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See detailInterpretatie van het EVRM: 'nationale veiligheid'
Notermans, Pierre ULiege

Poster (2018, December 14)

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See detailTailored films and biomaterials: another reality
Mahy, Julien ULiege; Wolfs, Cédric ULiege; Tilkin, Rémi ULiege et al

Poster (2018, December 10)

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See detailSurface modification of biodegradable microcarriers and their characterization by microscopy
Rocca, Coralie ULiege; Sevrin, Chantal ULiege; Grysan, Patrick et al

Poster (2018, December 10)

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See detailCosmology with type Ia supernovae : the ultraviolet 'catastroph'? Evolution with redshift of type Ia supernovae in the ultraviolet domain
Hauret, Clémentine ULiege; Magain, Pierre ULiege

Poster (2018, December 07)

To extract useful cosmological information from type Ia supernovae (SNe Ia), standardization laws have to be determined and applied assuming that these objects do not intrinsically change with redshift ... [more ▼]

To extract useful cosmological information from type Ia supernovae (SNe Ia), standardization laws have to be determined and applied assuming that these objects do not intrinsically change with redshift. Recently, to improve that standardization, attention has been drawn towards the SNe Ia UV spectra. This resulted in (what seemed as) the discovery of two subpopulations of objects, grouped by their bluer or redder UV colors. To confirm the existence of these subpopulations, we significantly enlarged the number of studied SNe Ia (going from ~100 to ~700 objects). With such a sample, SNe Ia cannot be separated in two groups anymore but they rather follow a continuum of colors. More critically, we showed that the SNe Ia UV colors display a significant evolution with redshift, resulting in a potentially important impact on subsequent cosmological measurements. [less ▲]

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See detailTrophic strategies of reef-building corals under contrasted environmental conditions of East Asian Seas
Sturaro, Nicolas ULiege; Hsieh, Yun Li Eric; Liu, Ling-Wen et al

Poster (2018, December 07)

Most reef-building corals can derive nutrition either autotrophically or heterotrophically, which allows them to use diverse trophic pathways (HOULBRÈQUE & FERRIER-PAGÈS, 2009; MUSCATINE, 1980). Therefore ... [more ▼]

Most reef-building corals can derive nutrition either autotrophically or heterotrophically, which allows them to use diverse trophic pathways (HOULBRÈQUE & FERRIER-PAGÈS, 2009; MUSCATINE, 1980). Therefore, when facing environmental changes, these organisms are expected to demonstrate an intrinsic ability to acclimatise through trophic plasticity. Despite the ecological importance of these corals, our understanding of their trophic strategies is currently impaired by a lack of rigorous research approaches; a failure to consider the intraspecific variability of coral species and an oversimplification of the proxies of heterotrophic habits (e.g. corallite diameter) (MADIN et al., 2016). In order to understand how trophic plasticity could allow them to acclimatise, this study proposed to assess the trophic plasticity of morphologically contrasted coral species (e.g. Stylophora pistillata, Porites sp, Isopora palifera). We determined the stable isotope ratios of carbon and nitrogen in the coral host tissues and algal symbionts and compared these in corals inhabiting areas around Taiwan characterised by contrasted temperature (from high to low latitudes) and light levels (from shallow to mesophotic waters). For each area, we evaluated the intraspecific trophic variability by estimating and comparing coral isotopic niches as a proxy for trophic niches. Our results on Stylophora pistillata revealed no overlap of the isotopic niches for the host and symbiont from different locations (unpublished data), suggesting that these coral colonies are supported by different core resources. Moreover, the isotopic niche of higher latitude coral colonies was larger than those from the lower latitudes (unpublished data), highlighting a certain trophic plasticity that may be related to more variable environmental conditions in the higher latitudes. Analyses of additional species and locations will provide essential insights into the trophic strategies of reef-building corals and how these species might adjust their nutrition in response to environmental changes. [less ▲]

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See detailMethodology for the design of climate responsive houses for optimized thermal comfort in Quetta, Pakistan
Mahar, Waqas Ahmed ULiege; Attia, Shady ULiege

Poster (2018, December 07)

A building must be energy-efficient and provide comfortable indoor environment to the residents. The Building Energy Code of Pakistan (BECP) only focuses on commercial buildings. In today’s scenario a ... [more ▼]

A building must be energy-efficient and provide comfortable indoor environment to the residents. The Building Energy Code of Pakistan (BECP) only focuses on commercial buildings. In today’s scenario a standard must include the context and climate considerations. The aim of this study is to improve indoor thermal comfort of free-running houses in Quetta, Pakistan and raise the awareness of builders about climate sensitivity. [less ▲]

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See detailThe M@dnote project. Giving colour to Grey Literature in General Practice. A collaborative GPs knowledge network
Pizzanelli, Miguel; Resnick, Melissa P.; Cardillo, Elena et al

Poster (2018, December 03)

Abstract (397 words) “M@dNotes Project”. Giving colour to Grey Literature in General Practice. A collaborative GPs knowledge network. Sharing the results of research and scientific production is crucial ... [more ▼]

Abstract (397 words) “M@dNotes Project”. Giving colour to Grey Literature in General Practice. A collaborative GPs knowledge network. Sharing the results of research and scientific production is crucial for the survival of all disciplines. Health information is becoming uncertain and powerful economic interests disrupting medical information has triggered a loss of credibility. M@dNotes project (in Spanish NotasL@cas), is the by-product of non-profit collaborative international knowledge network of general practitioners, family physicians, and healthcare workers. One of the aims of this network is to discuss the validity of the information available on sensitive subjects and build a knowledge exchange network inside a community of practice. The project was born because we realized that more than 95% of the postgraduate and pregraduate research/reports/ scholar works (monographic works) to accomplish or finished one part of the training were finally lost and unpublished. Most of them come from interesting "minds" and are interesting topics to improve the applying of evidence in medical healthcare. From this perspective, M@dNotes is a unique local platform to rescue knowledge and support exchange and collaboration. Perhaps is isolated due to the circumstance of being restricted to few countries or a small group of practice. Nevertheless, we are trying to improve our qualifications in order to link M@dNotes to an open access network. It has been created to share different materials excluded from traditional academic publishing and commercial distribution channels: monographic, commented articles, files with references searches. These materials considered “grey literature” are the product of intense learning process and has been produced to achieve a qualification, be presented in a conference or were collected by users spontaneously through the web searches, with a knowledge purpose. Many of them qualified with an appropriate level because were submitted to a peer review or judged by a scientific committee to get an approval. The project includes an indexing method (Q Codes and ICPC) to manage and retrieve the materials received. Q-Codes are intended for indexing Family medicine related documentation jointly with the International Classification of Primary Care (ICPC). The project provides a particular folder to upload and share the documents. A web blog is the dissemination tool or friendly interface to share and retrieve the documents shared in the network. Through a facility in the cloud, it is possible to access an excel file with all the materials shared, codes assigned and the hyperlink to get a pdf file of each material. [less ▲]

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See detailAssessment of mammal biodiversity and bushmeat offtake in the tropical forests of southeastern Cameroon
Lhoest, Simon ULiege; Fonteyn, Davy ULiege; Hette, Samuel et al

Poster (2018, November 27)

Tropical forests of central Africa host an important part of the world’s terrestrial biodiversity and provide numerous provisioning, regulating, and cultural ecosystem services to human populations. Major ... [more ▼]

Tropical forests of central Africa host an important part of the world’s terrestrial biodiversity and provide numerous provisioning, regulating, and cultural ecosystem services to human populations. Major threats hang over those diverse ecosystems, namely land use changes and consumption of bushmeat. Our study aimed to assess mammal diversity and bushmeat consumption in three contrasted and largely represented forest land allocation types in southeastern Cameroon: (i) a protected area, (ii) a FSC-certified logging concession, and (iii) three community forests. Mammal inventories were conducted with 44 camera traps installed for 3 months. Bushmeat consumption was quantified using both tracking of volunteer hunters over 651 kilometers and the daily monitoring of the food bowl of 55 households for 3 months. Though a great part of the mammal diversity is retained inside the logging concession, the protected area holds the richest and most abundant mammal communities, whereas community forests were found to be defaunated and structurally disturbed. The size of the hunting territories is influenced by many factors such as human population density or the presence of alternative protein sources. Although poaching controls in the protected area and, to a lesser extent, in the certified logging concession appear to play a deterrent role, evidence of hunting activities were found in all land allocation types. Bushmeat represents on average 56% of the animal protein consumed by households, the remaining part being mainly fish. Our results demonstrated the ability of the certified logging concession and the protected area in the conservation of wildlife species and the provision of bushmeat for local populations. It remains essential to maintain and develop anti-poaching patrols in those areas, strategically based on geographic data of hunting pressure. Current levels of hunting activities also confirm the need for the development of alternatives to bushmeat. [less ▲]

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See detailGestion de la reproduction des Faux bourdons par les apiculteurs de la Wilaya de Tizi-Ouzou
Yahi, Krimou; Kaidi, Rachid; Touazi, Leghel et al

Poster (2018, November 26)

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See detailCaractérisation de l'apiculture dans la Wilaya de Sétif
Touazi, Leghel; Yahi, Krimou; Moula, Nassim ULiege

Poster (2018, November 26)

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See detailElectrochemical mechanism and effect of carbon addition during hydrothermal synthesis to improve the electrochemical performance of Fe1.19(PO4)(OH)0.57(H2O)0.43 cathode material for Li-ion batteries
Mahmoud, Abdelfattah ULiege; karegeya, Claude; Sougrati, Moulay Tahar et al

Poster (2018, November 25)

Since the introduction of lithium-ion batteries (LIBs) to market in 1991, their performance has improved significantly, which has been achievable through development in materials technologies. However ... [more ▼]

Since the introduction of lithium-ion batteries (LIBs) to market in 1991, their performance has improved significantly, which has been achievable through development in materials technologies. However, further breakthroughs are still needed to ameliorate cycle-life, safety and energy density of LIBs. This requires new electrode materials and a detailed understanding of the electrochemical mechanisms during cycling. Transition metal phosphates are interesting candidates as cathode materials for LIBs [1]. In this work, we report the electrochemical performance of FPHH/C and FPHH/CNT composites where FPHH represents Fe1.19(PO4)(OH)0.57(H2O)0.43 while carbon black and carbon nanotubes (CNT) were used as precursors in the one-pot hydrothermal synthesis, respectively. We show that the addition of conducting carbon black into the solution has a strong influence on reducing the particle size and tailoring their morphology, but does not interfere with the formation of the FPHH phase. Thanks to its favorable microstructural characteristics, the FPHH-10 wt% C and FPHH-20 wt% C materials exhibited good performance [2]. The CNT also improve the performance of FPHH such as capacity retention (500 cycles at 1 C). The mechanisms of lithiation-delithiation were investigated by combining operando X-ray diffraction and 57Fe Mössbauer spectroscopy. FPHH undergoes a monophasic reaction based on Fe3+/Fe2+ redox process. However, the variations of the lattice parameters and 57Fe quadrupole splitting indicate a more complex mechanism than a random occupation of the vacant sites within FPHH. This can be related to the peculiar structure of FPHH formed by chains of face sharing (Fe0.6•0.4)O6 octahedra connected by PO4 tetrahedra and by channels for Li diffusion along [100] and [010] directions. The existence of Fe vacancies provide interconnections between the one-dimensional channels, improving lithium diffusion within FPHH. This mechanism, combined with the addition carbon black or nanotubes in the solution prior to hydrothermal treatment as a simple and effective way to reduce particle size and improve electronic conductivity, provides good cycle life and rate capability for FPHH. Acknowledgements A. Mahmoud is grateful to University of Liege and FRS-FNRS for the grants and thanks to the Walloon region for a Beware Fellowship Academia 2015-1, RESIBAT n° 1510399. Part of this work was supported by the Walloon Region under the “PE PlanMarshall2.vert” program (BATWAL – 1318146). References 1. C. Karegeya, A. Mahmoud, F. Hatert, B. Vertruyen, R. Cloots, P.E. Lippens, F. Boschini, Journal of Power Sources 388 (2018) 57-64. 2. C. Karegeya, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschini, Electrochim. Acta 250 (2017) 49-58. [less ▲]

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See detailSpray drying synthesis of Na3V2(PO4)2F3/C cathode material for Na-ion batteries: study of the effect of the carbon source on electrochemical performance
Eshraghi, Nicolas ULiege; Bodart, Jérôme ULiege; Mahmoud, Abdelfattah ULiege et al

Poster (2018, November 25)

Lithium-ion batteries have enjoyed great success and have outperformed other rechargeable battery system since 1980. However, Li-ion batteries face many challenges and limitations: safety, the low ... [more ▼]

Lithium-ion batteries have enjoyed great success and have outperformed other rechargeable battery system since 1980. However, Li-ion batteries face many challenges and limitations: safety, the low abundance of lithium in the Earth’s crust. Recently, Sodium-ion batteries attracted a lot of interest as a potential alternative to lithium-ion batteries for large-scale energy storage applications, due to the large natural abundance and lower cost of sodium. In recent years, fluorophosphates with the NASICON (Na Super-Ionic Conductor) type structure are considered among the most interesting series of cathode materials for Li/Na-ion batteries, because they exhibit rich chemistry, attractive lithium/sodium insertion properties and thus offer promising electrochemical properties [1]. Na3V2(PO4)2F3 (NVPF) attracted high attention thanks to its promising electrochemical properties. The inductive effects of both PO43- and F- allow for a high working potential combined with a high theoretical specific capacity due to the multiple oxidation states of vanadium[1-2]. One of the key drawbacks of Na3V2(PO4)2F3 electrodes is their low intrinsic electronic conductivity. NVPF and NVPF/carbon composite materials were prepared by spray-drying method using the same conditions used in our previous work [2]. Spray drying is a cost-effective and easily up-scalable route to prepare homogeneous multi-component powders, thus making it a suitable method to incorporate carbon in the composite powder. We used different carbon sources like conductive carbons (MWCNTs, Carbon Black, etc) and organic sources (PVA, Citric Acid, Ascorbic acid, etc) to prepare NVPF/carbon composite powders. the structural, electrochemical, and morphological properties of the synthesized Na3V2(PO4)2F3/C samples were systematically investigated in order to understand the influence of carbon source on structural and morphological properties and most importantly electrochemical performance of NVPF and NVPF/carbon composite cathode materials for Na-ion batteries. The chemical diffusion of Na ions was studied using results obtained by varying scan rates in cyclic voltammetry measurements. Raman spectroscopy is used to evaluate the quality in disordered carbon materials and its electronic conductivity [3] and compared the results with the results from EIS and cycling performance of different samples. Acknowledgments The authors are grateful to University of Liege and FRS-FNRS for grants. Part of this work was supported by the Walloon Region under the “PE PlanMarshall2.vert” program (BATWAL – 1318146). A. Mahmoud thanks to the Walloon region for a Beware Fellowship Academia 2015-1, RESIBAT n° 1510399. References: [1] R. A. Shakoor, D-H. Seo, H. Kim, Y-U. Park, J. Kim, S-W. Kim, H. Gwon, S. Leec and K. Kang, Mater. Chem. 22 (2012) 20535–20541. [2] N. Eshraghi, S. Caes, A. Mahmoud, R. Cloots, B. Vertruyen, F. Boschin, Electrochimica Acta, 228, (2017), 319-324. [3] A. Mahmoud, S. Caes, M. Brisbois, R. P. Hermann, L. Berardo, A. Schrijnemakers, C. Malherbe, G. Eppe, R. Cloots, B. Vertruyen, F. Boschin J Solid State Electrochem. 22 (2018) 103-112. [less ▲]

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See detailEtude des liens entre l’activation comportementale, l’évitement comportemental et les symptômes de la dépression en fonction du genre : Une étude de réplication.
Krings, Audrey ULiege; Wagener, Aurélie ULiege; Blairy, Sylvie ULiege

Poster (2018, November 17)

INTRODUCTION: La dépression est un trouble très prévalent dont les conséquences sociétales en termes économiques, sociales et personnelles sont importantes (World Health Organization, 2017). Cette ... [more ▼]

INTRODUCTION: La dépression est un trouble très prévalent dont les conséquences sociétales en termes économiques, sociales et personnelles sont importantes (World Health Organization, 2017). Cette pathologie est associée à des différences de genre (Parker & Brotchie, 2010). Wagener et al. (2016) ont notamment montré des différences de genre dans les profils de symptômes rapportés (e.g., des scores plus élevés de tristesse, d'auto-critique chez les femmes, des scores plus élevés d'échecs passés et de perte de plaisir chez les hommes). Dans leur étude, les auteurs se sont également intéressés aux liens entre d’une part l’activation comportementale et l’évitement comportemental et d’autre part la présence d’une symptomatologie dépressive et ce, en tenant compte du genre. Leurs résultats suggèrent que l'évitement comportemental prédit positivement presque tous les symptômes dépressifs chez les femmes et les hommes tandis que l'activation comportementale prédit négativement presque tous les symptômes des deux genres. Notre objectif a été de répliquer les résultats de cette étude. MÉTHODE : 260 sujets tout-venants (165 femmes, 95 hommes) ont complété une échelle évaluant la symptomatologie dépressive (BDI-II) ainsi qu’une échelle évaluant l’activation comportementale et l’évitement comportemental (BADS-SF). RÉSULTATS: Comme dans l’étude initiale, les femmes rapportent des scores plus élevés de tristesse, d’indécision, d’augmentation de l’appétit et de perte d’intérêt pour le sexe. Cependant, nos résultats ne suggèrent pas des scores plus élevés pour les échecs passés et la perte de plaisir chez les hommes. Dans notre échantillon, l'évitement comportemental prédit positivement une majorité de symptômes dépressifs chez les femmes et les hommes, tandis que l'activation comportementale prédit négativement un ensemble de symptômes, mais pas une majorité. CONCLUSIONS: Les résultats seront discutés par rapport à l’étude initiale et aux implications cliniques actuelles: (1) pour souligner la pertinence de la combinaison d'outils d'évaluation spécifiques au genre; (2) souligner le besoin d'une intervention psychologique sur mesure. [less ▲]

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See detailComparison of three automated strip analyzers to Cobas 800 for the analysis of glucose, proteins, albumin and creatinine
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine glucose, proteins, albumin and creatinine are measured for the screening of diabetes and renal diseases. The automated strip analyzers are used for quick screening of large populations. The goal of ... [more ▼]

Urine glucose, proteins, albumin and creatinine are measured for the screening of diabetes and renal diseases. The automated strip analyzers are used for quick screening of large populations. The goal of this study was to compare a more accurate method, the Cobas 8000 (Roche, Bale, Switzerland) to three automated analyzers: the Clinitek Novus (Siemens, Munich, Germany), the UC-3500 (Sysmex, Kobe, Japan) and the AutionMax (Menarini, Florence, Italy). A total of 284 urine samples were prospectively collected for the comparison. The samples were analyzed on the three analyzers within 2 hours. Before the analysis of samples by each method, an aliquot was frozen at -80°C. All samples were then defrost and analyzed in one batch with the Cobas 8000 (Roche, Bale, Switzerland) within the month. The diagnostic accuracy was determined thanks to the results of the Cobas 8000. However, the creatinine and albumin cannot be assessed with the AutionMax. [less ▲]

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See detailUrine sediment analysis: comparison of three automated analyzers to manual microscopy
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine microscopic analysis is an old method that reveals information about kidney health. Several automated analyzers, that are less time-consuming, are currently available, but manual microscopy is still ... [more ▼]

Urine microscopic analysis is an old method that reveals information about kidney health. Several automated analyzers, that are less time-consuming, are currently available, but manual microscopy is still the gold-standard method. The goal of this study was to validate and compare three automated analyzers to manual microscopy: the Atellica UAS800 (Siemens, Munich, Germany), the UF-4000 (Sysmex, Kobe, Japan) and the SediMax (Menarini, Florence, Italy). We first validated each analyzer. A total of 359 samples were analyzed with the three analyzers and with a manual microscope within 2 hours. Two trained reviewers used a microscope with bright field, contrast phase and polarized light to identify urine elements. The diagnostic accuracy was determined thanks to microscopy results. [less ▲]

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See detailDiagnostic accuracy of three automated urine analyzers compared to urine culture
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine culture is an important diagnosis method of urinary tract infection. However, it is time-consuming and results aren’t available quickly. Automated urine analyzers have been developed to screen urine ... [more ▼]

Urine culture is an important diagnosis method of urinary tract infection. However, it is time-consuming and results aren’t available quickly. Automated urine analyzers have been developed to screen urine samples more rapidly. The goal of this study was to compare three automated analyzers to urine culture: the Atellica UAS 800 (Siemens, Munich, Germany), the UF-4000 (Sysmex, Kobe, Japan) and the SediMAX (Menarini, Florence, Italy). We first validated each analyzer. We analyzed then 318 samples with the three analyzers within 2 hours after sample reception. An urine aliquot was collected before sediment analysis for bacteria culture. Ten microliters of un-centrifuged urine was inoculated on blood agar and CLED agar plates, then they were incubated aerobically at 36°C for 24 h. Bacteria count of each analyzer was compared to urine culture to determine diagnostic accuracy. The result was considered positive when the bacteria growth reached 104 CFU/ml. We also used the results of leukocytes and nitrites results from the strip measurement to improve the accuracy. The abilities of the UF-4000 to distinguish Gram positive (GP) from Gram negative (GN) bacteria, and of the UAS 800 to identify rod and cocci, were determined. [less ▲]

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See detailDo Older Adults' Growth Mindsets Predict Their Willingness to Engage in Preventative Health Behaviors?
Marquet, Manon ULiege; Chasteen, Alison L; Plaks, Jason E et al

Poster (2018, November 15)

Promoting health behaviors among older adults has become increasingly important given the accumulating health problems that can accompany older age. The present study examined the linkages between ... [more ▼]

Promoting health behaviors among older adults has become increasingly important given the accumulating health problems that can accompany older age. The present study examined the linkages between implicit theories regarding the modifiability of abilities, health behaviors, and subjective age among older adults. A total of 147 older adults (Mean age = 68.62, SD = 5.40) completed, among other measures, questions assessing their implicit theories of ability and their subjective age. They also reported their perceptions of the benefits of different health behaviors (i.e., healthy sleep and diet habits, exercising, and seeking help for hearing or vision problems) for slowing the effects of aging as well as their intentions to engage in those behaviors. As hypothesized, we found that the more older people believed that abilities are changeable, the more they perceived benefits of health behaviors, which in turn increased their intentions. Also, the greater their intentions, the younger the subjective age they reported. The finding that perceived benefits mediated the relationship between implicit theories and intentions toward health behaviors may have important implications for designing interventions to promote and maintain an active and healthy lifestyle in later life. Interventions that focus on modifying fixed mindsets regarding general abilities could indirectly yield positive consequences on intentions to engage in health behaviors by leading older people to see those behaviors as useful for slowing the effects of aging. [less ▲]

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See detailEstetrol, a newly designated orphan drug, for attenuation of neonatal Hypoxic-Ischemic Encephalopathy (HIE)
Tskitishvili, Ekaterine ULiege; Pequeux, Christel ULiege; VIELLEVOYE, Renaud ULiege et al

Poster (2018, November 13)

Estetrol, a newly designated orphan drug, for attenuation of neonatal Hypoxic-Ischemic Encephalopathy (HIE) Ekaterine Tskitishvili1*, Christel Pequeux1, Renaud Viellevoye2, Michelle Nisolle3, Agnes Noël1 ... [more ▼]

Estetrol, a newly designated orphan drug, for attenuation of neonatal Hypoxic-Ischemic Encephalopathy (HIE) Ekaterine Tskitishvili1*, Christel Pequeux1, Renaud Viellevoye2, Michelle Nisolle3, Agnes Noël1 and Jean-Michel Foidart1 1Laboratory of Development Biology and Tumor, University of Liege, Liege, Belgium; 2Department of Pediatrics, University of Liege, Liege, Belgium; 3Department of Ob/Gyn, University of Liege, Liege, Belgium Estetrol (E4) is a fetal estrogen synthesized only during human pregnancy. We aimed to study its role in attenuation of neonatal HIE. In vitro we defined antioxidative and cell viability effects of E4 on primary hippocampal cell cultures in oxidative stress condition by using lactate dehydrogenase (LDH) activity and cell survival (MTS) assays. To study the neuroprotective and therapeutic effects of E4 in vivo neonatal HIE model of 7-day-old newborn rat pups was used. Brains were studied at the level of the hippocampus and cortex. Intact cell counting and expressions of markers for gray and white matter (MAP-2 and MBP), neurogenesis (DCX) and angiogenesis (VEGF) were evaluated by histo- and immunohistochemistry. The serum levels of brain damage markers (S100B and GFAP) were measured by ELISA. Our results demonstrate that E4 has significant antioxidative and cell survival properties along with neuroprotective and therapeutic effects. It decreases the early gray and white matter loss and promotes neuro- and angiogenesis in vivo. Combined use of E4 with other steroids does not have priority over the single use of E4. We also defined that E4's antioxidative actions mostly depend on ERα and ERβ, whereas neurogenesis and possibly promyelinating activities might be realized through ERβ. Treatment by E4 has no effects on body weight, brain weight or body temperature. E4 might become an important safe and physiological substance to treat neonatal HIE. Based on our data EMA granted orphan drug designation to E4. References 1.Tskitishvili E, Nisolle M, Munaut C, Pequeux C, Gerard C, Noel A, Foidart JM.Estetrol attenuates Neonatal Hypoxic-Ischemic brain injury. Exp Neurol 2014; 261:298-307. 2. Tskitishvili E , Pequeux C, Munaut C, Viellevoye R, Nisolle M, Noel A, Foidart JM. Use of Estetrol with other Steroids for Attenuation of Neonatal Hypoxic-Ischemic brain injury: to combine or not to combine? Oncotarget 2016; 7(23):33722-43. 3. Tskitishvili E,Viellevoye R, Gerard C, Pequeux C, Munaut C, Nisolle M, Noel A, Foidart JM. Neonatal Hypoxic-Ischemic Encephalopathy: a new view of an old problem. Références en Gynécologie Obstetrique. 2016 17;1-4 4. Tskitishvili E, Pequeux C, Munaut C, Viellevoye R, Nisolle M, Noël A, Foidart JM. Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study. J Endocrinol. 2017; 232(1):85-95. 5.Foidart JM, Tskitishvili E. International patent application. Estrogenic components for use in the treatment of neurological disorders. [less ▲]

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See detailOperando Mössbauer study of Fe4+/Fe3+ redox couple in NaFeO2
Sougrati, Moulay-Tahar; Stievano, Lorenzo; Darwiche, Ali et al

Poster (2018, November 12)

Among the potential positive electrode materials for Na-ion batteries, iron-based compounds have been regarded as promising candidates for the reversible (de)intercalation of Na on the basis of their high ... [more ▼]

Among the potential positive electrode materials for Na-ion batteries, iron-based compounds have been regarded as promising candidates for the reversible (de)intercalation of Na on the basis of their high abundance in the Earth’s crust and their low environmental impact. The model oxide O3-type NaFeO2, synthesized via the simple reaction of iron oxide and Na2CO3 at 600°C, has been identified as the most interesting one from the viewpoint of both gravimetric and volumetric energy density.[1–3] Na/NaFeO2 cells cycle through a relatively flat potential plateau between 3.3 and 3.4 V vs. Na+/Na, commonly linked with the Fe4+/Fe3+ redox couple. However, if cycling is extended above 3.5 V, other irreversible reaction plateaus appear, leading to the material degradation. 57Fe Mössbauer spectroscopy is thus a method of choice for the study of both (1) the cycling mechanism and (2) the irreversible reactions occurring above 3.5 V. In this work, operando 57Fe Mössbauer analysis was carried out during the electrochemical cycling of NaFeO2 vs. Na metal using a specifically designed in situ cell,[4] and analysed using an alternative and innovating data analysis approach based on chemometric tools such as Principal Component Analysis (PCA) and multivariate curve resolution (MCR).[5,6] This approach, which allows the unbiased extraction of all possible information from the operando data, enabled the stepwise reconstruction of the “real” spectral components occurring during the cycling of NaFeO2. In this way, a clear description of the electrochemically active iron species could be obtained, allowing a clearer comprehension of the cycling mechanisms of this material vs. sodium. References [1] J. Zhao, L. Zhao, N. Dimov, O. Shigeto, T. Nishida, J. Electrochem. Soc. 160 (2013) A3077. [2] H. Yoshida, N. Yabuuchi, S. Komaba, in:, ECS Meet. Abstr. MA2012-02, ECS, Honolulu, 2011, p. 1850. [3] P. Barpanda, Chem. Mater. 28 (2016) 1006. [4] J.-B. Leriche, S. Hamelet, J. Shu, M. Morcrette, C. Masquelier, G. Ouvrard, M. Zerrouki, P. Soudan, S. Belin, E. Elkaïm, F. Baudelet, J. Electrochem. Soc. 157 (2010) A606. [5] R. Tauler, Chemom. Intell. Lab. 30 (1995) 133. [6] A. Voronov, A. Urakawa, W. van Beek, N.E. Tsakoumis, H. Emerich, M. Rønning, Anal. Chim. Acta 840 (2014) 20. [less ▲]

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See detailApplication d'un bridge dentaire pour traiter de larges fistules orales chez 2 chevaux
Storms, Nazaré ULiege; de la Rebière de Pouyade, Geoffroy ULiege; Grulke, Sigrid ULiege et al

Poster (2018, November 08)

Introduction : Chez les chevaux, des fistules oro-sinusales et oro-cutanées peuvent survenir après expulsion dentaire. Souvent, celles-ci se résolvent après débridement de la fistule et application d’un ... [more ▼]

Introduction : Chez les chevaux, des fistules oro-sinusales et oro-cutanées peuvent survenir après expulsion dentaire. Souvent, celles-ci se résolvent après débridement de la fistule et application d’un ‘bouchon’ temporaire au niveau de l’alvéole de la dent expulsée. Lorsque la fistule est très large, la stabilisation de ce bouchon est parfois impossible et des techniques de comblement de la fistule par transposition musculaire (levator nasolabialis ou levator labii superioris) peuvent aboutir à une étanchéité incomplète. Nous décrivons ici l’application d’un bridge dentaire pour traiter de larges fistules oro-sinusale ou oro-cutanée chroniques, ainsi que nos résultats chez 2 chevaux. Anamnèse – examen clinique – démarche diagnostique : Le premier cas est un hongre Westphalien de 12 ans, présenté pour jetage nasal purulent survenu 4 ans après l’expulsion de la dent 209. Le deuxième cas est un hongre Oldenburg de 5 ans, présenté pour une fistule purulente rostrale à la crête faciale, 7 semaines post expulsion de la dent 208. Des fistules oro-sinusale (cas n°1) et oro-cutanée (cas n°2) ont été mises en évidence par buccoscopie et radiographie. Traitement : Sous anesthésie générale, après débridement de la fistule, un fil de cerclage formant un ‘8’ en prenant appui au niveau des espaces interdentaires rostral et caudal à la fistule a été appliqué au moyen d’une approche orale et transbuccale. Les fils de cerclage se croisant au niveau de la fistule ont ensuite été englobés dans du polyméthylmétacrylate (PMMA) de manière à étanchéifier la cavité buccale tout en évitant le comblement en profondeur de la fistule. Les 2 chevaux ont très bien toléré leur bridge, et leurs symptômes ont rapidement disparu après l’intervention. Le bridge était toujours en place après 2 ans chez le cas 1. Chez le cas 2, le bridge a été retiré après 1,5 an suite au comblement total de la fistule. Discussion : Cette technique présente les avantages de ne pas nécessiter de matériel spécialisé ni coûteux, d’être peu invasive et très bien tolérée par les chevaux. Néanmoins, ce bridge nécessite un ancrage au niveau des espaces interdentaires rostral et caudal à la fistule. Dès lors, cette technique est particulièrement indiquée pour les fistules des alvéoles ‘08’ ou ‘09’ mais moins pour celles des autres dents. Conclusion : L’application d’un bridge composé de PMMA et de fil de cerclage est une technique peu invasive et efficace pour traiter certaines larges fistules oro-sinusales et oro-cutanées. [less ▲]

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See detailScientific literature uses in Geography: indexing and “overlap” in four bibliographic tools
Stirbu, Simona ULiege; Greco, Ninfa ULiege

Poster (2018, November 06)

This research aims to evaluate the interest for geographers to use Google Scholar(GS) over the commercial databases Web of Science (WoS), Scopus (multidisciplinary databases), and GeoRef (specialized in ... [more ▼]

This research aims to evaluate the interest for geographers to use Google Scholar(GS) over the commercial databases Web of Science (WoS), Scopus (multidisciplinary databases), and GeoRef (specialized in geosciences). To achieve our main objective, we performed searches in order to verify to which extent researchers' citations are covered by the search engine and the three commercial bibliographic databases mentioned. Our sample consists of the citations of three dissertations in Geography presented in the Uliège (Belgium) Department of Geography during the 2014-2015 academic year, respectively in Climatology, Tourism and Geomatics. In order to have an overview of the results’ “overlap” for the bibliographic tools, and their "unique" contribution, Venn diagrams were constructed for each thesis. The Venn diagrams indicate that GS finds almost all bibliographic references and returns the greatest number of "unique" results. The “overlap” rates between the commercial databases is very high and GeoRef stands out with very low results. GS is an important and rich complementary tool, with a more varied panel of publishers, languages and countries of publication. [less ▲]

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See detailDoes in vitro protocol predict the nutritional value of thermally treated cereals?
Huart, François ULiege; Bera, François ULiege; Blecker, Christophe ULiege et al

Poster (2018, November 06)

Several in vitro protocols were proposed to rapidly evaluate the nutritional value of cereal and food ingredients. These In vitro protocols are based on restricted parameters and may involve results ... [more ▼]

Several in vitro protocols were proposed to rapidly evaluate the nutritional value of cereal and food ingredients. These In vitro protocols are based on restricted parameters and may involve results differing to the reality once assessed in vivo. Moreover, information about some in vitro parameters is sometimes insufficiently described. In the present study, a three steps in vitro digestion simulation developed by Tervila-Wilo et al. (1996) to mimic digestion in poultry digestive tract was assessed and compared with in vivo digestibility of corn grain harvest at two moisture contents and dried at three temperatures. In vitro digestion simulation showed that increasing drying temperature led to an increase in in vitro digestibility of dry matter (IVDMD) while in vivo digestibility of dry matter (DM) measured on force fed broilers chickens decreased in the same conditions. To improve this in vitro digestion model, the effect of amylase activity of pancreatin, substrate concentration, corn flour particles reduction and agitation were investigated. Results showed that increasing amylase activity of pancreatin and decreasing substrate concentration improved DM digestibility through starch digestibility while corn flour particles reduction had little effect on its final DM digestibility. Rotational agitation greatly improved starch digestibility and therefore DM digestibility, even at low speed. This increase in DM digestibility reached 20% and was attributed to the better contact between substrate and enzymes and a reduction in solution viscosity under constant agitation. A good adjustment of these parameters would improve the ability of in vitro digestion simulation to accurately predict nutritional value of thermally treated cereals. They have to be taken into account on the implementation of in vitro digestion simulation aiming to predict in vivo performances of consumers. [less ▲]

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See detailHow Much Do We Gain From Greater Personalisation?
Uyttendaele, Vincent ULiege; Knopp, Jennifer L.; Shaw, Geoffrey M. et al

Poster (2018, November)

Objective: STAR (Stochastic TARgeted) is risk-based glycaemic control (GC) using prediction of future insulin sensitivity (SI) variability to safely dose insulin and nutrition, where SI variability is the ... [more ▼]

Objective: STAR (Stochastic TARgeted) is risk-based glycaemic control (GC) using prediction of future insulin sensitivity (SI) variability to safely dose insulin and nutrition, where SI variability is the key driver in GC difficulty and hypoglycaemia. Currently, STAR uses a 2D stochastic model where current identified patient-specific SI is used to predict future SI variability in a cohort-specific sense. This study assesses the impact on GC performance of a new, more patient-specific 3D stochastic model, using previous and current SI values to predict metabolic variability. Method: Bi-variate and tri-variate Gaussian kernel density methods are used to estimate conditional probability estimation of future SI knowing current SI (2D model) and also previous SI (3D model). Models are built randomly using 411 (70%) of retrospective GC episodes. They are tested using clinically validated virtual trials on the 176 (30%) remaining patients, repeating 3 times (N=528 episodes). Safety, performance, and workload are compared. Results: Out of the total 528 simulated episodes, workload was similar (11.6 measures/day). Performance was similar (90% in 80-145mg/dL band), but tighter for the 3D model (78% vs 74% in 80-125mg/dL band). Median BG level was lower for the 3D model (108 [99, 120] vs. 113 [103, 124]mg/dL), with higher insulin (3.0 [1.5, 5.0] vs 2.5 [1.5, 4.0] U/h) and nutrition (99 [66, 100] vs 92 [70, 100] % goal feed). Safety was very slightly better for the 2D model (2% vs 3% BG<72mg/dL; 1% vs 1.4% BG<40mg/dL). Conclusions: The new, more personalised 3D stochastic model provides moderately improved performance and similar safety and workload. Overall, results suggest greater personalization in predicting variability can improve STAR GC performance and justify implementation to see if it improves outcomes. [less ▲]

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See detailAqueous N-Doped TiO2 Catalysts for Visible Light Photocatalytic Applications
Mahy, Julien ULiege; Cerfontaine, Vincent; Poelman, Dirk et al

Poster (2018, November)

In this work, TiO2 prepared with an aqueous sol-gel synthesis by peptization process is doped with nitrogen precursor to extend its activity towards the visible region. Three N-precursors are used: urea ... [more ▼]

In this work, TiO2 prepared with an aqueous sol-gel synthesis by peptization process is doped with nitrogen precursor to extend its activity towards the visible region. Three N-precursors are used: urea, ethylenediamine and triethylamine. Different molar N/Ti ratios are tested and the synthesis is adapted for each dopant. For urea- and trimethylamine-doped samples, anatase-brookite TiO2 nanoparticles of 6–8 nm are formed, with a specific surface area between 200 and 275 m2 g−1. In ethylenediamine-doped samples, the formation of rutile phase is observed, and TiO2 nanoparticles of 6–8 nm with a specific surface area between 185 and 240 m2 g−1 are obtained. X-ray photoelectron spectroscopy (XPS) and diffuse reflectance measurements show the incorporation of nitrogen in TiO2 materials through Ti–O–N bonds allowing light absorption in the visible region. Photocatalytic tests on the remediation of water polluted with p-nitrophenol show a marked improvement for all doped catalysts under visible light. The optimum doping, taking into account cost, activity and ease of synthesis, is up-scaled to a volume of 5 L and compared to commercial Degussa P25 material. This up-scaled sample shows similar properties compared to the lab-scale sample, i.e., a photoactivity 4 times higher than commercial P25. [less ▲]

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See detailAntiplasmodial activity and interaction between cannabidiol and artemisinin
Coelho Cristino Mamede; Degotte, Gilles ULiege; Ledoux, Allison ULiege et al

Poster (2018, November)

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See detailOptimization of calcium phosphate ceramic
Tilkin, Rémi ULiege; Regibeau, Nicolas ULiege; Grandfils, Christian ULiege et al

Poster (2018, October 29)

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently ... [more ▼]

During the past few years, tissue engineering has become one of the most promising techniques to maintain, improve, or reconstruct human tissue, even complete human organs. This solution is frequently based on the realization of temporary porous matrices, also called "scaffolds". These materials are highly porous matrices designed to structure the development of cells, but also to guarantee the function of the implant during the regeneration process. Several materials have been proposed for the conception of scaffolds, including calcium phosphate ceramics. Among these materials, the bioceramic class is composed of hydroxyapatite (HA), Ca5(PO4)3(OH), and β-tricalcium phosphate (TCP), β-Ca3(PO4)2. These two products are frequently used, because of their chemical and structural similarity to human bones. These similarities explain good scores observed in vitro and in vivo in terms of biocompatibility and cell colonization. [less ▲]

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See detailProduction of pure and functional Bone Morphognetic Protein-2
Robert, Charly ULiege; Matagne, André ULiege; Filée, Patrice et al

Poster (2018, October 27)

Bone Morphognetic Protein-2 (BMP-2), which belongs to the transforming growth factor β (TGF-β) superfamily, plays an important role during bone regeneration and repair, and also during various stages of ... [more ▼]

Bone Morphognetic Protein-2 (BMP-2), which belongs to the transforming growth factor β (TGF-β) superfamily, plays an important role during bone regeneration and repair, and also during various stages of embryonic development. Noggin is a BMP-specific antagonist implicated in the regulation of BMP signaling pathways. Both BMP-2 and Noggin are dimeric molecules with each subunit containing several intramolecular disulfide bonds. This complex disulfide bonds network combined with the presence of large hydrophobic patches on the surface of these proteins cause their low solubility in aqueous solutions. As a result, BMP-2 and Noggin are commonly produced as inclusion bodies in Escherichia coli. The recovery of soluble and active molecules through in vitro refolding is difficult and the overall yield is low. Here we propose a new and simple method to produce biologically active BMP-2 and Noggin. Refolding of BMP-2 and Noggin was achieved in a one-step dilution and hydrophobic or ion exchange chromatography were used to purify the refolded proteins. The best conditions yielded significant quantities of dimeric BMP-2 and Noggin, with purity above 95%. Both BMP-2 and Noggin were characterized by optical spectroscopies and its biological activity was tested by measuring alkaline phosphatase activity in ATDC5 cells. These experiments indicated that our BMP-2 and Noggin display structural and functional properties similar to their commercial counterparts. [less ▲]

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See detailEffectiveness and cost-benefit study to encourage herd owners in sharing costs for vaccination programme against bluetongue serotype-8 in Belgium
Cargnel, Mickaël ULiege; Van der Stede, Yves; Haegeman, Andy et al

Poster (2018, October 26)

This study evaluated the effectiveness of vaccination against BTV-8 in Belgium and has shown that the interaction between the time since the first injection and the second injection of the primo ... [more ▼]

This study evaluated the effectiveness of vaccination against BTV-8 in Belgium and has shown that the interaction between the time since the first injection and the second injection of the primo-vaccination is significantly associated to the change in serology showing vaccine efficiency induces antibodies production. This study also clearly confirms the benefit of vaccination by reducing economic impact of treatment and production losses, especially in dairy cattle herds. [less ▲]

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See detailPyocolpos in a spayed queen with imperforate hymen: a case report
Egyptien, Sophie ULiege; Shimizu, Naomi ULiege; Anne-Archard, Nicolas ULiege et al

Poster (2018, October 26)

A 1-y-old sterilized queen was presented for dysuria. Abdominal palpation elicited pain and revealed a firm, well-circumscribed mass dorsal to the bladder. Ultrasonography confirmed a caudal fluid filled ... [more ▼]

A 1-y-old sterilized queen was presented for dysuria. Abdominal palpation elicited pain and revealed a firm, well-circumscribed mass dorsal to the bladder. Ultrasonography confirmed a caudal fluid filled abdominal structure extending into the pelvic cavity, displacing the colon dorsally and the urethra ventrally. Retrograde vagino-urethrography showed contrast in the vestibule, urethra and urinary bladder. Imperforate membrane at the vestibulo-vaginal junction with secondary vaginal distension was highly suspected. During surgery, a distended by purulent content vagina was observed, sub-total vaginectomy was performed. Bacterial culture showed Enterobacter cloacae. Definitive diagnosis of imperforate hymen is usually achieved by vaginoscopy. Retrograde vagino-urethrography can be used in some cases. This is the first report of imperforate hymen in the queen and also is the first case of pyocolpos. Origin of infection remains unclear. Contamination during neutering or via partial perforation of the hymen may be suspected. Enterobacter cloacae is a Gram - opportunistic pathogen of the urogenital tract of humans and animals. It is involved in multidrug-resistance spreading but its prevalence and clinical impact in veterinary medicine is unknown. In conclusion, this first report of persistent hymen in the queen highlights vagino-urethrography usefulness for diagnosing imperforate hymen in small patients, as well as the inclusion of congenital abnormality in the differential diagnosis of dysuria and the feasibility of sub-total vaginectomy by abdominal approach. Finally, it raises the question of Enterobacter cloacae’s implication in nosocomial infection in veterinary medicine. [less ▲]

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See detailLong-term outcome of the transobturator vaginal tape inside out (TVT-O) for the treatment of urethral sphincter mechanism incompetence in female dogs
Hamon, Martin ULiege; Hamaide, Annick ULiege; Noël, Stéphanie ULiege et al

Poster (2018, October 26)

Urethral sphincter mechanism incompetence (USMI) is the most common cause of acquired urinary incontinence in female dogs. Surgery is recommended if the animal does not respond to medical treatment or ... [more ▼]

Urethral sphincter mechanism incompetence (USMI) is the most common cause of acquired urinary incontinence in female dogs. Surgery is recommended if the animal does not respond to medical treatment or becomes refractory, if side effects develop or if owners are reluctant to administer long-term medication. Stress urinary incontinence in women is mainly treated surgically by vaginal placement of suburethral slings, including the transobturator vaginal tape inside out. This technique was reported in female dogs in 2010 and short-term results were promising. The objective of the present study was to evaluate the long-term outcome of the TVT-O in female dogs. TVT-O tape was inserted in 12 incontinent bitches diagnosed with USMI. Follow-up information was evaluated by a telephone questionnaire and a continence score was attributed. One year after surgery, 7 of 12 dogs were completely continent (58%). Two dogs were removed from the long-term analysis (1 dead and 1 lost). At a median follow-up time of 85 months (range 28 to 95 months), 4 /10 dogs were completely continent without medical treatment. Incontinence recurred in 6 dogs at a median time of 2 months after surgery (range 1 to 20 months). Among those, 4/6 regained continence and 2/6 had sporadic episodes of incontinence, with additional medical treatment. No postoperative complications were encountered. TVT-O alone was successful in maintaining long term continence in 40% of the dogs. Additional postoperative medical treatment was effective in restoring continence in another 40 % of dogs. TVT-O provides an alternative treatment of USMI in female dogs, that is safe and less invasive than standard surgical techniques. [less ▲]

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See detailParotid purulent sialocoele treated by grass seeds extirpation
Picavet, Pierre ULiege; Grauwels, Magda ULiege; Etienne, Anne-Laure ULiege et al

Poster (2018, October 26)

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See detailIdentification of unconventional O serotypes of enteropathogenic (EPEC) Escherichia coli from intestinal contents of healthy cattle at slaughterhouse
Ikeda, Rie; Habets, Audrey ULiege; Duprez, Jean-Noël ULiege et al

Poster (2018, October 26)

Escherichia coli producing the attaching-effacing (AE) lesion are called enteropathogenic E. coli (EPEC) and are responsible for diarrhoea in animals and humans. EPEC are subdivided into typical (t) EPEC ... [more ▼]

Escherichia coli producing the attaching-effacing (AE) lesion are called enteropathogenic E. coli (EPEC) and are responsible for diarrhoea in animals and humans. EPEC are subdivided into typical (t) EPEC producing the “Bundle Forming Pili” type 4 fimbriae and isolated from humans, and atypical (a) EPEC not producing the BFP and isolated from humans and animals, including diarrheic young calves and healthy adult cattle. tEPEC and several aEPEC belong to specific serotypes, but different aEPEC can also belong to classical Shigatoxigenic E. coli (STEC) O serotypes (O26, O103, O111, O121, O145, O157, O165) and derive from STEC after loss of genes encoding the Shiga toxins. In 2014 several hundreds of EPEC from healthy cattle at two slaughterhouses in Wallonia, but the prevalence of the classical STEC O serotypes was low and all were negative for the recently described O80 EPEC serotype. The aim of this study was to identify 6 other unconventional O serotypes (O123/186, O146, O156, O177, O182, O183) recently identified by PCR in different bovine aEPEC and STEC by one of us, amongst 312 EPEC isolated at slaughterhouse that previously tested negative, applying two triplex PCRs either for the O146_O182_O183 or for the O123/186_O156_O177 serotypes. So far the first triplex PCR identified 1 O146-positive and 8 O182-positive EPEC. The second triplex PCR identified 30 O156. The further steps of this study are: (i) the identification of still other unconventional serogroups among EPEC (ii) the identification of their H antigen-encoding genes; (iii) the comparison of these bovine EPEC between themselves, with EPEC from diarrheic calves and with STEC from cattle and humans belonging to the same serotypes. [less ▲]

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See detailSO2 effect on oxidative degradation of monoethanolamine for post combustion CO2 capture
Benkoussas, Hana ULiege; Dubrulle, Louis ULiege; Léonard, Grégoire ULiege

Poster (2018, October 23)

The most conventional amine used in the post combustion capture technology, based on chemical absorption, is monoethanolamine (MEA). Depending on the composition of the flue gas to be treated, several ... [more ▼]

The most conventional amine used in the post combustion capture technology, based on chemical absorption, is monoethanolamine (MEA). Depending on the composition of the flue gas to be treated, several mechanisms of amine degradation occur. This leads to a loss of CO2 absorption capacity and may give rise to products that are environmentally difficult to dispose of. Results from CO2 capture pilot plants showed that oxidative degradation due to the presence of oxygen in the flue gas is the main degradation pathway. Furthermore, MEA degrades when exposed to a flue gas containing an amount of sulfur dioxide (SO2). Until now there is not a clear statement about the effect of SO2 on oxidative degradation of MEA. The goal of this study is to identify the impact of the presence of SO2 on the oxidative degradation of MEA. [less ▲]

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See detailMultilingual resource to disseminate main concepts in Family Medicine and Primary care
Jamoulle, Marc ULiege; Bae, Jong-Myon; Pizzanelli, Miguel et al

Poster (2018, October 19)

Background: General practice/ Family Medicine (GP/FM) and Primary Health Care (PHC) Knowledge Management (KM) - High complexity by intertwined human and technical approaches - Content not harmonized. No ... [more ▼]

Background: General practice/ Family Medicine (GP/FM) and Primary Health Care (PHC) Knowledge Management (KM) - High complexity by intertwined human and technical approaches - Content not harmonized. No uniform table of content for both disciplines - Classification system available only for clinical purposes (ICPC 1987- ongoing) - No existing contextual features classification Methods - Qualitative analysis of 2300 abstracts of GP congresses - Elaboration of a taxonomy called Q-Codes about contextual information (ie not clinical), - Complementary to the International Classification of Primary care (ICPC-2) - Using online HeTOP semantic base (OWL-2) to develop terminological records - Linking concept to existing terminologies and knowledge repositories (PubMed- DBpedia-Babelnet) - Multilingual translation by dedicated GPs worldwide (10 languages – more ongoing) - Experimental use of Q-Codes in various KM situations Results • GP/FM & PHC evolutionary knowledge base on www.hetop.eu/Q (exportable in Excel and OWL-2) • 182 Q-Codes Uniform Resource Identifier (URI) in 10 languages: ex : Korean Shared Decision Making in Korean • Experiments are ongoing and presented on http://3cgp.docpatient.net/(abstracts indexing, pedagogy, master thesis indexing, discussion indexing, automatic annotators, e-learning application) Conclusion Indexing of the GP/FM literature (congress abstracts, master's and doctoral thesis, group discussions) enhance the accessibility of research results and promote the emergence of networks of researchers. The work is ongoing and will be maintained by the Q-Code working group into the WONCA International Classification Committee. More on : Jamoulle & all EJGP Dec 2017. [less ▲]

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See detailHow does cognitive fatigue affect Young, Middle-aged, and Older people? A distribution analysis of Time-on-Task effect by fitting the ex-Gaussian parameters to the response time distributions.
Gilsoul, Jessica ULiege; Collette, Fabienne ULiege

Poster (2018, October 19)

Introduction. Young people classically experiment cognitive fatigue after long-lasting cognitive tasks (Time-on-Task effect), as evidenced by changes in response time (RT) distribution (Wang et al., 2014 ... [more ▼]

Introduction. Young people classically experiment cognitive fatigue after long-lasting cognitive tasks (Time-on-Task effect), as evidenced by changes in response time (RT) distribution (Wang et al., 2014). As RT distributions are often positively skewed, the ex-Gaussian distribution (the convolution of the exponential and the Gaussian distributions) fits RT data relatively well. The ex-Gaussian distribution comprises three parameters: mu & sigma are mean & SD of the Gaussian component while tau describes the exponential component (the increase in extreme RT). In Young people, mu and sigma are not fatigue sensitive while tau is well known to suffer from Time-on-Task. Aim. To replicate the results previously found in Young people (Wang et al., 2014) and to test this Time-on-Task effect in Middle-aged and Older people by fitting the three ex-Gaussian parameters to RT distributions. Method. Procedure. Twenty-one Young (8 males; MAge=22.43), 17 Middle-aged (7 males; MAge= 50.47), and 17 Older (9males; MAge=65.06) were recruited. Based on Wang et al. (2014) and Wang et al. (2016), we administered a modified version of a computerized Stroop task (Stroop, 1935) during 160 min. In our task, different words (“BLUE”, “RED”, “YELLOW”, “GREEN”) or the symbol “XXXX” appeared one at a time printed in one of the following colors: blue, red, yellow, or green. The task was composed of facilitatory (FA) items (“BLUE” written in blue), interfering (I) items (“BLUE” written in red), and neutral (NE) items (“XXXX” symbol printed in color), these latter ones systematically appearing one item out of two. Since RT data are rarely normally distributed but are often positively skewed, classical parameters like mean and SD are not good candidates to properly describe the data (Heathcote et al., 1991). Therefore, in a very similar way to Wang et al. (2014) in a Young group, we decided to fit the ex-Gaussian distribution to our RT data in order to fully capture the Time-on-Task effect on RT distribution in our Young, Middle-aged, and Older groups. Among the available techniques, the SIMPLEX algorithm is well used for fitting the ex-Gaussian distribution (Lacouture & Cousineau, 2008). However, in certain cases, the parameter search with this method may fail to converge. To obtain a very efficient algorithm, we decided to base our search procedure both on the algorithm of Nelder & Mead (1965) which is gradient-free but also on the Greedy algorithm technique allowing to be flexible in the starting values it takes. Statistical analyses. The duration of the task was divided into four blocks of 40min each and we fitted the ex-Gaussian parameters (mu, sigma, tau) to the RT distributions in those four blocks, separately for each item types (FA, I, NE). Analyses were performed with the GLIMMIX procedure of SAS on mu and tau but not on sigma because of homoscedasticity violation. Each of the 6 remaining measures (FA_mu, I_mu, NE_mu, FA_tau, I_tau, NE_tau) was then analyzed with the GLIMMIX repeated measure and multiple comparisons post-hoc of Tukey. Each parameter was explained by Group, Block (the repeated variable), Group X Block interaction, Sex, Education, and Depression status. Results Mu parameter. All three FA_mu, I_mu, and NE_mu measures showed a significant effect of Group (all p < .01). Tukey post-hoc further showed significant differences between the Young group and both the Middle-aged and the Older groups, with the Young group having a smaller value of mu than the two other groups for the three measures. Moreover, the Group X Block interaction showed a tendency (p = .077) of significance on the I_mu measure. Tukey post-hoc on this interaction showed that the Older group had a greater mu in Block 4 as compared to Block 1 and Block 2 while the Young and the Middle-aged groups did not show any difference. Finally, none of the demographical variables was significant on mu parameter. Tau parameter. FA_tau showed a significant effect of Block (p = .002). Tukey post-hoc showed an almost significant difference between the 1st and the 4th Block (p = 0.05) and a significant difference between the 2nd and the 4th Block (p<.001). Exploratory Tukey post-hoc on the Group X Block interaction showed that Middle-aged had a significant difference between the 2nd and the 4th Block (p = .002) but also between the 3rd and the 4th Block (p = .015) while the Young and the Older groups did not show any difference. None of the demographical variables was significant on FA_tau. I_tau showed a tendency of significance on the Group X Block interaction (p = .074). Tukey post-hoc showed that Middle-aged had a significant difference between the 1st and the 4th (p = .041) but also between the 2nd and the 4th Block (p = .03). I_tau also showed a significant effect of Sex (p = .048), with the male status being associated with greater values of I_tau as compared to the woman status. NE_tau showed a significant effect of Block (p<.0001). Tukey post-hoc showed significant increase in tau between the 1st and the 3rd Block, the 1st and the 4th Block, but also between the 2nd and the 4th Block (all p < .001). Exploratory Tukey post-hoc on the Group X Block interaction showed that Young people had an increase in tau between the 1st and the 3rd Block (p = .007) but also between the 1st and the 4th Block (p = .001). Moreover, Middle-aged people had an increase in tau between the 1st and the 4th Block (p = .008) but also between the 2nd and the 3rd (p = .015) and between the 2nd and the 4th Block (p<.001). Finally, the Older group had a significant increase between the 1st and the 4th Block (p = .02). None of the demographical variables was significant on NE_tau. Discussion We demonstrated for the first time that mu parameter undergoes a Group effect. Indeed, all three measures (FA_mu, I_mu, and NE_mu) showed a significant effect of Group, with Young people showing less slowdown than Middle-aged and Older. It is consistent with literature showing the classic age effect on the slowdown of reaction time (Salthouse, 2000). Furthermore, mu did not show any Block effect, which is in agreement with the “ex-Gaussian” literature (Wang et al., 2014). As compared to the classic mean, mu is free from extreme values given that they are comprised in the tau parameter, what can explain the absence of Time-on-Task effect on the mu parameter. As proposed by Hohle (1965), mu would represent the peripheral/motor processes while tau would represent the decisional component of RT data. Therefore, if mu tends to represent procedural processes, our study is also in great agreement with that of Borragán et al. (2016) showing an improvement in procedural learning – in our case, an absence of impairment in procedural learning - in situation of cognitive fatigue. Only the Older group showed difference between the two first and the last Block on I_mu, probably because they are more prone to suffer from interference and decrements in inhibition (Hasher & Zacks, 1988) as compared to the two other groups, leading them to show fatigue installation specifically on the Interfering items. By contrast, FA_tau and NE_tau suffered well from Time-on-Task effect, which is also in agreement with the ex-Gaussian literature showing increases in tau with the time spent on a task (Wang et al., 2014). Exploratory Tukey post-hoc analyses showed that the Middle-aged group had significant increases in tau with Time-on-Task on the three tau measures (FA_tau, I_tau, and NE_tau) while the two other groups only got significant difference with Time-on-Task for NE_tau. We made the hypothesis that the three groups showed significant differences in the NE_tau measure because NE items were the most predictable. Indeed, as they were appearing one out of two in our task, it possible that their greater predictability have led participants to a lack of motivation and then a lack of top-down attention necessary to counteract task-disengagement for NE items, making the motivation drop and boredom good candidates to explain cognitive fatigue effects (Boksem & Tops, 2008). Moreover, we did not observe a classic age affect in the sense that Middle-aged seemed to suffer more than Older from Time-on-Task. We claim it is possible that Middle-aged people be the more fatigued group. Indeed, it is a population fully busy, immersed in active life but also having generally great responsibilities, leading them to be more susceptible to cognitive fatigue and to show fatigue signs on the three measures as compared to the other groups. In conclusion, the combined use of mu and tau parameters to characterize RT distributions of Young, Middle-aged, and Older has allowed to more accurately understanding fatigue effect in those populations. First, we demonstrated that the three groups were not impaired in their motor/peripheral processes with Time-on-task except for the Older on the Interfering items, what is not surprising given their baseline difficulties in inhibition (Hasher & Zacks, 1988). Secondly, the three groups were disabled in their decisional processes as indexed by increases in their extreme RT, Middle-aged being the most cognitively fatigued. [less ▲]

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See detailMolecular mechanisms of gap junction assembly in the cochlea
Defourny, Jean ULiege; Thelen, Nicolas ULiege; Thiry, Marc ULiege

Poster (2018, October 19)

Hereditary hearing loss affects about 1 per 1000 children. Mutations in GJB2 and GJB6, which encode connexins 26 and 30 (Cx26 and Cx30) involved in cochlear homeostasis, cause autosomal syndromic and non ... [more ▼]

Hereditary hearing loss affects about 1 per 1000 children. Mutations in GJB2 and GJB6, which encode connexins 26 and 30 (Cx26 and Cx30) involved in cochlear homeostasis, cause autosomal syndromic and non-syndromic hearing loss. In cochlear non-sensory supporting cells, Cx26 and Cx30 form two types of homomeric and heteromeric gap junctions. Deciphering the assembly mechanisms of these channels in situ should represent an advance in understanding the pathogenic significance of many of these mutations. Connexin trafficking and delivery to the gap junction plaque usually occurs from the peripheral non-junctional membrane region, called perinexus, and requires the actin network. Combining immunolabelling and organotypic in vitro assay, we found that Cx30 homomeric and Cx26/Cx30 heteromeric channels exhibit distinct assembly mechanisms in the cochlea. When expressed as homomeric channels, Cx30 preferentially associates with the actin network in the perinexus and strongly relies on it for gap junction plaque assembly. In contrast, we found that Cx26/Cx30 heteromeric gap junction plaques are devoid of perinexus and associated actin network, and resist to actin-depolymerizating drug. This supports that Cx26/Cx30 oligomers could be directly delivered from the interior of the cell to the junctional plaque. Altogether, our data provide a novel insight in homomeric and heteromeric gap junction plaque assembly in the cochlea. [less ▲]

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See detailArousals during sleep are associated with brain tau burden in healthy older individuals
Chylinski, Daphné ULiege; Rudzik, Franziska; Coppieters't Wallant, Dorothe ULiege et al

Poster (2018, October 19)

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See detailNear-death experiences Are they self-defining?
Cassol, Helena ULiege; D'Argembeau, Arnaud ULiege; Charland-Verville, Vanessa ULiege et al

Poster (2018, October 19)

Background: Near-Death Experiences (NDEs) are profound psychological events with highly emotional and self-related content, typically encompassing transcendental and mystical elements and occurring when ... [more ▼]

Background: Near-Death Experiences (NDEs) are profound psychological events with highly emotional and self-related content, typically encompassing transcendental and mystical elements and occurring when people come close to death (Greyson, 2000). These experiences appear to have significant consequences on peoples’ lives (so-called "NDE experiencers" or "NDErs"; e.g., Noyes, 1980). Given their documented life-transforming effects and their reported importance, NDE memories appear to share similarities with a particular type of autobiographical memories referred to as a self-defining memories (SDMs; Blagov and Singer, 2004). SDMs are the building blocks of identity (Blagov and Singer, 2004) and contribute, in particular, to the sense of self-continuity (Conway et al., 2004) which represents the ability to consider oneself as an entity that extends back into the past and forward into the future (Chandler, 1994). Objectives: This study aimed at 1) assessing if NDE memories are considered as SDMs and 2) determining whether the potential self-defining dimension of NDEs is due to their phenomenal content or their circumstances of appearance (i.e., presence or absence of impeding death). Methods: 71 participants were screened using the Greyson NDE scale (48 real NDErs and 23 NDErs-like who had lived a similar experience in absence of life threat; Greyson, 1983). This 16-item multiple-choice validated scale enables to quantify the richness of the experience (scores ranging from 0 to 32) and allows a standardized identification of NDEs (cut-off score of 7). Participants described their two main self-defining memories (SDMs) and completed the Centrality of Event Scale (CES; Berntsen and Rubin, 2006) for each one of them. The CES is a 20-item scale (scores ranging from 0 to 100) designed to assess how central the event is to their identity. Proportions of NDErs who recalled their NDE were calculated for each subgroup (real NDErs and NDErs-like) and a Pearson’s chi square test was performed to compare ratios between them. Later, all participants were divided into two subgroups depending on whether or not they recalled their NDE (no matter its context of occurrence; "NDE recalled" and "NDE not recalled"). The last step of analyses focused on the CES scale and was only carried out on the “NDE recalled” subgroup. Differences in CES total scores between the NDE memory and the other SDM were assessed using a Student’s t-test. Additionally, a Spearman’s correlation was performed to examine associative strength between CES and Greyson NDE scale total scores. Results: Real NDErs and NDErs-like did not differ regarding the proportion of NDErs who recalled their NDE (30 real NDErs out of 48 and 11 NDErs-like out of 23; p=0.24), suggesting that the self-defining aspect of the experience could be explained by its phenomenological content rather than context of occurrence. These participants (n=41) rated the NDE memory as more central to their identity as compared to the other SDM (p<0.001). Furthermore, the richness of the NDE memory (Greyson NDE scale scores) was positively associated to its centrality (CES scores; p<0.01). Conclusions: The self-defining status of NDE memories confirms that they constitute an important part of NDErs’ personal identity and highlights the importance for clinicians to facilitate their integration within the self. SDMs are indeed essential to one’s sense of self-continuity, which is crucial for psychological well-being. [less ▲]

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See detailCrystal chemistry and polymorphism of an iron-tungsten mixed oxide
Caubergh, Stéphane ULiege; Jungers, Thomas ULiege; Mahmoud, Abdelfattah ULiege et al

Poster (2018, October 17)

Among the complex metal oxides, the crystal chemistry of the iron-tungsten oxide Fe2WO6 is of interest because of the existence of several crystal structures with different organizations of the FeO6 and ... [more ▼]

Among the complex metal oxides, the crystal chemistry of the iron-tungsten oxide Fe2WO6 is of interest because of the existence of several crystal structures with different organizations of the FeO6 and WO6 octahedra. The conditions of polymorphism are still unclear: structures were reported in the literature for only two of the three polymorphs (α and γ) (image A) and very little is known about the third polymorph (β). In this work, our aim is to synthesize the different polymorphs of Fe2WO6 as pure products in order to be able to investigate the crystal structures and properties in more detail. We report about the synthesis procedures that we have developed starting from aqueous solutions to minimize the effects of diffusion length and maintain a homogeneous distribution of the cations during the phase formation. The stable solutions were spray-dried in order to obtain spherical particles of dried powder as small and homogeneous as possible (around 1 µm). With this technique, we were also able to control and tune the Fe/W ratio and investigate the possibility for a solid solution domain. The dried powders underwent heat treatments at various temperatures and durations before characterization by means of X-Ray Diffraction, Rietveld refinement, Scanning Electron Microscopy and 57Fe Mossbauer spectroscopy. [less ▲]

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See detailMRI Multiparameter measures as relevant explanatory variables of cognitive but not physical fatigue in Multiple Sclerosis
Guillemin, Camille ULiege; LOMMERS, Emilie ULiege; DELRUE, Gaël ULiege et al

Poster (2018, October 13)

Introduction: Fatigue symptoms are highly frequent in patients with multiple sclerosis (MS). However, the underlying brain and clinical mechanisms associated specifically to feeling of cognitive and ... [more ▼]

Introduction: Fatigue symptoms are highly frequent in patients with multiple sclerosis (MS). However, the underlying brain and clinical mechanisms associated specifically to feeling of cognitive and physical fatigue remain largely misunderstood. Aim of study: To investigate whether subjective cognitive and physical fatigue depend on different sets of brain and clinical variables in MS. Methods: Multiparameter mapping (MPM) MRI data (R1 parameter in central grey matter (GM), R2 in cortical and central GM, R2 in cortical white matter (WM), PD in central GM and in cortical WM), and clinical data (age, depression, anxiety, cognitive and physical fatigue scores at the M-FIS; disease duration and EDSS score for patients) were collected among MS patients (n=35) and healthy controls (n=28). Stepwise regression analysis for the cognitive and physical sub-scores of the M-FIS were performed in each group, using clinical and MRI variables. Results: Variance of cognitive M-FIS score for patients was explained with a model taking into account anxiety, depression and measures of cortical and central grey matter (R² = 0.5397, p < .0001). For controls, the model included age, depression and R2 parameter in cortical white matter (R²=0.4137, p = 0.0045). The model with physical M-FIS score included EDSS and anxiety for patients (R² = 0.3834, p = 0.0004), but age and depression for controls (R²= 0.4908, p = 0.0002). Conclusions: These results suggest (1) that the determinants of subjective fatigue vary between MS and control participants; (2) that only cognitive fatigue is linked to brain characteristics. These results stress the need to study cognitive and physical fatigue as two separate entities in MS. [less ▲]

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See detailPerformance-based financing (PBF) in low- and middle-income countries: What is the theory of change, actually?
Paul, Elisabeth ULiege; Bodson, Oriane ULiege; Ridde, Valéry

Poster (2018, October 12)

Performance-based financing (PBF) is expanding in low- and middle-income countries (LMICs) despite mixed results; indeed, it has been criticised for potential perverse effects and unintended effects are ... [more ▼]

Performance-based financing (PBF) is expanding in low- and middle-income countries (LMICs) despite mixed results; indeed, it has been criticised for potential perverse effects and unintended effects are demonstrated. Attributing results to PBF as such is extremely difficult because (i) health systems inherently comprise “structural” incentives and are subject to various reforms beyond PBF; and (ii) there are misunderstandings and controversies about the mere definition and the theory behind PBF. Moreover, PBF schemes encompass different components (e.g. financial premiums conditioned on reaching pre-agreed results, focus and feedback on key performance indicators, coaching, additional resources at facility level, …) and their designs may infinitely vary. Yet, we still did not have a clear and consistent explanation of why and how PBF is supposed to produce results. We explore (i) the theoretical justification of PBF and (ii) the theory of change (ToC) of PBF in the health sector in LMICs. The literature on PBF-related approaches is fragmented across disciplines. -The most commonly used theory to justify PBF is the principal-agent theory. Theory-based evaluation has progressively imposed itself as more appropriate approach to study complex issues. There are few theory-based evaluations and partial attempts to “open the black box” of PBF and identify its ToC / programme theory / causal pathways / mechanisms / transmission of effects. PBF has to a large extent been justified theoretically on the grounds of economic currents – even if the economic rationale is limited by the validity of its (sometimes very) limitative assumptions. For instance, the principal-agent theory does not hold in complex systems such as health. As a conclusion: - The theories used to justify PBF to date are few and insufficiently credible - Studies aimed at uncovering the ToC of PBF are recent and unfinished - Most existing PBF ToCs are not theory-based - Actors need to make the theories underlying their interventions more explicit, disentangling the PBF package - Performance premiums conditioned on reaching a number of predetermined performance criteria may not be justified - If PBF is justified neither by strong theoretical arguments, nor by generalizable evidence, it is definitely marked by a neoliberal ideology, and the promotion of lack of trust and competition over cooperation between actors in the health system - Taboo: Is the debate over PBF definition a way to conceal the debate over PBF ideology [less ▲]

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See detailThe BCCM/ULC collection of cyanobacteria in the spotlight at ULiège
Santoro, Mariano ULiege; Beets, Kim ULiege; Simons, Véronique et al

Poster (2018, October 11)

The ULC public collection of cyanobacteria belongs since 2011 to the consortium of Belgian Co-Ordinated Collections of Microorganisms (BCCM). It aims to conserve ‘ex situ’ a representative portion of the ... [more ▼]

The ULC public collection of cyanobacteria belongs since 2011 to the consortium of Belgian Co-Ordinated Collections of Microorganisms (BCCM). It aims to conserve ‘ex situ’ a representative portion of the biodiversity of cyanobacterial diversity of different origins, with a focus on Antarctic and Artic cyanobacteria isolated from terrestrial and aquatic ecosystems. BCCM/ULC currently holds 174 cyanobacterial strains that are available for researchers who study taxonomy, evolution, biogeography and the adaptation to harsh environmental conditions. The strains are identified by morphology and molecular characterization (based on rRNA sequences) and belong to the Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales orders. This large taxonomic distribution renders it a suitable reference point for phylogenomic and genomic make-up studies. Regular transfer of living cultures ensures the conservation of strains, whose majority are also cryopreserved in order to limit the genetic drift. BCCM/ULC obtained an ISO 9001:2015 certification for public and safe deposits, and for distribution of living strains and genomic DNA. The BCCM policy continuously aims to guarantee a safe fit-for-use microbiological material and data compliant with the rules on access and utilization of the Nagoya Protocol. In addition, BCCM/ULC provides, to clients from academia & industry, a service of morphological identification and molecular characterization, along with other scientific services as tailor-made trainings and collaborations. The public collection is progressively enriched by public deposits from other geographical areas and by incorporating the most interesting strains from the research collection of the host laboratory. The latter is also involved in the valorisation of the collection and collaborations, aiming to study the molecular mechanisms of adaptation to cold stress in polar strains, their production of potential bioactive compounds, to decipher and analyze their genomes and to determine the usefulness of their pigments as ‘traces of life’ in astrobiology. [less ▲]

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See detailStable isotopes reveal trophic plasticity of the bushy scleractinian coral Stylophora pistillata
Sturaro, Nicolas ULiege; Hsieh, Yun Li Eric; Liu, Ling-Wen et al

Poster (2018, October 11)

Most scleractinian corals can derive nutrition either autotrophically or heterotrophically, which allows them to use diverse trophic pathways. Therefore, when facing environmental changes, these organisms ... [more ▼]

Most scleractinian corals can derive nutrition either autotrophically or heterotrophically, which allows them to use diverse trophic pathways. Therefore, when facing environmental changes, these organisms are expected to demonstrate an intrinsic ability to acclimatise through trophic plasticity. Despite the ecological importance of these corals, our understanding of their trophic plasticity is currently impaired by a lack of rigorous research approaches; a failure to consider the intraspecific variability of coral species and an oversimplification of the proxies of heterotrophic habits (e.g. corallite diameter). In order to understand how trophic plasticity could allow them to acclimatise, this study proposed to assess the trophic plasticity of the bushy scleractinian coral Stylophora pistillata. We determined the stable isotope ratios of carbon and nitrogen in the coral host tissues and algal symbionts and compared these in corals inhabiting areas around Taiwan characterised by contrasted temperature (from high to low latitudes) and light levels (from shallow to mesophotic waters). For each area, we evaluated the intraspecific trophic variability by estimating and comparing coral isotopic niches as a proxy for trophic niches. Our results revealed no overlap of the isotopic niches for the host and symbiont from different locations, suggesting that these coral colonies are supported by different core resources. Moreover, the isotopic niche of higher latitude coral colonies was larger than those from the lower latitudes, highlighting a certain trophic plasticity that may be related to more variable environmental conditions in the higher latitudes. Analyses of additional species and locations will provide essential insights into the trophic plasticity of scleractinian corals and how these species might adjust their nutrition in response to environmental changes. [less ▲]

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See detailStromal integrin α11 triggers PDGFRβ signaling to promote breast cancer progression
Primac, Irina ULiege

Poster (2018, October 09)

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See detailPhysico-chemical characteristics of Belgian artisanal cheeses and prevalence of Listeria monocytogenes in these products
Gerard, Amaury ULiege; Daube, Georges ULiege; Sindic, Marianne ULiege

Poster (2018, October 04)

Introduction Regulation (EC) N°2073/2005 demands the absence of L. monocytogenes in 25g of cheeses theoretically allowing the growth of the bacterium. It is assumed that the bacterium cannot develop if ... [more ▼]

Introduction Regulation (EC) N°2073/2005 demands the absence of L. monocytogenes in 25g of cheeses theoretically allowing the growth of the bacterium. It is assumed that the bacterium cannot develop if the pH is under 4.4 or if the water activity (aw) is under 0.92. Furthermore, a combination of pH<5 and aw<0.94 could also prevent its growth (1). A study was performed among Belgian artisanal cheeses in order to investigate their physico-chemical properties, as well as the prevalence of L. monocytogenes. Materials and Methods Sixty-five Belgian artisanal cheeses were collected, respecting geographical dispersion and taking into account several parameters, including type of milk, milk heat treatment, ripening and texture. Salt, fat and dry matter content, as well as aw in the core and pH in the core and on the surface were measured for all samples. The presence of L. monocytogenes in 25g was also tested, with an enumeration in case of detection. For one highly contaminated cheese, the whole batch (10 pieces) was stored until the end of shelf-life. Enumeration was then done with 30 repetitions. Discussion Regarding physico-chemical characteristics, fresh, soft and hard cheeses, pH in the core ranged respectively from 4.5 to 4.7, from 4.7 to 6.9 and from 4.7 to 6.0. On the surface, pH of some cheeses was higher than 7, probably due to the proteolytic activity of the surface microflora. Regarding aw, values were always >0.95. Only based on these pH and aw values, all cheeses from the panel have to be considered as risky regarding L. monocytogenes. L. monocytogenes was isolated from 2 of the 65 cheeses (3.07%).The first one was a semi-hard soft cheese from raw cow milk, for which enumeration was under the LOQ. The second one was a mold-ripened soft cheese from raw goat milk. Enumeration was higher than 4 log cfu/g. This contamination is really high but not really surprising, given that this type of cheese is known for supporting the growth of the pathogen (2). At the end of shelf-life, 14 of 30 samples from this batch were still positive, with levels of contamination ranging from 2 to 6 log cfu/g. A great heterogeneity in the distribution of L. monocytogenes was observed between pieces from this batch, but also inside some pieces. For instance, 3 repetitions in one cheese gave levels of the pathogen ranging from 10 to 17 000 cfu/g. [less ▲]

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See detailDesign of reprocessable poly(ε-caprolactone)-based shape-memory materials by reversible tad chemistry
Riva, Raphaël ULiege; Defize, Thomas; Alexandre, Michaël et al

Poster (2018, October 03)

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See detailDesign of monodisperse polystyrene/polyacrylonitrile core-shell particles used as templating agents and source of carbon
Thangaraj, Vidhyadevi ULiege; Schrijnemakers, Audrey ULiege; Dewalque, Jennifer ULiege et al

Poster (2018, October 03)

In the present work, we synthesized Polystyrene (PS) and Polystyrene/polyacrylonitrile (PS/PAN) core shell particles by surfactant-free emulsion polymerization and characterized them by DLS and TEM. After ... [more ▼]

In the present work, we synthesized Polystyrene (PS) and Polystyrene/polyacrylonitrile (PS/PAN) core shell particles by surfactant-free emulsion polymerization and characterized them by DLS and TEM. After deposition of the PS/PAN particles on a surface, a TiCl4 solution was infiltrated in the interstices before thermal treatment. The carbonization of the PS core and the PAN shell led to voids and carbon in the inorganic layer, respectively. This paved the way to well-organized carbon coated porous TiO2 layers of interest in the field of electronics. [less ▲]

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See detailSynthesis of functional copolymers from cyclic carbonates using controlled radical polymerisation
Scholten, Philip ULiege; Meier, Michael A. R.; Detrembleur, Christophe ULiege

Poster (2018, October 03)

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See detailCESI-MS Workflow for Protein Quantification
Nyssen, Laurent ULiege; Fillet, Marianne ULiege; CAVALIER, Etienne ULiege et al

Poster (2018, October)

Introduction: Sheathless CE-MS interfaces allow increase in sensitivity by coupling low-flow electrospray ionization and tandem MS detection. Peak intensity will depend on spray voltage as well as ... [more ▼]

Introduction: Sheathless CE-MS interfaces allow increase in sensitivity by coupling low-flow electrospray ionization and tandem MS detection. Peak intensity will depend on spray voltage as well as migration and injection conditions. Nevertheless, these parameters influence each other and require methodical optimization to get the most of each instrument. In the present work we share our experience with sheathless CE-MS and neutral coating to analyze peptide and protein samples. Methods: Experiments were conducted on a CESI 8000 capillary electrophoresis holding a neutral coated OptiMS cartridge and coupled to a QT 6500 mass spectrometer. Separation buffer and voltage, curtain gas and source temperature were conserved through experiments. Separation pressure and source voltage were optimized while applying voltage and pressure on separation buffer spiked with the peptide used (pI 9.5 marker from the Advance cIEF starter kit by Sciex). A daily reference run was used to compare modifications to the injection despite variable capillary performance. Finally, shifts in spray voltage due to injection parameters were determined using sequences of runs with different spray voltages. Preliminary results: Decreasing separation pressure from 5 to 1.5 psi increased peptide intensity; electrokinetic injection (EKI) increased peak intensity compared to hydrodynamic injection (HDI); the HDI of a water plug before the EKI increased peak intensity further, as well as a high percentage of acetonitrile in the sample medium. Finally, we compared our initial and our final conditions. In both cases, a positive Q1 scan of 1000 Da/s for m/z 300 to 1000 was acquired, and the electropherograms display the extracted ion current for a 1 m/z interval centered on the m/z of the doubly charged peptide. In the initial method, the peptide was diluted in BGE and was introduced by HDI (1 % of total length); 5 psi pressure were applied to both inlet and outlet; source voltage was 1800 V. When analyzing a 1:160 (v/v) dilution of the peptide, the intensity recorded for [M+2H]2+ was 9.3e7 counts. In the final method, the peptide was diluted in 75:25 acetonitrile:water (v/v) and was introduced by EKI (+ 10 kV 100s). Before the EKI, a HDI of water (0.5 % of total length) was performed, and after the EKI, separation buffer was introduced by HDI (0.5 % of total length). The separation pressure was changed to 1.5 psi and the spray voltage adjusted to 1600 V. When analyzing a 1:160000 (v/v) dilution of the peptide, the recorded intensity of [M+2H]2+ was 9e8 counts. Therefore, following these guidelines, we were able to increase intensity by a 10000 factor. Novel aspect: Frequent monitoring of spray voltage and peak intensity in similar conditions allows good inter-run comparison and troubleshooting. [less ▲]

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See detailMonitoring of hygiene in institutional kitchens in Belgium
Duthoo, E; Krings, Simone ULiege; Daube, Georges ULiege et al

Poster (2018, October)

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See detailA Heartbeat Away From Consciousness: Heart Rate Variability Entropy can discriminate disorders of consciousness and is correlated with resting-state fMRI brain connectivity of the Central Autonomic Network
Riganello, Francesco ULiege; Larroque, Stephen Karl ULiege; Bahri, Mohamed Ali ULiege et al

Poster (2018, October)

Motivation: Heart rate variability (HRV) reflects the heart-brain two-way dynamic interactions[1-5]. HRV entropy analysis quantifies the unpredictability and complexity of the heart rate beats intervals ... [more ▼]

Motivation: Heart rate variability (HRV) reflects the heart-brain two-way dynamic interactions[1-5]. HRV entropy analysis quantifies the unpredictability and complexity of the heart rate beats intervals and over multiple time scales using multiscale entropy (MSE)[6-8]. The complexity index (CI) provides a score of a system’s complexity by aggregating the MSE measures over a range of time scales[8]. Most HRV entropy studies have focused on acute traumatic patients using task-based designs[9]. We here investigate the CI and its discriminative power in chronic patients with unresponsive wakefulness syndrome (UWS) and minimally conscious state (MCS) at rest, and its relation to brain functional connectivity. Methods: We investigated the CI in short (CIs) and long (CIl) time scales in 16 UWS and 17 MCS sedated. CI for MCS and UWS groups were compared using a Mann-Whitney exact test. Spearman’s correlation tests were conducted between the Coma Recovery Scale-revised (CRS-R) and both CI. Discriminative power of both CI was assessed with One-R machine learning model. Correlation between CI and brain connectivity (detected with functional magnetic resonance imagery using seed-based and hypothesis-free intrinsic connectivity) was investigated using a linear regression in a subgroup of 12 UWS and 12 MCS patients with sufficient image quality. Results and Discussion: Significant differences were found between MCS and UWS for CIs and CIl (0.0001≤p≤0.006). Significant correlations were found between CRS-R and CIs and CIl (0.0001≤p≤0.026). The One-R classifier selected CIl as the best discriminator between UWS and MCS with 85% accuracy, 19% false positive rate and 12% false negative rate after a 10-fold cross-validation test. Positive correlations were observed between CI and brain areas belonging to the autonomic system. CI was found to be significantly higher in MCS compared to UWS patients, with high discriminative power and lower false negative rate than the reported misdiagnosis rate of human assessors, providing an easy, inexpensive and non-invasive diagnosis tool. CI is correlated to functional connectivity changes in brain regions belonging to the autonomic nervous system, suggesting that CI can provide an indirect way to screen and monitor connectivity changes in this neural system. Future studies should investigate further the extent of CI’s predictive power for other pathologies in the disorders of consciousness spectrum. [less ▲]

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See detailRelationships between environmental conditions and phytoplankton in the Mellah lagoon (South Western Mediterranean, Algeria), with an emphasis on HABs species
Draredja, Mohamed Anis; Frihi, Hocine; Boualleg, Chahinez et al

Poster (2018, October)

For the first time, a bi-monthly monitoring of environmental parameters (temperature, salinity, nutrients) and phytoplankton assemblages was conducted in the well-preserved Mellah lagoon ecosystem (South ... [more ▼]

For the first time, a bi-monthly monitoring of environmental parameters (temperature, salinity, nutrients) and phytoplankton assemblages was conducted in the well-preserved Mellah lagoon ecosystem (South Western Mediterranean, Algeria). Sampling was performed at 3 stations in 2016. The objective was to study the evolution of the microphytoplankton community with a focus on HABs species in relation with the environmental characteristics. In total, 227 microphytoplankton species belonging mainly to Diatomophyceae (160 species) and Dinophyceae (53 species), were inventoried in the Mellah lagoon. There was a clear dominance of diatoms (63%), compared to dinoflagellates (37%). Proliferations of some potentially toxic species such as Prorocentrum micans (7 200 cells.l-1) and Gymnodinium sanguineum (6 000 cells.l-1), were observed in summer and autumn respectively. Other HABs species as Alexandrium tamarense/catenella (1 350 cells.l-1) and Alexandrium minutum (1 420 cells.l-1), were present in winter and spring respectively. Temporal differences in the qualitative composition and quantitative phytoplankton communities in the three stations are discussed in relation with environmental variables. Microphytoplankton populations in the Mellah show diversity and equitability indices of 3.04 bits.cell-1 and 0.94 respectively, showing a structured community during winter and spring. The rest of the year is characterized by unstable phytoplankton populations, where only opportunistic species develop. [less ▲]

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See detailDéveloppement d’un système de vision multispectrale pour le phénotypage de culture
Naiken, Alwin ULiege; Mercatoris, Benoît ULiege

Poster (2018, October)

In the frame of research activities regarding the characterization of crop growth dynamics in a context of precision agriculture, we have developed a multispectral proxidetection system covering a wide ... [more ▼]

In the frame of research activities regarding the characterization of crop growth dynamics in a context of precision agriculture, we have developed a multispectral proxidetection system covering a wide spectrum in the near-infrared and short-wave infrared domains. This part of the spectrum is important for the characterization of vegetation since it includes the reflectance plateau of plants in the near-infrared and the main water absorption bands. Nowadays, the most commonly used tool in this spectral range is the spectrometer, which measures the average reflectance of a scene without any spatial detail. In the infrared range, hyperspectral imaging is ermerging, as a high-potential solution, but remains difficult to implement and process. In this contribution, we propose a solution, based on an optical filter wheel, which provides the spatial dimension in the infrared domain with a simple implementation to study plants at the scale of organs (leaves, stems, ears). The proposed multispectral vision system complements an equivalent tool already developed in the visible and near-infrared field using a monochrome CMOS camera. The particularity of the presented instrument is the use of an InGaAs camera (indium-galllium arsenide sensor) Gold-eye G-007 (Allied Vision) with a bandwidth from 950 nm to 1650 nm. This camera is combined with a wheel composed of 10 optical filters of 100 nm bandwidth and distributed over the camera's bandwidth. The positioning of the filters in front of the camera is automatically controlled by the image acquisition system using a microcontroller (Arduino Uno). The implemented program allows to select successively the filters of interest for the acquisition. This multipectral vision system will allow the selection of wavelength ranges and morphological parameters that explain physiological traits of plants such as water or nitrogen deficiencies. For this purpose, the image characteristics will be correlated with conventional reference measurements. [less ▲]

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See detailCOMPLETE STATISTICAL WORKFLOW FOR OPTIMIZED USED OF GC×GC-TOFMS
Stefanuto, Pierre-Hugues ULiege; Focant, Jean-François ULiege

Poster (2018, October)

In the last years, multidimensional chromatography methods have undergone numerous developments on the technical side. Several commercial systems of two dimensional gas chromatography coupled to high ... [more ▼]

In the last years, multidimensional chromatography methods have undergone numerous developments on the technical side. Several commercial systems of two dimensional gas chromatography coupled to high-resolution mass spectrometer (GC×GC-HRTOFMS) are now commercially available. However, the users are still facing challenges for method optimization and data processing steps. This research aims to develop a complete optimization and processing workflows for GC×GC-TOFMS application. The simplification of the technique would be a game change for broader transfer of this technology to the industry. [less ▲]

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See detailHow children with Developmental Language Disorders learn lexical categories?
Krzemien, Magali ULiege; Levaux, Emilie ULiege; Maillart, Christelle ULiege

Poster (2018, October)

Developmental Language Disorders (DLD) are linked to a deficit in the generalization of morphosyntactic structures. Children with DLD have difficulty in abstracting constructions schemas and in applying ... [more ▼]

Developmental Language Disorders (DLD) are linked to a deficit in the generalization of morphosyntactic structures. Children with DLD have difficulty in abstracting constructions schemas and in applying them to new instances (Leroy et al., 2014). This could be due to a problem with analogical reasoning and with the comparison of different forms to detect a similar relational structure (Krzemien et al., 2017 ; Leroy et al., 2012). These hypotheses have been investigated in the non-verbal domain and in morphosyntax, but little is known about the generalization of children with DLD in the lexical area. In this study, we examine the first and second order generalization in control and DLD children for new lexical categories. We make the assumption that DLD are linked to an impairment in the ability to generalize new vocabulary. We therefore use new words associated with different categories: there are three types of entity categories (objects, animated objects and non-solid substances) as well as two types of relational categories (relational role and relational object). Children are provided with an exemplar of a new category and are asked to find another one among three solutions. This allows us to evaluate the second order generalization and the acquisition of the pertinent feature (shape, texture, relation, etc.) to use in order to categorize non-familiar referents. If they fail, they are shown two exemplars of this same category and if they fail again, they are shown three exemplars. This permits us to examine the first order generalization and the ability to benefit from the comparison of several exemplars in order to generalize a new word appropriately. Fifteen children with DLD have been recruited and matched to children without language disorders on age and non-verbal intellectual quotient on the one hand, and on a measure of vocabulary comprehension on the other hand. [less ▲]

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See detailWord learning and Statistical Inference in Children with Developmental Language Disorder
Dauvister, Estelle ULiege; Maillart, Christelle ULiege

Poster (2018, October)

Word learning is a language component that usually appears to be impaired in children with developmental language disorders (DLD) (Kan & Windsor, 2010). Despite it has long been studied, the underlying ... [more ▼]

Word learning is a language component that usually appears to be impaired in children with developmental language disorders (DLD) (Kan & Windsor, 2010). Despite it has long been studied, the underlying mechanisms of these difficulties remain unclear. For several years, however, we assist to the emergence of Bayesian learning models, which have been applied to account for word learning process (Perfors, Tenenbaum, Griffiths & Xu, 2011; Xu & Tenenbaum, 2007). According to these models, human beings are equipped with a strong mechanism of probabilistic, inductive inference (as opposed to frequentist approaches) which allows the learning processes to take place. Consider this, our study aims at exploring the hypothesis of a deficit of inductive inference in children with DLD as explaining the lexical difficulties they exhibit. We decided to create a novel word learning task inspired by Xu and Tenenbaum (2007). In this task, sets of one or three objects, which correspond to exemplars of novel words (e.g. dax), are presented to children with DLD. Once exposed to the exemplars, children have to pick out the other objects of the same type (other dax) from a larger set of new objects. Objects from several semantic categories and different levels of taxonomy are proposed. We can then explore the ability of children to learn and generalize through inductive inference by exploring the distributional pattern of responses across categories and levels of taxonomy. The ability of adjustment of the child’s responses given the condition (one or three examples) will also be taken into account in our analyses. [less ▲]

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See detailIdentification of serotypes of enteropathogenic (EPEC) Escherichia coli isolated from diarrheic calves
Habets, Audrey ULiege; Duprez, Jean-Noël ULiege; Iguchi, Atsuchi et al

Poster (2018, October)

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See detailIdentification of unconventional Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli from diarrheic calves.
Habets, Audrey ULiege; Duprez, Jean-Noël ULiege; Iguchi, Atsushi et al

Poster (2018, October)

Introduction Escherichia coli producing the Shiga toxins (STEC) and/or the attaching-effacing (AE) lesion (AE-STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also ... [more ▼]

Introduction Escherichia coli producing the Shiga toxins (STEC) and/or the attaching-effacing (AE) lesion (AE-STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the intestines of healthy cattle. AE-STEC in humans can belong to scores of O serogroups with 7 most frequent and pathogenic ones (O157, O26, O103, O111, O121, O145 and O165). Conversely the majority of AE-STEC in young calves belong to a narrower range of serogroups (O26, O111, O118, O5). Besides AE-STEC, E. coli producing only the AE lesion (EPEC) are also frequently associated with diarrhoea in young calves. Half of them belong to the O26 and O80 serogroups,, but the serotypes of the second half of isolates remain unidentified (Fakih et al. 2017; Thiry et al., 2018). Materials and Methods Different unconventional serogroups (O123/186, O156, O177, O182, O183) were identified by PCR in 5 STEC or EPEC that tested negative for the pre-cited 12 serogroups (Iguchi et al., 2015). The aim of this study was therefore to identify those 5 serogroups and the O146 serogroup amongst 76 AE-STEC, STEC and EPEC isolated between 2008 and 2015 from diarrheic calves at ARSIA (Fakih et al., 2017). Two triplex PCRs were applied either for the O146_O182_O183 serogroups or for the O123/186_O156_O177 serogroups. Discussion So far the first triplex PCR identified 4 O182-positive and 2 O183-positive STEC and EPEC. These 6 PCR-positive isolates were confirmed by either uniplex PCR. Two O182-positive isolates were AE-STEC and 2 were EPEC whereas the two O183-positive isolate were STEC. The second triplex PCR identified 10 O123-186-positive, 2 O156-positive and 13 O177-positive STEC and EPEC. These 25 PCR-positive isolates were confirmed by the three uniplex PCR. One O123/186-positive isolate was AE-STEC and 9 were EPEC. One O156-positive isolate was AE-STEC and one was STEC and finally, 12 O177-positive isolates were EPEC and 1 was STEC. The further steps of the work are: (i) the identification of the same 6 serogroups amongst a collection of (AE-)STEC and EPEC isolated from healthy cattle at slaughterhouse; (ii) the identification of still other serogroups amongst the remaining untyped calf (AE-)STEC and EPEC; (iii) the comparison of the calf-positive STEC and EPEC with human (AE-)STEC and EPEC belonging to the same serogroups. References Fakih, I., Thiry, D., Duprez, J. N., Saulmont, M., Iguchi, A., Piérard, D., … Mainil, J. G. (2017). Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC. Veterinary Microbiology, 202, 16–22. Iguchi, A., Iyoda, S., Seto, K., Morita-Ishihara, T., Scheutz, F., & Ohnishi, M. (2015). Escherichia coli O-genotyping PCR: A comprehensive and practical platform for molecular O serogrouping. Journal of Clinical Microbiology, 53(8), 2427–2432. https://doi.org/10.1128/JCM.00321-15 Thiry, D., De Rauw, K., Takaki, S., Duprez, J. N., Iguchi, A., Piérard, D., … Mainil, J. G. (2018). Low prevalence of the “gang of seven” and absence of the O80:H2 serotypes among Shigatoxigenic and enteropathogenic Escherichia coli (STEC and EPEC) in intestinal contents of healthy cattle at two slaughterhouses in Belgium in 2014. Journal of Applied Microbiology, 124(3), 867–873. [less ▲]

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See detailCross-sectional swallowing study in patients with disorders of consciousness
MELOTTE, Evelyne ULiege; Delhalle, Sabrina; Gosseries, Olivia ULiege et al

Poster (2018, September 29)

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See detailValidation of 24 blood gas analyzers, GEM 5000 Premier
GADISSEUR, Romy ULiege; MUSSO, Giuseppe ULiege; Cavalier, Etienne ULiege et al

Poster (2018, September 28)

Introduction: Point-of-care blood gas test results may help to take therapeutic decision by their immediate impact on patient care. Recently, a novel cartridge-type blood gas analyzer, the GEM Premier ... [more ▼]

Introduction: Point-of-care blood gas test results may help to take therapeutic decision by their immediate impact on patient care. Recently, a novel cartridge-type blood gas analyzer, the GEM Premier 5000 (IL-Werfen) was commercialized and 24 analyzers were installed at the University Hospital of Liège. One of them was installed in the main central laboratory, the 23 other ones were installed into 19 patient care units. Before the implementation, we evaluated the analytical performance of all the 24 GEM Premier 5000, for the determination of whole blood pH, pCO2 and pO2, electrolytes (Na+, K+, Cl−), ionized calcium (iCa2+), glucose, lactate and co-oximetry parameters ((total hemoglobin (tHb), oxyhemoglobin (O2Hb), carboxyhemoglobin (COHb), methemoglobin (metHb), deoxyhemoglobin (HHb)). Method: First, we evaluated the performance of the GEM Premier 5000 dedicated to the central lab, so-called “referent analyzer”, with 3 levels of External Quality Controls material (EQC RNA Medicals). CLSI EP5 recommends 2 replicates per run, 1 or 2 runs per day, for a minimum of 20 days. Nevertheless, we analyzed the 3 levels of EQC, 2 replicates per run, 2 runs per day during 5 consecutive days. Afterwards, on the whole 24 GEM Premier 5000, we analyzed aqueous QC material (Werfen GEM System Evaluator, level 1-2), 3 replicates within a single run, once per day, during 5 consecutive days. We determined the manufacturer's claim for Within-Run and Total precisions for each. Co-oxymetry parameters were not evaluated on 4 analyzers. Then, we compared the all 23 analyzers to the “referent analyzer” of the central lab. Therefore, for each parameter, we showed in a Youden diagram all the results obtained by 23 analyzers. The position of the acceptance ranges were shown graphically using the specifications for Acceptable(%) Root Mean Standard Deviation (RMSD) proposed by the German Guidelines for Quality (RILIBAK) for whole blood parameter, for different ranges of parameters. Results: The results showed a good correlation between analyzers excepted for some parameters. Lactate and MetHb: level 2 were often over-estimated when compared to “Reference Analyzer”. It could be explained by the fact that this IQC level contains very low Lactate and MetHb rates. The pO2 level2: some results were over estimated (random errors >< cassette reagents >< low values). The pO2 level1: over-estimated with 8 analyzers letting us think that the cassette reagent of our “Reference Analyzer” had a bias in the lower range. Conclusion: Performance evaluation of a large cluster of Blood Gas Analyzers is always a challenge for a Hospital. Accreditation is one of the main goal in each Belgian laboratory. Hospital Accreditation is also discussed in Belgium. This study shows an interesting approach to validate Blood Gas Analyzers for highlighting data. Based on our study results, we estimated that the evaluated instrument are a suitable blood gas analyzer for both POCT and laboratory use. [less ▲]

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See detailLa "Vigna Manzi" a Garaguso (MT). Risultati dello scavo del 2008
Cuvelier, Graham ULiege

Poster (2018, September 27)

Ce poster, exposé dans le cadre du colloque international organisé annuellement par l'Istituto per la Storia e l'Archeologia della Magna Grecia, présente les résultats de la fouille archéologique menée en ... [more ▼]

Ce poster, exposé dans le cadre du colloque international organisé annuellement par l'Istituto per la Storia e l'Archeologia della Magna Grecia, présente les résultats de la fouille archéologique menée en 2008 sur le site de la "Vigna Manzi" à Garaguso. [less ▲]

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See detailImpact of age and napping on actimetry-derived sleep and 24-h rest-activity indices
Reyt, Mathilde ULiege; Latteur, Alizée; Maire, Micheline et al

Poster (2018, September 26)

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See detailHow could the herbicidal effects of selected essential oil compounds be related to their membrane activity ?
Lins, Laurence ULiege; Bettaieb, Ines; Dal Maso, Simon ULiege et al

Poster (2018, September 25)

The European legislation on plant protection products (PPP) is about to undergo important changes in the coming years. The chemical PPP destined to be removed from the European market are responsible for ... [more ▼]

The European legislation on plant protection products (PPP) is about to undergo important changes in the coming years. The chemical PPP destined to be removed from the European market are responsible for the appearance of resistance phenomena to plants pests. It is therefore necessary to explore new alternatives, one of them being the search for natural herbicides. Essential oils (EO) could provide a welcome alternative due to their proven activity as PPP. Even if these compounds seem to have a bright future as PPP, their activity is mainly approached through empirical observations. EO are also a complex mix of of different molecules that could act individually or in synergy. Particularly, very little is known when it comes to molecular mechanisms of action and the relations between structure and activity of the active compounds. This study aims to investigate the structure/activity relationships of some EO molecules, among which cinnamaldehyde (CIN) from cinnamon and citronellal and citronellol from lemongrass. Due to their lipophilic properties, EO tend to interact with one or more of the layers found in the outer plant tissues, among which the cell membranes. For cinnalmaldehyde (and other EO compounds), it has been shown that they are able to interact with bacterial phospholipids and induce change in lipid organization (fluidity, packing,..) on model lipid monolayers (Nowotarska et al, 2014). Citronnellol was notably shown to displace cholesterol from its phospholipid complexes (Lange, Y et al, 2009). However, nothing is known about any interaction with lipids specific to plant plasma membrane (PPM). In this study, we analyzed the effects of the above three EO compounds on model PPM by complementary in vitro and in silico biophysical approaches. We showed that the three compounds have differential effects on plant lipids and different herbicidal properties on plantae. While part of the herbicidal activity could be related to membrane perturbation, some clues remain to be elucidated. Future studies at a molecular point of view would help to better decipher the herbicidal action involving the membrane, other outer plant tissues such as the cuticule and/or and a potential effect on EO compounds on proteins or genomic DNA, as it was shown for CIN on E. Coli (He, TF et al, 2018). [less ▲]

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See detailSpatio-temporal floral resource shifts in Belgium
Jacquemin, Floriane ULiege; Violle, Cyrille; Coppee, Thomas ULiege et al

Poster (2018, September 20)

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See detailA multi-stressor analysis of spatio-temporal shifts in Belgian bee community
Rollin, Orianne ULiege; Michez, Denis; Jacquemin, Floriane ULiege et al

Poster (2018, September 20)

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See detailNonlinear Real-Time Emulation of a Guitar Chain with Machine learning techniques
Schmitz, Thomas ULiege

Poster (2018, September 19)

Numerous audio systems for musicians are expensive and bulky. Therefore, it could be advantageous to model them and to replace them by computer emulation. Their nonlinear behavior requires the use of ... [more ▼]

Numerous audio systems for musicians are expensive and bulky. Therefore, it could be advantageous to model them and to replace them by computer emulation. Their nonlinear behavior requires the use of complex models. Recent progresses made in the nonlinear system identification field have improved the ability to emulate nonlinear audio systems such as tube guitar amplifiers. [less ▲]

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See detailAntiinflammatory and antileukemic-like activities of R01Yob, a medicinal plant of the rwandese pharmacopeia
Tomani, J; Nyirimigabo, Alain; Tchouate, O et al

Poster (2018, September 19)

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See detailMars emissions from CO and CO2+: IUVS-MAVEN limb observations and model
Gérard, Jean-Claude ULiege; Gkouvelis, Leonardos ULiege; Ritter, Birgit ULiege et al

Poster (2018, September 17)

The IUVS Ultraviolet spectrograph (McClintock et al. 2014) on board MAVEN has been collecting thousands of airglow (Jain et al. 2015) or auroral (Schneider et al., 2015) limb profiles in the range 120 to ... [more ▼]

The IUVS Ultraviolet spectrograph (McClintock et al. 2014) on board MAVEN has been collecting thousands of airglow (Jain et al. 2015) or auroral (Schneider et al., 2015) limb profiles in the range 120 to 340 nm (Fig. 1) since November 2014. We have analyzed more that three years of airglow observations and compared them to model simulations. MAVEN has been quasi-continuously collecting airglow observations since November 2014, covering more than 1.5 Martian year and various latitudes ranges. The main features are emissions from CO, CO2+, O, N2 and C. In this work, we compare the characteristics of the CO2+ ultraviolet doublet (UVD) limb profiles with model simulations. From this comparison, we derive the CO2 column density above the 120-130 km region. [less ▲]

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See detailAnalysis of the lung microbiota in healthy dogs and in canine idiopathic pulmonary fibrosis, a possible spontaneous model for human IPF.
Fastrès, Aline ULiege; Roels, Elodie ULiege; Taminiau, Bernard ULiege et al

Poster (2018, September 16)

Canine idiopathic pulmonary fibrosis (CIPF) mimics human IPF and affects old dogs from one breed: the West Highland white terrier (WHWT). In human IPF, lung microbiota is suspected to be associated with ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) mimics human IPF and affects old dogs from one breed: the West Highland white terrier (WHWT). In human IPF, lung microbiota is suspected to be associated with disease pathogenesis and might serve as a therapeutic target. The aim of the study was to characterize microbial alteration associated with breed, environment and CIPF disease. Bronchoalveolar lavage fluid was sampled from 4 groups of dogs: client-owned WHWTs affected with CIPF from Belgium-BE (n=7, 11.6y), healthy client-owned BE-WHWTs (n=5, 11.2y), healthy client-owned WHWTs from Finland-FI (n=5, 11.0y) and healthy experimental BE-beagle dogs (n=6, 8.8y). Metagenetic analysis was performed on V1-V3 hypervariable region of 16S rDNA after total bacterial DNA extraction and sequencing on a MiSeq Illumina sequencer. Data were compared between healthy BE-WHWTs and BE-beagles, healthy BE-WHWTs and FI-WHWTs, and healthy BE-WHWTs and CIPF BE-WHWTs. Data analyses demonstrated that the same phyla (Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes) predominated in all groups. Significant differences (p<005) were mainly noted for species with relative abundance of less than 1%, except for Micrococcus luteus, higher in FI-WHWTs (7.4%) compared to BE-WHWTs (0%) and for Pseudomonas JF766687 and EU373411, Serratia and Hydrogenophilus spp, higher in BE-beagles (15.3, 8.1, 5.0 and 1.5%) compared with BE-WHWTs (0.3, 0.3, 0, 0.1%, respectively). Results indicate that further analysis of the impact of breed or environment and inclusion of larger amounts of dogs are needed before investigation of lung microbiota as a biomarker or therapeutic target in CIPF. [less ▲]

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See detailBCCM/ULC: a unique Biological Resource Center of (sub)polar cyanobacteria
Santoro, Mariano ULiege; Beets, Kim ULiege; Lara, Yannick ULiege et al

Poster (2018, September 13)

The BCCM/ULC public collection of cyanobacteria funded by the Belgian Science Policy Office (BELSPO) focusses on the ‘ex situ’ conservation of a representative portion of the (sub)polar cyanobacterial ... [more ▼]

The BCCM/ULC public collection of cyanobacteria funded by the Belgian Science Policy Office (BELSPO) focusses on the ‘ex situ’ conservation of a representative portion of the (sub)polar cyanobacterial diversity with different origins, isolated from terrestrial (e.g. soil crusts, cryoconites, endoliths) and aquatic ecosystems (e.g. limnetic microbial mats, freshwater lakes and marine environments). BCCM/ULC currently holds 175 cyanobacterial strains, including over 100 of polar origin (catalogue: http://bccm.belspo.be/catalogues/ulc-catalogue-search). The strains are available for researchers who study the taxonomy, evolution, biogeography, adaptation to harsh environmental conditions, etc. Morphological and molecular identifications (based on SSU rRNA sequences) indicate that the strains belong to the orders Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales. This large taxonomic distribution makes the collection interesting for phylogenomic and genomic make-up studies, hence the genome sequencing of several strains is ongoing. Continuous maintenance of living cultures ensures the preservation of strains, whose majority are cryopreserved (as back-up at -70°C) in order to limit the genetic drift. BCCM/ULC obtained an ISO 9001:2015 certification for public and safe deposits, and distributions of strains, as part of the multi-site certification for the Belgian Coordinated Collections of Microorganisms (BCCM) consortium. The policies of acquisition and distribution of the collection are translated respectively into contracts called Material Accession Agreements (MAA) and Material Transfer Agreements (MTA). This guarantees safe fit-for-use microbiological material and data compliant with the rules on access and utilization of the Nagoya Protocol under the Convention on Biological Diversity (12 October 2014). BCCM/ULC progressively incorporates the most interesting strains from the research collection of the host laboratory into the public collection, whose variety is also enriched by public deposits from other geographical areas (more temperate). The collection is also interested to test new cultivation methods to better reproduce the complex ecological interactions experienced in nature. In addition, Antarctic cyanobacterial strains are known to produce a range of secondary metabolites with different potential bioactivities, as well as the exploration of some unknown gene clusters identified in the first Antarctic cyanobacterial genome ever determined may potentially lead to discover novel peptides which could have biotechnological or biomedical applications. [less ▲]

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See detailImpact of contextual novelty on memory performance of patients with amnestic mild cognitive impairment
Marcotte, Coralie ULiege; Delhaye, Emma ULiege; Besson, Gabriel ULiege et al

Poster (2018, September 13)

Novelty detection and processing play a critical role in memory, so that new information is better remembered than familiar information. However, few studies have been conducted on how novelty influences ... [more ▼]

Novelty detection and processing play a critical role in memory, so that new information is better remembered than familiar information. However, few studies have been conducted on how novelty influences the functioning of memory in Alzheimer's disease (AD) and more specifically its early stage, Mild Cognitive Impairment (MCI). The purpose of this study was to explore the impact of contextual novelty detection and processing on MCI patients’ memory. Twenty healthy older participants and 20 MCI patients performed a task based on the von Restorff paradigm. This task consisted of 25 lists of 10 words. For 20 lists, one word was salient because it had a larger font size (60 for non-target words and 90, 120 or 150 for target words). After each list, participants had to perform a free recall task. The effect of novelty (or VR effect) was calculated by a VRratio based on the percentage of target words recalled and the percentage of non-target words recalled. We also collected neuroimaging data to determine if there is a correlation between hippocampal size and the effect of novelty, given that the hippocampus is atrophied in MCI patients and typically involved in the processing of novelty. The analysis of VRratio (ANOVA 2 (groups) x 3 (font size) with repeated measures on the last variable) showed an effect of the font size but no effect of groups. Participants recalled more target words written in font size 120 and 150 compared to font size 90. Furthermore, Pearson correlation analysis demonstrated a positive correlation between the novelty effect for font size 120 and the right and left hippocampal volumes. This study indicates that MCI patients are able to detect and process novelty, at least contextual novelty as assessed here. However, novelty did not allow to normalise the memory performance of MCI patients since they still recalled fewer target words than healthy older adults. In addition, the ability to benefit from novelty to improve memory appears to be related to hippocampal volume. [less ▲]

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See detailSubjective feeling of fatigue and cognitive performances: a specific link in Multiple Sclerosis
Guillemin, Camille ULiege; LOMMERS, Emilie ULiege; DELRUE, Gaël ULiege et al

Poster (2018, September 13)

Introduction. Fatigue is one of the most frequent and debilitating symptom reported by patients with Multiple Sclerosis (MS). Yet, the link between cognitive impairments and subjective feeling of fatigue ... [more ▼]

Introduction. Fatigue is one of the most frequent and debilitating symptom reported by patients with Multiple Sclerosis (MS). Yet, the link between cognitive impairments and subjective feeling of fatigue remains unclear. This study aims to investigate the associations between cognitive performance and subjective measures of both physical and mental fatigue. Stepwise regression analyses were conducted in order to determine, correspondingly, the contribution of physical and cognitive fatigue scores, as well as depression and anxiety scores, on patients' cognitive performance. Methods. 38 MS patients (EDSS: 1 to 6) and 28 healthy controls, matched on age and sex, were included in this study. Cognitive and physical fatigue scores were obtained from the Modified-Fatigue Impact Scale (M-FIScog and M-FISphys), and anxiety and depression level were determined from the HAD scale. Cognitive performance was assessed for working memory (Working Memory Index (WMI) from the WAIS III), verbal episodic memory (sum of the 5 learning trials of the CVLT), visual episodic memory (immediate recall score of the 10/36 test), processing speed (Processing Speed Index (PSI) from the WAIS-IV, Symbol Digit Modality Test (SDMT), denomination and reading speed at the Stroop task) and executive functioning (interference score of the Stroop task). In order to take participants' age distribution and education levels into account, cognitive scores were converted into standard scores. Results. For MS patients, M-FIScog scores explained variance in performance for PSI (R² :0.12, p<0.05), and for denomination (R² : 0.17, p<0.05) and interference (R² :0.32, p<0.0005) scores of the Stroop task. Regarding the control group, no variable was significant for each 8 cognitive scores. M-FISphys, depression and anxiety scores were retained by none of the significant model. Discussion. These results stress the need to dissociate cognitive and physical fatigue in MS. Only cognitive fatigue appeared as an explanatory variable of cognitive performance. Moreover, the influence of cognitive fatigue seems to be MS-specific for task relying on processing speed, and accentuated when the task requires executive control. [less ▲]

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See detailImpact of the number of alternatives in a forced choice recognition memory task on performance in normal aging
Marcotte, Coralie ULiege; Simon, Jessica ULiege; Gilsoul, Jessica ULiege et al

Poster (2018, September 13)

Forced-choice recognition memory tasks are often used to test visual episodic memory, notably to assess the effect of age on memory performance. However, no study has examined the impact of the number of ... [more ▼]

Forced-choice recognition memory tasks are often used to test visual episodic memory, notably to assess the effect of age on memory performance. However, no study has examined the impact of the number of alternatives on memory performance. In this experiment, we evaluated whether memory performance of young and older participants is influenced by, on the one hand, the number of proposed alternatives - two or three - and on the other hand the degree of similarity between the target and its lures. The study included 48 young participants and 43 healthy older participants. During the encoding phase, they saw 36 photographs of faces twice. During the recognition task, we asked them to choose the previously presented face among two or three photographs. Half of the target-lure sets were more similar than the other half (60% of common characteristics versus 40%). After the memory task, participants completed the Cambridge Face Perception Test to measure their capacity of perception and processing of faces. The analysis of correct recognition responses (ANOVA 2 (groups) x 2 (alternatives) x 2 (similarity) with repeated measures on the last two variables) showed that older adults had poorer performance than young adults and an effect of the degree of similarity but no effect of the number of alternatives. Interestingly, the age effect on recognition performance disappeared when controlling for face perception abilities. Thus, part of the difficulties in visual recognition memory for faces of older participants could be underpinned by weak perceptual capacities. [less ▲]

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See detailDevelopment and Validation of the Simultaneous Measurement of Estrone and 17β-Estradiol in Serum by LC-MS/MS for Clinical Laboratory Applications
Pitarch-Motellón, Jorge; Cavalier, Etienne ULiege; LE GOFF, Caroline ULiege et al

Poster (2018, September 12)

A straightforward analytical method for the determination of estrone (E1) and 17β-estradiol (E2) at ultra-low levels in serum samples by LC-MS/MS has been developed and validated. This method entails an ... [more ▼]

A straightforward analytical method for the determination of estrone (E1) and 17β-estradiol (E2) at ultra-low levels in serum samples by LC-MS/MS has been developed and validated. This method entails an extraction and derivatization with dansyl chloride followed by separation with a C18 column. A comparison of the developed LC-MS/MS method against our routine immunoassay shows a good correlation for E2 while an important negative bias is observed for E1 by RIA. The later comparison for E1 shows the need to switch from the current routine automated immunoassays to highly-sensitive LC-MS/MS quantifications in order to provide accurate and reliable clinical results, especially at very low levels. [less ▲]

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See detailCortisol everywhere!
Arias-Carnero, Benjamin; PEETERS, Stéphanie ULiege; DEVILLE, Marine ULiege et al

Poster (2018, September 12)

Our problem with a routine LC-MS/MS system is related to the presence of an isobaric interference of cortisol from an unknown source. After cleaning and checking the proper function of the LC system, we ... [more ▼]

Our problem with a routine LC-MS/MS system is related to the presence of an isobaric interference of cortisol from an unknown source. After cleaning and checking the proper function of the LC system, we performed an exhaustive study of all the possible contamination sources that can affect the method performance. Bovine Serum Albumin (BSA) seems the critical reactive that contained the highest amount of this interference, among other sources tested. Identity of this compound was obtained by QTOF analysis and resulted to be cortisol. [less ▲]

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See detailCXCL12-loaded poly(lactic-co-glycolic) acid microspheres for the chemotactic recruitment of glioblastoma stem cells
Lumapat, Paul Noel ULiege; Garcia Fuentes, Marcos; Csaba, Noemi et al

Poster (2018, September 12)

INTRODUCTION Glioblastoma (GBM) stem cells (GSC) have been found to specifically migrate in response to a gradient of CXCL12 in a CXCR4-dependent manner1. This enables the escape of GSCs from the tumor ... [more ▼]

INTRODUCTION Glioblastoma (GBM) stem cells (GSC) have been found to specifically migrate in response to a gradient of CXCL12 in a CXCR4-dependent manner1. This enables the escape of GSCs from the tumor mass, potentially evading treatment and initiating metastases. We seek to take advantage of this mechanism for GBM therapy. By encapsulating CXCL12 in poly(lactic-co-glycolic) acid (PLGA) microspheres, we aim to create a polymeric platform capable of recruiting and directing migratory GSCs, thereby influencing GBM progression and metastasis formation. METHODS Human CXCL12 was initially complexated with heparin and poloxamine (Tetronic 1107)2. Resulting nanocomplexes were encapsulated in PLGA via emulsion solvent evaporation/extraction to form microspheres. Microspheres were characterized for morphology, encapsulation efficiency, and in vitro release characteristics. To verify promigratory activity, media preconditioned with blank and CXCL12-loaded microspheres were evaluated for chemotactic activity on U87MG GBM cells using a transwell migration assay. RESULTS AND DISCUSSION CXCL12-heparin nanocomplexes were successfully encapsulated in PLGA microspheres with diameter of 81.9±58.3 µm. The formulation had low initial burst release in vitro at <8%, and released >40% of the payload over a period of up to 90 days. Furthermore, media preconditioned with CXCL12-loaded microspheres for 1 to 8 weeks displayed promigratory activity towards GSCs. The number of migrating cells were 1.8- to 2.8-fold higher as compared to media preconditioned with blank microspheres. Treatment with AMD3100, a CXCR4 antagonist, abrogated this promigratory effect, indicating CXCR4 involvement. CONCLUSION The obtained results point to the potential of CXCL12-loaded microspheres for long-term recruitment of GSCs. These chemotactic microspheres, in combination with conventional and/or novel therapies, present a promising strategy for tackling GBM and its recurrence. [less ▲]

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See detailRestoring a worn-out pasture : What impact on greenhouse gas exchanges ?
Lognoul, Margaux ULiege; Gourlez de la Motte, Louis ULiege; Debacq, Alain ULiege et al

Poster (2018, September 12)

The restoration of permanent pastures is often required in order to restore a productive state and the palatability of the grass. The restoration process consists on destroying the former vegetation using ... [more ▼]

The restoration of permanent pastures is often required in order to restore a productive state and the palatability of the grass. The restoration process consists on destroying the former vegetation using herbicides followed by tillage and reseed. The short term and long term impacts of such operations on the carbon cycle and N2O emissions are not well defined for old permanent pastures. Therefore, a paired flux tower measurement campaign was started in March 2018 at the Dorinne Terrestrial Observatory in Southern Belgium, with the aim to study the impact of pasture restoration on CO2 and N2O fluxes exchanged by the ecosystem. The site is a 100-year-old intensively managed grassland which last restoration was performed more than 40 years ago. It is grazed by Belgian blue beef cattle and fertilized with around 120 kgN ha-1 per year on average, reflecting common practices in the area. A former study carried out at the same site, showed that the pasture acted as significant carbon sink before the start of experiment. Two adjacent parcels belonging to the same farm were both equipped with identical instrumentation including eddy covariance measurements of CO2 (LICOR 7000) and N2O/CH4 (Aerodyne Inc. quantum cascade laser) exchanges to allow the comparison between a control and a restored plot subject to identical pedo-climatic conditions. Preliminary results of greenhouse gas fluxes will be presented in relation to climatic conditions and management operations and the evolution of soil ammonium and nitrate. [less ▲]

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See detailN2O flux short-term response to meteorological solicitations and farming practices in a fertilized crop
Lognoul, Margaux ULiege; Debacq, Alain ULiege; Manise, Tanguy ULiege et al

Poster (2018, September 11)

Using the eddy covariance technique, half-hourly N2O fluxes were measured over a sugar beet crop (Terrestrial Observatory of Lonzée, BE, ICOS site level 2) between March and October 2016. Several ... [more ▼]

Using the eddy covariance technique, half-hourly N2O fluxes were measured over a sugar beet crop (Terrestrial Observatory of Lonzée, BE, ICOS site level 2) between March and October 2016. Several parameters of data quality control tests were modified to suit the characteristics of N2O. The u* filtering threshold was determined based on CO2 data as the procedure could not be implemented using N2O fluxes. The uncertainty on N2O fluxes was assessed for several aspects of data treatment (total random uncertainty, spectral correction, u* filtering, gap-filling), which were combined to determine the uncertainty on the budget. N2O flux variability was characterized by three peak episodes during the experiment, interspersed with background fluxes. These events were driven by several variables, depending on the time-scale. The more time had passed after fertilization, the lower the potential for high fluxes was, and by the end of the crop season, only background flux was recorded. The soil water content at 5 cm was identify as the single trigger of N2O emission bursts, while intraday oscillations were positively correlated to the variations of surface temperature. For the first time, an inhibiting effect of surface soil disturbance (seed-bed preparation) on N2O fluxes was observed, which delayed the start of the following emission peak. This observation combined to the synchronicity between surface temperature and the oscillations of N2O fluxes supports the hypothesis of a N2O producing microbial community located in the topmost soil layer. Between fertilization and harvest, the crop emitted 6520 (± 908) μmol N2O m-2 which corresponds to an EF of 1.3 % - slightly above the IPCC estimate. Our results stress the importance of measuring N2O exchanges in fertilized crops, as it weighed for 20% of the GHG budget. [less ▲]

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See detailApsûGIS : a GIS-tool for groundwater vulnerability assessment using physical criteria
Dollé, Fabien ULiege; Thomas, Caroline ULiege; Orban, Philippe ULiege et al

Poster (2018, September 11)

The Apsû method has been developed since 2004 to produce groundwater intrinsic and specific vulnerability maps based on a quantitative description of contaminant transport processes in the subsurface. It ... [more ▼]

The Apsû method has been developed since 2004 to produce groundwater intrinsic and specific vulnerability maps based on a quantitative description of contaminant transport processes in the subsurface. It is based on the source-pathway-receptor approach, driven by two concepts: land surface dangerosity that accounts for lateral flow on land surface and infiltration and subsurface attenuation capacity. Vulnerability classes are based on physically-based criteria reflecting the sensitivity of groundwater to pollution events, namely contaminant travel time across the unsaturated zone, pollution duration, or contaminant concentration or mass recovery factors at the groundwater table. To facilitate the application of the Apsû method to case studies, GIS-based applications and interfaces have been developed to integrate geodatabases and user interfaces in the same environment software. The main geodatabase contains all the data required for the calculation of the vulnerability coefficient, such as spatial data (topography, land cover, hydrological network, meteorology, soil types…) and specific databases on contaminant properties (i.e. Koc values, degradation constants…), on hydrogeological properties of geological layers constituting the unsaturated zone flow path. Specific user interfaces have been developed to prepare and export spatial data required by the Apsû calculations. Once the groundwater vulnerability factors are calculated, another user interface is available to automatically create and customize different thematic layers related to groundwater vulnerability mapping. This integrated system makes it possible to automate numerous data formatting geoprocessing operations, and to ensure data integrity by minimizing user interactions with the data (spatial frame, data projection, link between spatial data and databases, etc.) and to focus work on the specificities of the areas studied and the interpretation of the vulnerability maps created. The objective of the communication is to describe the general organisation and operation of the GIS-based interface, illustrated with groundwater vulnerability case studies for aquifers of the Walloon Region of Belgium. [less ▲]

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See detailDirect single well measurement of groundwater flux in permafrost-impacted aquifers in Nunavik, Canada
Jamin, Pierre ULiege; Cochand, Marion; Dagenais, Sophie et al

Poster (2018, September 10)

Permafrost dynamics in high latitude territories is a complex process resulting from atmospheric, soil, water and vegetation interaction. Advective heat transport by groundwater has been identified as ... [more ▼]

Permafrost dynamics in high latitude territories is a complex process resulting from atmospheric, soil, water and vegetation interaction. Advective heat transport by groundwater has been identified as potentially playing a significant role in permafrost dynamics. However, there is a lack of direct measurements of groundwater parameters such as hydraulic head and hydraulic conductivity for determining flow patterns and groundwater fluxes in permafrost environments due to difficulties in accessing these remote territories and associated high costs of field work in such remote areas. Existing monitoring wells are often insufficient to allow the assessment of representative hydraulic gradients and realistic groundwater flow rates. Here, the Finite Volume Point Dilution Method (FVPDM) is applied to measure in-situ groundwater fluxes in a supra- and sub-permafrost sandy aquifer within a small watershed in Umiujaq, Nunavik, Canada. The advantage of the FVPDM method is to provide direct measurement of groundwater flux using a single well technique. The tests show that this method can be successfully applied in remote conditions and with limited resources. Darcy fluxes derived from the FVPDM tests varied from 0.577 to 0.840 m/d, implying that advective heat transport from groundwater flow could be contributing to permafrost thaw at this site. These data are important since very few estimates of groundwater fluxes are available in the discontinuous permafrost zone and that such a significant groundwater flux can have a major impact on heat exchange between groundwater and permafrost. Moreover, they are essential for building and calibrating realistic groundwater flow and heat transport models required for better understand permafrost dynamics and sustainable groundwater management in cold environments. [less ▲]

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Peer Reviewed
See detailInvestigation of serum Krebs Von den Lungen 6 concentration as a predisposing factor and in the diagnosis of canine idiopathic pulmonary fibrosis in the West Highland white terrier.
Fastrès, Aline ULiege; Roels, Elodie ULiege; Bolen, Géraldine ULiege et al

Poster (2018, September 06)

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose early and follow the course of pulmonary fibrosis, numerous biomarkers have been studied in both species. In humans, the mucin Krebs Von den Lungen 6 (KL-6) has consistently been found to be increased in the serum from patients with interstitial lung disease, including IPF and is possibly involved in the IPF pathophysiological process. In CIPF, it has not yet been assessed. In this study, we assessed the diagnostic value of serum KL-6 concentration in CIPF as well as the association between serum KL-6 concentrations and breed predisposition for the disease. Blood was obtained from 5 groups of dogs: WHWTs affected with CIPF (n=20, 14 females (F)/6 males (M), mean age=11.0y, range [5.2-14.5]), healthy aged-matched WHWTs (n=18, 11F/7M, 10.5y [5.3-15.3]), and healthy dogs from other breeds not predisposed for CIPF: Jack Russel terriers (JR) (n=13, 8F/5M, 6.0y [3.0-9.1]), Malinois Belgian shepherds (MBS) (n=13, 5F/8M, 6.5y [2.8-10.8]) and King Charles spaniels (KCS) (n=13, 9F/4M, 6.5y [3.6-9.3]). Serum was stored at -80°C until batched analysis. KL-6 concentration was measured with a commercially available ELISA test (Canine Krebs Von den Lungen 6 Elisa kit, amsbio). Results were compared using a covariance analysis including the 5 groups defined above, gender and an interaction between groups and gender together with the effect of the age. The analysis was followed by post-hoc comparisons between groups using t-tests. Results show that only the effect of groups was significant (p=0.010). Serum KL-6 concentration was higher in healthy WHWTs (4.8+/-4.0 ng/ml) compared with KCS (1.6+/-1.8 ng/ml) and MBS (2.2+/-1.0 ng/ml) (p=0.001 and 0.013 respectively) and higher in JR (4.1+/-2.9 ng/ml) compared with KCS (p=0.016). There was no difference between CIPF (3.2+/-1.9 ng/ml) and healthy WHWTs (p=0.072). In conclusion, differences in serum KL-6 concentrations were found amongst breeds in healthy dogs, with higher concentrations observed in terrier breeds. Whether this may reflect a predisposing factor for CIPF development merits further investigations. Unlike what has been found in human IPF, KL-6 is not a good serum biomarker for diagnosis as there was no difference between CIPF and unaffected WHWTs. [less ▲]

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See detailThe invasive potential of introduced exotic trees: what do arboreta tell us ?
Fanal, Aurore ULiege; Mahy, Grégory ULiege; Monty, Arnaud ULiege

Poster (2018, September 05)

An increasing number of woody species are being listed as invasive in Europe. Forestry is the second largest pathway of invasive tree introductions and it is likely that climate change will encourage ... [more ▼]

An increasing number of woody species are being listed as invasive in Europe. Forestry is the second largest pathway of invasive tree introductions and it is likely that climate change will encourage forest managers to plant exotic tree species to maintain wood production. In the early 1900’s, several arboreta were established in Southern Belgium to assess the wood production potential of prospective exotic trees. However, they also offer the unique opportunity to assess the potential invasiveness of exotic tree species. A systematic sampling method was used to conduct surveys in eight arboreta and a buffer zone surrounding them. Regeneration of all exotic trees was recorded as well as biotic (herbaceous competition, composition of the tree stand) and environmental variables (soil type, pH, thickness of litter, canopy closure and climate). A descriptive approach allowed as to identify species showing an abundant regeneration. Linear regressions were implemented to assess whether the patterns in the regeneration of these exotic trees could be explained by their functional traits, dispersal modes, and environmental tolerances. Results revealed that several coniferous species from the North-American West coast exhibit rapid regeneration and/or dispersal, including Tsuga heterophylla, Abies grandis, Chamaecyparis lawsoniana, Pseudotsuga menziesii and Thuya plicata. We therefore recommend to exercise caution when planting these species in future forestry trials given their potentially invasive characteristics. [less ▲]

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See detailGli scavi della "Vigna Manzi". 1916 - Vittorio Di Cicco
Cuvelier, Graham ULiege

Poster (2018, September 04)

Ce poster, exposé dans le nouveau musée du Palazzo Revertera à Garaguso, présente la fouille archéologique menée par Vittorio Di Cicco en 1916 sur le site de la "Vigna Manzi".

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See detailGli scavi della "Vigna Manzi". 1969-1970 - Jean-Paul Morel
Cuvelier, Graham ULiege

Poster (2018, September 04)

Ce poster, exposé dans le nouveau musée du Palazzo Revertera à Garaguso, présente les fouilles archéologiques menées par Jean-Paul Morel en 1969 et 1970 sur le site de la "Vigna Manzi".

Detailed reference viewed: 22 (1 ULiège)