Article (Scientific journals)Biochemistry, biophysics & molecular biologyChemistry > General ChemistryBiochemistry > BiochemistryOpen AccessPeer Reviewed verified by ORBiResearch Concerted Action (IDEA project) of the University of Liège (ULG)CEE (FP7 network: ADAMANT HEALTH F2-2007-201342F.R.S.-FNRS - Fonds de la Recherche ScientifiqueF.R.S.-FNRS - Fund for Scientific ResearchTélévieCAC - Centre anticancéreux près l'Université de Liège asblUniversité de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastasesUniversité de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastasesUniversité de Liège - ULiège > Center for Analytical Research and Technology (CART)Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire03q83t159Turtoi, AndreiMusmeci, DavideNaccarato, Antonio GiuseppeScatena, CristianOrtenzi, ValerioKiss, RobertMurtas, DanielaPatsos, GeorgiosMazzucchelli, GabrielDe Pauw, EdwinBevilacqua, GenerosoCastronovo, VincenzoglioblastomacancerproteomicsbiomarkersDSO/A00Giga-Cancer - ULiège0000-0002-8757-81330000-0003-3475-1315High-grade gliomas (glioblastomas) are the most common and deadly brain tumors in adults, currently with no satisfactory treatment available. Apart from de novo glioblastoma, it is currently accepted that these malignancies mainly progress from lower grade glial tumors. However, the molecular entities governing the progression of gliomas are poorly understood. Extracellular and membrane proteins are key biomolecules found at the cell-to-cell communication interface and hence are a promising proteome subpopulation that could help understand the development of glioma. Accordingly, the current study aims at identifying new protein markers of human glioma progression. For this purpose, we used glial tumors generated orthotopically with T98G and U373 human glioma cells in nude mice. This setup allowed also to discriminate the protein origin, namely, human (tumor) or mouse (host). Extracellular and membrane proteins were selectively purified using biotinylation followed by streptavidin affinity chromatography. Isolated proteins were digested and then identified and quantified employing 2D-nano-HPLC-MS/MS analysis. A total of 23 and 27 up-regulated extracellular and membrane proteins were identified in the T98G and U373 models, respectively. Approximately two-thirds of these were predominantly produced by the tumor, whereas the remaining proteins appeared to be mainly overexpressed by the host tissue. Following extensive validation, we have focused our attention on sparc-like protein 1. This protein was further investigated using immunohistochemistry in a large collection of human glioma samples of different grades. The results showed that sparc-like protein 1 expression correlates with glioma grade, suggesting the possible role for this protein in the progression of this malignancy.F00authorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthor22909274Journal of Proteome Research1535-38931535-3907Research Concerted Action (IDEA project) of the University of Liège (ULG)CEE (FP7 network: ADAMANT HEALTH F2-2007-201342F.R.S.-FNRS - Fonds de la Recherche ScientifiqueTélévieCAC - Centre anticancéreux près l'Université de Liège asblenPeer Reviewed verified by ORBiASJC_1600ASJC_1303Giga-Cancer - ULiègeAmerican Chemical Society (ACS)Turtoi, A., Musmeci, D., Naccarato, A. G., Scatena, C., Ortenzi, V., Kiss, R., Murtas, D., Patsos, G., Mazzucchelli, G., De Pauw, E., Bevilacqua, G., & Castronovo, V. (2012). Sparc-like protein 1 is a new marker of human glioma progression. <em>Journal of Proteome Research, 11</em> (10), 5011-21. doi:10.1021/pr3005698p005854p129600p003981p001255p00140910.1021/pr3005698application/pdf2268/1346122024-10-21T21:07:31Zopenalex33OCI222012Article (Scientific journals)Gastroenterology & hepatologyMedicine > HepatologyMedicine > GastroenterologyRestricted accessPeer Reviewed verified by ORBiCentre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-HépatologieCentre Hospitalier Universitaire de Liège - CHU > Centre d'oncologieLouis, EdouardMary, J. Y.Vernier-Massouille, G.Grimaud, J. C.Bouhnik, Y.Laharie, D.Dupas, J. L.Pillant, H.Picon, L.Veyrac, M.Flamant, M.Savoye, G.Jian, R.Devos, MartinePorcher, R.Paintaud, G.Piver, E.Colombel, J. F.Lemann, M.DSO/A000000-0002-1287-7075BACKGROUND & AIMS: It is important to determine whether infliximab therapy can be safely interrupted in patients with Crohn's disease who have undergone a period of prolonged remission. We assessed the risk of relapse after infliximab therapy was discontinued in patients on combined maintenance therapy with antimetabolites and identified factors associated with relapse. METHODS: We performed a prospective study of 115 patients with Crohn's disease who were treated for at least 1 year with scheduled infliximab and an antimetabolite and had been in corticosteroid-free remission for at least 6 months. Infliximab was stopped, and patients were followed up for at least 1 year. We associated demographic, clinical, and biologic factors with time to relapse using a Cox model. RESULTS: After a median follow-up period of 28 months, 52 of the 115 patients experienced a relapse; the 1-year relapse rate was 43.9% +/- 5.0%. Based on multivariable analysis, risk factors for relapse included male sex, the absence of surgical resection, leukocyte counts >6.0 x 10(9)/L, and levels of hemoglobin </=145 g/L, C-reactive protein >/=5.0 mg/L, and fecal calprotectin >/=300 mug/g. Patients with no more than 2 of these risk factors (approximately 29% of the study population) had a 15% risk of relapse within 1 year. Re-treatment with infliximab was effective and well tolerated in 88% of patients who experienced a relapse. CONCLUSIONS: Approximately 50% of patients with Crohn's disease who were treated for at least 1 year with infliximab and an antimetabolite agent experienced a relapse within 1 year after discontinuation of infliximab. However, patients with a low risk of relapse can be identified using a combination of clinical and biologic markers.D00authorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthorauthor21945953Gastroenterology0016-50851528-0012enPeer Reviewed verified by ORBiASJC_2721ASJC_2715SaundersLouis, E., Mary, J. Y., Vernier-Massouille, G., Grimaud, J. C., Bouhnik, Y., Laharie, D., Dupas, J. L., Pillant, H., Picon, L., Veyrac, M., Flamant, M., Savoye, G., Jian, R., DEVOS, M., Porcher, R., Paintaud, G., Piver, E., Colombel, J. F., & Lemann, M. (2012). Maintenance of Remission Among Patients With Crohn's Disease on Antimetabolite Therapy After Infliximab Therapy Is Stopped. <em>Gastroenterology, 142</em> (1), 63-70.e5; quiz e31. doi:10.1053/j.gastro.2011.09.034p002648p00875010.1053/j.gastro.2011.09.034application/pdf2268/1082622024-10-21T18:39:51Zopenalex589OCI4492012Contribution to collective works (Parts of books)Biochemistry, biophysics & molecular biologyOpen AccessPeer reviewedWislet, SabineUniversité de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaineUniversité de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaineUniversité de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaineGlejzer, AnetaNeirinckx, VirginieRogister, BernardWislet, SabineNeural creststem cellsneurodegenerative diseaseAdvance in Regenerative Medicine9789533078380DSO/C000000-0003-1754-60020000-0003-4279-96690000-0003-4279-9669F00authorauthorauthorauthorenPeer reviewedIntech-open access publisherGlejzer, A., Neirinckx, V., Rogister, B., & Wislet, S. (2011). Neural crest stem cells from adult bone marrow: a new source for cell replacement therapy? In S. Wislet (Ed.), <em>Advance in Regenerative Medicine</em> (pp. 3-18). Croatia: Intech-open access publisher. p019624p002681p003397application/pdf2268/1017612024-10-22T11:38:01Z2011Poster (Scientific congresses and symposiums)ZoologyOpen AccessUniversité de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Morphologie fonctionnelle et évolutiveUniversité de Liège - ULiège > Département de physique > Département de physiqueParmentier, EricDragicevic, BCorbisier de Harbonnier de Cobreville, YvesBouillac, GrégoryDulcic, JakovenDSO/D000000-0002-0391-753015th Benelux Congress of ZoologyF00authorauthorauthorauthorauthorParmentier, E., Dragicevic, B., Corbisier de Harbonnier de Cobreville, Y., Bouillac, G., & Dulcic, J. (2008). <em>Preliminary results on the sound production in Ophidion rochai</em> [Poster presentation]. 15th Benelux Congress of Zoology. p003154p005882application/pdf2268/1354912024-10-22T11:43:37Z2008Paper published in a book (Scientific congresses and symposiums)Veterinary medicine & animal healthPeer reviewedUniversité de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animauxDe Vries, H. W.Clercx, Cécilevan den Brom, W. E.enPeer reviewedAdvances in Companion Animal Medicine and surgery - Utrecht - Nederland - septembre 1986DSO/D000000-0002-1421-2413Advances in Companion Animal Medicine and surgeryF00authorauthorauthorDe Vries, H. W., Clercx, C., & van den Brom, W. E. (1986). Radionuclides in living diagnostics, a new approach. In <em>Advances in Companion Animal Medicine and surgery - Utrecht - Nederland - septembre 1986</em>. p0014492268/1355482024-10-22T09:17:30Z1986-09-12Unpublished conference/Abstract (Scientific congresses and symposiums)MicrobiologyUniversité de Liège - ULiège > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaireDelcenserie, VéroniqueenDSO/D000000-0002-8260-7046International Symposium on propionibacteria and bifidobacteria: dairy and probiotic applicationsF00authorDelcenserie, V. (2004). <em>Development of a PCR method for detection of bifidobacteria from raw milk and raw milk cheese: comparison with a cultural method</em> [Paper presentation]. International Symposium on propionibacteria and bifidobacteria: dairy and probiotic applications. p0742252268/1354592024-11-07T04:40:04Z2004Poster (Scientific congresses and symposiums)MicrobiologyUniversité de Liège - ULiège > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaireUniversité de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentairesDelcenserie, Véroniquechina, bernardDaube, GeorgesenDSO/D000000-0002-8260-70460000-0002-9282-8408Food-PCR meetingF00authorauthorauthorDelcenserie, V., china, B., & Daube, G. (2001). <em>Differentiation of Bifidobacterium species by PCR-RFLP</em> [Poster presentation]. Food-PCR meeting. p074225p0015592268/1354652024-11-07T04:40:04Z2001Scientific conference in universities or research centersSociology & social sciencesUniversité de Liège - ULiège > Institut des sciences humaines et sociales > Centre d'études de l'ethnicité et des migrations (CEDEM)Martiniello, MarcofrDSO/M000000-0002-8707-1578Séminaire: " L’intégration des nouveaux migrants en France"H00authorMartiniello, M. (17 March 2008). <em>"Participation des migrants et accès aux Droits."</em> [Paper presentation]. Séminaire: " L’intégration des nouveaux migrants en France", Paris, France. p0021572268/1355652024-10-21T18:06:29Z2008-03-17Scientific conference in universities or research centersSociology & social sciencesUniversité de Liège - ULiège > Institut des sciences humaines et sociales > Centre d'études de l'ethnicité et des migrations (CEDEM)Martiniello, MarcofrDSO/M000000-0002-8707-1578Séminaire doctoral: Migrations, Diasporas, Mobilités.H00authorMartiniello, M. (26 January 2006). <em>Transnationalisme Politique et Citoyenneté</em> [Paper presentation]. Séminaire doctoral: Migrations, Diasporas, Mobilités, Toulouse, France. p0021572268/1355642024-10-21T18:06:29Z2006-01-26Scientific conference in universities or research centersSociology & social sciencesUniversité de Liège - ULiège > Institut des sciences humaines et sociales > Centre d'études de l'ethnicité et des migrations (CEDEM)Martiniello, MarcofrDSO/M000000-0002-8707-1578Colloque: L’intégration des immigrés : Une comparaison Montréal-Bruxelles.H00authorMartiniello, M. (21 February 2006). <em>Conclusions et perspectives critiques</em> [Paper presentation]. Colloque: L’intégration des immigrés : Une comparaison Montréal-Bruxelles, Bruxelles, Belgium. p0021572268/1355692024-10-21T18:06:29Z2006-02-21