only occurred on the sprouts of active tubers, supporting larval growth until adult emergency. Tuber dissection revealed burrows starting from sprouts inside the flesh. Additional fitness assays were conducted on non-sprouted tubers and revealed that first instar larvae were able to enter the skin and create burrows, living inside and feeding on potato flesh, although none of them survived. [less ▲]

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See detailEnergy Dissipation in Saturn’s Magnetotail: A Comparative Magnetotail Approach
Yao, Zhonghua ULiege; Coates, Andrew; Ray, Licia et al

Poster (2016, December 16)

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See detailDetermining the Substorm Onset Instability Using Ground and Space-Based Measurements
Rae, Jonathan; Watt, Clare; Murphy, Kyle et al

Poster (2016, December 16)

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Peer Reviewed
See detailFeeding ecology of metamorphic and paedomorphic palmate newts (Lissotriton helveticus) in Larzac
Didaskalou, Emilie; Lejeune, Benjamin ULiege; Lepoint, Gilles ULiege et al

Poster (2016, December 16)

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See detailHead shape disparity of the cod icefishes Trematominae (Notothenioidei, Teleostei)
Frederich, Bruno ULiege; Heindler, Franz M.; Dettai, Agnès et al

Poster (2016, December 16)

The suborder Notothenioidei (Teleostei) has undergone a remarkable adaptive radiation in the Southern Ocean. Within this suborder, the subfamily Trematominae is endemic to Antarctic waters and represents ... [more ▼]

The suborder Notothenioidei (Teleostei) has undergone a remarkable adaptive radiation in the Southern Ocean. Within this suborder, the subfamily Trematominae is endemic to Antarctic waters and represents a dominant component of the shelf fish fauna. After recent advances in molecular phylogenetics, 14 species of Trematomus are currently recognized (including Pagothenia and Cryothenia spp.) comprising both considerable morphological and ecological diversity. Here, we aim to illustrate the main axes of shape variation in Trematomus and explore the evolution of their morphology. A dataset of 96 specimens representing 10 species of Trematomus from the collection of the Natural History Museum of Paris was assembled, and landmark-based geometric morphometrics was applied to quantify head shape disparity. Regular regression analysis revealed significant interspecific allometry while a low percentage of shape variation was explained by size (R2 = 0.11; P < 0.001). Main shape variation across species was explored using a principal component (PC) analysis on shape variables. Two groups diverged along PC1: (1) T. bernacchii, T. hansoni, T. pennellii and T. tokarevi have short cephalic profiles with larger cheeks (lowest values along PC1); and (2) T. lepidorhinus, T. eulepidotus and T. newnesi show lengthened cephalic profiles with larger eyes (highest values along PC1). Trematomus scotti differed from all other species mainly along PC3 indicating more elongated cheeks. Phenogram based on Procrustes shape distances will be compared to molecular phylogenetic trees and morphometric data will be mapped onto phylogenetic trees in order to illustrate the mode of phenotypic diversification of Trematomus during evolution. [less ▲]

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See detailInsects complex associated with the tropical basil, Ocimum gratissimum L. (Lamiaceae) in southern Benin.
Yarou, Boni Barthélémy ULiege; Bokonon-Ganta, H. Aimé; Assogba Komlan, Françoise et al

Poster (2016, December 16)

Tropical basil is an aromatic leafy vegetable used for its medicinal and therapeutic properties in numerous countries in West Africa (Benin, Nigeria, Togo, etc.). In Benin, it is produced on almost all ... [more ▼]

Tropical basil is an aromatic leafy vegetable used for its medicinal and therapeutic properties in numerous countries in West Africa (Benin, Nigeria, Togo, etc.). In Benin, it is produced on almost all urban and periurban garden throughout the year for fresh market. Until now there are few or nearly no publications about the arthropod community of this specie, even less in the context of Benin. Thus, to assess this community, basil plots were mowed using a sweep net in three localities (Ouidah, Togba and Sèmè) of southern Benin. Preliminary results focus on the different insect families that colonized tropical basil in southern Benin environmental conditions. These include: Aphididae (Homoptera), Cercopidae (Homoptera), Chrysomelidae (Coleoptera), Coccinelledidea (Coleoptera), Meloidae (Coleoptera), Braconidae (Hymenoptera), Ichneumonidae (Hymenoptera), Formicidae (Hymenoptera), Vespidae (Hymenoptera), Apoidae (Hymenoptera), Reduviidae (Heteroptera), Pentatomidae (Heteroptera), Pyrgomorphidae (Orthoptera), Acrididae (Orthoptera), Syrphidae (Diptera), Diopsidae (Diptera). Among these families, there are pests: Aphis gossypii G. (Homoptera: Aphididae), Zonocerus variegatus L. (Orthoptera: Pyrgomorphidae), etc.; predators: Ischiodon aegyptius W. (Diptera: Syrphidae), Cheilomenes spp. (Coleoptera: Coccinelledidea), Rhynocoris spp. (Heteroptera: Reduviidae), etc.; parasitoids and pollinators. From this study, it appeared that there is a large diversity of families and functional groups (pests, predators, parasitoids and pollinators) associated with tropical basil . Moreover, the presence of natural enemies could be an advantage for farmers in intercropping systems. This would help reduce the use of synthetic insecticides. This result, which is a first knowledge of the insect fauna associated with tropical basil under the environmental conditions of Southern Benin, will be supplemented by a spatio temporal study to assess the variability and the dynamics of this insect fauna. [less ▲]

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See detailExploring a Mediterranean mesozooplankton 13 year time-series.
Fullgrabe, Lovina; Richir, Jonathan ULiege; Batigny, Antoine et al

Poster (2016, December 16)

Zooplankton plays diverse crucial roles within the marine ecosystem and can also be used as bio- indicator of climate changes since it is very sensitive to environmental changes. Therefore it is essential ... [more ▼]

Zooplankton plays diverse crucial roles within the marine ecosystem and can also be used as bio- indicator of climate changes since it is very sensitive to environmental changes. Therefore it is essential to consider long-term plankton series. Given the analysis of plankton samples is time- consuming, it requires an effective and rapid analytical method. We have used in this work a supervised learning approach adapted for the semi-automatic classification of digital images of the mesozooplankton of the Bay of Calvi (Corsica, France) by using the Zoo/PhytoImage software. Together with a 11-years long zooplankton time-series, a set of nine environmental variables were monitored in order to identify controlling factors and determine whether the communities were sensitive to global environmental changes. The main components of the mesozooplankton community were characterized by both seasonal and inter-annual variability. Additionally, variation of holoplankton and meroplankton differentiated one from each other. The holoplanktonic community could be split into two subgroups according to its variation in function of the environment: cladocerans and appendicularians, and to a lesser extent, copepods on one hand, and cnidarians, chaetognathes and thaliaceans, on the other hand. Regarding inter-annual variation, one year (2007) showed particularly low production of total zooplankton which was also the case for all the different holoplanktonic taxa. Accounting for that phenomenon were identified some potentially underlying environmental factors. Finally, although water temperature increased significantly over the last years along with the frequency of marine heat wave events, no evident change in the global zooplankton composition was observed yet. [less ▲]

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See detailBioaccumulation of trace metals in Mytilus galloprovincialis from the Algerian west coast
Rouabhi, Leila; Rouanne, Hassen Omar; Benkrama, Hadjer et al

Poster (2016, December 16)

Algeria has a 1622 km long coastal strip where a large proportion of the population and the main economic and industrial activities of the country are concentrated. Its coastal fringe therefore suffers ... [more ▼]

Algeria has a 1622 km long coastal strip where a large proportion of the population and the main economic and industrial activities of the country are concentrated. Its coastal fringe therefore suffers from various degradations. In particular, the Bay of Oran is housing industrial, commercial, fishing and recreational activities, where 80 % of domestic and industrial wastewaters are not purified before being discharged into the sea. Thus, the aim of the present work was to evaluate the degree of metallic contamination of coastal waters of western Algeria. Trace metals (Zn, Cu, Ni, Fe and Pb) were measured by atomic absorption spectroscopy in the dry soft tissues (gills and gonads) of the mussel Mytilus galloprovincialis Lamarck, 1819 collected during the four seasons of year 2010 in 2 sites: the highly polluted Oran harbor (S1) and Ain Defla (S2), a site distant enough from Oran and presumed little contaminated. Oran harbor was globally more contaminated than Ain Defla, especially for Fe, Zn and Ni. However, Pb levels were higher at Ain Defla with concentrations up to 3.35 ± 0.25 ppm in the gills. Metal concentrations differed between organs. Fe, Zn and Cu were more accumulated in gills (46.91 ± 1.60 ppm, 25.6 ± 1.07 ppm, 2.68 ± 0.50 ppm, respectively) compared to gonads (29.06 ± 1.07 ppm, 21.76 ± 1.46 ppm ppm, 1.44 ± 0.20 ppm, respectively). Each metal followed a seasonal trend, showing concentration peaks during winter and spring for gills and autumn for gonads. This study demonstrated the need to biomonitor the metallic contamination of Algerian coasts. But such monitoring surveys, relying on organisms, will require consensual sampling and analytical protocols to avoid hazardous conclusions due to tissue speciation and accumulation seasonality. [less ▲]

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See detailCopper toxicity on coral holobiont photosynthetic processes
Georges, Nadège; Richir, Jonathan ULiege; Batigny, Antoine et al

Poster (2016, December 16)

Copper (Cu), an essential micronutrient to organisms, may become toxic when present at too high environmental concentrations. This metal remains an aquatic contaminant of concern, notably because of its ... [more ▼]

Copper (Cu), an essential micronutrient to organisms, may become toxic when present at too high environmental concentrations. This metal remains an aquatic contaminant of concern, notably because of its recent re-use as biocide in metal-based antifouling paints. The aim of this study was to monitor the physiological alterations in a zooxanthellate coral species and its endosymbionts (i.e. the coral holobiont) exposed to increasing Cu concentrations. Nubbins of Seriatopora hystrix were exposed for 8 days in 1 L intermittent respirometers to 5 nominal Cu concentrations: 0-2-5-15-50 ppb. Respirometers were maintained at 25.0±0.2°C with successive open/close cycles of 30 min. A 12/12 hours day-night light regime was applied with constant daylight intensity of 200 μmol photons m-2 s-1. Water renewal rate during the 30 min open cycles was 15 mL.min-1. The photosynthetic performances of coral endosymbionts were assessed daily with a fluorescence imaging system (imaging-PAM). At the end of the 8-days experiment, the maximal photochemical quantum yield (FV/FM) of coral nubbins had decreased by 12% and 38%, respectively, in the 15 ppb and 50 ppb treatments. This decrease was even greater for the effective photochemical quantum yield (ɸPSII) with values dropping by 41% and 54%, respectively. Cu exposure also affected the symbiosis between the coral host and its endosymbionts. Nubbins of the 15 ppb treatment slightly lightened from day 6, whilst nubbins exposed to the 50 ppb treatment lightened from day 3, and started to bleach from day 6. The analysis of nubbins’ primary productivity did not coincide with the above observations, the oxygen production within each respirometer remaining relatively constant during the overall experiment for all treatments. This unexpected observation may be the sign of a compensation mechanism. In conclusion, Cu affected the photosynthetic processes of S. histrix within 8 days from relevant environmental concentrations of 15 ppb. The exposure of corals to toxic chemicals thus has to be considered as an additional stressor to, e.g., ocean acidification or elevated temperature, which may disturb their ecophysiology and lead to bleaching. [less ▲]

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See detailHIPE: Human impacts on ecosystem health and resources of Lake Edward; exploring a poorly known ichthyofaunal
Decru, E; Van Steenberge, M; Bouillon, S et al

Poster (2016, December 16)

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See detailIntegrating Non-Collocated Well and Geophysical Data to Capture Lithological Heterogeneity at a Managed Aquifer Recharge and Recovery Site
Gottschalk, Ian; Hermans, Thomas ULiege; Caers, Jef et al

Poster (2016, December 15)

Aquifer recharge and recovery (ARR) is the process of enhancing natural groundwater resources and recovering water for later use by constructing engineered conveyances. Insufficient understanding of ... [more ▼]

Aquifer recharge and recovery (ARR) is the process of enhancing natural groundwater resources and recovering water for later use by constructing engineered conveyances. Insufficient understanding of lithological heterogeneity at ARR sites often hinders attempts to predict where and how quickly infiltrating water will flow in the subsurface, which can adversely affect the quality and quantity of available water in the ARR site. In this study, we explored the use of electrical resistivity tomography (ERT) to assist in characterizing lithological heterogeneity at an ARR site, so as to incorporate it into a flow and contaminant transport model. In this case, we had non-collocated well core log data and ERT data from a full-scale ARR basin. We compared three independent methods for producing conditional lithology-resistivity probability distributions: 1) a search template to relate the nearest logged well lithologies with ERT resistivity panels, given search criteria; 2) a maximum likelihood estimation (MLE) to match bimodal normal distributions to the histogram of each ERT line; and 3) variogram-based lithology indicator simulations constrained to well data. Each approach leverages Bayes’ Rule to estimate lithology probability given electrical resistivity. The simplest approach (method 1) yields an erroneous conditional probability function where sand dominates the conditional probability at nearly all resistivities, due in part to the strong presence of sand in the wells nearest the ERT lines. The approaches using MLE and lithology simulations (methods 2 and 3) produce similar, more realistic lithofacies probability functions. The range of resistivities where clay and sand overlap differs between methods 2 and 3: ranging between 100 and 200 ohm-m for method 2, and between 30 and 50 ohm-m for the method 3. These differences affect the posterior lithology distributions in multiple point geostatistical (MPS) simulations, and in turn, predictions of flow from models which integrate these results. To test the models, we can compare measured breakthrough times of recharged water at the site to groundwater flow simulation results using the lithofacies models created by each method. The methods described here can inform the integration of non-collocated geophysical data into a variety of applications. [less ▲]

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See detailA step-by-step recipe of band-spli3ng technique for isola8on of ionospheric signal in L-band InSAR data
Furuya, Masato; Suzuki, Takato; De Rauw, Dominique ULiege

Poster (2016, December 15)

Ionospheric signals are widely regarded as nuisance in low- frequency InSAR data, and thus a variety of correction techniques have been proposed. Although band-splitng of range spectrum has been shown to ... [more ▼]

Ionospheric signals are widely regarded as nuisance in low- frequency InSAR data, and thus a variety of correction techniques have been proposed. Although band-splitng of range spectrum has been shown to be effectve (Brcic et al., 2010; Rosen et al., 2010; Gomba et al., 2015), the band- spliting technique seems to have not been widely used. Whereas this would be partly because the impacts of ionosphere on InSAR data are not necessarily serious, we consider that this could be also because the details of the technique have not been reproducibly shown. Here we show our step-by-step recipe of the band-spliting technique, and demonstrate two case studies that reveal the effectiveness of the technique. [less ▲]

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Peer Reviewed
See detailElectrical Resistivity Monitoring of Heat Tracer to Characterize Lab-Scale Hydraulic Conductivity Distributions
Adetokunbo, Peter; Hermans, Thomas ULiege; Oware, Erasmus

Poster (2016, December 14)

Knowledge of the spatial variations of hydraulic conductivity (K) is crucial to almost every hydrogeological investigation. The representative scale of K estimates from traditional slug and pumping tests ... [more ▼]

Knowledge of the spatial variations of hydraulic conductivity (K) is crucial to almost every hydrogeological investigation. The representative scale of K estimates from traditional slug and pumping tests are, however, inadequate to accurately predict hydrogeological processes. There is increasing interest in the application of electrical resistivity tomography (ERT) to quantify spatially continuous K variations. ERT estimation of high-resolution K distributions, however, requires continuous injection of saline tracer (ST) into an aquifer over an extended period, which is feasible but impractical. Here, we present electrical resistivity thermography (ERTh) to evaluate the potential application of time-lapse ER monitoring of heat tracer (HT) to characterize high-resolution K architectures. Unlike ST, long term HT experiments are comparatively easier to manage and repeatable with minimal environmental impact. We estimate K variations via petrophysical coupling of flow and heat transport with joint time-lapse ER and discrete multi-level temperature breakthrough curves. We illustrate the strategy with a 2-D lab-scale sandbox experiment. To construct the heterogeneous field, three lenses with high-K properties with each consisting of gravel, coarse sand, and a mixture of coarse and fine sand, were created within a background of comparatively low-K fine sand. The experiment involved continuous injection and extraction of heat, respectively, at the left and right boundaries of the lab-scale aquifer. We simultaneously performed time lapse ER monitoring of the heat transport and temperature measurements at four discrete multi-levels near the heat extraction well. Results of the coupled inversions demonstrate that ER monitoring of heat tracer provides a unique opportunity to characterize high-resolution spatially continuous K variations, which seems more practical for field applications in contrast to that of the traditional ST. [less ▲]

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See detailThe complex behavior of the satellite footprints at Jupiter: the result of universal processes?
Bonfond, Bertrand ULiege; Grodent, Denis ULiege; Badman, Sarah V. et al

Poster (2016, December 14)

At Jupiter, some auroral emissions are directly related to the electromagnetic interaction between the moons Io, Europa and Ganymede on one hand and the rapidly rotating magnetospheric plasma on the other ... [more ▼]

At Jupiter, some auroral emissions are directly related to the electromagnetic interaction between the moons Io, Europa and Ganymede on one hand and the rapidly rotating magnetospheric plasma on the other hand. Out of the three, the Io footprint is the brightest and the most studied. Present in each hemisphere, it is made of at least three different spots and an extended trailing tail. The variability of the brightness of the spots as well as their relative location has been tentatively explained with a combination of Alfvén waves’ partial reflections on density gradients and bi-directional electron acceleration at high latitude. Should this scenario be correct, then the other footprints should also show the same behavior. Here we show that all footprints are, at least occasionally, made of several spots and they all display a tail. We also show that these spots share many characteristics with those of the Io footprint (i.e. some significant variability on timescales of 2-3 minutes). Additionally, we present some Monte-Carlo simulations indicating that the tails are also due to Alfvén waves electron acceleration rather than quasi-static electron acceleration. Even if some details still need clarification, these observations strengthen the scenario proposed for the Io footprint and thus indicate that these processes are universal. In addition, we will present some early results from Juno-UVS concerning the location and morphology of the footprints during the first low-altitude observations of the polar aurorae. These observations, carried out in previously unexplored longitude ranges, should either confirm or contradict our understanding of the footprints. [less ▲]

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See detailQualité des médicaments antipaludéens et caractéristiques des pharmacies des territoires périurbains de Kinshasa
Mavungu Landu, Don Jethro ULiege; Liégeois, Sophie; Manzambi Kuwekita, Joseph ULiege et al

Poster (2016, December 14)

Contexte: Le paludisme causé par le Plasmodium falciparum demeure un problème majeur de santé publique. Le traitement avec des antipaludiques de bonne qualité est une composante importante dans le ... [more ▼]

Contexte: Le paludisme causé par le Plasmodium falciparum demeure un problème majeur de santé publique. Le traitement avec des antipaludiques de bonne qualité est une composante importante dans le contrôle de cette maladie. Cependant en Afrique centrale, plus de 25% des médicaments serait contrefaits ou de qualité inférieure, situation qui serait encore plus dramatique dans les territoires périurbains. Méthode: Dans le contexte ci-mentionné, une étude préliminaire et prospective a été menée dans la zone de santé de Mont Ngafula 1 située dans les territoires périurbains de la Ville de Kinshasa durant la période allant du 22 février au 17 mars 2016. Treize échantillons de poudre pour suspension d’artéméther et de luméfantrine ont été collectés. L’analyse de la qualité de ces médicaments a été réalisée au moyen de méthodes séparatives génériques utilisant la technique de chromatographie liquide à haute performance couplé à un détecteur à barrettes de diodes. Une caractérisation a été également effectuée dans 127 établissements pharmaceutiques sur base des normes édictées par le Ministère de la Santé Publique congolais. Résultats: Les résultats des analyses des échantillons d’antimalariques (ou antipaludéens) montre que presque la moitié des poudres pour suspension d’artéméther et de luméfantrine ne contenait pas la concentration prévue en artéméther et/ou en luméfantrine. Par ailleurs, le résultat des observations évoque qu’aucun établissement pharmaceutique ne respecte l’ensemble des normes du Ministère de la Santé Publique. [less ▲]

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See detailGeneral chemical composition of almonds (PrunusAmygdalusMiller) grown in eastern Morocco
Houmy, Nadia ULiege; Sindic, Marianne ULiege; Fauconnier, Marie-Laure ULiege et al

Poster (2016, December 14)

The production of almond's kernel has increased continually in eastern Morocco, from 136 000 T in 2012 to 183000 T in 2015. To valorize this kernel, we have analyzed chemical composition of five varieties ... [more ▼]

The production of almond's kernel has increased continually in eastern Morocco, from 136 000 T in 2012 to 183000 T in 2015. To valorize this kernel, we have analyzed chemical composition of five varieties, which were determined as Marcona, Fournat, Ferragnes, Ferraduel and Beldi. Total sugar was ranged between 6,2086 for Marcona and 8,5462 % for Fournat. Total protein was changed from 19,4167 for Ferragnes Ferradul to 22,261 % for Beldi. Total fiber was varied from 15,4197 for Marcona to 18,150 % for Beldi. Ashes was ranged between 2,9079 for Marcona and 3,5606 % for Beldi. Oil content was changed between 51,4741 for Beldi and 56,5688 % for Ferragnes Ferraduel. This large oil content requires us to determine the characteristics of this almond oil. Fatty acid profile was identified, and Oleic acid, Linoleic acid, Palmitic acid and Stearic acid were ranged from 60,76 for Marcona to 69,306 % for Ferragnes Ferraduel, between 20,545 for Ferragnes Ferraduel and 27,45 % for Marcona, between 7,0692 for Ferragnes Ferraduel and 8,010 for Marcona and from 2,009 for Beldi to 2,743 for Marcona, respectively. After all, oxidative stability was mesured to know which oil bears more, and we have concluded that Ferragnes Ferraduel tolerates more of them all. [less ▲]

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See detailLandslide characterization from space in the Rift flanks west of Lake Kivu (DRC)
Monsieurs, Elise ULiege

Poster (2016, December 13)

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See detailEcosystem services in Wallonie ... What's up?
Maebe, Laura ULiege; Pipart, Nathalie; Dendoncker, Nicolas et al

Poster (2016, December 13)

Detailed reference viewed: 15 (1 ULiège)
See detailSolar electrons observed in the magnetosphere
Shi, Quanqi; Wang, linghua; Zong, Qiugang et al

Poster (2016, December 12)

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See detailRenverser la machine: de la technique comme outil d’exploitation à la technique comme moyen de régénération environnementale: l’approche paysagère comme préalable à la réhabilitation des sites industriels désaffectés dans la Province de Liège.
Baldin, Elisa ULiege

Poster (2016, December 12)

A partir de l’interaction entre pensée architecturale et dynamique de l’environnement, la recherche touche les problématiques de régénération des sites industriels désaffectés du bassin liégeois en ... [more ▼]

A partir de l’interaction entre pensée architecturale et dynamique de l’environnement, la recherche touche les problématiques de régénération des sites industriels désaffectés du bassin liégeois en mettant à l’épreuve le principe de « recyclage » architectural et paysager. Le site Espérance Longdoz, à Seraing, représente, en tant qu’objet de mon travail de fin d’études, un cas spécifique mais indicatif d’un phénomène territoriale, qui concerne la Vallée de la Meuse. Le projet d’aménagement urbain, où le re-dessin de l’espace se fonde sur une re-interprétation des traces existantes, ouvre à une réflexion sur la complémentarité des éléments artificiels et des éléments naturels dans le projet de régénération urbaine. L’approche paysagère intégrée est proposée comme outil pour activer des nouveaux cycles de vie, selon une dynamique écologique fondée sur la continuité à trouver entre ressource et déchet: une alternative à l’abandon et à la disparition des lieux qui ont construit l’histoire sociale et l’identité du territoire. Lectures et observations des mutations morphologiques, complétées par des analyses comparatives entre expériences locales et européennes similaires concernant des réalisations, projets en cours et acteurs engagés, fondent une réflexion critique centrée sur le renversement du sens de la machine: autrefois celle qui a exploité et bouleversé les équilibres de la terre et aujourd’hui à l’opposé, celle qui se développe et transforme les milieux à travers temporalités et actions naturelles. La thèse explore les technologies soft, de phyto-dépollution ou autre procédés de transformation naturelle, à réinsérer dans le processus de mutation de nos milieux de vie comme un système de structures d’assainissement environnemental et comme un ensemble de stratégies de reconstitution d’un paysage productif urbain/périurbain, capables de réactiver des moyens soutenables aptes à « renverser la machine » d’exploitation industrielle. [less ▲]

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See detailSmart Cities. Ingénierie des usages et co-conception : un nouveau cadre pour le développement durable des bâtiments et villes intelligents.
Schelings, Clémentine ULiege; Elsen, Catherine ULiege

Poster (2016, December 12)

Ce projet de recherche aborde le défi de l'opposition des utilisateurs finaux à certains des concepts clés de la ville intelligente (Smart City), et plus spécifiquement leur faible niveau d'engagement vis ... [more ▼]

Ce projet de recherche aborde le défi de l'opposition des utilisateurs finaux à certains des concepts clés de la ville intelligente (Smart City), et plus spécifiquement leur faible niveau d'engagement vis-à-vis du développement de leurs bâtiments intelligents. L'acceptation des utilisateurs finaux ayant une influence déterminante sur le taux d'échec / de réussite du projet Smart City en tant que tel, cette recherche se fonde sur l'expertise des concepteurs de la Smart City (architectes, ingénieurs architectes; ingénieurs en construction, ingénieurs en développement urbain et environnement, ...) et suggère qu'une partie de la solution relève de l'interaction que ces acteurs peuvent avoir avec les utilisateurs finaux au cours des phases préliminaires du processus de construction d'un projet. La recherche vise à développer des cadres théoriques dédiés (i) à l'ingénierie des usages et (ii) au processus de co-conception, de manière à outiller les concepteurs avec des modèles théoriques, des méthodes de travail et des outils adaptés pour (i) la capture et l'analyse des comportements des utilisateurs finaux et (ii) l'investissement actif des utilisateurs finaux au sein des processus de conception de bâtiments intelligents et de leurs modèles d'occupation intelligente. [less ▲]

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See detailXenogeneic graft-versus-host disease: Impact of Th17 cells.
Delens, Loïc ULiege; SERVAIS, Sophie ULiege; Vrancken, Louise et al

Poster (2016, December 08)

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See detailFACILITER L'ADHÉRENCE AU TRAITEMENT IMMUNOSUPPRESSEUR CHEZ LES TRANSPLANTÉS DU REIN- TEST D'UN SYSTEME DE COACHING PAR SMARTPHONE
Saint-Remy, Annie ULiege; Spiroux, Marie; WEEKERS, Laurent ULiege et al

Poster (2016, December 08)

Facilitate adherence to immunosuppressive treatment in kidney transplant recipients – test of a coaching system with smartphone application A.Saint-Remy, M. Spiroux, L. Weekers, C. Bonvoisin, JM ... [more ▼]

Facilitate adherence to immunosuppressive treatment in kidney transplant recipients – test of a coaching system with smartphone application A.Saint-Remy, M. Spiroux, L. Weekers, C. Bonvoisin, JM. Krzesinski Introduction: Nonadherence to immunosuppressants is a determining cause of graft loss. The present study tested during 1 month, the usefulness and the effectiveness of a coaching system using smartphone application (the Transplant Smartcoach®) on adherence in kidney transplant recipients. Methodology: The sample included 51 patients (28m/23w) transplanted for 1 year at least, mean age 52±12 years, mean graft survival 3.7±1 years. Each patient had a smartphone configured with its detailed treatment. Using the smartphone application, patient had to notify daily the intake of each tablet into a 2-hour window. If no notification was performed 1 hour later than the scheduled time of intake, the patient was contacted by a nurse to remind him to take medications. Results: 90 % of patients were treated with Tacrolimus and 10 % with cyclosporine, associated with mycophenolic acid (41 %) or mycophenolate mofetil (49 %), 41 % had corticosteroids. Whatever was the immunosuppressant, a perfect adherence (medications taken at the scheduled time) was observed on average in 53 % of the morning monthly intakes. There was no difference in adherence rates between the morning intakes of Advagraf ® (once/day) and Prograft® (2 times/day), a decrease of perfect adherence was identified with the evening intake of Prograft® (53 % vs 44%; P=0.07) and the one of Myfortic® (P=0.03) with consequently an increased frequency of nurse’s recalls. Adherence was lower in younger patients and in those still working. The complexity of treatment (many drugs intake/day) and the respect for time intervals between drug intakes were the major barriers to adherence. When compared to the one measured before using the Smartcoach, the variability (coefficient of variation, %) of the Tacrolimus trough level decreased by 32.6 % (P=0.027) in the 3 to 6 months following the test. Conclusion: patients appreciated the ease and usefulness of the coaching system with smartphone application to help medication adherence. Coupled with therapeutic education of the patients, that tool deserves to be used notably in newly transplanted patients and when a worrying decrease in adherence is observed to help them in the management of a rigorous adherence which should contribute to graft survival. [less ▲]

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULiege; Mendoza Gallego, Carlos ULiege; Lismont, Marjorie ULiege et al

Poster (2016, December 08)

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side ... [more ▼]

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side-reactions or product degradation due to overexposure. The recent implementation of photochemical processes in microreactors under continuous-flow conditions appeared to be much more powerful than its batch analogue in terms of irradiation efficiency and light penetration. Furthermore, the fine control of residence time ensures an accurate control of the irradiation time, avoiding side-reactions and degradation. The photooxidation of (L)-methionine with singlet oxygen using Rose Bengal as a sensitizer was successfully implemented in a microreactor setup and led to total and selective conversion into methionine sulfoxide, an important building block for the organic synthesis of peptides or functionalized amino acid. The reaction was performed in 2 min while the same reaction in batch took 2h. [less ▲]

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